UniProtKB - Q8IWL3 (HSC20_HUMAN)
Iron-sulfur cluster co-chaperone protein HscB
HSCB
Functioni
: iron-sulfur cluster biosynthesis Pathwayi
This protein is involved in the pathway iron-sulfur cluster biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway iron-sulfur cluster biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 41 | Divalent metal cationCombined sources1 Publication | 1 | |
Metal bindingi | 44 | Divalent metal cationCombined sources1 Publication | 1 | |
Metal bindingi | 58 | Divalent metal cationCombined sources1 Publication | 1 | |
Metal bindingi | 61 | Divalent metal cationCombined sources1 Publication | 1 |
GO - Molecular functioni
- ATPase activator activity Source: InterPro
- chaperone binding Source: InterPro
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- [2Fe-2S] cluster assembly Source: GO_Central
- iron-sulfur cluster assembly Source: UniProtKB
- protein complex oligomerization Source: InterPro
- protein maturation by iron-sulfur cluster transfer Source: InterPro
Keywordsi
Molecular function | Chaperone |
Ligand | Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8IWL3 |
Reactomei | R-HSA-1268020, Mitochondrial protein import R-HSA-1362409, Mitochondrial iron-sulfur cluster biogenesis |
UniPathwayi | UPA00266 |
Names & Taxonomyi
Protein namesi | Recommended name: Iron-sulfur cluster co-chaperone protein HscBAlternative name(s): DnaJ homolog subfamily C member 20 Cleaved into the following 2 chains: |
Gene namesi | Name:HSCB Synonyms:DNAJC20, HSC20 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28913, HSCB |
MIMi | 608142, gene |
neXtProti | NX_Q8IWL3 |
VEuPathDBi | HostDB:ENSG00000100209.9 |
Subcellular locationi
Other locations
- Cytoplasm 2 Publications
Mitochondrion
- Mitochondrion 1 Publication
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 41 | C → S: Abolishes self-interaction and interaction with HSPA9 and the CIA complex but does not alter subcellular localization; when associated with S-44; S-58 and S-61. 2 Publications | 1 | |
Mutagenesisi | 44 | C → S: Abolishes self-interaction and interaction with HSPA9 and the CIA complex but does not alter subcellular localization; when associated with S-41; S-58 and S-61. 2 Publications | 1 | |
Mutagenesisi | 58 | C → S: Abolishes self-interaction and interaction with HSPA9 and the CIA complex but does not alter subcellular localization; when associated with S-41; S-44 and S-61. 2 Publications | 1 | |
Mutagenesisi | 61 | C → S: Abolishes self-interaction and interaction with HSPA9 and the CIA complex but does not alter subcellular localization; when associated with S-41; S-44 and S-58. 2 Publications | 1 | |
Mutagenesisi | 102 – 104 | HPD → AAA: Does not interact with HSPA9. Does not inhibit interaction with ISCU. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 150274 |
OpenTargetsi | ENSG00000100209 |
PharmGKBi | PA162391621 |
Miscellaneous databases
Pharosi | Q8IWL3, Tbio |
Genetic variation databases
BioMutai | HSCB |
DMDMi | 60416441 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000446242 | 1 – 235 | Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic1 PublicationAdd BLAST | 235 | |
ChainiPRO_0000007262 | 30 – 235 | Iron-sulfur cluster co-chaperone protein HscB, mitochondrialSequence analysisAdd BLAST | 206 |
Proteomic databases
EPDi | Q8IWL3 |
jPOSTi | Q8IWL3 |
MassIVEi | Q8IWL3 |
MaxQBi | Q8IWL3 |
PaxDbi | Q8IWL3 |
PeptideAtlasi | Q8IWL3 |
PRIDEi | Q8IWL3 |
ProteomicsDBi | 70870 |
PTM databases
iPTMneti | Q8IWL3 |
PhosphoSitePlusi | Q8IWL3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000100209, Expressed in parotid gland and 213 other tissues |
ExpressionAtlasi | Q8IWL3, baseline and differential |
Genevisiblei | Q8IWL3, HS |
Organism-specific databases
HPAi | ENSG00000100209, Low tissue specificity |
Interactioni
Subunit structurei
Self-interacts (PubMed:29309586).
