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Protein

Inactive serine/threonine-protein kinase TEX14

Gene

TEX14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro (By similarity).By similarity

Caution

Ser-376 is present instead of the conserved Asp which is expected to be an active site residue.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei273ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi233 – 241ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: InterPro
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi1.A.87.2.7 the mechanosensitive calcium channel (mca) family

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive serine/threonine-protein kinase TEX14
Alternative name(s):
Protein kinase-like protein SgK307
Sugen kinase 307
Testis-expressed sequence 14
Testis-expressed sequence 14 protein
Gene namesi
Name:TEX14
Synonyms:SGK307
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000121101.15
HGNCiHGNC:11737 TEX14
MIMi605792 gene
neXtProtiNX_Q8IWB6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore

Pathology & Biotechi

Involvement in diseasei

Spermatogenic failure 23 (SPGF23)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn infertility disorder caused by spermatogenesis defects that result in non-obstructive azoospermia.
See also OMIM:617707

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi614 – 618SSPST → AAPAA in PBD-1; reduced ability to interact with PLK1. 1 Publication5
Mutagenesisi726 – 728STT → AAA in PBD-5; reduced ability to interact with PLK1. 1 Publication3

Organism-specific databases

DisGeNETi56155
MalaCardsiTEX14
MIMi617707 phenotype
OpenTargetsiENSG00000121101
PharmGKBiPA36454

Polymorphism and mutation databases

BioMutaiTEX14
DMDMi110816441

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002469951 – 1497Inactive serine/threonine-protein kinase TEX14Add BLAST1497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei175PhosphoserineBy similarity1
Modified residuei437Phosphoserine; by PLK1By similarity1
Modified residuei567PhosphoserineBy similarity1
Modified residuei665PhosphoserineBy similarity1
Modified residuei1106PhosphoserineBy similarity1
Modified residuei1404PhosphoserineBy similarity1
Modified residuei1459PhosphoserineBy similarity1
Modified residuei1496PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Thr residues by CDK1 during early phases of mitosis, promoting the interaction with PLK1 and recruitment to kinetochores. Phosphorylated on Ser-437 by PLK1 during late prometaphase promotes the rapid depletion from kinetochores and its subsequent degradation by the APC/C complex.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8IWB6
PeptideAtlasiQ8IWB6
PRIDEiQ8IWB6
ProteomicsDBi70835
70836 [Q8IWB6-2]
70837 [Q8IWB6-3]

PTM databases

iPTMnetiQ8IWB6
PhosphoSitePlusiQ8IWB6

Expressioni

Tissue specificityi

Expression restricted to testis.2 Publications

Gene expression databases

BgeeiENSG00000121101 Expressed in 102 organ(s), highest expression level in testis
CleanExiHS_TEX14
ExpressionAtlasiQ8IWB6 baseline and differential
GenevisibleiQ8IWB6 HS

Organism-specific databases

HPAiHPA030620
HPA077020

Interactioni

Subunit structurei

Interacts with KIF23 and RBM44. Interacts with CEP55; inhibiting interaction between CEP55 and PDCD6IP/ALIX and TSG101 (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121089, 3 interactors
ELMiQ8IWB6
IntActiQ8IWB6, 5 interactors
STRINGi9606.ENSP00000240361

Structurei

Secondary structure

11497
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8IWB6
SMRiQ8IWB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 54ANK 1Add BLAST28
Repeati55 – 84ANK 2Add BLAST30
Repeati88 – 117ANK 3Add BLAST30
Domaini227 – 518Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi795 – 801GPPX3Y7
Motifi852 – 860D-box9

Domaini

The protein kinase domain is predicted to be catalytically inactive.
The GPPX3Y motif mediates interaction with CEP55.

Sequence similaritiesi

Belongs to the protein kinase superfamily.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IHZA Eukaryota
ENOG410XT67 LUCA
GeneTreeiENSGT00390000015123
HOGENOMiHOG000059264
HOVERGENiHBG094035
InParanoidiQ8IWB6
KOiK17540
OMAiLEYMMES
OrthoDBiEOG091G0CDS
PhylomeDBiQ8IWB6
TreeFamiTF328704

