UniProtKB - Q8IV42 (PSTK_HUMAN)
Protein
L-seryl-tRNA(Sec) kinase
Gene
PSTK
Organism
Homo sapiens (Human)
Status
Functioni
Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis.By similarity
Catalytic activityi
- EC:2.7.1.164
Cofactori
Mg2+By similarity
: selenocysteinyl-tRNA(Sec) biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- L-seryl-tRNA(Sec) kinase (PSTK)
- O-phosphoseryl-tRNA(Sec) selenium transferase, O-phosphoseryl-tRNA(Sec) selenium transferase (SEPSECS), O-phosphoseryl-tRNA(Sec) selenium transferase (SEPSECS), O-phosphoseryl-tRNA(Sec) selenium transferase (SEPSECS)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 25 – 32 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase activity Source: GO_Central
- L-seryl-tRNA(Sec) kinase activity Source: UniProtKB-EC
- tRNA binding Source: GO_Central
GO - Biological processi
- selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
- translation Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8IV42 |
Reactomei | R-HSA-2408557, Selenocysteine synthesis |
UniPathwayi | UPA00906;UER00897 |
Names & Taxonomyi
Protein namesi | Recommended name: L-seryl-tRNA(Sec) kinase (EC:2.7.1.164)Alternative name(s): O-phosphoseryl-tRNA(Sec) kinase |
Gene namesi | Name:PSTK Synonyms:C10orf89 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000179988.13 |
HGNCi | HGNC:28578, PSTK |
MIMi | 611310, gene |
neXtProti | NX_Q8IV42 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 118672 |
OpenTargetsi | ENSG00000179988 |
PharmGKBi | PA162400268 |
Miscellaneous databases
Pharosi | Q8IV42, Tdark |
Polymorphism and mutation databases
BioMutai | PSTK |
DMDMi | 116242735 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097080 | 1 – 348 | L-seryl-tRNA(Sec) kinaseAdd BLAST | 348 |
Proteomic databases
MassIVEi | Q8IV42 |
PaxDbi | Q8IV42 |
PeptideAtlasi | Q8IV42 |
PRIDEi | Q8IV42 |
ProteomicsDBi | 70656 [Q8IV42-1] 70657 [Q8IV42-2] |
PTM databases
iPTMneti | Q8IV42 |
PhosphoSitePlusi | Q8IV42 |
Expressioni
Gene expression databases
Bgeei | ENSG00000179988, Expressed in muscle tissue and 167 other tissues |
ExpressionAtlasi | Q8IV42, baseline and differential |
Genevisiblei | Q8IV42, HS |
Organism-specific databases
HPAi | ENSG00000179988, Low tissue specificity |
Interactioni
Protein-protein interaction databases
STRINGi | 9606.ENSP00000357882 |
Miscellaneous databases
RNActi | Q8IV42, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the L-seryl-tRNA(Sec) kinase family.Curated
Phylogenomic databases
eggNOGi | KOG4622, Eukaryota |
GeneTreei | ENSGT00390000017554 |
InParanoidi | Q8IV42 |
PhylomeDBi | Q8IV42 |
TreeFami | TF321264 |
Family and domain databases
InterProi | View protein in InterPro IPR013641, KTI12/PSTK IPR020028, L-seryl-tRNA_Sec_kinase_euk IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF08433, KTI12, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR03575, selen_PSTK_euk, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8IV42-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKTAENIRGT GSDGPRKRGL CVLCGLPAAG KSTFARALAH RLQQEQGWAI
60 70 80 90 100
GVVAYDDVMP DAFLAGARAR PAPSQWKLLR QELLKYLEYF LMAVINGCQM
110 120 130 140 150
SVPPNRTEAM WEDFITCLKD QDLIFSAAFE AQSCYLLTKT AVSRPLFLVL
160 170 180 190 200
DDNFYYQSMR YEVYQLARKY SLGFCQLFLD CPLETCLQRN GQRPQALPPE
210 220 230 240 250
TIHLMGRKLE KPNPEKNAWE HNSLTIPSPA CASEASLEVT DLLLTALENP
260 270 280 290 300
VKYAEDNMEQ KDTDRIICST NILHKTDQTL RRIVSQTMKE AKGNQEAFSE
