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Entry version 169 (12 Aug 2020)
Sequence version 3 (03 Sep 2014)
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Protein

Kinase suppressor of Ras 1

Gene

KSR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).By similarity1 Publication

Caution

Although it belongs to the protein kinase superfamily, the ATP-binding motif VAIK has an arginine instead of a lysine residue suggesting that KSR1 can not bind ATP and therefore lacks protein kinase activity. However, KSR1 is capable of binding ATP (PubMed:29433126). Has protein kinase activity towards MAP2K1 in the presence of RAF1/c-RAF in vitro (By similarity).By similarityCurated1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi348Zinc 1By similarity1
Metal bindingi360Zinc 2By similarity1
Metal bindingi363Zinc 2By similarity1
Metal bindingi373Zinc 1By similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi381Zinc 2By similarity1
Metal bindingi384Zinc 2By similarity1
Metal bindingi391Zinc 1By similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei733Proton acceptorPROSITE-ProRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei735ATPBy similarity1
Binding sitei750ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri347 – 391Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST45
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi619 – 627ATPPROSITE-ProRule annotation9

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

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PathwayCommonsi
Q8IVT5

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-5673000, RAF activation
R-HSA-5674135, MAP2K and MAPK activation
R-HSA-5675221, Negative regulation of MAPK pathway
R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants
R-HSA-6802948, Signaling by high-kinase activity BRAF mutants
R-HSA-6802952, Signaling by BRAF and RAF fusions
R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-9649948, Signaling downstream of RAS mutants

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...
SignaLinki
Q8IVT5

SIGNOR Signaling Network Open Resource

More...
SIGNORi
Q8IVT5

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Kinase suppressor of Ras 1 (EC:2.7.11.1By similarity)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:KSR1
Synonyms:KSR
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
HostDB:ENSG00000141068.13

Human Gene Nomenclature Database

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HGNCi
HGNC:6465, KSR1

Online Mendelian Inheritance in Man (OMIM)

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MIMi
601132, gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_Q8IVT5

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Endoplasmic reticulum, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi47L → D: Reduces interaction with BRAF and MAP2K1 and thus phosphorylation of MAP2K1. 1 Publication1
Mutagenesisi62C → D: Reduces interaction with BRAF and MAP2K1 and thus phosphorylation of MAP2K1. 1 Publication1
Mutagenesisi72E → K: Loss of interaction with BRAF and MAP2K1 and severe decrease in MAP2K1 phosphorylation levels. 1 Publication1
Mutagenesisi602 – 604YLQ → DDE: No effect on MAP2K1- or MAP2K2-mediated-BRAF-KSR1 dimerization and BRAF activation. 1 Publication3
Mutagenesisi637A → F: No effect on MAP2K1 or MAP2K2 mediated-BRAF-KSR1 dimerization and BRAF activation. 1 Publication1
Mutagenesisi639R → M: No effect on MAP2K1 mediated-BRAF-KSR1 dimerization and BRAF activation. 1 Publication1
Mutagenesisi665R → H: Loss of MAP2K1 mediated-BRAF-KSR1 dimerization. 1 Publication1
Mutagenesisi831W → R: Loss of the interaction with MAP2K1 resulting in loss of BRAF-KSR1 dimerization and BRAF activation. No effect on ATP binding or interaction with HSP90AA1, 14-3-3 and CDC37. 1 Publication1

Organism-specific databases

DisGeNET

More...
DisGeNETi
8844

Open Targets

More...
OpenTargetsi
ENSG00000141068

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA30254

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q8IVT5, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...
ChEMBLi
CHEMBL1938215

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
KSR1

Domain mapping of disease mutations (DMDM)

More...
DMDMi
56749095

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000862291 – 923Kinase suppressor of Ras 1Add BLAST923

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei270PhosphothreonineCombined sources1
Modified residuei274PhosphothreonineCombined sources1
Modified residuei311PhosphoserineCombined sources1
Modified residuei334PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Modified residuei406PhosphoserineCombined sources1
Modified residuei425PhosphothreonineCombined sources1
Modified residuei569PhosphoserineCombined sources1
Modified residuei888PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q8IVT5

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q8IVT5

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q8IVT5

MaxQB - The MaxQuant DataBase

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MaxQBi
Q8IVT5

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q8IVT5

PeptideAtlas

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PeptideAtlasi
Q8IVT5

PRoteomics IDEntifications database

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PRIDEi
Q8IVT5

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
31779
43732
70766 [Q8IVT5-1]
70767 [Q8IVT5-2]
70768 [Q8IVT5-3]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q8IVT5

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q8IVT5

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000141068, Expressed in body of pancreas and 211 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q8IVT5, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q8IVT5, HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000141068, Tissue enhanced (pancreas)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer (PubMed:29433126). Heterodimerizes (via N-terminus) with BRAF (via N-terminus) in a MAP2K1/MEK1 or MAP2K2/MEK2-dependent manner (PubMed:29433126).

Interacts with MAP2K1/MEK1 and MAP2K2/MEK2 (PubMed:10409742, PubMed:29433126). Binding to MAP2K1/MEK1 releases the intramolecular inhibitory interaction between KSR1 N-terminus and kinase domains which is required for the subsequent RSK1 dimerization with BRAF (PubMed:29433126).

Identified in a complex with AKAP13, MAP2K1 and BRAF (By similarity).

Interacts with AKAP13 and BRAF (PubMed:21102438).

Interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity).

Interacts with 14-3-3 proteins including YWHAB (By similarity).

Interacts with HSP90AA1/HSP90, YWHAE/14-3-3 and CDC37 (PubMed:10409742, PubMed:29433126). The binding of 14-3-3 proteins to phosphorylated KSR1 prevents the membrane localization (By similarity).

Interacts with MARK3 (By similarity).

Interacts with PPP2R1A and PPP2CA (By similarity).

Interacts with isoform 1 of VRK2 (PubMed:20679487).

By similarity4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
114371, 108 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q8IVT5

Database of interacting proteins

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DIPi
DIP-32619N

Protein interaction database and analysis system

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IntActi
Q8IVT5, 358 interactors

Molecular INTeraction database

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MINTi
Q8IVT5

STRING: functional protein association networks

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STRINGi
9606.ENSP00000381958

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
Q8IVT5, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1923
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q8IVT5

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini613 – 883Protein kinasePROSITE-ProRule annotationAdd BLAST271

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 172Mediates association with membranesBy similarityAdd BLAST172

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi17 – 21Poly-Gly5
Compositional biasi289 – 292Poly-Pro4

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation (PubMed:10409742).Sequence analysis1 Publication
The N-terminal region mediates interaction with BRAF (PubMed:29433126). Also mediates membrane localization (By similarity).By similarity1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri347 – 391Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST45

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG0193, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00940000156066

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q8IVT5

KEGG Orthology (KO)

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KOi
K14958

Database of Orthologous Groups

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OrthoDBi
281487at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q8IVT5

TreeFam database of animal gene trees

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TreeFami
TF317006

Family and domain databases

Conserved Domains Database

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CDDi
cd00029, C1, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.10.150.50, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR011009, Kinase-like_dom_sf
IPR025561, KSR_SAM-like_dom
IPR002219, PE/DAG-bd
IPR000719, Prot_kinase_dom
IPR013761, SAM/pointed_sf
IPR001245, Ser-Thr/Tyr_kinase_cat_dom
IPR008271, Ser/Thr_kinase_AS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07714, Pkinase_Tyr, 1 hit
PF13543, SAM_KSR1, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00109, C1, 1 hit
SM00220, S_TKc, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF56112, SSF56112, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50011, PROTEIN_KINASE_DOM, 1 hit
PS00108, PROTEIN_KINASE_ST, 1 hit
PS00479, ZF_DAG_PE_1, 1 hit
PS50081, ZF_DAG_PE_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IVT5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDRAALRAAA MGEKKEGGGG GDAAAAEGGA GAAASRALQQ CGQLQKLIDI
60 70 80 90 100
SIGSLRGLRT KCAVSNDLTQ QEIRTLEAKL VRYICKQRQC KLSVAPGERT
110 120 130 140 150
PELNSYPRFS DWLYTFNVRP EVVQEIPRDL TLDALLEMNE AKVKETLRRC
160 170 180 190 200
GASGDECGRL QYALTCLRKV TGLGGEHKED SSWSSLDARR ESGSGPSTDT
210 220 230 240 250
LSAASLPWPP GSSQLGRAGN SAQGPRSISV SALPASDSPT PSFSEGLSDT
260 270 280 290 300
CIPLHASGRL TPRALHSFIT PPTTPQLRRH TKLKPPRTPP PPSRKVFQLL
310 320 330 340 350
PSFPTLTRSK SHESQLGNRI DDVSSMRFDL SHGSPQMVRR DIGLSVTHRF
360 370 380 390 400
STKSWLSQVC HVCQKSMIFG VKCKHCRLKC HNKCTKEAPA CRISFLPLTR
410 420 430 440 450
LRRTESVPSD INNPVDRAAE PHFGTLPKAL TKKEHPPAMN HLDSSSNPSS
460 470 480 490 500
TTSSTPSSPA PFPTSSNPSS ATTPPNPSPG QRDSRFNFPA AYFIHHRQQF
510 520 530 540 550
IFPVPSAGHC WKCLLIAESL KENAFNISAF AHAAPLPEAA DGTRLDDQPK
560 570 580 590 600
ADVLEAHEAE AEEPEAGKSE AEDDEDEVDD LPSSRRPWRG PISRKASQTS
610 620 630 640 650
VYLQEWDIPF EQVELGEPIG QGRWGRVHRG RWHGEVAIRL LEMDGHNQDH
660 670 680 690 700
LKLFKKEVMN YRQTRHENVV LFMGACMNPP HLAIITSFCK GRTLHSFVRD
710 720 730 740 750
PKTSLDINKT RQIAQEIIKG MGYLHAKGIV HKDLKSKNVF YDNGKVVITD
760 770 780 790 800
FGLFGISGVV REGRRENQLK LSHDWLCYLA PEIVREMTPG KDEDQLPFSK
810 820 830 840 850
AADVYAFGTV WYELQARDWP LKNQAAEASI WQIGSGEGMK RVLTSVSLGK
860 870 880 890 900
EVSEILSACW AFDLQERPSF SLLMDMLEKL PKLNRRLSHP GHFWKSADIN
910 920
SSKVVPRFER FGLGVLESSN PKM
Length:923
Mass (Da):102,160
Last modified:September 3, 2014 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i61BF0F1598349540
GO
Isoform 2 (identifier: Q8IVT5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-526: VPSAGHCWKCLLIAESLKENAFN → D
     898-923: DINSSKVVPRFERFGLGVLESSNPKM → EL

