PSTK

Functionⁱ

Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis.By similarity

Catalytic activityⁱ

ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec).

Cofactorⁱ

Mg²⁺By similarity

Pathwayⁱ: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

L-seryl-tRNA(Sec) kinase (PSTK)
O-phosphoseryl-tRNA(Sec) selenium transferase, O-phosphoseryl-tRNA(Sec) selenium transferase (SEPSECS), O-phosphoseryl-tRNA(Sec) selenium transferase (SEPSECS)

This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	25 – 32	ATPSequence analysis		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
kinase activity Source: UniProtKB-KW
tRNA binding Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

selenocysteine incorporation Source: Ensembl
selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Biological process	Protein biosynthesis
Ligand	ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-2408557 Selenocysteine synthesis
UniPathwayⁱ	UPA00906; UER00897

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: L-seryl-tRNA(Sec) kinase (EC:2.7.1.164) Alternative name(s): O-phosphoseryl-tRNA(Sec) kinase
Gene namesⁱ	Name:PSTK Synonyms:C10orf89
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 10

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000179988.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:28578 PSTK
MIMⁱ	611310 gene
neXtProtⁱ	NX_Q8IV42

Pathology & Biotechⁱ

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000179988
PharmGKBⁱ	PA162400268

Polymorphism and mutation databases

BioMutaⁱ	PSTK
DMDMⁱ	116242735

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000097080	1 – 348	L-seryl-tRNA(Sec) kinaseAdd BLAST		348

Proteomic databases

PaxDbⁱ	Q8IV42
PeptideAtlas More...PeptideAtlasⁱ	Q8IV42
PRIDEⁱ	Q8IV42
ProteomicsDBⁱ	70656 70657 [Q8IV42-2]

PTM databases

iPTMnetⁱ	Q8IV42
PhosphoSitePlusⁱ	Q8IV42

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000179988
CleanExⁱ	HS_PSTK
ExpressionAtlasⁱ	Q8IV42 baseline and differential
Genevisibleⁱ	Q8IV42 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA037780 HPA037781

HPAⁱ

HPA037780
HPA037781

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	9606.ENSP00000357882

STRINGⁱ

9606.ENSP00000357882

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8IV42
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the L-seryl-tRNA(Sec) kinase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG4622 Eukaryota ENOG4111HI0 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017554
HOGENOMⁱ	HOG000115749
HOVERGENⁱ	HBG064780
InParanoidⁱ	Q8IV42
KEGG Orthology (KO) More...KOⁱ	K10837
PhylomeDBⁱ	Q8IV42
TreeFamⁱ	TF321264

Family and domain databases

InterProⁱ	View protein in InterPro IPR013641 KTI12/PSTK IPR020028 L-seryl-tRNA_Sec_kinase_euk IPR027417 P-loop_NTPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08433 KTI12, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 4 hits
TIGRFAMsⁱ	TIGR03575 selen_PSTK_euk, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: Q8IV42-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTAENIRGT GSDGPRKRGL CVLCGLPAAG KSTFARALAH RLQQEQGWAI 
        60         70         80         90        100
GVVAYDDVMP DAFLAGARAR PAPSQWKLLR QELLKYLEYF LMAVINGCQM 
       110        120        130        140        150
SVPPNRTEAM WEDFITCLKD QDLIFSAAFE AQSCYLLTKT AVSRPLFLVL 
       160        170        180        190        200
DDNFYYQSMR YEVYQLARKY SLGFCQLFLD CPLETCLQRN GQRPQALPPE 
       210        220        230        240        250
TIHLMGRKLE KPNPEKNAWE HNSLTIPSPA CASEASLEVT DLLLTALENP 
       260        270        280        290        300
VKYAEDNMEQ KDTDRIICST NILHKTDQTL RRIVSQTMKE AKGNQEAFSE 
       310        320        330        340 
MTFKQRWVRA NHAAIWRIIL GNEHIKCRSA KVGWLQCCRI EKRPLSTG

Length:348

Mass (Da):39,527

Last modified:October 17, 2006 - v2

Checksum:ⁱA7DDFF0BDC93BE4E

GO

Isoform 2 (identifier: Q8IV42-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
262-348: DTDRIICSTN...RIEKRPLSTG → VNFQCKFNVNEKEQLFVGRSFEPIIKCE

« Hide

        10         20         30         40         50
MKTAENIRGT GSDGPRKRGL CVLCGLPAAG KSTFARALAH RLQQEQGWAI 
        60         70         80         90        100
GVVAYDDVMP DAFLAGARAR PAPSQWKLLR QELLKYLEYF LMAVINGCQM 
       110        120        130        140        150
SVPPNRTEAM WEDFITCLKD QDLIFSAAFE AQSCYLLTKT AVSRPLFLVL 
       160        170        180        190        200
DDNFYYQSMR YEVYQLARKY SLGFCQLFLD CPLETCLQRN GQRPQALPPE 
       210        220        230        240        250
TIHLMGRKLE KPNPEKNAWE HNSLTIPSPA CASEASLEVT DLLLTALENP 
       260        270        280 
VKYAEDNMEQ KVNFQCKFNV NEKEQLFVGR SFEPIIKCE

Show »

