UniProtKB - Q8JZN7 (MIRO2_MOUSE)
Mitochondrial Rho GTPase 2
Rhot2
Functioni
Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 18 | GTP 1Sequence analysis | 8 | |
Nucleotide bindingi | 57 – 61 | GTP 1Sequence analysis | 5 | |
Nucleotide bindingi | 118 – 121 | GTP 1Sequence analysis | 4 | |
Calcium bindingi | 197 – 208 | 1PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 317 – 328 | 2PROSITE-ProRule annotationAdd BLAST | 12 | |
Nucleotide bindingi | 424 – 431 | GTP 2Sequence analysis | 8 | |
Nucleotide bindingi | 461 – 465 | GTP 2Sequence analysis | 5 | |
Nucleotide bindingi | 526 – 529 | GTP 2Sequence analysis | 4 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- GTPase activity Source: GO_Central
- GTP binding Source: GO_Central
GO - Biological processi
- cellular homeostasis Source: UniProtKB
- mitochondrial outer membrane permeabilization Source: UniProtKB
- mitochondrion organization Source: GO_Central
- mitochondrion transport along microtubule Source: UniProtKB
- regulation of mitochondrion organization Source: GO_Central
- small GTPase mediated signal transduction Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Ligand | Calcium, GTP-binding, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-9013419, RHOT2 GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial Rho GTPase 2 (EC:3.6.5.-)Short name: MIRO-2 Alternative name(s): Ras homolog gene family member T2 |
Gene namesi | Name:Rhot2 Synonyms:Arht2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2384892, Rhot2 |
VEuPathDBi | HostDB:ENSMUSG00000025733 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane By similarity; Single-pass type IV membrane protein By similarity
Note: Colocalizes with MGARP and RHOT2 at the mitochondria.By similarity
Mitochondrion
- integral component of mitochondrial outer membrane Source: UniProtKB
- mitochondrial inner membrane Source: MGI
- mitochondrion Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 594 | CytoplasmicSequence analysisAdd BLAST | 594 | |
Transmembranei | 595 – 617 | Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 618 – 620 | Mitochondrial intermembraneSequence analysis | 3 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000239319 | 1 – 620 | Mitochondrial Rho GTPase 2Add BLAST | 620 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | Q8JZN7 |
jPOSTi | Q8JZN7 |
MaxQBi | Q8JZN7 |
PaxDbi | Q8JZN7 |
PeptideAtlasi | Q8JZN7 |
PRIDEi | Q8JZN7 |
ProteomicsDBi | 252569 |
PTM databases
iPTMneti | Q8JZN7 |
PhosphoSitePlusi | Q8JZN7 |
SwissPalmi | Q8JZN7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000025733, Expressed in retinal neural layer and 289 other tissues |
ExpressionAtlasi | Q8JZN7, baseline and differential |
Genevisiblei | Q8JZN7, MM |
Interactioni
Subunit structurei
Interacts with the kinesin-binding proteins TRAK1/OIP106 and TRAK2/GRIF1, forming a link between mitochondria and the trafficking apparatus of the microtubules (By similarity).
Interacts with ARMCX3 (PubMed:22569362).
Found in a complex with KIF5B, OGT, RHOT1 and TRAK1 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 229580, 1 interactor |
STRINGi | 10090.ENSMUSP00000044639 |
Miscellaneous databases
RNActi | Q8JZN7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 168 | Miro 1PROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 184 – 219 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 304 – 339 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 415 – 578 | Miro 2PROSITE-ProRule annotationAdd BLAST | 164 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1707, Eukaryota |
GeneTreei | ENSGT00940000158109 |
HOGENOMi | CLU_014255_3_1_1 |
InParanoidi | Q8JZN7 |
OMAi | HYMDSQI |
OrthoDBi | 538388at2759 |
PhylomeDBi | Q8JZN7 |
TreeFami | TF300814 |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR013566, EF_hand_assoc_1 IPR013567, EF_hand_assoc_2 IPR002048, EF_hand_dom IPR021181, Miro IPR020860, MIRO_dom IPR027417, P-loop_NTPase IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
PANTHERi | PTHR24072, PTHR24072, 1 hit |
Pfami | View protein in Pfam PF08355, EF_assoc_1, 1 hit PF08356, EF_assoc_2, 1 hit PF00071, Ras, 1 hit |
PIRSFi | PIRSF037488, Mt_Rho_GTPase, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 2 hits SM00174, RHO, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS51423, MIRO, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MRRDVRILLL GEAQVGKTSL ILSLVGEEFP EEVPARAEEI TIPADVTPEK
60 70 80 90 100
VPTHIVDYSE AEQTEEELQE EIHKANVVCV VYDVSEETTI EKIRTKWIPL
110 120 130 140 150
VNGRTATGPR LPIILVGNKS DLRPGSTMEA VLPIMSQFPE IETCVECSAK
160 170 180 190 200
HLRNISELFY YAQKAVLHPT APLYDPEAKQ LRPACAQALT RIFRLSDQDR
210 220 230 240 250
DHGLSDEELN AFQKSCFGHP LAPQALEDVK RVVCKNVSGG VQNDRLTLEG
260 270 280 290 300
FLFLNTLFIQ RGRHETTWTI LRRFGYSDSL ELTPDYLYPA LHVPPGCSTE
310 320 330 340 350
LNHRGYQFVQ RMFEKHDQDH DGVLSPTELQ NLFSVFSGAP WGPELLHTVP
360 370 380 390 400
TQAGCLPLHG YLCQWTLMTY LDVQQCLAHL GYLGYPTLCE QDSQAQAITV
410 420 430 440 450
TREKKLDQEK GQTQRSVLMC KVLGARGVGK SAFLQAFLGN SLGEARDPPE
460 470 480 490 500
KFPLHTINTV RVNGQEKYLI LCEVNADSLL DTSLDTTCDV ACLMFDSSDP
510 520 530 540 550
KTFVHCATIY KRYYMDGQTP CLFIASKADL PEGVAPPGLS PAEFCRRHRL
560 570 580 590 600
PAPASFSCLG PAMPSTDVFT QLATMATFPH LVHTELHPTS FWLRGVLVAV
610 620
GTAVAAVLSF SLYRVLVKSR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BL14 | H3BL14_MOUSE | Mitochondrial Rho GTPase 2 | Rhot2 | 172 | Annotation score: | ||
H3BKL2 | H3BKL2_MOUSE | Mitochondrial Rho GTPase 2 | Rhot2 | 121 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY311390 mRNA Translation: AAP78906.1 BC029777 mRNA Translation: AAH29777.1 BC034062 mRNA Translation: AAH34062.1 |
CCDSi | CCDS37505.1 |
RefSeqi | NP_666111.1, NM_145999.2 |
Genome annotation databases
Ensembli | ENSMUST00000043897; ENSMUSP00000044639; ENSMUSG00000025733 |
GeneIDi | 214952 |
KEGGi | mmu:214952 |
UCSCi | uc008bch.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY311390 mRNA Translation: AAP78906.1 BC029777 mRNA Translation: AAH29777.1 BC034062 mRNA Translation: AAH34062.1 |
CCDSi | CCDS37505.1 |
RefSeqi | NP_666111.1, NM_145999.2 |
3D structure databases
SMRi | Q8JZN7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229580, 1 interactor |
STRINGi | 10090.ENSMUSP00000044639 |
PTM databases
iPTMneti | Q8JZN7 |
PhosphoSitePlusi | Q8JZN7 |
SwissPalmi | Q8JZN7 |
Proteomic databases
EPDi | Q8JZN7 |
jPOSTi | Q8JZN7 |
MaxQBi | Q8JZN7 |
PaxDbi | Q8JZN7 |
PeptideAtlasi | Q8JZN7 |
PRIDEi | Q8JZN7 |
ProteomicsDBi | 252569 |
Protocols and materials databases
DNASUi | 214952 |
Genome annotation databases
Ensembli | ENSMUST00000043897; ENSMUSP00000044639; ENSMUSG00000025733 |
GeneIDi | 214952 |
KEGGi | mmu:214952 |
UCSCi | uc008bch.1, mouse |
Organism-specific databases
CTDi | 89941 |
MGIi | MGI:2384892, Rhot2 |
VEuPathDBi | HostDB:ENSMUSG00000025733 |
Phylogenomic databases
eggNOGi | KOG1707, Eukaryota |
GeneTreei | ENSGT00940000158109 |
HOGENOMi | CLU_014255_3_1_1 |
InParanoidi | Q8JZN7 |
OMAi | HYMDSQI |
OrthoDBi | 538388at2759 |
PhylomeDBi | Q8JZN7 |
TreeFami | TF300814 |
Enzyme and pathway databases
Reactomei | R-MMU-9013419, RHOT2 GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 214952, 6 hits in 66 CRISPR screens |
ChiTaRSi | Rhot2, mouse |
PROi | PR:Q8JZN7 |
RNActi | Q8JZN7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025733, Expressed in retinal neural layer and 289 other tissues |
ExpressionAtlasi | Q8JZN7, baseline and differential |
Genevisiblei | Q8JZN7, MM |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR013566, EF_hand_assoc_1 IPR013567, EF_hand_assoc_2 IPR002048, EF_hand_dom IPR021181, Miro IPR020860, MIRO_dom IPR027417, P-loop_NTPase IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
PANTHERi | PTHR24072, PTHR24072, 1 hit |
Pfami | View protein in Pfam PF08355, EF_assoc_1, 1 hit PF08356, EF_assoc_2, 1 hit PF00071, Ras, 1 hit |
PIRSFi | PIRSF037488, Mt_Rho_GTPase, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 2 hits SM00174, RHO, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS51423, MIRO, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | MIRO2_MOUSE | |
Accessioni | Q8JZN7Primary (citable) accession number: Q8JZN7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families