310 320 330 340
MTFKQRWVRA NHAAIWRIIL GNEHIKCRSA KVGWLQCCRI EKRPLSTG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH7BYY4 | H7BYY4_HUMAN | Chromosome 10 open reading frame 89... | PSTK C10orf89, hCG_41432 | 358 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_028156 | 206 | G → R1 PublicationCorresponds to variant dbSNP:rs3736582Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014989 | 262 – 348 | DTDRI…PLSTG → VNFQCKFNVNEKEQLFVGRS FEPIIKCE in isoform 2. 1 PublicationAdd BLAST | 87 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK127173 mRNA Translation: BAC86867.1 BC035344 mRNA Translation: AAH35344.1 |
CCDSi | CCDS7633.1 [Q8IV42-1] |
RefSeqi | NP_699167.2, NM_153336.2 [Q8IV42-1] |
Genome annotation databases
Ensembli | ENST00000368887; ENSP00000357882; ENSG00000179988 [Q8IV42-1] ENST00000405485; ENSP00000384764; ENSG00000179988 [Q8IV42-1] |
GeneIDi | 118672 |
KEGGi | hsa:118672 |
UCSCi | uc001lgy.2, human [Q8IV42-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK127173 mRNA Translation: BAC86867.1 BC035344 mRNA Translation: AAH35344.1 |
CCDSi | CCDS7633.1 [Q8IV42-1] |
RefSeqi | NP_699167.2, NM_153336.2 [Q8IV42-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9606.ENSP00000357882 |
PTM databases
iPTMneti | Q8IV42 |
PhosphoSitePlusi | Q8IV42 |
Polymorphism and mutation databases
BioMutai | PSTK |
DMDMi | 116242735 |
Proteomic databases
MassIVEi | Q8IV42 |
PaxDbi | Q8IV42 |
PeptideAtlasi | Q8IV42 |
PRIDEi | Q8IV42 |
ProteomicsDBi | 70656 [Q8IV42-1] 70657 [Q8IV42-2] |
Protocols and materials databases
Antibodypediai | 46364, 60 antibodies |
DNASUi | 118672 |
Genome annotation databases
Ensembli | ENST00000368887; ENSP00000357882; ENSG00000179988 [Q8IV42-1] ENST00000405485; ENSP00000384764; ENSG00000179988 [Q8IV42-1] |
GeneIDi | 118672 |
KEGGi | hsa:118672 |
UCSCi | uc001lgy.2, human [Q8IV42-1] |
Organism-specific databases
CTDi | 118672 |
DisGeNETi | 118672 |
EuPathDBi | HostDB:ENSG00000179988.13 |
GeneCardsi | PSTK |
HGNCi | HGNC:28578, PSTK |
HPAi | ENSG00000179988, Low tissue specificity |
MIMi | 611310, gene |
neXtProti | NX_Q8IV42 |
OpenTargetsi | ENSG00000179988 |
PharmGKBi | PA162400268 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4622, Eukaryota |
GeneTreei | ENSGT00390000017554 |
InParanoidi | Q8IV42 |
PhylomeDBi | Q8IV42 |
TreeFami | TF321264 |
Enzyme and pathway databases
UniPathwayi | UPA00906;UER00897 |
PathwayCommonsi | Q8IV42 |
Reactomei | R-HSA-2408557, Selenocysteine synthesis |
Miscellaneous databases
BioGRID-ORCSi | 118672, 346 hits in 840 CRISPR screens |
ChiTaRSi | PSTK, human |
GenomeRNAii | 118672 |
Pharosi | Q8IV42, Tdark |
PROi | PR:Q8IV42 |
RNActi | Q8IV42, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000179988, Expressed in muscle tissue and 167 other tissues |
ExpressionAtlasi | Q8IV42, baseline and differential |
Genevisiblei | Q8IV42, HS |
Family and domain databases
InterProi | View protein in InterPro IPR013641, KTI12/PSTK IPR020028, L-seryl-tRNA_Sec_kinase_euk IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF08433, KTI12, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR03575, selen_PSTK_euk, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSTK_HUMAN | |
Accessioni | Q8IV42Primary (citable) accession number: Q8IV42 Secondary accession number(s): Q6ZSS9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | October 17, 2006 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations