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Protein

Sperm acrosome membrane-associated protein 3

Gene

SPACA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.1 Publication

Caution

Although it belongs to the glycosyl hydrolase 22 family, Thr-122 and Asn-139 are present instead of the conserved Glu and Asp which are active site residues. It is therefore expected that this protein lacks hydrolase activity.Curated

GO - Biological processi

Protein family/group databases

CAZyiGH22 Glycoside Hydrolase Family 22

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm acrosome membrane-associated protein 3
Alternative name(s):
Cancer/testis antigen 54
Short name:
CT54
Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17
Lysozyme-like protein 3
Sperm lysozyme-like protein 1
Sperm protein reactive with antisperm antibodies
Short name:
Sperm protein reactive with ASA
Cleaved into the following 2 chains:
Gene namesi
Name:SPACA3
Synonyms:LYC3, LYZL3, SLLP1, SPRASA
ORF Names:UNQ424/PRO862
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000141316.12
HGNCiHGNC:16260 SPACA3
MIMi612749 gene
neXtProtiNX_Q8IXA5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 63CytoplasmicSequence analysisAdd BLAST63
Transmembranei64 – 84Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini85 – 215ExtracellularSequence analysisAdd BLAST131

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi124912
OpenTargetsiENSG00000141316
PharmGKBiPA38101

Polymorphism and mutation databases

BioMutaiSPACA3
DMDMi74723659

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002562191 – 215Sperm acrosome membrane-associated protein 3, membrane formAdd BLAST215
ChainiPRO_000025622088 – 215Sperm acrosome membrane-associated protein 3, processed formAdd BLAST128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi93 ↔ 213PROSITE-ProRule annotation
Disulfide bondi117 ↔ 201PROSITE-ProRule annotation
Disulfide bondi151 ↔ 166PROSITE-ProRule annotation
Disulfide bondi162 ↔ 180PROSITE-ProRule annotation

Post-translational modificationi

The processed form derives from the membrane form by proteolytic processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87 – 88Cleavage; to produce processed formCurated2

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8IXA5
PeptideAtlasiQ8IXA5
PRIDEiQ8IXA5
ProteomicsDBi70978
70979 [Q8IXA5-2]

PTM databases

iPTMnetiQ8IXA5
PhosphoSitePlusiQ8IXA5

Expressioni

Tissue specificityi

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.2 Publications

Gene expression databases

BgeeiENSG00000141316 Expressed in 59 organ(s), highest expression level in right testis
CleanExiHS_SPACA3
ExpressionAtlasiQ8IXA5 baseline and differential
GenevisibleiQ8IXA5 HS

Organism-specific databases

HPAiHPA023633

Interactioni

Subunit structurei

Interacts with ASTL.By similarity

Protein-protein interaction databases

BioGridi125897, 6 interactors
IntActiQ8IXA5, 2 interactors
STRINGi9606.ENSP00000269053

Structurei

3D structure databases

ProteinModelPortaliQ8IXA5
SMRiQ8IXA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 22 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG3E Eukaryota
ENOG41118PT LUCA
GeneTreeiENSGT00550000074398
HOGENOMiHOG000037357
HOVERGENiHBG052297
InParanoidiQ8IXA5
OMAiWICLAYF
OrthoDBiEOG091G0R9V
PhylomeDBiQ8IXA5
TreeFamiTF324882

Family and domain databases

CDDicd00119 LYZ1, 1 hit
InterProiView protein in InterPro
IPR001916 Glyco_hydro_22
IPR019799 Glyco_hydro_22_CS
IPR000974 Glyco_hydro_22_lys
IPR023346 Lysozyme-like_dom_sf
IPR030058 LYZL3
PANTHERiPTHR11407:SF25 PTHR11407:SF25, 1 hit
PfamiView protein in Pfam
PF00062 Lys, 1 hit
PRINTSiPR00137 LYSOZYME
PR00135 LYZLACT
SMARTiView protein in SMART
SM00263 LYZ1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS00128 LACTALBUMIN_LYSOZYME_1, 1 hit
PS51348 LACTALBUMIN_LYSOZYME_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IXA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVSALRGAPL IRVHSSPVSS PSVSGPRRLV SCLSSQSSAL SQSGGGSTSA
60 70 80 90 100
AGIEARSRAL RRRWCPAGIM LLALVCLLSC LLPSSEAKLY GRCELARVLH
110 120 130 140 150
DFGLDGYRGY SLADWVCLAY FTSGFNAAAL DYEADGSTNN GIFQINSRRW
160 170 180 190 200
CSNLTPNVPN VCRMYCSDLL NPNLKDTVIC AMKITQEPQG LGYWEAWRHH
210
CQGKDLTEWV DGCDF
Length:215
Mass (Da):23,431
Last modified:March 1, 2003 - v1
Checksum:i39988565D592BCC8
GO
Isoform 2 (identifier: Q8IXA5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.

