UniProtKB - Q8JZM4 (DNER_MOUSE)
Protein
Delta and Notch-like epidermal growth factor-related receptor
Gene
Dner
Organism
Mus musculus (Mouse)
Status
Functioni
Mediates neuron-glia interaction during astrocytogenesis. May promote differentiation of Bergmann glia during cerebellar development by activating DELTEX-dependent NOTCH1 signaling.2 Publications
GO - Molecular functioni
- calcium ion binding Source: InterPro
- Notch binding Source: MGI
GO - Biological processi
- central nervous system development Source: Ensembl
- glial cell differentiation Source: MGI
- Notch receptor processing Source: MGI
- Notch signaling pathway Source: UniProtKB-KW
- skeletal muscle fiber development Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, Receptor |
Biological process | Differentiation, Notch signaling pathway |
Ligand | Calcium |
Names & Taxonomyi
Protein namesi | Recommended name: Delta and Notch-like epidermal growth factor-related receptorAlternative name(s): Brain EGF repeat-containing transmembrane protein |
Gene namesi | Name:Dner Synonyms:Bet, Bret |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2152889, Dner |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Single-pass type I membrane protein 2 Publications
Note: Present on the membrane of dendrites and cell bodies but excluded from axonal membrane. Also found in early endosomes in the somatodendritic region.
Endosome
- early endosome Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- dendrite Source: MGI
- integral component of membrane Source: UniProtKB-KW
- neuronal cell body Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 26 – 640 | ExtracellularSequence analysisAdd BLAST | 615 | |
Transmembranei | 641 – 661 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 662 – 737 | CytoplasmicSequence analysisAdd BLAST | 76 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mice show no obvious abnormality and no apparent survival disadvantage. However, they have delayed cerebellar histogenesis and exhibit motor discoordination at adult stages.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 677 | Y → A: Fails to be restricted to the somatodendritic compartment. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000253558 | 26 – 737 | Delta and Notch-like epidermal growth factor-related receptorAdd BLAST | 712 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 48 ↔ 59 | PROSITE-ProRule annotation | ||
Disulfide bondi | 53 ↔ 80 | PROSITE-ProRule annotation | ||
Disulfide bondi | 82 ↔ 91 | PROSITE-ProRule annotation | ||
Disulfide bondi | 98 ↔ 108 | PROSITE-ProRule annotation | ||
Disulfide bondi | 103 ↔ 121 | PROSITE-ProRule annotation | ||
Disulfide bondi | 123 ↔ 132 | PROSITE-ProRule annotation | ||
Glycosylationi | 204 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 319 ↔ 336 | PROSITE-ProRule annotation | ||
Disulfide bondi | 338 ↔ 347 | PROSITE-ProRule annotation | ||
Disulfide bondi | 353 ↔ 364 | PROSITE-ProRule annotation | ||
Disulfide bondi | 358 ↔ 378 | PROSITE-ProRule annotation | ||
Disulfide bondi | 380 ↔ 389 | PROSITE-ProRule annotation | ||
Disulfide bondi | 396 ↔ 407 | PROSITE-ProRule annotation | ||
Disulfide bondi | 401 ↔ 416 | PROSITE-ProRule annotation | ||
Disulfide bondi | 418 ↔ 427 | PROSITE-ProRule annotation | ||
Disulfide bondi | 434 ↔ 445 | PROSITE-ProRule annotation | ||
Disulfide bondi | 439 ↔ 454 | PROSITE-ProRule annotation | ||
Disulfide bondi | 456 ↔ 465 | PROSITE-ProRule annotation | ||
Disulfide bondi | 472 ↔ 482 | PROSITE-ProRule annotation | ||
Disulfide bondi | 477 ↔ 491 | PROSITE-ProRule annotation | ||
Disulfide bondi | 493 ↔ 502 | PROSITE-ProRule annotation | ||
Disulfide bondi | 509 ↔ 520 | PROSITE-ProRule annotation | ||
Disulfide bondi | 514 ↔ 529 | PROSITE-ProRule annotation | ||
Disulfide bondi | 531 ↔ 540 | PROSITE-ProRule annotation | ||
Disulfide bondi | 547 ↔ 558 | PROSITE-ProRule annotation | ||
Disulfide bondi | 552 ↔ 567 | PROSITE-ProRule annotation | ||
Glycosylationi | 564 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 569 ↔ 578 | PROSITE-ProRule annotation | ||
Disulfide bondi | 585 ↔ 596 | PROSITE-ProRule annotation | ||
Disulfide bondi | 590 ↔ 605 | PROSITE-ProRule annotation | ||
Disulfide bondi | 607 ↔ 616 | PROSITE-ProRule annotation | ||
Modified residuei | 685 | PhosphoserineCombined sources | 1 | |
Modified residuei | 711 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 714 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 721 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 722 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | Q8JZM4 |
PaxDbi | Q8JZM4 |
PeptideAtlasi | Q8JZM4 |
PRIDEi | Q8JZM4 |
PTM databases
GlyConnecti | 2251, 4 N-Linked glycans (2 sites) |
GlyGeni | Q8JZM4, 4 sites |
iPTMneti | Q8JZM4 |
PhosphoSitePlusi | Q8JZM4 |
Expressioni
Tissue specificityi
Specifically expressed in brain neurons (at protein level).2 Publications
Developmental stagei
Expression in the central nervous system starts at 11 dpc, peaks during postnatal development and declines in the adult brain. At P7 and P20, present in several types of post-mitotic neurons, including cortical and hippocampal pyramidal neurons, cerebellar granule cells and Purkinje cells. Absent from mitotic neuroblasts in the ventricular zones.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000036766, Expressed in cerebellum lobe and 254 other tissues |
Genevisiblei | Q8JZM4, MM |
Interactioni
Subunit structurei
Interacts with AP1G1.
