UniProtKB - Q8IYV9 (IZUM1_HUMAN)
Izumo sperm-egg fusion protein 1
IZUMO1
Functioni
Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg (PubMed:15759005).
The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion (PubMed:15759005).
The ligand-receptor interaction probably does not act as a membrane 'fusogen' (PubMed:15759005).
1 PublicationMiscellaneous
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein binding involved in heterotypic cell-cell adhesion Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- signaling receptor binding Source: UniProtKB
GO - Biological processi
- cell adhesion Source: HGNC-UCL
- fusion of sperm to egg plasma membrane involved in single fertilization Source: HGNC-UCL
- heterotypic cell-cell adhesion Source: UniProtKB
- single fertilization Source: UniProtKB
- sperm-egg recognition Source: UniProtKB
Keywordsi
Biological process | Fertilization |
Enzyme and pathway databases
PathwayCommonsi | Q8IYV9 |
Reactomei | R-HSA-1300645, Acrosome Reaction and Sperm:Oocyte Membrane Binding |
SignaLinki | Q8IYV9 |
Protein family/group databases
TCDBi | 8.A.55.1.1, the izumo sperm-egg fusion protein 1 (izumo1) family |
Names & Taxonomyi
Protein namesi | Recommended name: Izumo sperm-egg fusion protein 1CuratedAlternative name(s): Oocyte binding/fusion factor1 Publication Short name: OBF1 Publication Sperm-specific protein izumo1 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28539, IZUMO1 |
MIMi | 609278, gene |
neXtProti | NX_Q8IYV9 |
VEuPathDBi | HostDB:ENSG00000182264 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Other locations
- acrosome membrane By similarity; Single-pass type I membrane protein Sequence analysis
Note: Localizes initially to the acrosome membrane of the sperm head (both outer and inner acrosomal membranes) (By similarity). During the acrosome reaction, translocates to the plasma membrane (PubMed:15759005).By similarity1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- acrosomal membrane Source: UniProtKB
- integral component of membrane Source: HGNC-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 292 | ExtracellularSequence analysisAdd BLAST | 271 | |
Transmembranei | 293 – 313 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 314 – 350 | CytoplasmicSequence analysisAdd BLAST | 37 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 71 | E → A or K: Mildly decreases interaction with IZUMO1R. 1 Publication | 1 | |
Mutagenesisi | 148 | W → A: Abolishes interaction with IZUMO1R. 1 Publication | 1 | |
Mutagenesisi | 157 | H → A: Nearly abolishes interaction with IZUMO1R. 1 Publication | 1 | |
Mutagenesisi | 160 | R → A or E: Nearly abolishes interaction with IZUMO1R. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 284359 |
OpenTargetsi | ENSG00000182264 |
PharmGKBi | PA142671647 |
Miscellaneous databases
Pharosi | Q8IYV9, Tbio |
Genetic variation databases
BioMutai | IZUMO1 |
DMDMi | 296434545 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000045482 | 22 – 350 | Izumo sperm-egg fusion protein 1Add BLAST | 329 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 22 ↔ 149 | Combined sources2 Publications | ||
Disulfide bondi | 25 ↔ 152 | Combined sources2 Publications | ||
Disulfide bondi | 135 ↔ 159 | Combined sources2 Publications | ||
Disulfide bondi | 139 ↔ 165 | Combined sources2 Publications | ||
Disulfide bondi | 182 ↔ 233 | PROSITE-ProRule annotationCombined sources2 Publications | ||
Glycosylationi | 204 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 325 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
MassIVEi | Q8IYV9 |
PaxDbi | Q8IYV9 |
PeptideAtlasi | Q8IYV9 |
PRIDEi | Q8IYV9 |
ProteomicsDBi | 71246 [Q8IYV9-1] 71247 [Q8IYV9-2] |
PTM databases
GlyGeni | Q8IYV9, 1 site |
iPTMneti | Q8IYV9 |
PhosphoSitePlusi | Q8IYV9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000182264, Expressed in testis and 106 other tissues |
ExpressionAtlasi | Q8IYV9, baseline and differential |
Genevisiblei | Q8IYV9, HS |
Organism-specific databases
HPAi | ENSG00000182264, Tissue enriched (testis) |
Interactioni
Subunit structurei
Monomer (PubMed:27309808, PubMed:27309818). Monomer, homodimer and homooligomer; depending on the context (By similarity).
Interacts with IZUMO1R/JUNO. IZUMO1 and IZUMO1R/JUNO form a complex with 1:1 stoichiometry (PubMed:27309808, PubMed:27309818). In gamete recognition, IZUMO1R/JUNO first binds to monomeric IZUMO1 (By similarity). The weak, but specific interaction with IZUMO1R/JUNO induces IZUMO1 homodimerization (By similarity). The process follows a tight binding phase where IZUMO1 bends the entire structure towards the sperm membrane side through a thiol-disulfide exchange reaction (By similarity). The molecule no longer binds to IZUMO1R/JUNO and instead binds to a putative second oocyte receptor (By similarity).
Interacts with ACE3 (By similarity).
By similarity2 PublicationsBinary interactionsi
IZUMO1 - isoform 1 [Q8IYV9-1]
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-16208297,EBI-16208297 |
IZUMO1R - isoform 1 [A6ND01-1] | 13 | EBI-16208297,EBI-16208304 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein binding involved in heterotypic cell-cell adhesion Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 129843, 28 interactors |
CORUMi | Q8IYV9 |
DIPi | DIP-62033N |
IntActi | Q8IYV9, 21 interactors |
STRINGi | 9606.ENSP00000327786 |
Miscellaneous databases
RNActi | Q8IYV9, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8IYV9 |
SMRi | Q8IYV9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 167 – 251 | Ig-like C2-typePROSITE-ProRule annotationAdd BLAST | 85 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 148 – 160 | Important for interaction with IZUMO1R1 PublicationAdd BLAST | 13 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SFD8, Eukaryota |
GeneTreei | ENSGT00390000015014 |
HOGENOMi | CLU_044481_0_0_1 |
InParanoidi | Q8IYV9 |
OMAi | PATIINF |
OrthoDBi | 813463at2759 |
PhylomeDBi | Q8IYV9 |
TreeFami | TF338356 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR029389, IZUMO IPR032699, Izumo-Ig IPR032700, IZUMO1 |
PANTHERi | PTHR35540, PTHR35540, 1 hit |
Pfami | View protein in Pfam PF15005, IZUMO, 1 hit PF16706, Izumo-Ig, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGPHFTLLCA ALAGCLLPAE GCVICDPSVV LALKSLEKDY LPGHLDAKHH
60 70 80 90 100
KAMMERVENA VKDFQELSLN EDAYMGVVDE ATLQKGSWSL LKDLKRITDS
110 120 130 140 150
DVKGDLFVKE LFWMLHLQKE TFATYVARFQ KEAYCPNKCG VMLQTLIWCK
160 170 180 190 200
NCKKEVHACR KSYDCGERNV EVPQMEDMIL DCELNWHQAS EGLTDYSFYR
210 220 230 240 250
VWGNNTETLV SKGKEATLTK PMVGPEDAGS YRCELGSVNS SPATIINFHV
260 270 280 290 300
TVLPKMIKEE KPSPNIVTPG EATTESSISL QPLQPEKMLA SRLLGLLICG
310 320 330 340 350
SLALITGLTF AIFRRRKVID FIKSSLFGLG SGAAEQTQVP KEKATDSRQQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM0R0B8 | M0R0B8_HUMAN | Izumo sperm-egg fusion protein 1 | IZUMO1 | 15 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 | L → P in AAS59145 (Ref. 2) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055399 | 333 | A → V3 PublicationsCorresponds to variant dbSNP:rs2307019Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016960 | 57 – 68 | VENAV…FQELS → MRPHCKRGPGVC in isoform 3. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_016961 | 69 – 350 | Missing in isoform 3. 1 PublicationAdd BLAST | 282 | |
Alternative sequenceiVSP_016962 | 167 – 194 | ERNVE…SEGLT → GERTGPSRRNWCRAECGSSS NGRHDPGL in isoform 2. 1 PublicationAdd BLAST | 28 | |
Alternative sequenceiVSP_016963 | 195 – 350 | Missing in isoform 2. 1 PublicationAdd BLAST | 156 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB195682 mRNA Translation: BAD91012.1 AY552609 mRNA Translation: AAS59145.1 AY552610 mRNA Translation: AAS59146.1 AC009002 Genomic DNA No translation available. BC034769 mRNA Translation: AAH34769.1 |
CCDSi | CCDS12732.1 [Q8IYV9-1] |
RefSeqi | NP_001308794.1, NM_001321865.1 NP_872381.2, NM_182575.2 [Q8IYV9-1] XP_011525114.1, XM_011526812.2 XP_011525115.1, XM_011526813.2 |
Genome annotation databases
Ensembli | ENST00000332955.7; ENSP00000327786.2; ENSG00000182264.9 ENST00000595517.5; ENSP00000471815.1; ENSG00000182264.9 [Q8IYV9-3] ENST00000595937.5; ENSP00000470144.1; ENSG00000182264.9 [Q8IYV9-2] |
GeneIDi | 284359 |
KEGGi | hsa:284359 |
MANE-Selecti | ENST00000332955.7; ENSP00000327786.2; NM_182575.3; NP_872381.2 |
UCSCi | uc002pkj.3, human [Q8IYV9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB195682 mRNA Translation: BAD91012.1 AY552609 mRNA Translation: AAS59145.1 AY552610 mRNA Translation: AAS59146.1 AC009002 Genomic DNA No translation available. BC034769 mRNA Translation: AAH34769.1 |
CCDSi | CCDS12732.1 [Q8IYV9-1] |
RefSeqi | NP_001308794.1, NM_001321865.1 NP_872381.2, NM_182575.2 [Q8IYV9-1] XP_011525114.1, XM_011526812.2 XP_011525115.1, XM_011526813.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5F4E | X-ray | 2.40 | A | 22-254 | [»] | |
5F4T | X-ray | 3.08 | A | 22-254 | [»] | |
5F4V | X-ray | 2.90 | A | 22-268 | [»] | |
5JK9 | X-ray | 2.10 | A/B/C/D/E/F | 22-255 | [»] | |
5JKC | X-ray | 2.90 | A | 22-255 | [»] | |
5JKD | X-ray | 2.90 | A | 22-255 | [»] | |
5JKE | X-ray | 2.86 | A/C | 22-255 | [»] | |
AlphaFoldDBi | Q8IYV9 | |||||
SMRi | Q8IYV9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 129843, 28 interactors |
CORUMi | Q8IYV9 |
DIPi | DIP-62033N |
IntActi | Q8IYV9, 21 interactors |
STRINGi | 9606.ENSP00000327786 |
Protein family/group databases
TCDBi | 8.A.55.1.1, the izumo sperm-egg fusion protein 1 (izumo1) family |
PTM databases
GlyGeni | Q8IYV9, 1 site |
iPTMneti | Q8IYV9 |
PhosphoSitePlusi | Q8IYV9 |
Genetic variation databases
BioMutai | IZUMO1 |
DMDMi | 296434545 |
Proteomic databases
MassIVEi | Q8IYV9 |
PaxDbi | Q8IYV9 |
PeptideAtlasi | Q8IYV9 |
PRIDEi | Q8IYV9 |
ProteomicsDBi | 71246 [Q8IYV9-1] 71247 [Q8IYV9-2] |
Protocols and materials databases
ABCDi | Q8IYV9, 9 sequenced antibodies |
Antibodypediai | 31796, 137 antibodies from 22 providers |
DNASUi | 284359 |
Genome annotation databases
Ensembli | ENST00000332955.7; ENSP00000327786.2; ENSG00000182264.9 ENST00000595517.5; ENSP00000471815.1; ENSG00000182264.9 [Q8IYV9-3] ENST00000595937.5; ENSP00000470144.1; ENSG00000182264.9 [Q8IYV9-2] |
GeneIDi | 284359 |
KEGGi | hsa:284359 |
MANE-Selecti | ENST00000332955.7; ENSP00000327786.2; NM_182575.3; NP_872381.2 |
UCSCi | uc002pkj.3, human [Q8IYV9-1] |
Organism-specific databases
CTDi | 284359 |
DisGeNETi | 284359 |
GeneCardsi | IZUMO1 |
HGNCi | HGNC:28539, IZUMO1 |
HPAi | ENSG00000182264, Tissue enriched (testis) |
MIMi | 609278, gene |
neXtProti | NX_Q8IYV9 |
OpenTargetsi | ENSG00000182264 |
PharmGKBi | PA142671647 |
VEuPathDBi | HostDB:ENSG00000182264 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SFD8, Eukaryota |
GeneTreei | ENSGT00390000015014 |
HOGENOMi | CLU_044481_0_0_1 |
InParanoidi | Q8IYV9 |
OMAi | PATIINF |
OrthoDBi | 813463at2759 |
PhylomeDBi | Q8IYV9 |
TreeFami | TF338356 |
Enzyme and pathway databases
PathwayCommonsi | Q8IYV9 |
Reactomei | R-HSA-1300645, Acrosome Reaction and Sperm:Oocyte Membrane Binding |
SignaLinki | Q8IYV9 |
Miscellaneous databases
BioGRID-ORCSi | 284359, 13 hits in 1069 CRISPR screens |
ChiTaRSi | IZUMO1, human |
GenomeRNAii | 284359 |
Pharosi | Q8IYV9, Tbio |
PROi | PR:Q8IYV9 |
RNActi | Q8IYV9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182264, Expressed in testis and 106 other tissues |
ExpressionAtlasi | Q8IYV9, baseline and differential |
Genevisiblei | Q8IYV9, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR029389, IZUMO IPR032699, Izumo-Ig IPR032700, IZUMO1 |
PANTHERi | PTHR35540, PTHR35540, 1 hit |
Pfami | View protein in Pfam PF15005, IZUMO, 1 hit PF16706, Izumo-Ig, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IZUM1_HUMAN | |
Accessioni | Q8IYV9Primary (citable) accession number: Q8IYV9 Secondary accession number(s): Q6Q8P6, Q6Q8P7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2006 |
Last sequence update: | May 18, 2010 | |
Last modified: | May 25, 2022 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families