Interacts with ISCU and HSPA9 to form an iron-sulfur transfer complex (PubMed:20668094).
Interacts with SDHAF1 (via the first LYR motif); the interaction recruits the iron-sulfur transfer complex composed of HSC20, HSPA9 and ISCU and mediates the incorporation of iron-sulfur clusters into SDHB which also interacts with HSC20 (PubMed:26749241).
Interacts with the cytoplasmic form of ISCU and with CIA complex member CIAO1 (via LYR motif) (PubMed:29309586).
3 PublicationsBinary interactionsi
Hide detailsQ8IWL3
GO - Molecular functioni
- chaperone binding Source: InterPro
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 127276, 13 interactors |
DIPi | DIP-46570N |
IntActi | Q8IWL3, 738 interactors |
STRINGi | 9606.ENSP00000216027 |
Miscellaneous databases
RNActi | Q8IWL3, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8IWL3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8IWL3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 72 – 144 | JAdd BLAST | 73 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3192, Eukaryota |
GeneTreei | ENSGT00390000008206 |
HOGENOMi | CLU_068529_0_2_1 |
InParanoidi | Q8IWL3 |
OMAi | KFMAKLQ |
OrthoDBi | 1129824at2759 |
PhylomeDBi | Q8IWL3 |
TreeFami | TF319992 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit 1.20.1280.20, 1 hit |
HAMAPi | MF_00682, HscB, 1 hit |
InterProi | View protein in InterPro IPR001623, DnaJ_domain IPR004640, HscB IPR040682, HscB_4_cys IPR036386, HscB_C_sf IPR009073, HscB_oligo_C IPR036869, J_dom_sf |
PANTHERi | PTHR14021, PTHR14021, 1 hit |
Pfami | View protein in Pfam PF18256, HscB_4_cys, 1 hit PF07743, HSCB_C, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF47144, SSF47144, 1 hit |
TIGRFAMsi | TIGR00714, hscB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MWRGRAGALL RVWGFWPTGV PRRRPLSCDA ASQAGSNYPR CWNCGGPWGP
60 70 80 90 100
GREDRFFCPQ CRALQAPDPT RDYFSLMDCN RSFRVDTAKL QHRYQQLQRL
110 120 130 140 150
VHPDFFSQRS QTEKDFSEKH STLVNDAYKT LLAPLSRGLY LLKLHGIEIP
160 170 180 190 200
ERTDYEMDRQ FLIEIMEINE KLAEAESEAA MKEIESIVKA KQKEFTDNVS
210 220 230
SAFEQDDFEE AKEILTKMRY FSNIEEKIKL KKIPL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB0QYH2 | B0QYH2_HUMAN | Iron-sulfur cluster co-chaperone pr... | HSCB RP3-366L4.2, hCG_40981 | 143 | Annotation score: | ||
F8WBY7 | F8WBY7_HUMAN | Iron-sulfur cluster co-chaperone pr... | HSCB | 105 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 | N → S in AAN85282 (PubMed:12938016).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048916 | 73 | Y → C. Corresponds to variant dbSNP:rs17886090Ensembl. | 1 | |
Natural variantiVAR_048917 | 163 | I → M. Corresponds to variant dbSNP:rs17884212Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY191719 mRNA Translation: AAN85282.1 CR456462 mRNA Translation: CAG30348.1 AL023494 Genomic DNA No translation available. AL117330 Genomic DNA No translation available. BC065569 mRNA Translation: AAH65569.1 |
CCDSi | CCDS13845.1 |
RefSeqi | NP_741999.3, NM_172002.4 |
Genome annotation databases
Ensembli | ENST00000216027; ENSP00000216027; ENSG00000100209 |
GeneIDi | 150274 |
KEGGi | hsa:150274 |