Show »
Length:146
Mass (Da):16,430
Checksum:i58CE171F2581158C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PF91E9PF91_HUMAN
Sperm acrosome membrane-associated ...
SPACA3
112Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02888580C → Y. Corresponds to variant dbSNP:rs16967845Ensembl.1
Natural variantiVAR_028886100H → R. Corresponds to variant dbSNP:rs28963Ensembl.1
Natural variantiVAR_050008128A → T. Corresponds to variant dbSNP:rs35420663Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0213291 – 69Missing in isoform 2. 1 PublicationAdd BLAST69

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216311 mRNA Translation: AAK01478.1
AF099029 Genomic DNA Translation: AAP97222.1
AY358653 mRNA Translation: AAQ89016.1
BC100886 mRNA Translation: AAI00887.1
BC100887 mRNA Translation: AAI00888.1
CCDSiCCDS11275.1 [Q8IXA5-1]
RefSeqiNP_001304155.1, NM_001317226.1
NP_776246.1, NM_173847.4 [Q8IXA5-1]
UniGeneiHs.434112

Genome annotation databases

EnsembliENST00000269053; ENSP00000269053; ENSG00000141316 [Q8IXA5-1]
ENST00000580599; ENSP00000463386; ENSG00000141316 [Q8IXA5-2]
GeneIDi124912
KEGGihsa:124912
UCSCiuc002hhs.2 human [Q8IXA5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216311 mRNA Translation: AAK01478.1
AF099029 Genomic DNA Translation: AAP97222.1
AY358653 mRNA Translation: AAQ89016.1
BC100886 mRNA Translation: AAI00887.1
BC100887 mRNA Translation: AAI00888.1
CCDSiCCDS11275.1 [Q8IXA5-1]
RefSeqiNP_001304155.1, NM_001317226.1
NP_776246.1, NM_173847.4 [Q8IXA5-1]
UniGeneiHs.434112

3D structure databases

ProteinModelPortaliQ8IXA5
SMRiQ8IXA5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125897, 6 interactors
IntActiQ8IXA5, 2 interactors
STRINGi9606.ENSP00000269053

Protein family/group databases

CAZyiGH22 Glycoside Hydrolase Family 22

PTM databases

iPTMnetiQ8IXA5
PhosphoSitePlusiQ8IXA5

Polymorphism and mutation databases

BioMutaiSPACA3
DMDMi74723659

Proteomic databases

PaxDbiQ8IXA5
PeptideAtlasiQ8IXA5
PRIDEiQ8IXA5
ProteomicsDBi70978
70979 [Q8IXA5-2]

Protocols and materials databases

DNASUi124912
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000269053; ENSP00000269053; ENSG00000141316 [Q8IXA5-1]
ENST00000580599; ENSP00000463386; ENSG00000141316 [Q8IXA5-2]
GeneIDi124912
KEGGihsa:124912
UCSCiuc002hhs.2 human [Q8IXA5-1]

Organism-specific databases

CTDi124912
DisGeNETi124912
EuPathDBiHostDB:ENSG00000141316.12
GeneCardsiSPACA3
H-InvDBiHIX0039076
HGNCiHGNC:16260 SPACA3
HPAiHPA023633
MIMi612749 gene
neXtProtiNX_Q8IXA5
OpenTargetsiENSG00000141316
PharmGKBiPA38101
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG3E Eukaryota
ENOG41118PT LUCA
GeneTreeiENSGT00550000074398
HOGENOMiHOG000037357
HOVERGENiHBG052297
InParanoidiQ8IXA5
OMAiWICLAYF
OrthoDBiEOG091G0R9V
PhylomeDBiQ8IXA5
TreeFamiTF324882

Miscellaneous databases

ChiTaRSiSPACA3 human
GeneWikiiSPACA3
GenomeRNAii124912
PROiPR:Q8IXA5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141316 Expressed in 59 organ(s), highest expression level in right testis
CleanExiHS_SPACA3
ExpressionAtlasiQ8IXA5 baseline and differential
GenevisibleiQ8IXA5 HS

Family and domain databases

CDDicd00119 LYZ1, 1 hit
InterProiView protein in InterPro
IPR001916 Glyco_hydro_22
IPR019799 Glyco_hydro_22_CS
IPR000974 Glyco_hydro_22_lys
IPR023346 Lysozyme-like_dom_sf
IPR030058 LYZL3
PANTHERiPTHR11407:SF25 PTHR11407:SF25, 1 hit
PfamiView protein in Pfam
PF00062 Lys, 1 hit
PRINTSiPR00137 LYSOZYME
PR00135 LYZLACT
SMARTiView protein in SMART
SM00263 LYZ1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS00128 LACTALBUMIN_LYSOZYME_1, 1 hit
PS51348 LACTALBUMIN_LYSOZYME_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSACA3_HUMAN
AccessioniPrimary (citable) accession number: Q8IXA5
Secondary accession number(s): Q7Z4Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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