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Protein

E3 ubiquitin-protein ligase TRIM22

Gene

TRIM22

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interferon-induced antiviral protein involved in cell innate immunity. The antiviral activity could in part be mediated by TRIM22-dependent ubiquitination of viral proteins. Plays a role in restricting the replication of HIV-1, encephalomyocarditis virus (EMCV) and hepatitis B virus (HBV). Acts as a transcriptional repressor of HBV core promoter. May have E3 ubiquitin-protein ligase activity.4 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 60RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri92 – 133B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • DNA-binding transcription factor activity Source: ProtInc
  • protein binding, bridging Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • transcription corepressor activity Source: ProtInc
  • transferase activity Source: UniProtKB-KW
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionRepressor, Transferase
Biological processAntiviral defense, Host-virus interaction, Transcription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.B10 2681
6.3.2.19 2681
ReactomeiR-HSA-877300 Interferon gamma signaling
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM22 (EC:2.3.2.27)
Alternative name(s):
50 kDa-stimulated trans-acting factor
RING finger protein 94
RING-type E3 ubiquitin transferase TRIM22Curated
Staf-50
Tripartite motif-containing protein 22
Gene namesi
Name:TRIM22
Synonyms:RNF94, STAF50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000132274.15
HGNCiHGNC:16379 TRIM22
MIMi606559 gene
neXtProtiNX_Q8IYM9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15C → A: Loss of E3 ubiquitin-protein ligase activity, reduces auto-ubiquitination and not affect nuclear bodies formation. Loss of antiviral activity; when associated with A-18. 3 Publications1
Mutagenesisi18C → A: Loss of antiviral activity and not affect nuclear bodies formation; when associated with A-15. 2 Publications1
Mutagenesisi493 – 494VC → AA: Reduces formation of regular nuclear bodies. 1 Publication2

Organism-specific databases

DisGeNETi10346
OpenTargetsiENSG00000132274
PharmGKBiPA38129

Polymorphism and mutation databases

BioMutaiTRIM22
DMDMi47606181

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562321 – 498E3 ubiquitin-protein ligase TRIM22Add BLAST498

Post-translational modificationi

Auto-ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ8IYM9
MaxQBiQ8IYM9
PaxDbiQ8IYM9
PeptideAtlasiQ8IYM9
PRIDEiQ8IYM9
ProteomicsDBi71205
71206 [Q8IYM9-2]

PTM databases

iPTMnetiQ8IYM9
PhosphoSitePlusiQ8IYM9

Expressioni

Tissue specificityi

Strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary. Expressed at basal levels in other tissues.1 Publication

Inductioni

By interferons alpha and beta. Up-regulated by p53/TP53. Dramatically induced by progesterone in MDA-MB-231-derived ABC28 cells and T47D cells.4 Publications

Gene expression databases

BgeeiENSG00000132274 Expressed in 222 organ(s), highest expression level in blood
CleanExiHS_TRIM22
ExpressionAtlasiQ8IYM9 baseline and differential
GenevisibleiQ8IYM9 HS

Organism-specific databases

HPAiHPA003307
HPA003575

Interactioni

Subunit structurei

Homotrimer (PubMed:17156811).1 Publication
(Microbial infection) Interacts with HIV-1 Gag polyprotein; this interaction seems to reduce gag production or virus budding.1 Publication
(Microbial infection) Interacts with EMCV protease 3C; this interaction leads to viral protease ubiquitination.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115628, 16 interactors
IntActiQ8IYM9, 12 interactors
STRINGi9606.ENSP00000369299

Structurei

3D structure databases

ProteinModelPortaliQ8IYM9
SMRiQ8IYM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini283 – 498B30.2/SPRYPROSITE-ProRule annotationAdd BLAST216

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili132 – 248Sequence analysisAdd BLAST117

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi257 – 275Nuclear localization signalSequence analysisAdd BLAST19

Domaini

The C-terminal SPRY domain is required for the transcriptional suppressor activity, probably by mediating correct nuclear localization. Residues 491-494 are essential for nuclear localization and nuclear bodies formation.
The RING domain is essential for antiviral activity and for TRIM22 nuclear bodies (NB) formation but is not necessary for nuclear localization.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 60RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri92 – 133B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITG7 Eukaryota
ENOG410XWDC LUCA
GeneTreeiENSGT00760000118893
HOGENOMiHOG000234134
HOVERGENiHBG001357
InParanoidiQ8IYM9
KOiK11999
OMAiWVCELSQ
OrthoDBiEOG091G04O8
PhylomeDBiQ8IYM9
TreeFamiTF342569

Family and domain databases

CDDicd00021 BBOX, 1 hit
cd15824 SPRY_PRY_TRIM22, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR035827 PRY/SPRY_TRIM22
IPR003877 SPRY_dom
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IYM9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDFSVKVDIE KEVTCPICLE LLTEPLSLDC GHSFCQACIT AKIKESVIIS
60 70 80 90 100
RGESSCPVCQ TRFQPGNLRP NRHLANIVER VKEVKMSPQE GQKRDVCEHH
110 120 130 140 150
GKKLQIFCKE DGKVICWVCE LSQEHQGHQT FRINEVVKEC QEKLQVALQR
160 170 180 190 200
LIKEDQEAEK LEDDIRQERT AWKNYIQIER QKILKGFNEM RVILDNEEQR
210 220 230 240 250
ELQKLEEGEV NVLDNLAAAT DQLVQQRQDA STLISDLQRR LRGSSVEMLQ
260 270 280 290 300
DVIDVMKRSE SWTLKKPKSV SKKLKSVFRV PDLSGMLQVL KELTDVQYYW
310 320 330 340 350
VDVMLNPGSA TSNVAISVDQ RQVKTVRTCT FKNSNPCDFS AFGVFGCQYF
360 370 380 390 400
SSGKYYWEVD VSGKIAWILG VHSKISSLNK RKSSGFAFDP SVNYSKVYSR
410 420 430 440 450
YRPQYGYWVI GLQNTCEYNA FEDSSSSDPK VLTLFMAVPP CRIGVFLDYE
460 470 480 490
AGIVSFFNVT NHGALIYKFS GCRFSRPAYP YFNPWNCLVP MTVCPPSS
Note: No experimental confirmation available.
Length:498
Mass (Da):56,947
Last modified:March 1, 2003 - v1
Checksum:iAFADB968DE76F7DD
GO
Isoform 2 (identifier: Q8IYM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-177: Missing.

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):56,429
Checksum:i22A7DA4B4B697621
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J060C9J060_HUMAN
E3 ubiquitin-protein ligase TRIM22
TRIM22
203Annotation score:
C9JIU5C9JIU5_HUMAN
E3 ubiquitin-protein ligase TRIM22
TRIM22
227Annotation score:
H7BZD3H7BZD3_HUMAN
E3 ubiquitin-protein ligase TRIM22
TRIM22
143Annotation score:
C9JWC5C9JWC5_HUMAN
E3 ubiquitin-protein ligase TRIM22
TRIM22
305Annotation score:
H7C2K0H7C2K0_HUMAN
E3 ubiquitin-protein ligase TRIM22
TRIM22
63Annotation score:
H7C262H7C262_HUMAN
E3 ubiquitin-protein ligase TRIM22
TRIM22
38Annotation score:
H7C376H7C376_HUMAN
E3 ubiquitin-protein ligase TRIM22
TRIM22
37Annotation score:

Sequence cautioni

The sequence CAA57684 differs from that shown. Reason: Frameshift at position 439.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464A → R in CAA57684 (PubMed:7797467).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052134155D → N1 PublicationCorresponds to variant dbSNP:rs7935564Ensembl.1
Natural variantiVAR_052135232T → A. Corresponds to variant dbSNP:rs2291843Ensembl.1
Natural variantiVAR_052136242R → T1 PublicationCorresponds to variant dbSNP:rs1063303Ensembl.1
Natural variantiVAR_052137321R → K. Corresponds to variant dbSNP:rs12364019Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012060174 – 177Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82200 mRNA Translation: CAA57684.1 Frameshift.
BC022281 mRNA Translation: AAH22281.1
BC035582 mRNA Translation: AAH35582.1
CCDSiCCDS41612.1 [Q8IYM9-1]
PIRiA57041
RefSeqiNP_001186502.1, NM_001199573.1 [Q8IYM9-2]
NP_006065.2, NM_006074.4 [Q8IYM9-1]
UniGeneiHs.501778
Hs.684559
Hs.733231

Genome annotation databases

EnsembliENST00000379965; ENSP00000369299; ENSG00000132274 [Q8IYM9-1]
GeneIDi10346
KEGGihsa:10346
UCSCiuc001mbr.4 human [Q8IYM9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82200 mRNA Translation: CAA57684.1 Frameshift.
BC022281 mRNA Translation: AAH22281.1
BC035582 mRNA Translation: AAH35582.1
CCDSiCCDS41612.1 [Q8IYM9-1]
PIRiA57041
RefSeqiNP_001186502.1, NM_001199573.1 [Q8IYM9-2]
NP_006065.2, NM_006074.4 [Q8IYM9-1]
UniGeneiHs.501778
Hs.684559
Hs.733231

3D structure databases

ProteinModelPortaliQ8IYM9
SMRiQ8IYM9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115628, 16 interactors
IntActiQ8IYM9, 12 interactors
STRINGi9606.ENSP00000369299

PTM databases

iPTMnetiQ8IYM9
PhosphoSitePlusiQ8IYM9

Polymorphism and mutation databases

BioMutaiTRIM22
DMDMi47606181

Proteomic databases

EPDiQ8IYM9
MaxQBiQ8IYM9
PaxDbiQ8IYM9
PeptideAtlasiQ8IYM9
PRIDEiQ8IYM9
ProteomicsDBi71205
71206 [Q8IYM9-2]

Protocols and materials databases

DNASUi10346
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379965; ENSP00000369299; ENSG00000132274 [Q8IYM9-1]
GeneIDi10346
KEGGihsa:10346
UCSCiuc001mbr.4 human [Q8IYM9-1]

Organism-specific databases

CTDi10346
DisGeNETi10346
EuPathDBiHostDB:ENSG00000132274.15
GeneCardsiTRIM22
HGNCiHGNC:16379 TRIM22
HPAiHPA003307
HPA003575
MIMi606559 gene
neXtProtiNX_Q8IYM9
OpenTargetsiENSG00000132274
PharmGKBiPA38129
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITG7 Eukaryota
ENOG410XWDC LUCA
GeneTreeiENSGT00760000118893
HOGENOMiHOG000234134
HOVERGENiHBG001357
InParanoidiQ8IYM9
KOiK11999
OMAiWVCELSQ
OrthoDBiEOG091G04O8
PhylomeDBiQ8IYM9
TreeFamiTF342569

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B10 2681
6.3.2.19 2681
ReactomeiR-HSA-877300 Interferon gamma signaling

Miscellaneous databases

GeneWikiiTRIM22
GenomeRNAii10346
PROiPR:Q8IYM9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132274 Expressed in 222 organ(s), highest expression level in blood
CleanExiHS_TRIM22
ExpressionAtlasiQ8IYM9 baseline and differential
GenevisibleiQ8IYM9 HS

Family and domain databases

CDDicd00021 BBOX, 1 hit
cd15824 SPRY_PRY_TRIM22, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR035827 PRY/SPRY_TRIM22
IPR003877 SPRY_dom
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRI22_HUMAN
AccessioniPrimary (citable) accession number: Q8IYM9
Secondary accession number(s): Q05CQ0, Q15521
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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