Length:289

Mass (Da):32,749

Checksum:ⁱ2DB837E8522CF6E6

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_028156	206	G → R1 PublicationCorresponds to variant dbSNP:rs3736582Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_014989	262 – 348	DTDRI…PLSTG → VNFQCKFNVNEKEQLFVGRS FEPIIKCE in isoform 2. 1 PublicationAdd BLAST		87

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK127173 mRNA Translation: BAC86867.1 BC035344 mRNA Translation: AAH35344.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS7633.1 [Q8IV42-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_699167.2, NM_153336.2 [Q8IV42-1]
UniGeneⁱ	Hs.281004

Genome annotation databases

Ensemblⁱ	ENST00000368887; ENSP00000357882; ENSG00000179988 [Q8IV42-1] ENST00000405485; ENSP00000384764; ENSG00000179988 [Q8IV42-1]
GeneIDⁱ	118672
KEGGⁱ	hsa:118672
UCSC genome browser More...UCSCⁱ	uc001lgy.2 human [Q8IV42-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Protein	Similar proteins		Species	Score	Length
Q8IV42	UPI0005F438B7		COLAP		387
UPI000D311F00		MACNE		354
UPI000C29AA55		Ugandan red Colobus		354
UPI0005F46D5B		CERAT		350
UPI00045DC97D		CHLSB		350
+6
Q8IV42-2	Phosphoseryl-tRNA kinase		MACMU		333
Phosphoseryl-tRNA kinase		COLAP		328
Phosphoseryl-tRNA kinase		RHIRO		328
Phosphoseryl-tRNA kinase		RHIBE		328
Phosphoseryl-tRNA kinase		PAPAN		323
+4

Protein	Similar proteins		Species	Score	Length
Q8IV42	UPI0005F438B7		COLAP		387
UPI000C29AA55		Ugandan red Colobus		354
UPI000D311F00		MACNE		354
UPI0005F46D5B		CERAT		350
UPI00045DC97D		CHLSB		350
+37

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK127173 mRNA Translation: BAC86867.1 BC035344 mRNA Translation: AAH35344.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS7633.1 [Q8IV42-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_699167.2, NM_153336.2 [Q8IV42-1]
UniGeneⁱ	Hs.281004

3D structure databases

ProteinModelPortalⁱ	Q8IV42
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	9606.ENSP00000357882

STRINGⁱ

9606.ENSP00000357882

PTM databases

iPTMnetⁱ	Q8IV42
PhosphoSitePlusⁱ	Q8IV42

Polymorphism and mutation databases

BioMutaⁱ	PSTK
DMDMⁱ	116242735

Proteomic databases

PaxDbⁱ	Q8IV42
PeptideAtlas More...PeptideAtlasⁱ	Q8IV42
PRIDEⁱ	Q8IV42
ProteomicsDBⁱ	70656 70657 [Q8IV42-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	118672
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000368887; ENSP00000357882; ENSG00000179988 [Q8IV42-1] ENST00000405485; ENSP00000384764; ENSG00000179988 [Q8IV42-1]
GeneIDⁱ	118672
KEGGⁱ	hsa:118672
UCSC genome browser More...UCSCⁱ	uc001lgy.2 human [Q8IV42-1]

Organism-specific databases

CTDⁱ	118672
EuPathDBⁱ	HostDB:ENSG00000179988.13
GeneCardsⁱ	PSTK
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:28578 PSTK
Human Protein Atlas More...HPAⁱ	HPA037780 HPA037781
MIMⁱ	611310 gene
neXtProtⁱ	NX_Q8IV42
Open Targets More...OpenTargetsⁱ	ENSG00000179988
PharmGKBⁱ	PA162400268
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG4622 Eukaryota ENOG4111HI0 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017554
HOGENOMⁱ	HOG000115749
HOVERGENⁱ	HBG064780
InParanoidⁱ	Q8IV42
KEGG Orthology (KO) More...KOⁱ	K10837
PhylomeDBⁱ	Q8IV42
TreeFamⁱ	TF321264

Enzyme and pathway databases

UniPathwayⁱ	UPA00906; UER00897
Reactomeⁱ	R-HSA-2408557 Selenocysteine synthesis

Miscellaneous databases

ChiTaRSⁱ	PSTK human
GenomeRNAiⁱ	118672
Protein Ontology More...PROⁱ	PR:Q8IV42
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000179988
CleanExⁱ	HS_PSTK
ExpressionAtlasⁱ	Q8IV42 baseline and differential
Genevisibleⁱ	Q8IV42 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR013641 KTI12/PSTK IPR020028 L-seryl-tRNA_Sec_kinase_euk IPR027417 P-loop_NTPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08433 KTI12, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 4 hits
TIGRFAMsⁱ	TIGR03575 selen_PSTK_euk, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PSTK_HUMAN
Accessionⁱ	Q8IV42Primary (citable) accession number: Q8IV42 Secondary accession number(s): Q6ZSS9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
	Last sequence update:	October 17, 2006
	Last modified:	June 20, 2018
	This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

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UniProtKB - Q8IV42 (PSTK_HUMAN)

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L-seryl-tRNA(Sec) kinase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: selenocysteinyl-tRNA(Sec) biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