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Protein

Ribosomal oxygenase 2

Gene

RIOX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.8 Publications

Catalytic activityi

L-histidine-[60S ribosomal protein L27a] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L27a] + succinate + CO2.1 Publication

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi179Iron; catalyticCurated1
Metal bindingi181Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi240Iron; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processRibosome biogenesis, Transcription, Transcription regulation
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-3214842 HDMs demethylate histones

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal oxygenase 2Imported
Alternative name(s):
60S ribosomal protein L27a histidine hydroxylase
Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA (EC:1.14.11.-)
Histone lysine demethylase MINA
MYC-induced nuclear antigen
Mineral dust-induced gene protein
Nucleolar protein 52
Ribosomal oxygenase MINA
Short name:
ROX
Gene namesi
Name:RIOX2Imported
Synonyms:MDIGImported, MINA, MINA53Imported, NO52Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000170854.17
HGNCiHGNC:19441 RIOX2
MIMi612049 gene
neXtProtiNX_Q8IUF8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi179H → Y: Abolishes demethylase activity. 1 Publication1

Organism-specific databases

DisGeNETi84864
OpenTargetsiENSG00000170854
PharmGKBiPA134991047

Polymorphism and mutation databases

BioMutaiMINA
DMDMi74750624

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003083771 – 465Ribosomal oxygenase 2Add BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei309PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IUF8
MaxQBiQ8IUF8
PaxDbiQ8IUF8
PeptideAtlasiQ8IUF8
PRIDEiQ8IUF8
ProteomicsDBi70558
70559 [Q8IUF8-2]
70560 [Q8IUF8-3]
70561 [Q8IUF8-4]
TopDownProteomicsiQ8IUF8-2 [Q8IUF8-2]

PTM databases

iPTMnetiQ8IUF8
PhosphoSitePlusiQ8IUF8

Expressioni

Tissue specificityi

Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.4 Publications

Inductioni

Up-regulated in response to MYC, in alveolar macrophages from coal miners and in silica particle-treated A549 lung cancer cells.2 Publications

Gene expression databases

BgeeiENSG00000170854 Expressed in 231 organ(s), highest expression level in amniotic fluid
CleanExiHS_MINA
ExpressionAtlasiQ8IUF8 baseline and differential
GenevisibleiQ8IUF8 HS

Organism-specific databases

HPAiCAB013458
HPA007603
HPA008080

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-751580,EBI-751580

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124309, 35 interactors
DIPiDIP-28141N
IntActiQ8IUF8, 14 interactors
MINTiQ8IUF8
STRINGi9606.ENSP00000328251

Chemistry databases

BindingDBiQ8IUF8

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8IUF8
SMRiQ8IUF8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8IUF8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 271JmjCPROSITE-ProRule annotationAdd BLAST133

Sequence similaritiesi

Belongs to the ROX family. MINA53 subfamily.Curated

Phylogenomic databases

eggNOGiKOG3706 Eukaryota
ENOG410YNEJ LUCA
GeneTreeiENSGT00390000000083
HOVERGENiHBG055265
InParanoidiQ8IUF8
KOiK21760
OMAiFDTAKED
OrthoDBiEOG091G04K2
PhylomeDBiQ8IUF8
TreeFamiTF318659

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
IPR039994 JmjC_protein
PANTHERiPTHR13096 PTHR13096, 1 hit
PfamiView protein in Pfam
PF08007 Cupin_4, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 16 Publications (identifier: Q8IUF8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK
60 70 80 90 100
TETFFKEFWE QKPLLIQRDD PALATYYGSL FKLTDLKSLC SRGMYYGRDV
110 120 130 140 150
NVCRCVNGKK KVLNKDGKAH FLQLRKDFDQ KRATIQFHQP QRFKDELWRI
160 170 180 190 200
QEKLECYFGS LVGSNVYITP AGSQGLPPHY DDVEVFILQL EGEKHWRLYH
210 220 230 240 250
PTVPLAREYS VEAEERIGRP VHEFMLKPGD LLYFPRGTIH QADTPAGLAH
260 270 280 290 300
STHVTISTYQ NNSWGDFLLD TISGLVFDTA KEDVELRTGI PRQLLLQVES
310 320 330 340 350
TTVATRRLSG FLRTLADRLE GTKELLSSDM KKDFIMHRLP PYSAGDGAEL
360 370 380 390 400
STPGGKLPRL DSVVRLQFKD HIVLTVLPDQ DQSDEAQEKM VYIYHSLKNS
410 420 430 440 450
RETHMMGNEE ETEFHGLRFP LSHLDALKQI WNSPAISVKD LKLTTDEEKE
460
SLVLSLWTEC LIQVV
Length:465
Mass (Da):52,800
Last modified:March 1, 2003 - v1
Checksum:iB59F8AE9C802FEB0
GO
Isoform 21 Publication (identifier: Q8IUF8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-280: SWGDFLLDTISGLVFDTA → DAGARMRRCDLRAIAPQK
     281-465: Missing.

Show »
Length:280
Mass (Da):31,818
Checksum:i8B69C261ABC594EB
GO
Isoform 31 Publication (identifier: Q8IUF8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-254: Missing.
     255-261: TISTYQN → MLLQVPC

Show »
Length:211
Mass (Da):23,909
Checksum:iDA5AB0F080853151
GO
Isoform 4 (identifier: Q8IUF8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     297-297: Missing.

Show »
Length:464
Mass (Da):52,672
Checksum:i24902143244D1A79
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y9L8H0Y9L8_HUMAN
Ribosomal oxygenase 2
RIOX2
108Annotation score:
D6RCB6D6RCB6_HUMAN
Ribosomal oxygenase 2
RIOX2
112Annotation score:
H0Y8K4H0Y8K4_HUMAN
Ribosomal oxygenase 2
RIOX2
27Annotation score:

Sequence cautioni

The sequence CAH10679 differs from that shown. Reason: Erroneous termination at position 431. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87K → E in BAC16359 (PubMed:12091391).Curated1
Sequence conflicti107N → S in BAC16359 (PubMed:12091391).Curated1
Sequence conflicti159G → S in BAB55024 (PubMed:14702039).Curated1
Sequence conflicti221V → A in BAC16359 (PubMed:12091391).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05407917A → P. Corresponds to variant dbSNP:rs35391656Ensembl.1
Natural variantiVAR_062241201P → L. Corresponds to variant dbSNP:rs56183666Ensembl.1
Natural variantiVAR_036811386A → T4 PublicationsCorresponds to variant dbSNP:rs2172257Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0525871 – 254Missing in isoform 3. 1 PublicationAdd BLAST254
Alternative sequenceiVSP_052588255 – 261TISTYQN → MLLQVPC in isoform 3. 1 Publication7
Alternative sequenceiVSP_052589263 – 280SWGDF…VFDTA → DAGARMRRCDLRAIAPQK in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_052590281 – 465Missing in isoform 2. 1 PublicationAdd BLAST185
Alternative sequenceiVSP_038373297Missing in isoform 4. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083189 mRNA Translation: BAC16537.1
AB083190 mRNA Translation: BAC16358.1
AB083191 mRNA Translation: BAC16359.1
AB083192 mRNA Translation: BAC16360.1
AB083193 mRNA Translation: BAC16361.1
AY390536 mRNA Translation: AAR27293.1
AY302110 mRNA Translation: AAP59421.1
AY456380 mRNA Translation: AAR21572.1
DQ453796 mRNA Translation: ABE28016.1
AK027299 mRNA Translation: BAB55024.1
AC026100 Genomic DNA No translation available.
AC110491 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79872.1
CH471052 Genomic DNA Translation: EAW79873.1
BC014928 mRNA Translation: AAH14928.1
CR627479 mRNA Translation: CAH10679.1 Different termination.
CCDSiCCDS2929.1 [Q8IUF8-4]
CCDS43114.1 [Q8IUF8-1]
RefSeqiNP_001035998.1, NM_001042533.2 [Q8IUF8-1]
NP_001248758.1, NM_001261829.1 [Q8IUF8-4]
NP_116167.3, NM_032778.5 [Q8IUF8-4]
NP_694822.2, NM_153182.3 [Q8IUF8-1]
XP_005247895.1, XM_005247838.4 [Q8IUF8-1]
UniGeneiHs.570562
Hs.742206

Genome annotation databases

EnsembliENST00000333396; ENSP00000328251; ENSG00000170854 [Q8IUF8-1]
ENST00000360258; ENSP00000353395; ENSG00000170854 [Q8IUF8-4]
ENST00000394198; ENSP00000377748; ENSG00000170854 [Q8IUF8-1]
ENST00000514314; ENSP00000424955; ENSG00000170854 [Q8IUF8-2]
GeneIDi84864
KEGGihsa:84864
UCSCiuc003drz.3 human [Q8IUF8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083189 mRNA Translation: BAC16537.1
AB083190 mRNA Translation: BAC16358.1
AB083191 mRNA Translation: BAC16359.1
AB083192 mRNA Translation: BAC16360.1
AB083193 mRNA Translation: BAC16361.1
AY390536 mRNA Translation: AAR27293.1
AY302110 mRNA Translation: AAP59421.1
AY456380 mRNA Translation: AAR21572.1
DQ453796 mRNA Translation: ABE28016.1
AK027299 mRNA Translation: BAB55024.1
AC026100 Genomic DNA No translation available.
AC110491 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79872.1
CH471052 Genomic DNA Translation: EAW79873.1
BC014928 mRNA Translation: AAH14928.1
CR627479 mRNA Translation: CAH10679.1 Different termination.
CCDSiCCDS2929.1 [Q8IUF8-4]
CCDS43114.1 [Q8IUF8-1]
RefSeqiNP_001035998.1, NM_001042533.2 [Q8IUF8-1]
NP_001248758.1, NM_001261829.1 [Q8IUF8-4]
NP_116167.3, NM_032778.5 [Q8IUF8-4]
NP_694822.2, NM_153182.3 [Q8IUF8-1]
XP_005247895.1, XM_005247838.4 [Q8IUF8-1]
UniGeneiHs.570562
Hs.742206

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XDVX-ray2.57A26-465[»]
4BU2X-ray2.78A26-465[»]
4BXFX-ray2.05A/B26-465[»]
ProteinModelPortaliQ8IUF8
SMRiQ8IUF8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124309, 35 interactors
DIPiDIP-28141N
IntActiQ8IUF8, 14 interactors
MINTiQ8IUF8
STRINGi9606.ENSP00000328251

Chemistry databases

BindingDBiQ8IUF8

PTM databases

iPTMnetiQ8IUF8
PhosphoSitePlusiQ8IUF8

Polymorphism and mutation databases

BioMutaiMINA
DMDMi74750624

Proteomic databases

EPDiQ8IUF8
MaxQBiQ8IUF8
PaxDbiQ8IUF8
PeptideAtlasiQ8IUF8
PRIDEiQ8IUF8
ProteomicsDBi70558
70559 [Q8IUF8-2]
70560 [Q8IUF8-3]
70561 [Q8IUF8-4]
TopDownProteomicsiQ8IUF8-2 [Q8IUF8-2]

Protocols and materials databases

DNASUi84864
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000333396; ENSP00000328251; ENSG00000170854 [Q8IUF8-1]
ENST00000360258; ENSP00000353395; ENSG00000170854 [Q8IUF8-4]
ENST00000394198; ENSP00000377748; ENSG00000170854 [Q8IUF8-1]
ENST00000514314; ENSP00000424955; ENSG00000170854 [Q8IUF8-2]
GeneIDi84864
KEGGihsa:84864
UCSCiuc003drz.3 human [Q8IUF8-1]

Organism-specific databases

CTDi84864
DisGeNETi84864
EuPathDBiHostDB:ENSG00000170854.17
GeneCardsiRIOX2
HGNCiHGNC:19441 RIOX2
HPAiCAB013458
HPA007603
HPA008080
MIMi612049 gene
neXtProtiNX_Q8IUF8
OpenTargetsiENSG00000170854
PharmGKBiPA134991047
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3706 Eukaryota
ENOG410YNEJ LUCA
GeneTreeiENSGT00390000000083
HOVERGENiHBG055265
InParanoidiQ8IUF8
KOiK21760
OMAiFDTAKED
OrthoDBiEOG091G04K2
PhylomeDBiQ8IUF8
TreeFamiTF318659

Enzyme and pathway databases

ReactomeiR-HSA-3214842 HDMs demethylate histones

Miscellaneous databases

EvolutionaryTraceiQ8IUF8
GeneWikiiMINA
MYC-induced_nuclear_antigen
GenomeRNAii84864
PROiPR:Q8IUF8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170854 Expressed in 231 organ(s), highest expression level in amniotic fluid
CleanExiHS_MINA
ExpressionAtlasiQ8IUF8 baseline and differential
GenevisibleiQ8IUF8 HS

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
IPR039994 JmjC_protein
PANTHERiPTHR13096 PTHR13096, 1 hit
PfamiView protein in Pfam
PF08007 Cupin_4, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIOX2_HUMAN
AccessioniPrimary (citable) accession number: Q8IUF8
Secondary accession number(s): D3DN35
, Q6AHW4, Q6SKS0, Q8IU69, Q8IUF6, Q8IUF7, Q96C17, Q96KB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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