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Protein

DnaJ homolog subfamily C member 10

Gene

DNAJC10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 10 (EC:1.8.4.-)
Alternative name(s):
Endoplasmic reticulum DNA J domain-containing protein 5
Short name:
ER-resident protein ERdj5
Short name:
ERdj5
Macrothioredoxin
Short name:
MTHr
Gene namesi
Name:DNAJC10
Synonyms:ERDJ5
ORF Names:UNQ495/PRO1012
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000077232.16
HGNCiHGNC:24637 DNAJC10
MIMi607987 gene
neXtProtiNX_Q8IXB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63H → Q: Prevents interaction with HSPA5, leading to prolonged interaction with substrate proteins. 1 Publication1
Mutagenesisi161C → A: Abolishes disulfide reductase activity; when associated with A-483; A-591 and A-703. 1 Publication1
Mutagenesisi483C → A: Abolishes disulfide reductase activity; when associated with A-161; A-591 and A-703. 1 Publication1
Mutagenesisi591C → A: Abolishes disulfide reductase activity; when associated with A-161; A-483 and A-703. 1 Publication1
Mutagenesisi703C → A: Abolishes disulfide reductase activity; when associated with A-161; A-483 and A-591. 1 Publication1

Organism-specific databases

DisGeNETi54431
OpenTargetsiENSG00000077232
PharmGKBiPA134917195

Polymorphism and mutation databases

DMDMi142981524

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000028148333 – 793DnaJ homolog subfamily C member 10Add BLAST761

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi158 ↔ 161Redox-active
Disulfide bondi480 ↔ 483Redox-active
Glycosylationi530N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi588 ↔ 591Redox-active
Disulfide bondi700 ↔ 703Redox-active

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8IXB1
MaxQBiQ8IXB1
PaxDbiQ8IXB1
PeptideAtlasiQ8IXB1
PRIDEiQ8IXB1
ProteomicsDBi70980
70981 [Q8IXB1-2]

PTM databases

iPTMnetiQ8IXB1
PhosphoSitePlusiQ8IXB1
SwissPalmiQ8IXB1

Expressioni

Inductioni

By endoplasmic reticulum stress.

Gene expression databases

BgeeiENSG00000077232 Expressed in 221 organ(s), highest expression level in caput epididymis
ExpressionAtlasiQ8IXB1 baseline and differential
GenevisibleiQ8IXB1 HS

Organism-specific databases

HPAiHPA031111

Interactioni

Subunit structurei

Interacts with EDEM1 (By similarity). Interacts with HSPA5 (via its J domain).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119947, 61 interactors
DIPiDIP-48947N
IntActiQ8IXB1, 19 interactors
STRINGi9606.ENSP00000264065

Structurei

3D structure databases

ProteinModelPortaliQ8IXB1
SMRiQ8IXB1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 100JPROSITE-ProRule annotationAdd BLAST66
Domaini130 – 232Thioredoxin 1PROSITE-ProRule annotationAdd BLAST103
Domaini454 – 553Thioredoxin 2PROSITE-ProRule annotationAdd BLAST100
Domaini557 – 662Thioredoxin 3PROSITE-ProRule annotationAdd BLAST106
Domaini671 – 778Thioredoxin 4PROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 350Trxb 1Add BLAST116
Regioni348 – 463Trxb 2Add BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi790 – 793Prevents secretion from ERPROSITE-ProRule annotation4

Domaini

The thioredoxin-like regions Trxb 1 and 2 lack a redox-active CXXC motif.By similarity
Thioredoxin domains 3 and 4 are the primary reductase domains.By similarity

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IMZF Eukaryota
KOG0191 Eukaryota
COG0526 LUCA
COG2214 LUCA
GeneTreeiENSGT00930000150840
HOVERGENiHBG057048
InParanoidiQ8IXB1
KOiK09530
OMAiAVNCGDN
OrthoDBiEOG091G0BFU
PhylomeDBiQ8IXB1
TreeFamiTF105169

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd03004 PDI_a_ERdj5_C, 3 hits
cd03003 PDI_a_ERdj5_N, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR021170 ERdj5
IPR035674 ERdj5_TRX_C
IPR035673 ERdj5_TRX_N
IPR036869 J_dom_sf
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF00085 Thioredoxin, 4 hits
PIRSFiPIRSF037293 DnaJ_homolog_subfam-C, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF52833 SSF52833, 6 hits
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 3 hits

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IXB1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGVWLNKDDY IRDLKRIILC FLIVYMAILV GTDQDFYSLL GVSKTASSRE
60 70 80 90 100
IRQAFKKLAL KLHPDKNPNN PNAHGDFLKI NRAYEVLKDE DLRKKYDKYG
110 120 130 140 150
EKGLEDNQGG QYESWNYYRY DFGIYDDDPE IITLERREFD AAVNSGELWF
160 170 180 190 200
VNFYSPGCSH CHDLAPTWRD FAKEVDGLLR IGAVNCGDDR MLCRMKGVNS
210 220 230 240 250
YPSLFIFRSG MAPVKYHGDR SKESLVSFAM QHVRSTVTEL WTGNFVNSIQ
260 270 280 290 300
TAFAAGIGWL ITFCSKGGDC LTSQTRLRLS GMLDGLVNVG WMDCATQDNL
310 320 330 340 350
CKSLDITTST TAYFPPGATL NNKEKNSILF LNSLDAKEIY LEVIHNLPDF
360 370 380 390 400
ELLSANTLED RLAHHRWLLF FHFGKNENSN DPELKKLKTL LKNDHIQVGR
410 420 430 440 450
FDCSSAPDIC SNLYVFQPSL AVFKGQGTKE YEIHHGKKIL YDILAFAKES
460 470 480 490 500
VNSHVTTLGP QNFPANDKEP WLVDFFAPWC PPCRALLPEL RRASNLLYGQ
510 520 530 540 550
LKFGTLDCTV HEGLCNMYNI QAYPTTVVFN QSNIHEYEGH HSAEQILEFI
560 570 580 590 600
EDLMNPSVVS LTPTTFNELV TQRKHNEVWM VDFYSPWCHP CQVLMPEWKR
610 620 630 640 650
MARTLTGLIN VGSIDCQQYH SFCAQENVQR YPEIRFFPPK SNKAYHYHSY
660 670 680 690 700
NGWNRDAYSL RIWGLGFLPQ VSTDLTPQTF SEKVLQGKNH WVIDFYAPWC
710 720 730 740 750
GPCQNFAPEF ELLARMIKGK VKAGKVDCQA YAQTCQKAGI RAYPTVKFYF
760 770 780 790
YERAKRNFQE EQINTRDAKA IAALISEKLE TLRNQGKRNK DEL
Length:793
Mass (Da):91,080
Last modified:April 3, 2007 - v2
Checksum:iBD39B64325432D5D
GO
Isoform 2 (identifier: Q8IXB1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-329: Missing.

Show »
Length:747
Mass (Da):86,136
Checksum:iD9F26A53E1B35455
GO
Isoform 3 (identifier: Q8IXB1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     330-332: FLN → LLH
     333-793: Missing.

Note: No experimental confirmation available.
Show »
Length:332
Mass (Da):37,682
Checksum:i256FEF2695B28BE7
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EP04E7EP04_HUMAN
DnaJ homolog subfamily C member 10
DNAJC10
275Annotation score:
Q71S60Q71S60_HUMAN
DnaJ homolog subfamily C member 10
DNAJC10
177Annotation score:
A0A087WXH7A0A087WXH7_HUMAN
DnaJ homolog subfamily C member 10
DNAJC10
177Annotation score:
H7C1G0H7C1G0_HUMAN
DnaJ homolog subfamily C member 10
DNAJC10
121Annotation score:

Sequence cautioni

The sequence BAB55121 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC11281 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5L → S in AAM09527 (PubMed:14587667).Curated1
Sequence conflicti5L → S in BAB55304 (PubMed:14702039).Curated1
Sequence conflicti213P → A in AAN73271 (PubMed:12411443).Curated1
Sequence conflicti356 – 359NTLE → KRVK in AAN73271 (PubMed:12411443).Curated4
Sequence conflicti446F → S in BAB55121 (PubMed:14702039).Curated1
Sequence conflicti565T → A in CAD89982 (PubMed:17974005).Curated1
Sequence conflicti633E → K in CAB70858 (PubMed:17974005).Curated1
Sequence conflicti755K → N in BAB55304 (PubMed:14702039).Curated1
Sequence conflicti771I → T in CAD89982 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03124776D → N2 PublicationsCorresponds to variant dbSNP:rs6729801Ensembl.1
Natural variantiVAR_048912347L → I. Corresponds to variant dbSNP:rs13414223Ensembl.1
Natural variantiVAR_031248414Y → C. Corresponds to variant dbSNP:rs11681366Ensembl.1
Natural variantiVAR_031249646H → Q3 PublicationsCorresponds to variant dbSNP:rs288334Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024011284 – 329Missing in isoform 2. 2 PublicationsAdd BLAST46
Alternative sequenceiVSP_054434330 – 332FLN → LLH in isoform 3. 1 Publication3
Alternative sequenceiVSP_054435333 – 793Missing in isoform 3. 1 PublicationAdd BLAST461

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038503 mRNA Translation: AAN73271.1
AF490904 mRNA Translation: AAM09527.1
AY358577 mRNA Translation: AAQ88940.1
AK027450 mRNA Translation: BAB55121.1 Different initiation.
AK027647 mRNA Translation: BAB55263.1
AK027696 mRNA Translation: BAB55304.1
AC073951 Genomic DNA Translation: AAX88931.1
AC105396 Genomic DNA Translation: AAY24240.1
CH471058 Genomic DNA Translation: EAX10960.1
CH471058 Genomic DNA Translation: EAX10963.1
BC107425 mRNA Translation: AAI07426.1
BC117299 mRNA Translation: AAI17300.1
BC126168 mRNA Translation: AAI26169.1
AL137648 mRNA Translation: CAB70858.1
AL832646 mRNA Translation: CAD89982.1
AK074905 mRNA Translation: BAC11281.1 Different initiation.
CCDSiCCDS33345.1 [Q8IXB1-1]
CCDS74613.1 [Q8IXB1-2]
PIRiT46333
RefSeqiNP_001258510.1, NM_001271581.1 [Q8IXB1-2]
NP_061854.1, NM_018981.2 [Q8IXB1-1]
UniGeneiHs.516632
Hs.744512