Interacts with NOTCH1.
2 PublicationsGO - Molecular functioni
- Notch binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230612, 1 interactor |
STRINGi | 10090.ENSMUSP00000042927 |
Miscellaneous databases
RNActi | Q8JZM4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 92 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 49 | |
Domaini | 94 – 133 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 309 – 348 | EGF-like 3PROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 349 – 390 | EGF-like 4PROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 392 – 428 | EGF-like 5PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 430 – 466 | EGF-like 6PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 468 – 503 | EGF-like 7PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 505 – 541 | EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 543 – 579 | EGF-like 9PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 581 – 617 | EGF-like 10; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 44 – 133 | Interaction with NOTCH11 PublicationAdd BLAST | 90 | |
Regioni | 677 – 680 | Interaction with AP1G1 and somatodendritic targeting1 Publication | 4 |
Keywords - Domaini
EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000158872 |
HOGENOMi | CLU_019513_0_0_1 |
InParanoidi | Q8JZM4 |
OMAi | CLDQSNG |
OrthoDBi | 7525at2759 |
PhylomeDBi | Q8JZM4 |
TreeFami | TF351322 |
Family and domain databases
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf |
Pfami | View protein in Pfam PF00008, EGF, 6 hits PF12661, hEGF, 1 hit |
SMARTi | View protein in SMART SM00181, EGF, 10 hits SM00179, EGF_CA, 7 hits |
SUPFAMi | SSF57184, SSF57184, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 2 hits PS00022, EGF_1, 10 hits PS01186, EGF_2, 7 hits PS50026, EGF_3, 10 hits PS01187, EGF_CA, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8JZM4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPRRAQAPG APLLPVLALL PLLLGAGPQS GCLASPVSAA PLPAPGPCAS
60 70 80 90 100
QPCRNGGVCT PRSVTDQEHP AADAEPRYSC TCPAGVSGTY CQFVADPCAS
110 120 130 140 150
NPCHHGNCSS SSSSSSDSYL CICNDGYEGL NCEQPLPSIP TSGWTESTAP
160 170 180 190 200
RQLQPVPATQ EPDIILPRSQ ATVTLPTWQP KTGQKVVEMK WDQVEVVPDV
210 220 230 240 250
ACGNASSNNS AGGRLVSFEV PQNTSVKIRQ DANSLLILLW KVTATGFQQC
260 270 280 290 300
SLIDGRSVTP LQAPGGLVLL EEMLALGPNH FIGFVNDSVA KSIVALRLTL
310 320 330 340 350
VVKASNCVPG DSHSNDLECS GKGKCATKPS EATFSCTCQD QYIGTFCEEF
360 370 380 390 400
DACQRKPCQN EASCIDANEK QDGSNFTCLC LPGYTGELCQ SKIDYCVLDP
410 420 430 440 450
CRNGATCVSS LSGFTCQCLE GYFGSACEEK VDPCMSSPCQ NNGTCYVDGV
460 470 480 490 500
HFTCSCSPGF TGPTCAQLVD FCALSPCAHG MCRSVGTSYK CLCDPGYHGL
510 520 530 540 550
YCEEEYNECL SAPCLNAATC RDLINGYECV CLAEYKGTHC ELYKDPCANI
560 570 580 590 600
SCLNGGTCDS EGLNGTCICA PGFTGEECDI DINECDSNPC HHAGTCLDQP
610 620 630 640 650
NGYTCHCPHG WVGANCEIHL QWKSGHMAES LTNMPRHSLY IIIGALCVAF
660 670 680 690 700
ILMLIILIVG ICRISRIEYQ GSSRPAYEEF YNCRSIDSEF SNAIASIRHA
710 720 730
RFGKKSRPAM YDVTPIAYED YSPDDKPLVT LIKTKDL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WSC4 | A0A087WSC4_MOUSE | Delta and Notch-like epidermal grow... | Dner | 175 | Annotation score: | ||
A0A087WRJ8 | A0A087WRJ8_MOUSE | Delta and Notch-like epidermal grow... | Dner | 38 | Annotation score: |
Sequence cautioni
The sequence BAE31470 differs from that shown. Reason: Frameshift.Curated
The sequence BAE31828 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 424 | G → A in BAB72175 (PubMed:11997712).Curated | 1 | |
Sequence conflicti | 509 – 510 | CL → SV in AAM14419 (PubMed:11950833).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY032924 mRNA Translation: AAK50342.1 AF370126 mRNA Translation: AAM14419.1 AB067650 mRNA Translation: BAB72175.1 AK044597 mRNA Translation: BAC31995.1 AK152755 mRNA Translation: BAE31470.1 Frameshift. AK153235 mRNA Translation: BAE31828.1 Frameshift. BC022636 mRNA Translation: AAH22636.1 BC034634 mRNA Translation: AAH34634.1 |
CCDSi | CCDS15105.1 |
RefSeqi | NP_690879.1, NM_152915.1 |
Genome annotation databases
Ensembli | ENSMUST00000049126; ENSMUSP00000042927; ENSMUSG00000036766 |
GeneIDi | 227325 |
KEGGi | mmu:227325 |
UCSCi | uc007bsw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY032924 mRNA Translation: AAK50342.1 AF370126 mRNA Translation: AAM14419.1 AB067650 mRNA Translation: BAB72175.1 AK044597 mRNA Translation: BAC31995.1 AK152755 mRNA Translation: BAE31470.1 Frameshift. AK153235 mRNA Translation: BAE31828.1 Frameshift. BC022636 mRNA Translation: AAH22636.1 BC034634 mRNA Translation: AAH34634.1 |
CCDSi | CCDS15105.1 |
RefSeqi | NP_690879.1, NM_152915.1 |
3D structure databases
SMRi | Q8JZM4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230612, 1 interactor |
STRINGi | 10090.ENSMUSP00000042927 |
PTM databases
GlyConnecti | 2251, 4 N-Linked glycans (2 sites) |
GlyGeni | Q8JZM4, 4 sites |
iPTMneti | Q8JZM4 |
PhosphoSitePlusi | Q8JZM4 |
Proteomic databases
MaxQBi | Q8JZM4 |
PaxDbi | Q8JZM4 |
PeptideAtlasi | Q8JZM4 |
PRIDEi | Q8JZM4 |
Protocols and materials databases
Antibodypediai | 2715, 220 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000049126; ENSMUSP00000042927; ENSMUSG00000036766 |
GeneIDi | 227325 |
KEGGi | mmu:227325 |
UCSCi | uc007bsw.1, mouse |
Organism-specific databases
CTDi | 92737 |
MGIi | MGI:2152889, Dner |
Phylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000158872 |
HOGENOMi | CLU_019513_0_0_1 |
InParanoidi | Q8JZM4 |
OMAi | CLDQSNG |
OrthoDBi | 7525at2759 |
PhylomeDBi | Q8JZM4 |
TreeFami | TF351322 |
Miscellaneous databases
BioGRID-ORCSi | 227325, 0 hits in 17 CRISPR screens |
ChiTaRSi | Dner, mouse |
PROi | PR:Q8JZM4 |
RNActi | Q8JZM4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036766, Expressed in cerebellum lobe and 254 other tissues |
Genevisiblei | Q8JZM4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf |
Pfami | View protein in Pfam PF00008, EGF, 6 hits PF12661, hEGF, 1 hit |
SMARTi | View protein in SMART SM00181, EGF, 10 hits SM00179, EGF_CA, 7 hits |
SUPFAMi | SSF57184, SSF57184, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 2 hits PS00022, EGF_1, 10 hits PS01186, EGF_2, 7 hits PS50026, EGF_3, 10 hits PS01187, EGF_CA, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DNER_MOUSE | |
Accessioni | Q8JZM4Primary (citable) accession number: Q8JZM4 Secondary accession number(s): Q3U697 Q8VD97 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot