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UniProtKB - Q8JZL3 (THTPA_MOUSE)
Protein
Thiamine-triphosphatase
Gene
Thtpa
Organism
Mus musculus (Mouse)
Status
Functioni
Hydrolase highly specific for thiamine triphosphate (ThTP).
1 PublicationCatalytic activityi
- EC:3.6.1.281 Publication
Cofactori
Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication
pH dependencei
Optimum pH is 7.5-9.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 7 | Magnesium | 1 | |
Metal bindingi | 9 | Magnesium | 1 | |
Binding sitei | 11 | Substrate | 1 | |
Binding sitei | 55 | Substrate | 1 | |
Binding sitei | 57 | Substrate | 1 | |
Binding sitei | 65 | Substrate | 1 | |
Binding sitei | 125 | Substrate | 1 | |
Metal bindingi | 145 | Magnesium | 1 | |
Metal bindingi | 157 | Magnesium | 1 | |
Binding sitei | 157 | SubstrateBy similarity | 1 | |
Metal bindingi | 159 | Magnesium | 1 | |
Binding sitei | 193 | Substrate | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB
- thiamin-triphosphatase activity Source: UniProtKB
GO - Biological processi
- dephosphorylation Source: MGI
- thiamine diphosphate metabolic process Source: UniProtKB
- thiamine metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.6.1.28, 3474 |
Reactomei | R-MMU-196819, Vitamin B1 (thiamin) metabolism |
SABIO-RKi | Q8JZL3 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Thtpa |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2446078, Thtpa |
VEuPathDBi | HostDB:ENSMUSG00000045691 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000221492 | 2 – 224 | Thiamine-triphosphataseAdd BLAST | 223 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q8JZL3 |
MaxQBi | Q8JZL3 |
PaxDbi | Q8JZL3 |
PeptideAtlasi | Q8JZL3 |
PRIDEi | Q8JZL3 |
ProteomicsDBi | 259186 |
PTM databases
PhosphoSitePlusi | Q8JZL3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000045691, Expressed in floor plate of midbrain and 291 other tissues |
Genevisiblei | Q8JZL3, MM |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000056026 |
Miscellaneous databases
RNActi | Q8JZL3, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q8JZL3 |
SMRi | Q8JZL3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8JZL3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 201 | CYTHPROSITE-ProRule annotationAdd BLAST | 197 |
Sequence similaritiesi
Belongs to the ThTPase family.Curated
Phylogenomic databases
eggNOGi | ENOG502S5G9, Eukaryota |
GeneTreei | ENSGT00390000005996 |
HOGENOMi | CLU_105907_0_0_1 |
InParanoidi | Q8JZL3 |
OMAi | LMRADHW |
OrthoDBi | 1453157at2759 |
PhylomeDBi | Q8JZL3 |
TreeFami | TF333398 |
Family and domain databases
CDDi | cd07758, ThTPase, 1 hit |
InterProi | View protein in InterPro IPR033469, CYTH-like_dom_sf IPR023577, CYTH_domain IPR039582, THTPA IPR012177, ThTPase_euk |
PANTHERi | PTHR14586, PTHR14586, 1 hit |
Pfami | View protein in Pfam PF01928, CYTH, 1 hit |
PIRSFi | PIRSF036561, ThTPase, 1 hit |
SMARTi | View protein in SMART SM01118, CYTH, 1 hit |
SUPFAMi | SSF55154, SSF55154, 1 hit |
PROSITEi | View protein in PROSITE PS51707, CYTH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8JZL3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQGLIEVER KFAPGPDTEE RLQELGATLE HRVTFRDTYY DTSELSLMLS
60 70 80 90 100
DHWLRQREGS GWELKCPGVT GVSGPHNEYV EVTSEAAIVA QLFELLGSGE
110 120 130 140 150
QKPAGVAAVL GSLKLQEVAS FITTRSSWKL ALSGAHGQEP QLTIDLDSAD
160 170 180 190 200
FGYAVGEVEA MVHEKAEVPA ALEKIITVSS MLGVPAQEEA PAKLMVYLQR
210 220
FRPLDYQRLL EAASSGEATG DSAS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF432864 mRNA Translation: AAM22405.1 AK132479 mRNA Translation: BAE21189.1 BC025562 mRNA Translation: AAH25562.1 |
CCDSi | CCDS27108.1 |
RefSeqi | NP_694723.1, NM_153083.5 |
Genome annotation databases
Ensembli | ENSMUST00000050575; ENSMUSP00000056026; ENSMUSG00000045691 |
GeneIDi | 105663 |
KEGGi | mmu:105663 |
UCSCi | uc007tyb.2, mouse |
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF432864 mRNA Translation: AAM22405.1 AK132479 mRNA Translation: BAE21189.1 BC025562 mRNA Translation: AAH25562.1 |
CCDSi | CCDS27108.1 |
RefSeqi | NP_694723.1, NM_153083.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JMU | NMR | - | A | 2-224 | [»] | |
5A64 | X-ray | 2.10 | A/B | 1-224 | [»] | |
5A65 | X-ray | 1.98 | A/B | 3-215 | [»] | |
BMRBi | Q8JZL3 | |||||
SMRi | Q8JZL3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000056026 |
PTM databases
PhosphoSitePlusi | Q8JZL3 |
Proteomic databases
EPDi | Q8JZL3 |
MaxQBi | Q8JZL3 |
PaxDbi | Q8JZL3 |
PeptideAtlasi | Q8JZL3 |
PRIDEi | Q8JZL3 |
ProteomicsDBi | 259186 |
Protocols and materials databases
Antibodypediai | 55506, 200 antibodies from 23 providers |
DNASUi | 105663 |
Genome annotation databases
Ensembli | ENSMUST00000050575; ENSMUSP00000056026; ENSMUSG00000045691 |
GeneIDi | 105663 |
KEGGi | mmu:105663 |
UCSCi | uc007tyb.2, mouse |
Organism-specific databases
CTDi | 79178 |
MGIi | MGI:2446078, Thtpa |
VEuPathDBi | HostDB:ENSMUSG00000045691 |
Phylogenomic databases
eggNOGi | ENOG502S5G9, Eukaryota |
GeneTreei | ENSGT00390000005996 |
HOGENOMi | CLU_105907_0_0_1 |
InParanoidi | Q8JZL3 |
OMAi | LMRADHW |
OrthoDBi | 1453157at2759 |
PhylomeDBi | Q8JZL3 |
TreeFami | TF333398 |
Enzyme and pathway databases
BRENDAi | 3.6.1.28, 3474 |
Reactomei | R-MMU-196819, Vitamin B1 (thiamin) metabolism |
SABIO-RKi | Q8JZL3 |
Miscellaneous databases
BioGRID-ORCSi | 105663, 0 hits in 63 CRISPR screens |
ChiTaRSi | Thtpa, mouse |
EvolutionaryTracei | Q8JZL3 |
PROi | PR:Q8JZL3 |
RNActi | Q8JZL3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000045691, Expressed in floor plate of midbrain and 291 other tissues |
Genevisiblei | Q8JZL3, MM |
Family and domain databases
CDDi | cd07758, ThTPase, 1 hit |
InterProi | View protein in InterPro IPR033469, CYTH-like_dom_sf IPR023577, CYTH_domain IPR039582, THTPA IPR012177, ThTPase_euk |
PANTHERi | PTHR14586, PTHR14586, 1 hit |
Pfami | View protein in Pfam PF01928, CYTH, 1 hit |
PIRSFi | PIRSF036561, ThTPase, 1 hit |
SMARTi | View protein in SMART SM01118, CYTH, 1 hit |
SUPFAMi | SSF55154, SSF55154, 1 hit |
PROSITEi | View protein in PROSITE PS51707, CYTH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | THTPA_MOUSE | |
Accessioni | Q8JZL3Primary (citable) accession number: Q8JZL3 Secondary accession number(s): Q3V1G2, Q8C3P9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families