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UniProtKB - Q8IVL8 (CBPO_HUMAN)
Protein
Carboxypeptidase O
Gene
CPO
Organism
Homo sapiens (Human)
Status
Functioni
Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.
1 PublicationCofactori
Zn2+By similarity
Activity regulationi
Strongly inhibited by potato carboxypeptidase inhibitor, and the chelating agents EDTA and 1,10-phenanthroline. Also inhibited by compounds with multiple carboxylic acid groups such as citrate and succinate, and to a lesser exent the amino acids aspartate and glutamate. Not significantly inhibited by benzylsuccinic acid.1 Publication
Kineticsi
- KM=325 µM for 3-(2-furyl)acryloyl-Glu-Glu (at pH 7.5)1 Publication
- KM=284 µM for 3-(2-furyl)acryloyl-Phe-Phe (at pH 7.5)1 Publication
- KM=549 µM for 3-(2-furyl)acryloyl-Phe-Ala (at pH 7.5)1 Publication
- KM=614 µM for 3-(2-furyl)acryloyl-Lys-Ala (at pH 7.5)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 108 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 111 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 236 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 310 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- metallocarboxypeptidase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- proteolysis Source: GO_Central
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8IVL8 |
Protein family/group databases
MEROPSi | M14.021 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CPOImported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21011, CPO |
MIMi | 609563, gene |
neXtProti | NX_Q8IVL8 |
VEuPathDBi | HostDB:ENSG00000144410 |
Subcellular locationi
Plasma membrane
- Apical cell membrane 1 Publication; GPI-anchor 1 Publication
Extracellular region or secreted
- extracellular space Source: GO_Central
Plasma Membrane
- anchored component of plasma membrane Source: UniProtKB
- apical plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 130749 |
OpenTargetsi | ENSG00000144410 |
PharmGKBi | PA164741367 |
Miscellaneous databases
Pharosi | Q8IVL8, Tdark |
Genetic variation databases
BioMutai | CPO |
DMDMi | 74723635 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 1 PublicationAdd BLAST | 20 | |
ChainiPRO_0000252401 | 21 – 352 | Carboxypeptidase OCuratedAdd BLAST | 332 | |
PropeptideiPRO_0000437881 | 353 – 374 | Removed in mature formCuratedAdd BLAST | 22 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 132 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 174 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 187 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 251 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 352 | GPI-anchor amidated aspartateSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Glycoprotein, GPI-anchor, LipoproteinProteomic databases
MassIVEi | Q8IVL8 |
PaxDbi | Q8IVL8 |
PeptideAtlasi | Q8IVL8 |
PRIDEi | Q8IVL8 |
ProteomicsDBi | 70740 |
PTM databases
GlyGeni | Q8IVL8, 4 sites |
iPTMneti | Q8IVL8 |
PhosphoSitePlusi | Q8IVL8 |
Expressioni
Tissue specificityi
Detected in enterocytes of the ileum.1 Publication
Gene expression databases
Bgeei | ENSG00000144410, Expressed in small intestine Peyer's patch and 109 other tissues |
Genevisiblei | Q8IVL8, HS |
Organism-specific databases
HPAi | ENSG00000144410, Tissue enriched (intestine) |
Interactioni
Protein-protein interaction databases
STRINGi | 9606.ENSP00000272852 |
Miscellaneous databases
RNActi | Q8IVL8, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8IVL8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M14 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2650, Eukaryota |
GeneTreei | ENSGT00940000161508 |
HOGENOMi | CLU_019326_4_1_1 |
InParanoidi | Q8IVL8 |
OMAi | TKKIIWM |
OrthoDBi | 524270at2759 |
PhylomeDBi | Q8IVL8 |
TreeFami | TF317197 |
Family and domain databases
CDDi | cd06247, M14_CPO, 1 hit |
InterProi | View protein in InterPro IPR033850, Carboxypeptidase_O IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF00246, Peptidase_M14, 1 hit |
PRINTSi | PR00765, CRBOXYPTASEA |
SMARTi | View protein in SMART SM00631, Zn_pept, 1 hit |
PROSITEi | View protein in PROSITE PS00132, CARBOXYPEPT_ZN_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8IVL8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPLLETLYL LGMLVPGGLG YDRSLAQHRQ EIVDKSVSPW SLETYSYNIY
60 70 80 90 100
HPMGEIYEWM REISEKYKEV VTQHFLGVTY ETHPMYYLKI SQPSGNPKKI
110 120 130 140 150
IWMDCGIHAR EWIAPAFCQW FVKEILQNHK DNSSIRKLLR NLDFYVLPVL
160 170 180 190 200
NIDGYIYTWT TDRLWRKSRS PHNNGTCFGT DLNRNFNASW CSIGASRNCQ
210 220 230 240 250
DQTFCGTGPV SEPETKAVAS FIESKKDDIL CFLTMHSYGQ LILTPYGYTK
260 270 280 290 300
NKSSNHPEMI QVGQKAANAL KAKYGTNYRV GSSADILYAS SGSSRDWARD
310 320 330 340 350
IGIPFSYTFE LRDSGTYGFV LPEAQIQPTC EETMEAVLSV LDDVYAKHWH
360 370
SDSAGRVTSA TMLLGLLVSC MSLL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027850 | 85 | M → I1 PublicationCorresponds to variant dbSNP:rs13420911Ensembl. | 1 | |
Natural variantiVAR_027851 | 134 | S → R1 PublicationCorresponds to variant dbSNP:rs11903403Ensembl. | 1 | |
Natural variantiVAR_036012 | 273 | K → N in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs896000260Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ422118 mRNA Translation: CAD19478.1 AC019052 Genomic DNA Translation: AAX93277.1 BC112076 mRNA Translation: AAI12077.1 BC112078 mRNA Translation: AAI12079.1 BK000189 mRNA Translation: DAA00036.1 |
CCDSi | CCDS2372.1 |
RefSeqi | NP_775100.1, NM_173077.2 XP_016858861.1, XM_017003372.1 |
Genome annotation databases
Ensembli | ENST00000272852; ENSP00000272852; ENSG00000144410 |
GeneIDi | 130749 |
KEGGi | hsa:130749 |
MANE-Selecti | ENST00000272852.4; ENSP00000272852.2; NM_173077.3; NP_775100.1 |
UCSCi | uc002vby.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ422118 mRNA Translation: CAD19478.1 AC019052 Genomic DNA Translation: AAX93277.1 BC112076 mRNA Translation: AAI12077.1 BC112078 mRNA Translation: AAI12079.1 BK000189 mRNA Translation: DAA00036.1 |
CCDSi | CCDS2372.1 |
RefSeqi | NP_775100.1, NM_173077.2 XP_016858861.1, XM_017003372.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MRV | X-ray | 1.85 | A/B | 21-349 | [»] | |
SMRi | Q8IVL8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 9606.ENSP00000272852 |
Protein family/group databases
MEROPSi | M14.021 |
PTM databases
GlyGeni | Q8IVL8, 4 sites |
iPTMneti | Q8IVL8 |
PhosphoSitePlusi | Q8IVL8 |
Genetic variation databases
BioMutai | CPO |
DMDMi | 74723635 |
Proteomic databases
MassIVEi | Q8IVL8 |
PaxDbi | Q8IVL8 |
PeptideAtlasi | Q8IVL8 |
PRIDEi | Q8IVL8 |
ProteomicsDBi | 70740 |
Protocols and materials databases
Antibodypediai | 52118, 181 antibodies from 21 providers |
DNASUi | 130749 |
Genome annotation databases
Ensembli | ENST00000272852; ENSP00000272852; ENSG00000144410 |
GeneIDi | 130749 |
KEGGi | hsa:130749 |
MANE-Selecti | ENST00000272852.4; ENSP00000272852.2; NM_173077.3; NP_775100.1 |
UCSCi | uc002vby.2, human |
Organism-specific databases
CTDi | 130749 |
DisGeNETi | 130749 |
GeneCardsi | CPO |
HGNCi | HGNC:21011, CPO |
HPAi | ENSG00000144410, Tissue enriched (intestine) |
MIMi | 609563, gene |
neXtProti | NX_Q8IVL8 |
OpenTargetsi | ENSG00000144410 |
PharmGKBi | PA164741367 |
VEuPathDBi | HostDB:ENSG00000144410 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2650, Eukaryota |
GeneTreei | ENSGT00940000161508 |
HOGENOMi | CLU_019326_4_1_1 |
InParanoidi | Q8IVL8 |
OMAi | TKKIIWM |
OrthoDBi | 524270at2759 |
PhylomeDBi | Q8IVL8 |
TreeFami | TF317197 |
Enzyme and pathway databases
PathwayCommonsi | Q8IVL8 |
Miscellaneous databases
BioGRID-ORCSi | 130749, 5 hits in 1038 CRISPR screens |
ChiTaRSi | CPO, human |
GenomeRNAii | 130749 |
Pharosi | Q8IVL8, Tdark |
PROi | PR:Q8IVL8 |
RNActi | Q8IVL8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000144410, Expressed in small intestine Peyer's patch and 109 other tissues |
Genevisiblei | Q8IVL8, HS |
Family and domain databases
CDDi | cd06247, M14_CPO, 1 hit |
InterProi | View protein in InterPro IPR033850, Carboxypeptidase_O IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF00246, Peptidase_M14, 1 hit |
PRINTSi | PR00765, CRBOXYPTASEA |
SMARTi | View protein in SMART SM00631, Zn_pept, 1 hit |
PROSITEi | View protein in PROSITE PS00132, CARBOXYPEPT_ZN_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CBPO_HUMAN | |
Accessioni | Q8IVL8Primary (citable) accession number: Q8IVL8 Secondary accession number(s): Q2M277, Q7RTW7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families