UniProtKB - Q8IY81 (SPB1_HUMAN)
Protein
pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
Gene
FTSJ3
Organism
Homo sapiens (Human)
Status
Functioni
RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.UniRule annotation1 Publication
(Microbial infection) In case of infection by HIV-1 virus, recruited to HIV-1 RNA and catalyzes 2'-O-methylation of the viral genome, allowing HIV-1 virus to escape the innate immune system (PubMed:30626973). RNA 2'-O-methylation provides a molecular signature for discrimination of self from non-self and is used by HIV-1 to evade innate immune recognition by IFIH1/MDA5 (PubMed:30626973). Mediates methylation of internal residues of HIV-1 RNA, with a strong preference for adenosine (PubMed:30626973). Recruited to HIV-1 RNA via interaction with TARBP2/TRBP (PubMed:30626973).1 Publication
Catalytic activityi
- a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in rRNA + H+ + S-adenosyl-L-homocysteineUniRule annotation
- a ribonucleotide in RNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in RNA + H+ + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 56 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 58 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 76 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 92 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 117 | S-adenosyl-L-methionineUniRule annotation1 Publication | 1 | |
Active sitei | 157 | Proton acceptorUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- RNA 2'-O-methyltransferase activity Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA methyltransferase activity Source: GO_Central
- rRNA (guanine) methyltransferase activity Source: GO_Central
- rRNA (uridine-2'-O-)-methyltransferase activity Source: GO_Central
GO - Biological processi
- enzyme-directed rRNA 2'-O-methylation Source: UniProtKB-UniRule
- maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- RNA methylation Source: UniProtKB
- rRNA methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Ribosome biogenesis, rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | Q8IY81 |
Reactomei | R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3UniRule annotation (EC:2.1.1.-UniRule annotation1 Publication)Alternative name(s): Protein ftsJ homolog 3UniRule annotation Putative rRNA methyltransferase 3UniRule annotation |
Gene namesi | Name:FTSJ3UniRule annotation ORF Names:SB92 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000108592.16 |
HGNCi | HGNC:17136, FTSJ3 |
MIMi | 618411, gene |
neXtProti | NX_Q8IY81 |
Subcellular locationi
Nucleus
- nucleolus UniRule annotation2 Publications
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
Other locations
- preribosome, large subunit precursor Source: GO_Central
- preribosome, small subunit precursor Source: UniProtKB-UniRule
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | K → A: Abolishes RNA 2'-O-methyltransferase activity; when associated with A-117 and A-157. 1 Publication | 1 | |
Mutagenesisi | 117 | D → A: Abolishes RNA 2'-O-methyltransferase activity; when associated with A-31 and A-157. 1 Publication | 1 | |
Mutagenesisi | 157 | K → A: Abolishes RNA 2'-O-methyltransferase activity; when associated with A-31 and A-117. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 117246 |
OpenTargetsi | ENSG00000108592 |
PharmGKBi | PA28419 |
Miscellaneous databases
Pharosi | Q8IY81, Tbio |
Polymorphism and mutation databases
BioMutai | FTSJ3 |
DMDMi | 296452883 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000155577 | 1 – 847 | pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3Add BLAST | 847 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 333 | PhosphoserineCombined sources | 1 | |
Modified residuei | 335 | PhosphoserineCombined sources | 1 | |
Modified residuei | 336 | PhosphoserineCombined sources | 1 | |
Modified residuei | 347 | PhosphoserineCombined sources | 1 | |
Modified residuei | 356 | PhosphoserineCombined sources | 1 | |
Cross-linki | 357 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 392 | CitrullineBy similarity | 1 | |
Modified residuei | 549 | PhosphoserineCombined sources | 1 | |
Modified residuei | 573 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 579 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 584 | PhosphoserineCombined sources | 1 | |
Cross-linki | 643 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 644 | PhosphoserineCombined sources | 1 | |
Cross-linki | 659 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 676 | PhosphoserineCombined sources | 1 | |
Cross-linki | 678 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 688 | PhosphoserineCombined sources | 1 | |
Cross-linki | 710 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 783 | CitrullineBy similarity | 1 |
Post-translational modificationi
Citrullinated by PADI4.By similarity
Keywords - PTMi
Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8IY81 |
jPOSTi | Q8IY81 |
MassIVEi | Q8IY81 |
MaxQBi | Q8IY81 |
PaxDbi | Q8IY81 |
PeptideAtlasi | Q8IY81 |
PRIDEi | Q8IY81 |
ProteomicsDBi | 71121 |
2D gel databases
SWISS-2DPAGEi | Q8IY81 |
PTM databases
iPTMneti | Q8IY81 |
PhosphoSitePlusi | Q8IY81 |
SwissPalmi | Q8IY81 |
Expressioni
Gene expression databases
Bgeei | ENSG00000108592, Expressed in sural nerve and 215 other tissues |
ExpressionAtlasi | Q8IY81, baseline and differential |
Genevisiblei | Q8IY81, HS |
Organism-specific databases
HPAi | ENSG00000108592, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with NIP7.
UniRule annotation1 PublicationBinary interactionsi
Hide detailsQ8IY81
With | #Exp. | IntAct |
---|---|---|
EIF2AK2 [P19525] | 2 | EBI-744088,EBI-640775 |
H1-4 [P10412] | 2 | EBI-744088,EBI-358163 |
OAS3 [Q9Y6K5] | 2 | EBI-744088,EBI-6115729 |
SSX2B - isoform 2 [Q16385-2] | 3 | EBI-744088,EBI-17564583 |
Protein-protein interaction databases
BioGRIDi | 125581, 191 interactors |
IntActi | Q8IY81, 83 interactors |
MINTi | Q8IY81 |
STRINGi | 9606.ENSP00000396673 |
Miscellaneous databases
RNActi | Q8IY81, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 355 – 407 | UniRule annotationAdd BLAST | 53 | |
Coiled coili | 739 – 777 | UniRule annotationAdd BLAST | 39 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.UniRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1098, Eukaryota |
GeneTreei | ENSGT00550000075004 |
HOGENOMi | CLU_009422_8_1_1 |
InParanoidi | Q8IY81 |
OMAi | EILECCK |
OrthoDBi | 1362679at2759 |
PhylomeDBi | Q8IY81 |
TreeFami | TF106102 |
Family and domain databases
HAMAPi | MF_01547, RNA_methyltr_E, 1 hit MF_03163, RNA_methyltr_E_SPB1, 1 hit |
InterProi | View protein in InterPro IPR015507, rRNA-MeTfrase_E IPR012920, rRNA_MeTfrase_Spb1_C IPR024576, rRNA_MeTfrase_Spb1_DUF3381 IPR002877, rRNA_MeTrfase_FtsJ_dom IPR029063, SAM-dependent_MTases IPR028589, Spb1-like |
Pfami | View protein in Pfam PF11861, DUF3381, 1 hit PF01728, FtsJ, 1 hit PF07780, Spb1_C, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All
Q8IY81-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKKGKVGKS RRDKFYHLAK ETGYRSRSAF KLIQLNRRFQ FLQKARALLD
60 70 80 90 100
LCAAPGGWLQ VAAKFMPVSS LIVGVDLVPI KPLPNVVTLQ QDITTERCRQ
110 120 130 140 150
ALRKELKTWK VDVVLNDGAP NVGASWVHDA YSQAHLTLMA LRLACDFLAR
160 170 180 190 200
GGSFITKVFR SRDYQPLLWI FQQLFRRVQA TKPQASRHES AEIFVVCQGF
210 220 230 240 250
LAPDKVDSKF FDPKFAFKEV EVQAKTVTEL VTKKKPKAEG YAEGDLTLYH
260 270 280 290 300
RTSVTDFLRA ANPVDFLSKA SEIMVDDEEL AQHPATTEDI RVCCQDIRVL
310 320 330 340 350
GRKELRSLLN WRTKLRRYVA KKLKEQAKAL DISLSSGEED EGDEEDSTAG
360 370 380 390 400
TTKQPSKEEE EEEEEEQLNQ TLAEMKAQEV AELKRKKKKL LREQRKQRER
410 420 430 440 450
VELKMDLPGV SIADEGETGM FSLSTIRGHQ LLEEVTQGDM SAADTFLSDL
460 470 480 490 500
PRDDIYVSDV EDDGDDTSLD SDLDPEELAG VRGHQGLRDQ KRMRLTEVQD
510 520 530 540 550
DKEEEEEENP LLVPLEEKAV LQEEQANLWF SKGSFAGIED DADEALEISQ
560 570 580 590 600
AQLLFENRRK GRQQQQKQQL PQTPPSCLKT EIMSPLYQDE APKGTEASSG
610 620 630 640 650
TEAATGLEGE EKDGISDSDS STSSEEEESW EPLRGKKRSR GPKSDDDGFE
660 670 680 690 700
IVPIEDPAKH RILDPEGLAL GAVIASSKKA KRDLIDNSFN RYTFNEDEGE
710 720 730 740 750
LPEWFVQEEK QHRIRQLPVG KKEVEHYRKR WREINARPIK KVAEAKARKK
760 770 780 790 800
RRMLKRLEQT RKKAEAVVNT VDISEREKVA QLRSLYKKAG LGKEKRHVTY
810 820 830 840
VVAKKGVGRK VRRPAGVRGH FKVVDSRMKK DQRAQQRKEQ KKKHKRK
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KS36 | J3KS36_HUMAN | pre-rRNA 2'-O-ribose RNA methyltran... | FTSJ3 | 151 | Annotation score: | ||
J3QSE2 | J3QSE2_HUMAN | pre-rRNA 2'-O-ribose RNA methyltran... | FTSJ3 | 115 | Annotation score: | ||
J3KS74 | J3KS74_HUMAN | pre-rRNA 2'-O-ribose RNA methyltran... | FTSJ3 | 67 | Annotation score: | ||
J3QKT6 | J3QKT6_HUMAN | pre-rRNA 2'-O-ribose RNA methyltran... | FTSJ3 | 19 | Annotation score: | ||
J3QRS5 | J3QRS5_HUMAN | pre-rRNA 2'-O-ribose RNA methyltran... | FTSJ3 | 33 | Annotation score: | ||
J3KRY4 | J3KRY4_HUMAN | pre-rRNA 2'-O-ribose RNA methyltran... | FTSJ3 | 29 | Annotation score: | ||
J3QR05 | J3QR05_HUMAN | pre-rRNA 2'-O-ribose RNA methyltran... | FTSJ3 | 40 | Annotation score: |
Sequence cautioni
The sequence BAA90924 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 278 | E → I in AAL56015 (Ref. 1) Curated | 1 | |
Sequence conflicti | 278 | E → I in BAA90924 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_023284 | 91 | Q → E3 PublicationsCorresponds to variant dbSNP:rs2584625Ensembl. | 1 | |
Natural variantiVAR_023285 | 424 | S → C4 PublicationsCorresponds to variant dbSNP:rs2727288Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327355 mRNA Translation: AAL56015.1 AK000069 mRNA Translation: BAA90924.1 Different initiation. AK315010 mRNA Translation: BAG37502.1 AC015651 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94273.1 CH471109 Genomic DNA Translation: EAW94274.1 CH471109 Genomic DNA Translation: EAW94275.1 BC000131 mRNA Translation: AAH00131.2 BC036710 mRNA Translation: AAH36710.1 AL834482 mRNA Translation: CAD39141.1 |
CCDSi | CCDS11644.1 |
RefSeqi | NP_060117.3, NM_017647.3 |
Genome annotation databases
Ensembli | ENST00000427159; ENSP00000396673; ENSG00000108592 |
GeneIDi | 117246 |
KEGGi | hsa:117246 |
UCSCi | uc002jca.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327355 mRNA Translation: AAL56015.1 AK000069 mRNA Translation: BAA90924.1 Different initiation. AK315010 mRNA Translation: BAG37502.1 AC015651 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94273.1 CH471109 Genomic DNA Translation: EAW94274.1 CH471109 Genomic DNA Translation: EAW94275.1 BC000131 mRNA Translation: AAH00131.2 BC036710 mRNA Translation: AAH36710.1 AL834482 mRNA Translation: CAD39141.1 |
CCDSi | CCDS11644.1 |
RefSeqi | NP_060117.3, NM_017647.3 |
3D structure databases
