Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Negative elongation factor C/D

Gene

NELFCD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner.1 Publication3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor C/D
Short name:
NELF-C/D
Alternative name(s):
TH1-like protein
Gene namesi
Name:NELFCD
Synonyms:NELFD, TH1, TH1L
ORF Names:HSPC130
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

EuPathDBiHostDB:ENSG00000101158.12
HGNCiHGNC:15934 NELFCD
MIMi605297 gene
neXtProtiNX_Q8IXH7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi291R → Q: Reduces RNA binding; when associated with M-315, M-371, M-372, M-374, M-384, M-388, Q-419 and Q-506. 1 Publication1
Mutagenesisi315K → M: Reduces RNA binding; when associated with Q-291, M-371, M-372, M-374, M-384, M-388, Q-419 and Q-506. 1 Publication1
Mutagenesisi371K → M: Reduces RNA binding; when associated with Q-291, M-315, M-372, M-374, M-384, M-388, Q-419 and Q-506. 1 Publication1
Mutagenesisi372K → M: Reduces RNA binding; when associated with Q-291, M-315, M-371, M-374, M-384, M-388, Q-419 and Q-506. 1 Publication1
Mutagenesisi374K → M: Reduces RNA binding; when associated with Q-291, M-315, M-371, M-372, M-384, M-388, Q-419 and Q-506. 1 Publication1
Mutagenesisi384K → M: Reduces RNA binding; when associated with Q-291, M-315, M-371, M-372, M-374, M-388, Q-419 and Q-506. 1 Publication1
Mutagenesisi388K → M: Reduces RNA binding; when associated with Q-291, M-315, M-371, M-372, M-374, M-384, Q-419 and Q-506. 1 Publication1
Mutagenesisi419R → Q: Reduces RNA binding; when associated with Q-291, M-315, M-371, M-372, M-374, M-384, M-388 and Q-506. 1 Publication1
Mutagenesisi506R → Q: Reduces RNA binding; when associated with Q-291, M-315, M-371, M-372, M-374, M-384, M-388 and Q-419. 1 Publication1

Organism-specific databases

DisGeNETi51497
PharmGKBiPA38055

Polymorphism and mutation databases

DMDMi38372376

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000194561 – 590Negative elongation factor C/DAdd BLAST590

Proteomic databases

EPDiQ8IXH7
MaxQBiQ8IXH7
PaxDbiQ8IXH7
PeptideAtlasiQ8IXH7
PRIDEiQ8IXH7
ProteomicsDBi70993
70994 [Q8IXH7-3]
70995 [Q8IXH7-4]

PTM databases

iPTMnetiQ8IXH7
PhosphoSitePlusiQ8IXH7

Expressioni

Tissue specificityi

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas.2 Publications

Gene expression databases

BgeeiENSG00000101158 Expressed in 222 organ(s), highest expression level in adenohypophysis
GenevisibleiQ8IXH7 HS

Organism-specific databases

HPAiHPA050641
HPA066262

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; NELFA and NELFCD form a stable subcomplex that binds primarily through NELFCD to the N-terminus of NELFB. Interacts with ARAF1. Interacts with PCF11.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi119572, 45 interactors
CORUMiQ8IXH7
DIPiDIP-34061N
IntActiQ8IXH7, 31 interactors
MINTiQ8IXH7
STRINGi9606.ENSP00000342300

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8IXH7
SMRiQ8IXH7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NELF-D family.Curated

Phylogenomic databases

eggNOGiENOG410IFGF Eukaryota
ENOG410XSDJ LUCA
HOVERGENiHBG052597
InParanoidiQ8IXH7
KOiK15181
PhylomeDBiQ8IXH7
TreeFamiTF324118

Family and domain databases

InterProiView protein in InterPro
IPR006942 TH1
PANTHERiPTHR12144 PTHR12144, 1 hit
PfamiView protein in Pfam
PF04858 TH1, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform NELF-C (identifier: Q8IXH7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGAVPGAIM DEDYYGSAAE WGDEADGGQQ EDDSGEGEDD AEVQQECLHK
60 70 80 90 100
FSTRDYIMEP SIFNTLKRYF QAGGSPENVI QLLSENYTAV AQTVNLLAEW
110 120 130 140 150
LIQTGVEPVQ VQETVENHLK SLLIKHFDPR KADSIFTEEG ETPAWLEQMI
160 170 180 190 200
AHTTWRDLFY KLAEAHPDCL MLNFTVKLIS DAGYQGEITS VSTACQQLEV
210 220 230 240 250
FSRVLRTSLA TILDGGEENL EKNLPEFAKM VCHGEHTYLF AQAMMSVLAQ
260 270 280 290 300
EEQGGSAVRR IAQEVQRFAQ EKGHDASQIT LALGTAASYP RACQALGAML
310 320 330 340 350
SKGALNPADI TVLFKMFTSM DPPPVELIRV PAFLDLFMQS LFKPGARINQ
360 370 380 390 400
DHKHKYIHIL AYAASVVETW KKNKRVSINK DELKSTSKAV ETVHNLCCNE
410 420 430 440 450
NKGASELVAE LSTLYQCIRF PVVAMGVLKW VDWTVSEPRY FQLQTDHTPV
460 470 480 490 500
HLALLDEIST CHQLLHPQVL QLLVKLFETE HSQLDVMEQL ELKKTLLDRM
510 520 530 540 550
VHLLSRGYVL PVVSYIRKCL EKLDTDISLI RYFVTEVLDV IAPPYTSDFV
560 570 580 590
QLFLPILEND SIAGTIKTEG EHDPVTEFIA HCKSNFIMVN
Length:590
Mass (Da):66,247
Last modified:November 14, 2003 - v2
Checksum:i425437DC46DAF953
GO
Isoform 3 (identifier: Q8IXH7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-201: LISDAGYQGEITSVSTACQQLEVF → VGRVLELRRKVFMNVYFWLLVCFL
     202-590: Missing.

Note: No experimental confirmation available.
Show »
Length:201
Mass (Da):22,903
Checksum:i28EA9E5A52CD68FF
GO
Isoform NELF-D (identifier: Q8IXH7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Note: Produced by alternative initiation at Met-10 of isoform NELF-C.
Show »
Length:581
Mass (Da):65,479
Checksum:iDD20EFAF08DE67E8
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0UI80H0UI80_HUMAN
Negative elongation factor C/D
NELFCD TH1L, hCG_28547
599Annotation score:
X6RLT1X6RLT1_HUMAN
Negative elongation factor C/D
NELFCD
593Annotation score:

Sequence cautioni

The sequence AAF29094 differs from that shown. Reason: Frameshift at positions 462 and 464.Curated
The sequence BAA91618 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14519 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB64339 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117N → D in BAA91618 (PubMed:14702039).Curated1
Sequence conflicti306N → S in BAB14729 (PubMed:14702039).Curated1
Sequence conflicti430W → R in BAA91618 (PubMed:14702039).Curated1
Sequence conflicti497L → P in BAA91618 (PubMed:14702039).Curated1
Sequence conflicti573D → V in BAA91618 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187691 – 9Missing in isoform NELF-D. Curated9
Alternative sequenceiVSP_008950178 – 201LISDA…QLEVF → VGRVLELRRKVFMNVYFWLL VCFL in isoform 3. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_008951202 – 590Missing in isoform 3. 1 PublicationAdd BLAST389

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238374 mRNA Translation: CAB64337.1
AJ238375 mRNA Translation: CAB64373.1
AJ238379 mRNA Translation: CAB64339.1 Different initiation.
AF161479 mRNA Translation: AAF29094.1 Frameshift.
AK001316 mRNA Translation: BAA91618.1 Different initiation.
AK023310 mRNA Translation: BAB14519.1 Different initiation.
AK023927 mRNA Translation: BAB14729.1
AK293410 mRNA Translation: BAG56917.1
AL109840 Genomic DNA No translation available.
BC014952 mRNA Translation: AAH14952.1
CCDSiCCDS13473.2 [Q8IXH7-4]
RefSeqiNP_945327.2, NM_198976.2
UniGeneiHs.517148

Genome annotation databases

EnsembliENST00000344018; ENSP00000342300; ENSG00000101158
GeneIDi51497
KEGGihsa:51497
UCSCiuc061ydt.1 human [Q8IXH7-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238374 mRNA Translation: CAB64337.1
AJ238375 mRNA Translation: CAB64373.1
AJ238379 mRNA Translation: CAB64339.1 Different initiation.
AF161479 mRNA Translation: AAF29094.1 Frameshift.
AK001316 mRNA Translation: BAA91618.1 Different initiation.
AK023310 mRNA Translation: BAB14519.1 Different initiation.
AK023927 mRNA Translation: BAB14729.1
AK293410 mRNA Translation: BAG56917.1
AL109840 Genomic DNA No translation available.
BC014952 mRNA Translation: AAH14952.1
CCDSiCCDS13473.2 [Q8IXH7-4]
RefSeqiNP_945327.2, NM_198976.2
UniGeneiHs.517148

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5L3XX-ray2.75B186-590[»]
6GMLelectron microscopy3.20W10-590[»]
ProteinModelPortaliQ8IXH7
SMRiQ8IXH7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119572, 45 interactors
CORUMiQ8IXH7
DIPiDIP-34061N
IntActiQ8IXH7, 31 interactors
MINTiQ8IXH7
STRINGi9606.ENSP00000342300

PTM databases

iPTMnetiQ8IXH7
PhosphoSitePlusiQ8IXH7

Polymorphism and mutation databases

DMDMi38372376

Proteomic databases

EPDiQ8IXH7
MaxQBiQ8IXH7
PaxDbiQ8IXH7
PeptideAtlasiQ8IXH7
PRIDEiQ8IXH7
ProteomicsDBi70993
70994 [Q8IXH7-3]
70995 [Q8IXH7-4]

Protocols and materials databases

DNASUi51497
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344018; ENSP00000342300; ENSG00000101158
GeneIDi51497
KEGGihsa:51497
UCSCiuc061ydt.1 human [Q8IXH7-1]

Organism-specific databases

CTDi51497
DisGeNETi51497
EuPathDBiHostDB:ENSG00000101158.12
GeneCardsiNELFCD
H-InvDBiHIX0138057
HGNCiHGNC:15934 NELFCD
HPAiHPA050641
HPA066262
MIMi605297 gene
neXtProtiNX_Q8IXH7
PharmGKBiPA38055
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFGF Eukaryota
ENOG410XSDJ LUCA
HOVERGENiHBG052597
InParanoidiQ8IXH7
KOiK15181
PhylomeDBiQ8IXH7
TreeFamiTF324118

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

ChiTaRSiNELFCD human
GeneWikiiTH1L
GenomeRNAii51497
PROiPR:Q8IXH7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101158 Expressed in 222 organ(s), highest expression level in adenohypophysis
GenevisibleiQ8IXH7 HS

Family and domain databases

InterProiView protein in InterPro
IPR006942 TH1
PANTHERiPTHR12144 PTHR12144, 1 hit
PfamiView protein in Pfam
PF04858 TH1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNELFD_HUMAN
AccessioniPrimary (citable) accession number: Q8IXH7
Secondary accession number(s): B4DE06
, Q9BYL2, Q9H405, Q9H888, Q9H8T3, Q9NVX5, Q9P029, Q9UGN1, Q9UGN2, Q9UGN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again