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR039339 Tex14
PANTHERiPTHR23060 PTHR23060, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IWB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSRAVRLPVP CPVQLGTLRN DSLEAQLHEY VKQGNYVKVK KILKKGIYVD
60 70 80 90 100
AVNSLGQTAL FVAALLGLRK FVDVLVDYGS DPNHRCFDGS TPVHAAAFSG
110 120 130 140 150
NQWILSKLLD AGGDLRLHDE RGQNPKTWAL TAGKERSTQI VEFMQRCASH
160 170 180 190 200
MQAIIQGFSY DLLKKIDSPQ RLVYSPSWCG GLVQGNPNGS PNRLLKAGVI
210 220 230 240 250
SAQNIYSFGF GKAMPWFQFY LTGATQMAYL GSLPVIGEKE VIQADDEPTF
260 270 280 290 300
SFFSGPYMVM TNLVWNGSRV TVKELNLPTH PHCSRLRLAD LLIAEQEHSS
310 320 330 340 350
KLRHPYLLQL MAVCLSQDLE KTRLVYERIT IGTLFSVLHE RRSQFPVLHM
360 370 380 390 400
EVIVHLLLQI SDALRYLHFQ GFIHRSLSSY AVHIISPGEA RLTNLEYMLE
410 420 430 440 450
SEDRGVQRDL TRVPLPTQLY NWAAPEVILQ KAATVKSDIY SFSMIMQEIL
460 470 480 490 500
TDDIPWKGLD GSVVKKAVVS GNYLEADVRL PKPYYDIVKS GIHVKQKDRT
510 520 530 540 550
MNLQDIRYIL KNDLKDFTGA QRTQPTESPR VQRYGLHPDV NVYLGLTSEH
560 570 580 590 600
PRETPDMEII ELKEMGSQPH SPRVHSLFTE GTLDPQAPDP CLMARETQNQ
610 620 630 640 650
DAPCPAPFMA EEASSPSTGQ PSLCSFEINE IYSGCLILED DIEEPPGAAS
660 670 680 690 700
SLEADGPNQV DELKSMEEEL DKMEREACCF GSEDESSSKA ETEYSFDDWD
710 720 730 740 750
WQNGSLSSLS LPESTREAKS NLNNMSTTEE YLISKCVLDL KIMQTIMHEN
760 770 780 790 800
DDRLRNIEQI LDEVEMKQKE QEERMSLWAT SREFTNAYKL PLAVGPPSLN
810 820 830 840 850
YIPPVLQLSG GQKPDTSGNY PTLPRFPRML PTLCDPGKQN TDEQFQCTQG
860 870 880 890 900
AKDSLETSRI QNTSSQGRPR ESTAQAKATQ FNSALFTLSS HRQGPSASPS
910 920 930 940 950
CHWDSTRMSV EPVSSEIYNA ESRNKDDGKV HLKWKMEVKE MAKKAATGQL
960 970 980 990 1000
TVPPWHPQSS LTLESEAENE PDALLQPPIR SPENTDWQRV IEYHRENDEP
1010 1020 1030 1040 1050
RGNGKFDKTG NNDCDSDQHG RQPRLGSFTS IRHPSPRQKE QPEHSEAFQA
1060 1070 1080 1090 1100
SSDTLVAVEK SYSHQSMQST CSPESSEDIT DEFLTPDGEY FYSSTAQENL
1110 1120 1130 1140 1150
ALETSSPIEE DFEGIQGAFA QPQVSGEEKF QMRKILGKNA EILPRSQFQP
1160 1170 1180 1190 1200
VRSTEDEQEE TSKESPKELK EKDISLTDIQ DLSSISYEPD SSFKEASCKT
1210 1220 1230 1240 1250
PKINHAPTSV STPLSPGSVS SAASQYKDCL ESITFQVKTE FASCWNSQEF
1260 1270 1280 1290 1300
IQTLSDDFIS VRERAKKLDS LLTSSETPPS RLTGLKRLSS FIGAGSPSLV
1310 1320 1330 1340 1350
KACDSSPPHA TQRRSLPKVE AFSQHHIDEL PPPSQELLDD IELLKQQQGS
1360 1370 1380 1390 1400
STVLHENTAS DGGGTANDQR HLEEQETDSK KEDSSMLLSK ETEDLGEDTE
1410 1420 1430 1440 1450
RAHSTLDEDL ERWLQPPEES VELQDLPKGS ERETNIKDQK VGEEKRKRED
1460 1470 1480 1490
SITPERRKSE GVLGTSEEDE LKSCFWKRLG WSESSRIIVL DQSDLSD
Length:1,497
Mass (Da):167,901
Last modified:July 25, 2006 - v2
Checksum:iD7DC446A1A18FC4D
GO
Isoform 2 (identifier: Q8IWB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-218: Missing.

Show »
Length:1,491
Mass (Da):167,140
Checksum:iAC936A9224CC0DA9
GO
Isoform 3 (identifier: Q8IWB6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-218: Missing.
     1064-1103: Missing.