Show »
Length:877
Mass (Da):97,115
Checksum:iB316183895D9A4BD
GO
Isoform 3 (identifier: Q8IVT5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-137: Missing.

Show »
Length:786
Mass (Da):87,344
Checksum:i7BDD722CFE4B87BA
GO
Isoform 4 (identifier: Q8IVT5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-137: Missing.
     898-923: DINSSKVVPRFERFGLGVLESSNPKM → EL

Show »
Length:762
Mass (Da):84,697
Checksum:i3034D20D01F33E66
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MQW1A0A0A0MQW1_HUMAN
Kinase suppressor of Ras 1
KSR1
846Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A2R8Y4X0A0A2R8Y4X0_HUMAN
Kinase suppressor of Ras 1
KSR1
829Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A2R8Y5H9A0A2R8Y5H9_HUMAN
Kinase suppressor of Ras 1
KSR1
928Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A8MY87A8MY87_HUMAN
Kinase suppressor of Ras 1
KSR1
612Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QR75J3QR75_HUMAN
Kinase suppressor of Ras 1
KSR1
155Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QLI1J3QLI1_HUMAN
Kinase suppressor of Ras 1
KSR1
98Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QQL4J3QQL4_HUMAN
Kinase suppressor of Ras 1
KSR1
139Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QSG8J3QSG8_HUMAN
Kinase suppressor of Ras 1
KSR1
113Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti243 – 265FSEGL…TPRAL → EFRHTSALTQHTAHTQHTSA HTQ in AAH42106 (PubMed:8521512).CuratedAdd BLAST23

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_040658227S → P1 Publication1
Natural variantiVAR_046048359V → A1 Publication1
Natural variantiVAR_046049663Q → H1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0556551 – 137Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST137
Alternative sequenceiVSP_055656504 – 526VPSAG…ENAFN → D in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_055657898 – 923DINSS…SNPKM → EL in isoform 2 and isoform 4. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AC069366 Genomic DNA No translation available.
AC015688 Genomic DNA No translation available.
BC042106 mRNA Translation: AAH42106.1
U43586 mRNA Translation: AAC50354.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS58532.1 [Q8IVT5-4]

NCBI Reference Sequences

More...
RefSeqi
NP_055053.1, NM_014238.1 [Q8IVT5-4]
XP_011523731.1, XM_011525429.2 [Q8IVT5-1]
XP_016880760.1, XM_017025271.1 [Q8IVT5-3]
XP_016880761.1, XM_017025272.1 [Q8IVT5-3]
XP_016880762.1, XM_017025273.1 [Q8IVT5-3]
XP_016880763.1, XM_017025274.1 [Q8IVT5-3]
XP_016880764.1, XM_017025275.1 [Q8IVT5-3]
XP_016880765.1, XM_017025276.1 [Q8IVT5-3]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000398988; ENSP00000381958; ENSG00000141068 [Q8IVT5-4]
ENST00000509603; ENSP00000438795; ENSG00000141068 [Q8IVT5-4]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
8844

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:8844

UCSC genome browser

More...
UCSCi
uc002gzo.2, human [Q8IVT5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC069366 Genomic DNA No translation available.
AC015688 Genomic DNA No translation available.
BC042106 mRNA Translation: AAH42106.1
U43586 mRNA Translation: AAC50354.1
CCDSiCCDS58532.1 [Q8IVT5-4]
RefSeqiNP_055053.1, NM_014238.1 [Q8IVT5-4]
XP_011523731.1, XM_011525429.2 [Q8IVT5-1]
XP_016880760.1, XM_017025271.1 [Q8IVT5-3]
XP_016880761.1, XM_017025272.1 [Q8IVT5-3]
XP_016880762.1, XM_017025273.1 [Q8IVT5-3]
XP_016880763.1, XM_017025274.1 [Q8IVT5-3]
XP_016880764.1, XM_017025275.1 [Q8IVT5-3]
XP_016880765.1, XM_017025276.1 [Q8IVT5-3]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VYKX-ray1.75A/C27-172[»]
SMRiQ8IVT5
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi114371, 108 interactors
CORUMiQ8IVT5
DIPiDIP-32619N
IntActiQ8IVT5, 358 interactors
MINTiQ8IVT5
STRINGi9606.ENSP00000381958

Chemistry databases

ChEMBLiCHEMBL1938215

PTM databases

iPTMnetiQ8IVT5
PhosphoSitePlusiQ8IVT5

Polymorphism and mutation databases

BioMutaiKSR1
DMDMi56749095

Proteomic databases

EPDiQ8IVT5
jPOSTiQ8IVT5
MassIVEiQ8IVT5
MaxQBiQ8IVT5
PaxDbiQ8IVT5
PeptideAtlasiQ8IVT5
PRIDEiQ8IVT5
ProteomicsDBi31779
43732
70766 [Q8IVT5-1]
70767 [Q8IVT5-2]
70768 [Q8IVT5-3]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
2110, 410 antibodies

The DNASU plasmid repository

More...
DNASUi
8844

Genome annotation databases

EnsembliENST00000398988; ENSP00000381958; ENSG00000141068 [Q8IVT5-4]
ENST00000509603; ENSP00000438795; ENSG00000141068 [Q8IVT5-4]
GeneIDi8844
KEGGihsa:8844
UCSCiuc002gzo.2, human [Q8IVT5-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
8844
DisGeNETi8844
EuPathDBiHostDB:ENSG00000141068.13

GeneCards: human genes, protein and diseases

More...
GeneCardsi
KSR1
HGNCiHGNC:6465, KSR1
HPAiENSG00000141068, Tissue enhanced (pancreas)
MIMi601132, gene
neXtProtiNX_Q8IVT5
OpenTargetsiENSG00000141068
PharmGKBiPA30254

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG0193, Eukaryota
GeneTreeiENSGT00940000156066
InParanoidiQ8IVT5
KOiK14958
OrthoDBi281487at2759
PhylomeDBiQ8IVT5
TreeFamiTF317006

Enzyme and pathway databases

PathwayCommonsiQ8IVT5
ReactomeiR-HSA-5673000, RAF activation
R-HSA-5674135, MAP2K and MAPK activation
R-HSA-5675221, Negative regulation of MAPK pathway
R-HSA-6802946, Signaling by moderate kinase activity BRAF mutants
R-HSA-6802948, Signaling by high-kinase activity BRAF mutants
R-HSA-6802952, Signaling by BRAF and RAF fusions
R-HSA-6802955, Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-9649948, Signaling downstream of RAS mutants
SignaLinkiQ8IVT5
SIGNORiQ8IVT5

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
8844, 7 hits in 899 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
KSR1, human

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
KSR1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
8844
PharosiQ8IVT5, Tbio

Protein Ontology

More...
PROi
PR:Q8IVT5
RNActiQ8IVT5, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000141068, Expressed in body of pancreas and 211 other tissues
ExpressionAtlasiQ8IVT5, baseline and differential
GenevisibleiQ8IVT5, HS

Family and domain databases

CDDicd00029, C1, 1 hit
Gene3Di1.10.150.50, 1 hit
InterProiView protein in InterPro
IPR011009, Kinase-like_dom_sf
IPR025561, KSR_SAM-like_dom
IPR002219, PE/DAG-bd
IPR000719, Prot_kinase_dom
IPR013761, SAM/pointed_sf
IPR001245, Ser-Thr/Tyr_kinase_cat_dom
IPR008271, Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF07714, Pkinase_Tyr, 1 hit
PF13543, SAM_KSR1, 1 hit
SMARTiView protein in SMART
SM00109, C1, 1 hit
SM00220, S_TKc, 1 hit
SUPFAMiSSF56112, SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011, PROTEIN_KINASE_DOM, 1 hit
PS00108, PROTEIN_KINASE_ST, 1 hit
PS00479, ZF_DAG_PE_1, 1 hit
PS50081, ZF_DAG_PE_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiKSR1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8IVT5
Secondary accession number(s): F8WEA9, H7BYU0, Q13476
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: September 3, 2014
Last modified: August 12, 2020
This is version 169 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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