UniProtKB - Q8IYL9 (PSYR_HUMAN)
Protein
Psychosine receptor
Gene
GPR65
Organism
Homo sapiens (Human)
Status
Functioni
Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421). Plays a role in immune response by maintaining lysosome function and supporting phagocytosis-mediated intracellular bacteria clearance (PubMed:27287411). May have a role in activation-induced cell death or differentiation of T-cells (By similarity).By similarity2 Publications
GO - Molecular functioni
- G protein-coupled receptor activity Source: BHF-UCL
GO - Biological processi
- actin cytoskeleton reorganization Source: BHF-UCL
- activation of GTPase activity Source: BHF-UCL
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
- apoptotic process Source: ProtInc
- G protein-coupled receptor signaling pathway Source: BHF-UCL
- immune response Source: ProtInc
- multicellular organism development Source: ProtInc
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway Source: GO_Central
- positive regulation of Rho protein signal transduction Source: GO_Central
- positive regulation of stress fiber assembly Source: BHF-UCL
- response to acidic pH Source: BHF-UCL
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Apoptosis |
Enzyme and pathway databases
PathwayCommonsi | Q8IYL9 |
Reactomei | R-HSA-373076, Class A/1 (Rhodopsin-like receptors) R-HSA-416476, G alpha (q) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Psychosine receptorAlternative name(s): G-protein coupled receptor 65 T-cell death-associated gene 8 protein |
Gene namesi | Name:GPR65 Synonyms:TDAG8 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000140030.5 |
HGNCi | HGNC:4517, GPR65 |
MIMi | 604620, gene |
neXtProti | NX_Q8IYL9 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 16 – 36 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 37 – 50 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 51 – 71 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 72 – 89 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 90 – 110 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 111 – 129 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 130 – 150 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 151 – 180 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 181 – 201 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 202 – 227 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 228 – 248 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 249 – 273 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 274 – 294 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 295 – 337 | CytoplasmicSequence analysisAdd BLAST | 43 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 8477 |
OpenTargetsi | ENSG00000140030 |
PharmGKBi | PA28909 |
Miscellaneous databases
Pharosi | Q8IYL9, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3714081 |
Polymorphism and mutation databases
BioMutai | GPR65 |
DMDMi | 37537957 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070087 | 1 – 337 | Psychosine receptorAdd BLAST | 337 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 2 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 79 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 87 ↔ 170 | PROSITE-ProRule annotation | ||
Glycosylationi | 166 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q8IYL9 |
PeptideAtlasi | Q8IYL9 |
PRIDEi | Q8IYL9 |
ProteomicsDBi | 71200 |
PTM databases
GlyGeni | Q8IYL9, 3 sites |
iPTMneti | Q8IYL9 |
PhosphoSitePlusi | Q8IYL9 |
Expressioni
Tissue specificityi
Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes.2 Publications
Gene expression databases
Bgeei | ENSG00000140030, Expressed in blood and 144 other tissues |
ExpressionAtlasi | Q8IYL9, baseline and differential |
Genevisiblei | Q8IYL9, HS |
Organism-specific databases
HPAi | ENSG00000140030, Tissue enhanced (blood, lymphoid tissue) |
Interactioni
Protein-protein interaction databases
IntActi | Q8IYL9, 1 interactor |
STRINGi | 9606.ENSP00000267549 |
Chemistry databases
BindingDBi | Q8IYL9 |
Miscellaneous databases
RNActi | Q8IYL9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222366 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q8IYL9 |
