UniProtKB - Q8JSP9 (HEMA_CVPIA)
Protein
Hemagglutinin-esterase
Gene
HE
Organism
Porcine hemagglutinating encephalomyelitis virus (strain IAF-404) (HEV)
Status
Functioni
Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.UniRule annotation
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 40 | NucleophileUniRule annotation | 1 | |
Active sitei | 326 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 329 | Charge relay systemUniRule annotation | 1 |
GO - Molecular functioni
- host cell surface receptor binding Source: UniProtKB-UniRule
- sialate 4-O-acetylesterase activity Source: UniProtKB-EC
- sialate 9-O-acetylesterase activity Source: UniProtKB-EC
- sialate O-acetylesterase activity Source: UniProtKB
GO - Biological processi
- fusion of virus membrane with host plasma membrane Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hemagglutinin, Hydrolase |
Names & Taxonomyi
Protein namesi | Recommended name: Hemagglutinin-esteraseUniRule annotation (EC:3.1.1.53UniRule annotation)Short name: HE proteinUniRule annotation Alternative name(s): E3 glycoproteinUniRule annotation |
Gene namesi | Name:HEUniRule annotation |
Organismi | Porcine hemagglutinating encephalomyelitis virus (strain IAF-404) (HEV) |
Taxonomic identifieri | 230236 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus › |
Virus hosti | Sus scrofa (Pig) [TaxID: 9823] |
Proteomesi |
|
Subcellular locationi
- Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
- Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 17 – 392 | Virion surfaceUniRule annotationAdd BLAST | 376 | |
Transmembranei | 393 – 413 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 414 – 424 | IntravirionUniRule annotationAdd BLAST | 11 |
GO - Cellular componenti
- host cell plasma membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-UniRule
- viral envelope Source: UniProtKB-UniRule
- virion membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cell membrane, Host membrane, Membrane, Viral envelope protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | UniRule annotationAdd BLAST | 16 | |
ChainiPRO_0000037150 | 17 – 424 | Hemagglutinin-esteraseUniRule annotationAdd BLAST | 408 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 44 ↔ 65 | UniRule annotation | ||
Glycosylationi | 54 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Glycosylationi | 89 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Disulfide bondi | 113 ↔ 162 | UniRule annotation | ||
Glycosylationi | 153 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Disulfide bondi | 197 ↔ 276 | UniRule annotation | ||
Disulfide bondi | 205 ↔ 249 | UniRule annotation | ||
Glycosylationi | 236 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Glycosylationi | 301 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Disulfide bondi | 307 ↔ 312 | UniRule annotation | ||
Glycosylationi | 316 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 | |
Disulfide bondi | 347 ↔ 371 | UniRule annotation | ||
Glycosylationi | 358 | N-linked (GlcNAc...) asparagine; by hostUniRule annotation | 1 |
Post-translational modificationi
N-glycosylated in the host RER.UniRule annotation
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Homodimer; disulfide-linked.
Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 7 – 127 | Esterase domain 1UniRule annotationAdd BLAST | 121 | |
Regioni | 128 – 266 | Receptor bindingUniRule annotationAdd BLAST | 139 | |
Regioni | 267 – 379 | Esterase domain 2UniRule annotationAdd BLAST | 113 |
Sequence similaritiesi
Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.UniRule annotation
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
HAMAPi | MF_04207, BETA_CORONA_HE, 1 hit |
InterProi | View protein in InterPro IPR008980, Capsid_hemagglutn IPR042545, HEMA IPR007142, Hemagglutn-estrase_core IPR003860, Hemagglutn-estrase_hemagglutn |
Pfami | View protein in Pfam PF03996, Hema_esterase, 1 hit PF02710, Hema_HEFG, 1 hit |
SUPFAMi | SSF49818, SSF49818, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8JSP9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFLLPRFCLV CSIISTFGFQ NPPTNVVSHF NDDWFLFGDS RTDCNHVVKT
60 70 80 90 100
NPRNYSYMDL NPALCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY
110 120 130 140 150
EGVNFTPYHA FKCTSVGNND IWMQNKGLFY TQVYKKMAVY RSLTLVNVPY
160 170 180 190 200
VYNGSAQPTA LCKSGSLILN NPAYIAREAN VGDYYYKSEA DFSLSGCDEY
210 220 230 240 250
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH
260 270 280 290 300
YLVLPSGNYL AISNELLLTV PTKAICLNKR KVFTPVQVVD SRWNNARQSD
310 320 330 340 350
NMTAVACQLP YCYFRNSTSN YVGIYDVNHG DAGFTSILSG LLYDSPCFSQ
360 370 380 390 400
QGVFRYDNVS TVWPLFPFGN CPTAASIISS DLPICVYDPL PIILLGILLG
410 420
VAVIVIVVLL LYFMVDNGIR QHYA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF481863 Genomic RNA Translation: AAM76999.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF481863 Genomic RNA Translation: AAM76999.1 |
3D structure databases
SMRi | Q8JSP9 |
ModBasei | Search... |
Family and domain databases
HAMAPi | MF_04207, BETA_CORONA_HE, 1 hit |
InterProi | View protein in InterPro IPR008980, Capsid_hemagglutn IPR042545, HEMA IPR007142, Hemagglutn-estrase_core IPR003860, Hemagglutn-estrase_hemagglutn |
Pfami | View protein in Pfam PF03996, Hema_esterase, 1 hit PF02710, Hema_HEFG, 1 hit |
SUPFAMi | SSF49818, SSF49818, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HEMA_CVPIA | |
Accessioni | Q8JSP9Primary (citable) accession number: Q8JSP9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 16, 2003 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families