UniProtKB - Q8IWE4 (DCNL3_HUMAN)
DCN1-like protein 3
DCUN1D3
Functioni
GO - Molecular functioni
- cullin family protein binding Source: UniProtKB
- ubiquitin conjugating enzyme binding Source: GO_Central
- ubiquitin-like protein binding Source: GO_Central
GO - Biological processi
- negative regulation of cell growth Source: UniProtKB
- negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
- negative regulation of protein neddylation Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of protein neddylation Source: UniProtKB
- positive regulation of ubiquitin-protein transferase activity Source: GO_Central
- post-translational protein modification Source: Reactome
- protein neddylation Source: GO_Central
- regulation of cell cycle process Source: UniProtKB
- regulation of protein neddylation Source: UniProtKB
- response to gamma radiation Source: UniProtKB
- response to UV-C Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q8IWE4 |
Reactomei | R-HSA-8951664, Neddylation |
Names & Taxonomyi
Protein namesi | Recommended name: DCN1-like protein 3Short name: DCNL31 Publication Alternative name(s): DCUN1 domain-containing protein 3 Defective in cullin neddylation protein 1-like protein 3 Squamous cell carcinoma-related oncogene 31 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28734, DCUN1D3 |
MIMi | 616167, gene |
neXtProti | NX_Q8IWE4 |
VEuPathDBi | HostDB:ENSG00000188215.9 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Nucleus
- Nucleus 2 Publications
Plasma membrane
- Cell membrane 3 Publications
Other locations
- perinuclear region 1 Publication
Note: After UVC treatment, the protein enters to the nucleus gradually (PubMed:18823379). Cell membrane localization is essential for CUL3 neddylation (PubMed:19617556).2 Publications
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 26 | Missing : No effect on CAND1-, CUL1-, CUL3- and RBX1-binding. 1 PublicationAdd BLAST | 26 | |
Mutagenesisi | 2 | G → A: No effect on CAND1-, CUL1-, CUL3- and RBX1-binding. Loss of localization at the cell membrane. Loss of function of inhibition of DCUN1D1-mediated CUL1 neddylation. Loss of myristoylation. Loss of myristoylation; when associated with A-4. Loss of myristoylation; when associated with A-8. Loss of palmitoylation. Affects cell membrane localization, and localizes throughout the cytoplasm. Does not relocalizes CUL3 to the cell membrane. 2 Publications | 1 | |
Mutagenesisi | 4 | C → A: Does not affect myristoylation; when associated with A-8. Loss of myristoylation; when associated with A-2. Loss of palmitoylation; when associated with A-8. Reduces cell membrane localization. 1 Publication | 1 | |
Mutagenesisi | 8 | C → A: Does not affect myristoylation; when associated with A-4. Loss of myristoylation; when associated with A-2. Loss of palmitoylation; when associated with A-4. Reduces cell membrane localization. 1 Publication | 1 | |
Mutagenesisi | 241 | D → A: Loss of interaction with CUL1, CUL2, CUL3, CULA4, CULA5, CAND1 and RBX1; when associated with R-265 and A-271. Does not affect both nucleus and cytoplasm localization; when associated with R-265 and A-271. Loss of interaction with CUL3; when associated with R-265 and A-271. 2 Publications | 1 | |
Mutagenesisi | 241 | D → N: Loss of CAND1-, CUL1-, CUL3- and RBX1-binding. Loss of function of inhibition of DCUN1D1-mediated CUL1 neddylation, but no effect on localization at the cell membrane; when associated with R-265 and N-271. 1 Publication | 1 | |
Mutagenesisi | 265 | A → R: Loss of CAND1-, CUL1-, CUL3- and RBX1-binding. Loss of function of inhibition of DCUN1D1-mediated CUL1 neddylation, but no effect on localization at the cell membrane; when associated with N-241 and N-271. Loss of interaction with CUL1, CUL2, CUL3, CULA4, CULA5, CAND1 and RBX1; when associated with A-241 and A-271. Does not affect both nucleus and cytoplasm localization; when associated with A-241 and A-271. Loss of interaction with CUL3; when associated with A-241 and A-271. 3 Publications | 1 | |
Mutagenesisi | 271 | D → A: Loss of interaction with CUL1, CUL2, CUL3, CULA4, CULA5 CALD1 and RBX1; when associated with A-241 and R-265. Does not affect both nucleus and cytoplasm localization; when associated with A-241 and R-265. Loss of interaction with CUL3; when associated with A-241 and A-271. 2 Publications | 1 | |
Mutagenesisi | 271 | D → N: Loss of CAND1-, CUL1-, CUL3- and RBX1-binding. Loss of function of inhibition of DCUN1D1-mediated CUL1 neddylation, but no effect on localization at the cell membrane; when associated with N-241 and R-265. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 123879 |
OpenTargetsi | ENSG00000188215 |
PharmGKBi | PA142672009 |
Miscellaneous databases
Pharosi | Q8IWE4, Tdark |
Chemistry databases
ChEMBLi | CHEMBL4295894 |
Genetic variation databases
BioMutai | DCUN1D3 |
DMDMi | 74728175 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedSequence analysis | |||
ChainiPRO_0000320048 | 2 – 304 | DCN1-like protein 3Add BLAST | 303 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineSequence analysis1 Publication | 1 |
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
EPDi | Q8IWE4 |
jPOSTi | Q8IWE4 |
MassIVEi | Q8IWE4 |
MaxQBi | Q8IWE4 |
PaxDbi | Q8IWE4 |
PeptideAtlasi | Q8IWE4 |
PRIDEi | Q8IWE4 |
ProteomicsDBi | 70849 |
PTM databases
iPTMneti | Q8IWE4 |
PhosphoSitePlusi | Q8IWE4 |
SwissPalmi | Q8IWE4 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000188215, Expressed in cardiac muscle of right atrium and 200 other tissues |
Genevisiblei | Q8IWE4, HS |
Organism-specific databases
HPAi | ENSG00000188215, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Part of a complex containing DCUN1D3, CUL3 and RBX1 (PubMed:19617556).
Interacts (via the DCUN1 domain) with the unneddylated cullins: interacts with CUL1, CUL2, CUL3, CUL4A, CUL4B and CUL5; these interactions promote the cullin neddylation and the identity of the cullin dictates the affinity of the interaction (PubMed:26906416, PubMed:23201271, PubMed:27542266).
Interacts preferentially with CUL3; this interaction triggers the relocalization of CUL3 to the cell membrane where CUL3 is neddylated (PubMed:19617556).
Interacts (via DCUN1 domain) with RBX1 (PubMed:26906416, PubMed:25349211). May also interact with regulators or subunits of cullin-RING ligases such as RNF7, ELOB and DDB1; these interactions are bridged by cullins (PubMed:26906416).
Interacts (via DCUN1 domain) with CAND1; this interaction is bridged by cullins and strongly inhibits cullin neddylation (PubMed:26906416, PubMed:27542266). These CAND-cullin-DCNL complexes can only be neddylated in the presence of a substrate adapter (PubMed:26906416).
Interacts (via DCUN1 domain) with the N-terminally acetylated form of UBE2M and UBE2F (PubMed:23201271, PubMed:25349211, PubMed:19617556).
5 PublicationsBinary interactionsi
Hide detailsQ8IWE4
With | #Exp. | IntAct |
---|---|---|
CUL3 [Q13618] | 2 | EBI-15794102,EBI-456129 |
CUL3 - isoform 1 [Q13618-1] | 3 | EBI-15794102,EBI-15794202 |
GO - Molecular functioni
- cullin family protein binding Source: UniProtKB
- ubiquitin conjugating enzyme binding Source: GO_Central
- ubiquitin-like protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 125841, 21 interactors |
DIPi | DIP-60769N |
ELMi | Q8IWE4 |
IntActi | Q8IWE4, 7 interactors |
STRINGi | 9606.ENSP00000319482 |
Miscellaneous databases
RNActi | Q8IWE4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8IWE4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 86 – 278 | DCUN1PROSITE-ProRule annotationAdd BLAST | 193 |
Domaini
Phylogenomic databases
eggNOGi | KOG3077, Eukaryota |
GeneTreei | ENSGT00940000154944 |
HOGENOMi | CLU_047042_2_0_1 |
InParanoidi | Q8IWE4 |
OMAi | DQWLHFL |
OrthoDBi | 1418097at2759 |
PhylomeDBi | Q8IWE4 |
TreeFami | TF313332 |
Family and domain databases
Gene3Di | 1.10.238.200, 1 hit |
InterProi | View protein in InterPro IPR014764, DCN-prot IPR042460, DCN1-like_PONY IPR005176, PONY_dom |
PANTHERi | PTHR12281, PTHR12281, 1 hit |
Pfami | View protein in Pfam PF03556, Cullin_binding, 1 hit |
PROSITEi | View protein in PROSITE PS51229, DCUN1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGQCVTKCKN PSSTLGSKNG DREPSNKSHS RRGAGHREEQ VPPCGKPGGD
60 70 80 90 100
ILVNGTKKAE AATEACQLPT SSGDAGRESK SNAEESSLQR LEELFRRYKD
110 120 130 140 150
EREDAILEEG MERFCNDLCV DPTEFRVLLL AWKFQAATMC KFTRKEFFDG
160 170 180 190 200
CKAISADSID GICARFPSLL TEAKQEDKFK DLYRFTFQFG LDSEEGQRSL
210 220 230 240 250
HREIAIALWK LVFTQNNPPV LDQWLNFLTE NPSGIKGISR DTWNMFLNFT
260 270 280 290 300
QVIGPDLSNY SEDEAWPSLF DTFVEWEMER RKREGEGRGA LSSGPEGLCP
EEQT
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_072689 | 2 | G → S in a cancer; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_072690 | 239 | S → F in a cancer; unknown pathological significance. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY364247 mRNA Translation: AAQ76806.1 AK123719 mRNA Translation: BAG53946.1 CH471228 Genomic DNA Translation: EAW66842.1 BC040442 mRNA Translation: AAH40442.1 |
CCDSi | CCDS10592.1 |
RefSeqi | NP_775746.1, NM_173475.3 |
Genome annotation databases
Ensembli | ENST00000324344; ENSP00000319482; ENSG00000188215 ENST00000563934; ENSP00000454762; ENSG00000188215 |
GeneIDi | 123879 |
KEGGi | hsa:123879 |
UCSCi | uc002dhz.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY364247 mRNA Translation: AAQ76806.1 AK123719 mRNA Translation: BAG53946.1 CH471228 Genomic DNA Translation: EAW66842.1 BC040442 mRNA Translation: AAH40442.1 |
CCDSi | CCDS10592.1 |
RefSeqi | NP_775746.1, NM_173475.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4GBA | X-ray | 2.40 | A/B | 86-304 | [»] | |
SMRi | Q8IWE4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125841, 21 interactors |
DIPi | DIP-60769N |
ELMi | Q8IWE4 |
IntActi | Q8IWE4, 7 interactors |
STRINGi | 9606.ENSP00000319482 |
Chemistry databases
ChEMBLi | CHEMBL4295894 |
PTM databases
iPTMneti | Q8IWE4 |
PhosphoSitePlusi | Q8IWE4 |
SwissPalmi | Q8IWE4 |
Genetic variation databases
BioMutai | DCUN1D3 |
DMDMi | 74728175 |
Proteomic databases
EPDi | Q8IWE4 |
jPOSTi | Q8IWE4 |
MassIVEi | Q8IWE4 |
MaxQBi | Q8IWE4 |
PaxDbi | Q8IWE4 |
PeptideAtlasi | Q8IWE4 |
PRIDEi | Q8IWE4 |
ProteomicsDBi | 70849 |
Protocols and materials databases
Antibodypediai | 25634, 65 antibodies |
Genome annotation databases
Ensembli | ENST00000324344; ENSP00000319482; ENSG00000188215 ENST00000563934; ENSP00000454762; ENSG00000188215 |
GeneIDi | 123879 |
KEGGi | hsa:123879 |
UCSCi | uc002dhz.4, human |
Organism-specific databases
CTDi | 123879 |
DisGeNETi | 123879 |
GeneCardsi | DCUN1D3 |
HGNCi | HGNC:28734, DCUN1D3 |
HPAi | ENSG00000188215, Tissue enhanced (skeletal) |
MIMi | 616167, gene |
neXtProti | NX_Q8IWE4 |
OpenTargetsi | ENSG00000188215 |
PharmGKBi | PA142672009 |
VEuPathDBi | HostDB:ENSG00000188215.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3077, Eukaryota |
GeneTreei | ENSGT00940000154944 |
HOGENOMi | CLU_047042_2_0_1 |
InParanoidi | Q8IWE4 |
OMAi | DQWLHFL |
OrthoDBi | 1418097at2759 |
PhylomeDBi | Q8IWE4 |
TreeFami | TF313332 |
Enzyme and pathway databases
PathwayCommonsi | Q8IWE4 |
Reactomei | R-HSA-8951664, Neddylation |
Miscellaneous databases
BioGRID-ORCSi | 123879, 16 hits in 1003 CRISPR screens |
GenomeRNAii | 123879 |
Pharosi | Q8IWE4, Tdark |
PROi | PR:Q8IWE4 |
RNActi | Q8IWE4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000188215, Expressed in cardiac muscle of right atrium and 200 other tissues |
Genevisiblei | Q8IWE4, HS |
Family and domain databases
Gene3Di | 1.10.238.200, 1 hit |
InterProi | View protein in InterPro IPR014764, DCN-prot IPR042460, DCN1-like_PONY IPR005176, PONY_dom |
PANTHERi | PTHR12281, PTHR12281, 1 hit |
Pfami | View protein in Pfam PF03556, Cullin_binding, 1 hit |
PROSITEi | View protein in PROSITE PS51229, DCUN1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCNL3_HUMAN | |
Accessioni | Q8IWE4Primary (citable) accession number: Q8IWE4 Secondary accession number(s): B3KVY4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references