SMRi | Q8IY81 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125581, 191 interactors |
IntActi | Q8IY81, 83 interactors |
MINTi | Q8IY81 |
STRINGi | 9606.ENSP00000396673 |
PTM databases
iPTMneti | Q8IY81 |
PhosphoSitePlusi | Q8IY81 |
SwissPalmi | Q8IY81 |
Polymorphism and mutation databases
BioMutai | FTSJ3 |
DMDMi | 296452883 |
2D gel databases
SWISS-2DPAGEi | Q8IY81 |
Proteomic databases
EPDi | Q8IY81 |
jPOSTi | Q8IY81 |
MassIVEi | Q8IY81 |
MaxQBi | Q8IY81 |
PaxDbi | Q8IY81 |
PeptideAtlasi | Q8IY81 |
PRIDEi | Q8IY81 |
ProteomicsDBi | 71121 |
Protocols and materials databases
Antibodypediai | 31352, 108 antibodies |
DNASUi | 117246 |
Genome annotation databases
Ensembli | ENST00000427159; ENSP00000396673; ENSG00000108592 |
GeneIDi | 117246 |
KEGGi | hsa:117246 |
UCSCi | uc002jca.3, human |
Organism-specific databases
CTDi | 117246 |
DisGeNETi | 117246 |
EuPathDBi | HostDB:ENSG00000108592.16 |
GeneCardsi | FTSJ3 |
HGNCi | HGNC:17136, FTSJ3 |
HPAi | ENSG00000108592, Low tissue specificity |
MIMi | 618411, gene |
neXtProti | NX_Q8IY81 |
OpenTargetsi | ENSG00000108592 |
PharmGKBi | PA28419 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1098, Eukaryota |
GeneTreei | ENSGT00550000075004 |
HOGENOMi | CLU_009422_8_1_1 |
InParanoidi | Q8IY81 |
OMAi | EILECCK |
OrthoDBi | 1362679at2759 |
PhylomeDBi | Q8IY81 |
TreeFami | TF106102 |
Enzyme and pathway databases
PathwayCommonsi | Q8IY81 |
Reactomei | R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 117246, 483 hits in 846 CRISPR screens |
ChiTaRSi | FTSJ3, human |
GeneWikii | FTSJ3 |
GenomeRNAii | 117246 |
Pharosi | Q8IY81, Tbio |
PROi | PR:Q8IY81 |
RNActi | Q8IY81, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000108592, Expressed in sural nerve and 215 other tissues |
ExpressionAtlasi | Q8IY81, baseline and differential |
Genevisiblei | Q8IY81, HS |
Family and domain databases
HAMAPi | MF_01547, RNA_methyltr_E, 1 hit MF_03163, RNA_methyltr_E_SPB1, 1 hit |
InterProi | View protein in InterPro IPR015507, rRNA-MeTfrase_E IPR012920, rRNA_MeTfrase_Spb1_C IPR024576, rRNA_MeTfrase_Spb1_DUF3381 IPR002877, rRNA_MeTrfase_FtsJ_dom IPR029063, SAM-dependent_MTases IPR028589, Spb1-like |
Pfami | View protein in Pfam PF11861, DUF3381, 1 hit PF01728, FtsJ, 1 hit PF07780, Spb1_C, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPB1_HUMAN | |
Accessioni | Q8IY81Primary (citable) accession number: Q8IY81 Secondary accession number(s): B2RCA5 Q9NXT6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | May 18, 2010 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations