UniProtKB - Q8IXN7 (RIMKA_HUMAN)
Protein
N-acetylaspartylglutamate synthase A
Gene
RIMKLA
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the synthesis of N-acetyl-L-aspartyl-L-glutamate (NAAG) and N-acetyl-L-aspartyl-L-glutamyl-L-glutamate.By similarity
Miscellaneous
N-acetyl-L-aspartyl-L-glutamate (NAAG) is the most abundant dipeptide present in vertebrate central nervous system (CNS).By similarity
Catalytic activityi
- ATP + L-glutamate + N-acetyl-L-aspartate = ADP + H+ + N-acetyl-L-aspartyl-L-glutamate + phosphateBy similarityEC:6.3.2.41By similarity
- 2 ATP + 2 L-glutamate + N-acetyl-L-aspartate = 2 ADP + 2 H+ + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate + 2 phosphateBy similarityEC:6.3.2.42By similarity
Cofactori
Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 154 | ATPBy similarity | 1 | |
Binding sitei | 215 | ATPBy similarity | 1 | |
Metal bindingi | 260 | Magnesium or manganese 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 273 | Magnesium or manganese 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 273 | Magnesium or manganese 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 275 | Magnesium or manganese 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 189 – 199 | ATPPROSITE-ProRule annotationAdd BLAST | 11 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ligase activity, forming carbon-nitrogen bonds Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- N-acetyl-L-aspartate-L-glutamate ligase activity Source: UniProtKB
GO - Biological processi
- cellular amino acid biosynthetic process Source: Reactome
- cellular protein modification process Source: InterPro
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8IXN7 |
Reactomei | R-HSA-8964539, Glutamate and glutamine metabolism |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RIMKLA Synonyms:FAM80A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28725, RIMKLA |
MIMi | 618949, gene |
neXtProti | NX_Q8IXN7 |
VEuPathDBi | HostDB:ENSG00000177181.14 |
Pathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000177181 |
PharmGKBi | PA164725339 |
Miscellaneous databases
Pharosi | Q8IXN7, Tdark |
Genetic variation databases
BioMutai | RIMKLA |
DMDMi | 143458650 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000282568 | 1 – 391 | N-acetylaspartylglutamate synthase AAdd BLAST | 391 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 319 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q8IXN7 |
MassIVEi | Q8IXN7 |
PaxDbi | Q8IXN7 |
PeptideAtlasi | Q8IXN7 |
PRIDEi | Q8IXN7 |
ProteomicsDBi | 71034 |
PTM databases
iPTMneti | Q8IXN7 |
PhosphoSitePlusi | Q8IXN7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000177181, Expressed in secondary oocyte and 137 other tissues |
ExpressionAtlasi | Q8IXN7, baseline and differential |
Genevisiblei | Q8IXN7, HS |
Organism-specific databases
HPAi | ENSG00000177181, Tissue enhanced (brain) |
Interactioni
Binary interactionsi
Hide detailsQ8IXN7
With | #Exp. | IntAct |
---|---|---|
FGFR3 [P22607] | 3 | EBI-21890191,EBI-348399 |
GSN [P06396] | 3 | EBI-21890191,EBI-351506 |
HRAS [P01112] | 3 | EBI-21890191,EBI-350145 |
HTT [P42858] | 3 | EBI-21890191,EBI-466029 |
Protein-protein interaction databases
BioGRIDi | 129940, 3 interactors |
IntActi | Q8IXN7, 7 interactors |
STRINGi | 9606.ENSP00000414330 |
Miscellaneous databases
RNActi | Q8IXN7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 115 – 300 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 186 |
Sequence similaritiesi
Belongs to the RimK family.Curated
Phylogenomic databases
eggNOGi | ENOG502QT4M, Eukaryota |
GeneTreei | ENSGT00390000014577 |
HOGENOMi | CLU_054353_3_1_1 |
InParanoidi | Q8IXN7 |
OMAi | TRGHQGK |
OrthoDBi | 753616at2759 |
PhylomeDBi | Q8IXN7 |
TreeFami | TF332035 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013651, ATP-grasp_RimK-type IPR013815, ATP_grasp_subdomain_1 IPR004666, RpS6_RimK/Lys_biosynth_LsyX |
Pfami | View protein in Pfam PF08443, RimK, 1 hit |
TIGRFAMsi | TIGR00768, rimK_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8IXN7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCSQLWFLTD RRIREDYPQV QILRALRQRC SEQDVRFRAV LMDQIAVTIV
60 70 80 90 100
GGHLGLQLNQ KALTTFPDVV LVRVPTPSVQ SDSDITVLRH LEKLGCRLVN
110 120 130 140 150
RPQSILNCIN KFWTFQELAG HGVPMPDTFS YGGHEDFSKM IDEAEPLGYP
160 170 180 190 200
VVVKSTRGHR GKAVFLARDK HHLSDICHLI RHDVPYLFQK YVKESHGKDI
210 220 230 240 250
RVVVVGGQVI GSMLRCSTDG RMQSNCSLGG VGVKCPLTEQ GKQLAIQVSN
260 270 280 290 300
ILGMDFCGID LLIMDDGSFV VCEANANVGF LAFDQACNLD VGGIIADYTM
310 320 330 340 350
SLLPNRQTGK MAVLPGLSSP REKNEPDGCA SAQGVAESVY TINSGSTSSE
360 370 380 390
SEPELGEIRD SSASTMGAPP SMLPEPGYNI NNRIASELKL K
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX1WI25 | X1WI25_HUMAN | N-acetylaspartylglutamate synthase ... | RIMKLA | 97 | Annotation score: |
Sequence cautioni
The sequence AAH39737 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513331 Genomic DNA No translation available. BC039737 mRNA Translation: AAH39737.1 Different initiation. |
CCDSi | CCDS466.2 |
RefSeqi | NP_775913.2, NM_173642.3 |
Genome annotation databases
Ensembli | ENST00000431473; ENSP00000414330; ENSG00000177181 |
GeneIDi | 284716 |
KEGGi | hsa:284716 |
UCSCi | uc001chi.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL513331 Genomic DNA No translation available. BC039737 mRNA Translation: AAH39737.1 Different initiation. |
CCDSi | CCDS466.2 |
RefSeqi | NP_775913.2, NM_173642.3 |
3D structure databases
SMRi | Q8IXN7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 129940, 3 interactors |
IntActi | Q8IXN7, 7 interactors |
STRINGi | 9606.ENSP00000414330 |
PTM databases
iPTMneti | Q8IXN7 |
PhosphoSitePlusi | Q8IXN7 |
Genetic variation databases
BioMutai | RIMKLA |
DMDMi | 143458650 |
Proteomic databases
jPOSTi | Q8IXN7 |
MassIVEi | Q8IXN7 |
PaxDbi | Q8IXN7 |
PeptideAtlasi | Q8IXN7 |
PRIDEi | Q8IXN7 |
ProteomicsDBi | 71034 |
Protocols and materials databases
Antibodypediai | 32199, 89 antibodies |
DNASUi | 284716 |
Genome annotation databases
Ensembli | ENST00000431473; ENSP00000414330; ENSG00000177181 |
GeneIDi | 284716 |
KEGGi | hsa:284716 |
UCSCi | uc001chi.3, human |
Organism-specific databases
CTDi | 284716 |
GeneCardsi | RIMKLA |
HGNCi | HGNC:28725, RIMKLA |
HPAi | ENSG00000177181, Tissue enhanced (brain) |
MIMi | 618949, gene |
neXtProti | NX_Q8IXN7 |
OpenTargetsi | ENSG00000177181 |
PharmGKBi | PA164725339 |
VEuPathDBi | HostDB:ENSG00000177181.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QT4M, Eukaryota |
GeneTreei | ENSGT00390000014577 |
HOGENOMi | CLU_054353_3_1_1 |
InParanoidi | Q8IXN7 |
OMAi | TRGHQGK |
OrthoDBi | 753616at2759 |
PhylomeDBi | Q8IXN7 |
TreeFami | TF332035 |
Enzyme and pathway databases
PathwayCommonsi | Q8IXN7 |
Reactomei | R-HSA-8964539, Glutamate and glutamine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 284716, 10 hits in 876 CRISPR screens |
ChiTaRSi | RIMKLA, human |
GenomeRNAii | 284716 |
Pharosi | Q8IXN7, Tdark |
PROi | PR:Q8IXN7 |
RNActi | Q8IXN7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000177181, Expressed in secondary oocyte and 137 other tissues |
ExpressionAtlasi | Q8IXN7, baseline and differential |
Genevisiblei | Q8IXN7, HS |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013651, ATP-grasp_RimK-type IPR013815, ATP_grasp_subdomain_1 IPR004666, RpS6_RimK/Lys_biosynth_LsyX |
Pfami | View protein in Pfam PF08443, RimK, 1 hit |
TIGRFAMsi | TIGR00768, rimK_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIMKA_HUMAN | |
Accessioni | Q8IXN7Primary (citable) accession number: Q8IXN7 Secondary accession number(s): Q5VUS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | April 3, 2007 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families