UCSCi | uc003aea.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY191719 mRNA Translation: AAN85282.1 CR456462 mRNA Translation: CAG30348.1 AL023494 Genomic DNA No translation available. AL117330 Genomic DNA No translation available. BC065569 mRNA Translation: AAH65569.1 |
CCDSi | CCDS13845.1 |
RefSeqi | NP_741999.3, NM_172002.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3BVO | X-ray | 3.00 | A/B | 30-235 | [»] | |
SMRi | Q8IWL3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127276, 13 interactors |
DIPi | DIP-46570N |
IntActi | Q8IWL3, 738 interactors |
STRINGi | 9606.ENSP00000216027 |
PTM databases
iPTMneti | Q8IWL3 |
PhosphoSitePlusi | Q8IWL3 |
Genetic variation databases
BioMutai | HSCB |
DMDMi | 60416441 |
Proteomic databases
EPDi | Q8IWL3 |
jPOSTi | Q8IWL3 |
MassIVEi | Q8IWL3 |
MaxQBi | Q8IWL3 |
PaxDbi | Q8IWL3 |
PeptideAtlasi | Q8IWL3 |
PRIDEi | Q8IWL3 |
ProteomicsDBi | 70870 |
Protocols and materials databases
Antibodypediai | 10209, 115 antibodies |
DNASUi | 150274 |
Genome annotation databases
Ensembli | ENST00000216027; ENSP00000216027; ENSG00000100209 |
GeneIDi | 150274 |
KEGGi | hsa:150274 |
UCSCi | uc003aea.4, human |
Organism-specific databases
CTDi | 150274 |
DisGeNETi | 150274 |
GeneCardsi | HSCB |
HGNCi | HGNC:28913, HSCB |
HPAi | ENSG00000100209, Low tissue specificity |
MIMi | 608142, gene |
neXtProti | NX_Q8IWL3 |
OpenTargetsi | ENSG00000100209 |
PharmGKBi | PA162391621 |
VEuPathDBi | HostDB:ENSG00000100209.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3192, Eukaryota |
GeneTreei | ENSGT00390000008206 |
HOGENOMi | CLU_068529_0_2_1 |
InParanoidi | Q8IWL3 |
OMAi | KFMAKLQ |
OrthoDBi | 1129824at2759 |
PhylomeDBi | Q8IWL3 |
TreeFami | TF319992 |
Enzyme and pathway databases
UniPathwayi | UPA00266 |
PathwayCommonsi | Q8IWL3 |
Reactomei | R-HSA-1268020, Mitochondrial protein import R-HSA-1362409, Mitochondrial iron-sulfur cluster biogenesis |
Miscellaneous databases
BioGRID-ORCSi | 150274, 521 hits in 888 CRISPR screens |
ChiTaRSi | HSCB, human |
EvolutionaryTracei | Q8IWL3 |
GenomeRNAii | 150274 |
Pharosi | Q8IWL3, Tbio |
PROi | PR:Q8IWL3 |
RNActi | Q8IWL3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100209, Expressed in parotid gland and 213 other tissues |
ExpressionAtlasi | Q8IWL3, baseline and differential |
Genevisiblei | Q8IWL3, HS |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit 1.20.1280.20, 1 hit |
HAMAPi | MF_00682, HscB, 1 hit |
InterProi | View protein in InterPro IPR001623, DnaJ_domain IPR004640, HscB IPR040682, HscB_4_cys IPR036386, HscB_C_sf IPR009073, HscB_oligo_C IPR036869, J_dom_sf |
PANTHERi | PTHR14021, PTHR14021, 1 hit |
Pfami | View protein in Pfam PF18256, HscB_4_cys, 1 hit PF07743, HSCB_C, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF47144, SSF47144, 1 hit |
TIGRFAMsi | TIGR00714, hscB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HSC20_HUMAN | |
Accessioni | Q8IWL3Primary (citable) accession number: Q8IWL3 Secondary accession number(s): Q9BWS7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | March 1, 2005 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families