Genome annotation databases

EnsembliENST00000264065; ENSP00000264065; ENSG00000077232 [Q8IXB1-1]
ENST00000537515; ENSP00000441560; ENSG00000077232 [Q8IXB1-3]
ENST00000616986; ENSP00000479930; ENSG00000077232 [Q8IXB1-2]
GeneIDi54431
KEGGihsa:54431
UCSCiuc002uow.3 human [Q8IXB1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038503 mRNA Translation: AAN73271.1
AF490904 mRNA Translation: AAM09527.1
AY358577 mRNA Translation: AAQ88940.1
AK027450 mRNA Translation: BAB55121.1 Different initiation.
AK027647 mRNA Translation: BAB55263.1
AK027696 mRNA Translation: BAB55304.1
AC073951 Genomic DNA Translation: AAX88931.1
AC105396 Genomic DNA Translation: AAY24240.1
CH471058 Genomic DNA Translation: EAX10960.1
CH471058 Genomic DNA Translation: EAX10963.1
BC107425 mRNA Translation: AAI07426.1
BC117299 mRNA Translation: AAI17300.1
BC126168 mRNA Translation: AAI26169.1
AL137648 mRNA Translation: CAB70858.1
AL832646 mRNA Translation: CAD89982.1
AK074905 mRNA Translation: BAC11281.1 Different initiation.
CCDSiCCDS33345.1 [Q8IXB1-1]
CCDS74613.1 [Q8IXB1-2]
PIRiT46333
RefSeqiNP_001258510.1, NM_001271581.1 [Q8IXB1-2]
NP_061854.1, NM_018981.2 [Q8IXB1-1]
UniGeneiHs.516632
Hs.744512

3D structure databases

ProteinModelPortaliQ8IXB1
SMRiQ8IXB1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119947, 61 interactors
DIPiDIP-48947N
IntActiQ8IXB1, 19 interactors
STRINGi9606.ENSP00000264065

PTM databases

iPTMnetiQ8IXB1
PhosphoSitePlusiQ8IXB1
SwissPalmiQ8IXB1

Polymorphism and mutation databases

DMDMi142981524

Proteomic databases

EPDiQ8IXB1
MaxQBiQ8IXB1
PaxDbiQ8IXB1
PeptideAtlasiQ8IXB1
PRIDEiQ8IXB1
ProteomicsDBi70980
70981 [Q8IXB1-2]

Protocols and materials databases

DNASUi54431
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264065; ENSP00000264065; ENSG00000077232 [Q8IXB1-1]
ENST00000537515; ENSP00000441560; ENSG00000077232 [Q8IXB1-3]
ENST00000616986; ENSP00000479930; ENSG00000077232 [Q8IXB1-2]
GeneIDi54431
KEGGihsa:54431
UCSCiuc002uow.3 human [Q8IXB1-1]

Organism-specific databases

CTDi54431
DisGeNETi54431
EuPathDBiHostDB:ENSG00000077232.16
GeneCardsiDNAJC10
HGNCiHGNC:24637 DNAJC10
HPAiHPA031111
MIMi607987 gene
neXtProtiNX_Q8IXB1
OpenTargetsiENSG00000077232
PharmGKBiPA134917195
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IMZF Eukaryota
KOG0191 Eukaryota
COG0526 LUCA
COG2214 LUCA
GeneTreeiENSGT00930000150840
HOVERGENiHBG057048
InParanoidiQ8IXB1
KOiK09530
OMAiAVNCGDN
OrthoDBiEOG091G0BFU
PhylomeDBiQ8IXB1
TreeFamiTF105169

Miscellaneous databases

ChiTaRSiDNAJC10 human
GeneWikiiDNAJC10
GenomeRNAii54431
PROiPR:Q8IXB1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000077232 Expressed in 221 organ(s), highest expression level in caput epididymis
ExpressionAtlasiQ8IXB1 baseline and differential
GenevisibleiQ8IXB1 HS

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd03004 PDI_a_ERdj5_C, 3 hits
cd03003 PDI_a_ERdj5_N, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR021170 ERdj5
IPR035674 ERdj5_TRX_C
IPR035673 ERdj5_TRX_N
IPR036869 J_dom_sf
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF00085 Thioredoxin, 4 hits
PIRSFiPIRSF037293 DnaJ_homolog_subfam-C, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF52833 SSF52833, 6 hits
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiDJC10_HUMAN
AccessioniPrimary (citable) accession number: Q8IXB1
Secondary accession number(s): Q17RJ6
, Q3B7W8, Q4ZG06, Q53QT7, Q6UWZ6, Q86T61, Q8NC82, Q8TD87, Q96K38, Q96K44, Q96K54, Q9NSY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: November 7, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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