Show »
Length:1,451
Mass (Da):162,669
Checksum:i10F8DE7EF828BC3E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3QKU8J3QKU8_HUMAN
Inactive serine/threonine-protein k...
TEX14
152Annotation score:
J3QLK3J3QLK3_HUMAN
Inactive serine/threonine-protein k...
TEX14
66Annotation score:

Sequence cautioni

The sequence AAK31980 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1326H → R in AAK31980 (PubMed:11279525).Curated1
Sequence conflicti1387L → P in CAD38856 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04138888D → G1 PublicationCorresponds to variant dbSNP:rs56292204Ensembl.1
Natural variantiVAR_041389321K → E in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041390443S → C1 Publication1
Natural variantiVAR_041391559I → M1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019868213 – 218Missing in isoform 2 and isoform 3. 2 Publications6
Alternative sequenceiVSP_0198691064 – 1103Missing in isoform 3. 2 PublicationsAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL834143 mRNA Translation: CAD38856.2
AC005666 Genomic DNA No translation available.
BC040526 mRNA Translation: AAH40526.1
AF285601 mRNA Translation: AAK31980.1 Different initiation.
BK000998 mRNA Translation: DAA01359.1
CCDSiCCDS32692.1 [Q8IWB6-3]
CCDS32693.1 [Q8IWB6-2]
CCDS56042.1 [Q8IWB6-1]
RefSeqiNP_001188386.1, NM_001201457.1 [Q8IWB6-1]
NP_112562.3, NM_031272.4 [Q8IWB6-3]
NP_938207.2, NM_198393.3 [Q8IWB6-2]
UniGeneiHs.390221

Genome annotation databases

EnsembliENST00000240361; ENSP00000240361; ENSG00000121101 [Q8IWB6-1]
ENST00000349033; ENSP00000268910; ENSG00000121101 [Q8IWB6-3]
ENST00000389934; ENSP00000374584; ENSG00000121101 [Q8IWB6-2]
GeneIDi56155
KEGGihsa:56155
UCSCiuc002iwr.3 human [Q8IWB6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL834143 mRNA Translation: CAD38856.2
AC005666 Genomic DNA No translation available.
BC040526 mRNA Translation: AAH40526.1
AF285601 mRNA Translation: AAK31980.1 Different initiation.
BK000998 mRNA Translation: DAA01359.1
CCDSiCCDS32692.1 [Q8IWB6-3]
CCDS32693.1 [Q8IWB6-2]
CCDS56042.1 [Q8IWB6-1]
RefSeqiNP_001188386.1, NM_001201457.1 [Q8IWB6-1]
NP_112562.3, NM_031272.4 [Q8IWB6-3]
NP_938207.2, NM_198393.3 [Q8IWB6-2]
UniGeneiHs.390221

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WUTX-ray2.30C/F/I/L792-804[»]
3WUUX-ray2.90C/F/I/L791-805[»]
ProteinModelPortaliQ8IWB6
SMRiQ8IWB6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121089, 3 interactors
ELMiQ8IWB6
IntActiQ8IWB6, 5 interactors
STRINGi9606.ENSP00000240361

Protein family/group databases

TCDBi1.A.87.2.7 the mechanosensitive calcium channel (mca) family

PTM databases

iPTMnetiQ8IWB6
PhosphoSitePlusiQ8IWB6

Polymorphism and mutation databases

BioMutaiTEX14
DMDMi110816441

Proteomic databases

PaxDbiQ8IWB6
PeptideAtlasiQ8IWB6
PRIDEiQ8IWB6
ProteomicsDBi70835
70836 [Q8IWB6-2]
70837 [Q8IWB6-3]

Protocols and materials databases

DNASUi56155
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000240361; ENSP00000240361; ENSG00000121101 [Q8IWB6-1]
ENST00000349033; ENSP00000268910; ENSG00000121101 [Q8IWB6-3]
ENST00000389934; ENSP00000374584; ENSG00000121101 [Q8IWB6-2]
GeneIDi56155
KEGGihsa:56155
UCSCiuc002iwr.3 human [Q8IWB6-1]

Organism-specific databases

CTDi56155
DisGeNETi56155
EuPathDBiHostDB:ENSG00000121101.15
GeneCardsiTEX14
HGNCiHGNC:11737 TEX14
HPAiHPA030620
HPA077020
MalaCardsiTEX14
MIMi605792 gene
617707 phenotype
neXtProtiNX_Q8IWB6
OpenTargetsiENSG00000121101
PharmGKBiPA36454
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHZA Eukaryota
ENOG410XT67 LUCA
GeneTreeiENSGT00390000015123
HOGENOMiHOG000059264
HOVERGENiHBG094035
InParanoidiQ8IWB6
KOiK17540
OMAiLEYMMES
OrthoDBiEOG091G0CDS
PhylomeDBiQ8IWB6
TreeFamiTF328704

Miscellaneous databases

ChiTaRSiTEX14 human
GenomeRNAii56155
PROiPR:Q8IWB6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000121101 Expressed in 102 organ(s), highest expression level in testis
CleanExiHS_TEX14
ExpressionAtlasiQ8IWB6 baseline and differential
GenevisibleiQ8IWB6 HS

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR039339 Tex14
PANTHERiPTHR23060 PTHR23060, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTEX14_HUMAN
AccessioniPrimary (citable) accession number: Q8IWB6
Secondary accession number(s): A6NH19
, Q7RTP3, Q8ND97, Q9BXT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: September 12, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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