OMAi | TGRSDMW |
OrthoDBi | 987208at2759 |
PhylomeDBi | Q8IYL9 |
TreeFami | TF331803 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR005464, Psych_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01649, PSYCHOSINER |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8IYL9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNSTCIEEQH DLDHYLFPIV YIFVIIVSIP ANIGSLCVSF LQAKKESELG
60 70 80 90 100
IYLFSLSLSD LLYALTLPLW IDYTWNKDNW TFSPALCKGS AFLMYMNFYS
110 120 130 140 150
STAFLTCIAV DRYLAVVYPL KFFFLRTRRF ALMVSLSIWI LETIFNAVML
160 170 180 190 200
WEDETVVEYC DAEKSNFTLC YDKYPLEKWQ INLNLFRTCT GYAIPLVTIL
210 220 230 240 250
ICNRKVYQAV RHNKATENKE KKRIIKLLVS ITVTFVLCFT PFHVMLLIRC
260 270 280 290 300
ILEHAVNFED HSNSGKRTYT MYRITVALTS LNCVADPILY CFVTETGRYD
310 320 330
MWNILKFCTG RCNTSQRQRK RILSVSTKDT MELEVLE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 | A → P in AAC31794 (PubMed:9655242).Curated | 1 | |
Sequence conflicti | 97 | N → K in AAC31794 (PubMed:9655242).Curated | 1 | |
Sequence conflicti | 130 | F → I in AAC31794 (PubMed:9655242).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_022064 | 231 | I → L Polymorphism; increased lysosomal pH; increased lipid droplets accumulation. Corresponds to variant dbSNP:rs3742704Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95218 Genomic DNA Translation: AAC31794.1 AL157955 Genomic DNA No translation available. BC035633 mRNA Translation: AAH35633.1 BC071715 mRNA Translation: AAH71715.1 |
CCDSi | CCDS9879.1 |
RefSeqi | NP_003599.2, NM_003608.3 |
Genome annotation databases
Ensembli | ENST00000267549; ENSP00000267549; ENSG00000140030 |
GeneIDi | 8477 |
KEGGi | hsa:8477 |
UCSCi | uc001xvv.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95218 Genomic DNA Translation: AAC31794.1 AL157955 Genomic DNA No translation available. BC035633 mRNA Translation: AAH35633.1 BC071715 mRNA Translation: AAH71715.1 |
CCDSi | CCDS9879.1 |
RefSeqi | NP_003599.2, NM_003608.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q8IYL9, 1 interactor |
STRINGi | 9606.ENSP00000267549 |
Chemistry databases
BindingDBi | Q8IYL9 |
ChEMBLi | CHEMBL3714081 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q8IYL9, 3 sites |
iPTMneti | Q8IYL9 |
PhosphoSitePlusi | Q8IYL9 |
Polymorphism and mutation databases
BioMutai | GPR65 |
DMDMi | 37537957 |
Proteomic databases
PaxDbi | Q8IYL9 |
PeptideAtlasi | Q8IYL9 |
PRIDEi | Q8IYL9 |
ProteomicsDBi | 71200 |
Protocols and materials databases
Antibodypediai | 13320, 216 antibodies |
DNASUi | 8477 |
Genome annotation databases
Ensembli | ENST00000267549; ENSP00000267549; ENSG00000140030 |
GeneIDi | 8477 |
KEGGi | hsa:8477 |
UCSCi | uc001xvv.3, human |
Organism-specific databases
CTDi | 8477 |
DisGeNETi | 8477 |
EuPathDBi | HostDB:ENSG00000140030.5 |
GeneCardsi | GPR65 |
HGNCi | HGNC:4517, GPR65 |
HPAi | ENSG00000140030, Tissue enhanced (blood, lymphoid tissue) |
MIMi | 604620, gene |
neXtProti | NX_Q8IYL9 |
OpenTargetsi | ENSG00000140030 |
PharmGKBi | PA28909 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222366 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q8IYL9 |
OMAi | TGRSDMW |
OrthoDBi | 987208at2759 |
PhylomeDBi | Q8IYL9 |
TreeFami | TF331803 |
Enzyme and pathway databases
PathwayCommonsi | Q8IYL9 |
Reactomei | R-HSA-373076, Class A/1 (Rhodopsin-like receptors) R-HSA-416476, G alpha (q) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 8477, 6 hits in 854 CRISPR screens |
GeneWikii | GPR65 |
GenomeRNAii | 8477 |
Pharosi | Q8IYL9, Tbio |
PROi | PR:Q8IYL9 |
RNActi | Q8IYL9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000140030, Expressed in blood and 144 other tissues |
ExpressionAtlasi | Q8IYL9, baseline and differential |
Genevisiblei | Q8IYL9, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR005464, Psych_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01649, PSYCHOSINER |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSYR_HUMAN | |
Accessioni | Q8IYL9Primary (citable) accession number: Q8IYL9 Secondary accession number(s): O75819 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries