UniProtKB - Q8IUF8 (RIOX2_HUMAN)
Protein
Ribosomal oxygenase 2
Gene
RIOX2
Organism
Homo sapiens (Human)
Status
Functioni
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.8 Publications
Catalytic activityi
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 179 | Iron; catalyticCurated | 1 | |
Metal bindingi | 181 | Iron; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 240 | Iron; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 2-oxoglutarate-dependent dioxygenase activity Source: GO_Central
- histone demethylase activity (H3-K36 specific) Source: GO_Central
- histone demethylase activity (H3-K4 specific) Source: GO_Central
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- transcription corepressor activity Source: Ensembl
GO - Biological processi
- histone H3-K36 demethylation Source: GO_Central
- histone H3-K4 demethylation Source: GO_Central
- ribosome biogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Ribosome biogenesis, Transcription, Transcription regulation |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8IUF8 |
Reactomei | R-HSA-3214842, HDMs demethylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal oxygenase 2ImportedAlternative name(s): 60S ribosomal protein L27a histidine hydroxylase Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA (EC:1.14.11.-) Histone lysine demethylase MINA MYC-induced nuclear antigen Mineral dust-induced gene protein Nucleolar protein 52 Ribosomal oxygenase MINA Short name: ROX |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000170854.17 |
HGNCi | HGNC:19441, RIOX2 |
MIMi | 612049, gene |
neXtProti | NX_Q8IUF8 |
Subcellular locationi
Cytosol
- cytosol Source: Ensembl
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
Other locations
- transcription regulator complex Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 179 | H → Y: Abolishes demethylase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 84864 |
OpenTargetsi | ENSG00000170854 |
PharmGKBi | PA134991047 |
Miscellaneous databases
Pharosi | Q8IUF8, Tbio |
Polymorphism and mutation databases
BioMutai | RIOX2 |
DMDMi | 74750624 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000308377 | 1 – 465 | Ribosomal oxygenase 2Add BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 309 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8IUF8 |
jPOSTi | Q8IUF8 |
MassIVEi | Q8IUF8 |
MaxQBi | Q8IUF8 |
PaxDbi | Q8IUF8 |
PeptideAtlasi | Q8IUF8 |
PRIDEi | Q8IUF8 |
ProteomicsDBi | 70558 [Q8IUF8-1] 70559 [Q8IUF8-2] 70560 [Q8IUF8-3] 70561 [Q8IUF8-4] |
TopDownProteomicsi | Q8IUF8-2 [Q8IUF8-2] |
PTM databases
iPTMneti | Q8IUF8 |
PhosphoSitePlusi | Q8IUF8 |
Expressioni
Tissue specificityi
Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.4 Publications
Inductioni
Up-regulated in response to MYC, in alveolar macrophages from coal miners and in silica particle-treated A549 lung cancer cells.2 Publications
Gene expression databases
Bgeei | ENSG00000170854, Expressed in thyroid gland and 244 other tissues |
ExpressionAtlasi | Q8IUF8, baseline and differential |
Genevisiblei | Q8IUF8, HS |
Organism-specific databases
HPAi | ENSG00000170854, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsQ8IUF8
With | #Exp. | IntAct |
---|---|---|
LNX1 [Q8TBB1] | 3 | EBI-751580,EBI-739832 |
itself | 5 | EBI-751580,EBI-751580 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 124309, 42 interactors |
DIPi | DIP-28141N |
IntActi | Q8IUF8, 27 interactors |
MINTi | Q8IUF8 |
STRINGi | 9606.ENSP00000328251 |
Chemistry databases
BindingDBi | Q8IUF8 |
Miscellaneous databases
RNActi | Q8IUF8, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8IUF8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8IUF8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 139 – 271 | JmjCPROSITE-ProRule annotationAdd BLAST | 133 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3706, Eukaryota |
GeneTreei | ENSGT00390000000083 |
HOGENOMi | CLU_013645_0_1_1 |
InParanoidi | Q8IUF8 |
OMAi | RPTHEFT |
OrthoDBi | 693909at2759 |
PhylomeDBi | Q8IUF8 |
TreeFami | TF318659 |
Family and domain databases
InterProi | View protein in InterPro IPR003347, JmjC_dom IPR039994, JmjC_protein |
PANTHERi | PTHR13096, PTHR13096, 1 hit |
Pfami | View protein in Pfam PF08007, Cupin_4, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 16 Publications (identifier: Q8IUF8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK
60 70 80 90 100
TETFFKEFWE QKPLLIQRDD PALATYYGSL FKLTDLKSLC SRGMYYGRDV
110 120 130 140 150
NVCRCVNGKK KVLNKDGKAH FLQLRKDFDQ KRATIQFHQP QRFKDELWRI
160 170 180 190 200
QEKLECYFGS LVGSNVYITP AGSQGLPPHY DDVEVFILQL EGEKHWRLYH
210 220 230 240 250
PTVPLAREYS VEAEERIGRP VHEFMLKPGD LLYFPRGTIH QADTPAGLAH
260 270 280 290 300
STHVTISTYQ NNSWGDFLLD TISGLVFDTA KEDVELRTGI PRQLLLQVES
310 320 330 340 350
TTVATRRLSG FLRTLADRLE GTKELLSSDM KKDFIMHRLP PYSAGDGAEL
360 370 380 390 400
STPGGKLPRL DSVVRLQFKD HIVLTVLPDQ DQSDEAQEKM VYIYHSLKNS
410 420 430 440 450
RETHMMGNEE ETEFHGLRFP LSHLDALKQI WNSPAISVKD LKLTTDEEKE
460
SLVLSLWTEC LIQVV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y9L8 | H0Y9L8_HUMAN | Bifunctional lysine-specific demeth... | RIOX2 | 108 | Annotation score: | ||
D6RCB6 | D6RCB6_HUMAN | Bifunctional lysine-specific demeth... | RIOX2 | 112 | Annotation score: | ||
H0Y8K4 | H0Y8K4_HUMAN | Ribosomal oxygenase 2 | RIOX2 | 27 | Annotation score: |
Sequence cautioni
The sequence CAH10679 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 87 | K → E in BAC16359 (PubMed:12091391).Curated | 1 | |
Sequence conflicti | 107 | N → S in BAC16359 (PubMed:12091391).Curated | 1 | |
Sequence conflicti | 159 | G → S in BAB55024 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 221 | V → A in BAC16359 (PubMed:12091391).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_054079 | 17 | A → P. Corresponds to variant dbSNP:rs35391656Ensembl. | 1 | |
Natural variantiVAR_062241 | 201 | P → L. Corresponds to variant dbSNP:rs56183666Ensembl. | 1 | |
Natural variantiVAR_036811 | 386 | A → T4 PublicationsCorresponds to variant dbSNP:rs2172257Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052587 | 1 – 254 | Missing in isoform 3. 1 PublicationAdd BLAST | 254 | |
Alternative sequenceiVSP_052588 | 255 – 261 | TISTYQN → MLLQVPC in isoform 3. 1 Publication | 7 | |
Alternative sequenceiVSP_052589 | 263 – 280 | SWGDF…VFDTA → DAGARMRRCDLRAIAPQK in isoform 2. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_052590 | 281 – 465 | Missing in isoform 2. 1 PublicationAdd BLAST | 185 | |
Alternative sequenceiVSP_038373 | 297 | Missing in isoform 4. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB083189 mRNA Translation: BAC16537.1 AB083190 mRNA Translation: BAC16358.1 AB083191 mRNA Translation: BAC16359.1 AB083192 mRNA Translation: BAC16360.1 AB083193 mRNA Translation: BAC16361.1 AY390536 mRNA Translation: AAR27293.1 AY302110 mRNA Translation: AAP59421.1 AY456380 mRNA Translation: AAR21572.1 DQ453796 mRNA Translation: ABE28016.1 AK027299 mRNA Translation: BAB55024.1 AC026100 Genomic DNA No translation available. AC110491 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79872.1 CH471052 Genomic DNA Translation: EAW79873.1 BC014928 mRNA Translation: AAH14928.1 CR627479 mRNA Translation: CAH10679.1 Different termination. |
CCDSi | CCDS2929.1 [Q8IUF8-4] CCDS43114.1 [Q8IUF8-1] |
RefSeqi | NP_001035998.1, NM_001042533.2 [Q8IUF8-1] NP_001248758.1, NM_001261829.1 [Q8IUF8-4] NP_116167.3, NM_032778.5 [Q8IUF8-4] NP_694822.2, NM_153182.3 [Q8IUF8-1] XP_005247895.1, XM_005247838.4 [Q8IUF8-1] |
Genome annotation databases
Ensembli | ENST00000333396; ENSP00000328251; ENSG00000170854 [Q8IUF8-1] ENST00000360258; ENSP00000353395; ENSG00000170854 [Q8IUF8-4] ENST00000394198; ENSP00000377748; ENSG00000170854 [Q8IUF8-1] ENST00000514314; ENSP00000424955; ENSG00000170854 [Q8IUF8-2] |
GeneIDi | 84864 |
KEGGi | hsa:84864 |
UCSCi | uc003drz.3, human [Q8IUF8-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB083189 mRNA Translation: BAC16537.1 AB083190 mRNA Translation: BAC16358.1 AB083191 mRNA Translation: BAC16359.1 AB083192 mRNA Translation: BAC16360.1 AB083193 mRNA Translation: BAC16361.1 AY390536 mRNA Translation: AAR27293.1 AY302110 mRNA Translation: AAP59421.1 AY456380 mRNA Translation: AAR21572.1 DQ453796 mRNA Translation: ABE28016.1 AK027299 mRNA Translation: BAB55024.1 AC026100 Genomic DNA No translation available. AC110491 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79872.1 CH471052 Genomic DNA Translation: EAW79873.1 BC014928 mRNA Translation: AAH14928.1 CR627479 mRNA Translation: CAH10679.1 Different termination. |
CCDSi | CCDS2929.1 [Q8IUF8-4] CCDS43114.1 [Q8IUF8-1] |
RefSeqi | NP_001035998.1, NM_001042533.2 [Q8IUF8-1] NP_001248758.1, NM_001261829.1 [Q8IUF8-4] NP_116167.3, NM_032778.5 [Q8IUF8-4] NP_694822.2, NM_153182.3 [Q8IUF8-1] XP_005247895.1, XM_005247838.4 [Q8IUF8-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XDV | X-ray | 2.57 | A | 26-465 | [»] | |
4BU2 | X-ray | 2.78 | A | 26-465 | [»] | |
4BXF | X-ray | 2.05 | A/B | 26-465 | [»] | |
SMRi | Q8IUF8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124309, 42 interactors |
DIPi | DIP-28141N |
IntActi | Q8IUF8, 27 interactors |
MINTi | Q8IUF8 |
STRINGi | 9606.ENSP00000328251 |
Chemistry databases
BindingDBi | Q8IUF8 |
PTM databases
iPTMneti | Q8IUF8 |
PhosphoSitePlusi | Q8IUF8 |
Polymorphism and mutation databases
BioMutai | RIOX2 |
DMDMi | 74750624 |
Proteomic databases
EPDi | Q8IUF8 |
jPOSTi | Q8IUF8 |
MassIVEi | Q8IUF8 |
MaxQBi | Q8IUF8 |
PaxDbi | Q8IUF8 |
PeptideAtlasi | Q8IUF8 |
PRIDEi | Q8IUF8 |
ProteomicsDBi | 70558 [Q8IUF8-1] 70559 [Q8IUF8-2] 70560 [Q8IUF8-3] 70561 [Q8IUF8-4] |
TopDownProteomicsi | Q8IUF8-2 [Q8IUF8-2] |
Protocols and materials databases
Antibodypediai | 2005, 370 antibodies |
DNASUi | 84864 |
Genome annotation databases
Ensembli | ENST00000333396; ENSP00000328251; ENSG00000170854 [Q8IUF8-1] ENST00000360258; ENSP00000353395; ENSG00000170854 [Q8IUF8-4] ENST00000394198; ENSP00000377748; ENSG00000170854 [Q8IUF8-1] ENST00000514314; ENSP00000424955; ENSG00000170854 [Q8IUF8-2] |
GeneIDi | 84864 |
KEGGi | hsa:84864 |
UCSCi | uc003drz.3, human [Q8IUF8-1] |
Organism-specific databases
CTDi | 84864 |
DisGeNETi | 84864 |
EuPathDBi | HostDB:ENSG00000170854.17 |
GeneCardsi | RIOX2 |
HGNCi | HGNC:19441, RIOX2 |
HPAi | ENSG00000170854, Low tissue specificity |
MIMi | 612049, gene |
neXtProti | NX_Q8IUF8 |
OpenTargetsi | ENSG00000170854 |
PharmGKBi | PA134991047 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3706, Eukaryota |
GeneTreei | ENSGT00390000000083 |
HOGENOMi | CLU_013645_0_1_1 |
InParanoidi | Q8IUF8 |
OMAi | RPTHEFT |
OrthoDBi | 693909at2759 |
PhylomeDBi | Q8IUF8 |
TreeFami | TF318659 |
Enzyme and pathway databases
PathwayCommonsi | Q8IUF8 |
Reactomei | R-HSA-3214842, HDMs demethylate histones |
Miscellaneous databases
BioGRID-ORCSi | 84864, 6 hits in 846 CRISPR screens |
ChiTaRSi | MINA, human |
EvolutionaryTracei | Q8IUF8 |
GeneWikii | MINA MYC-induced_nuclear_antigen |
GenomeRNAii | 84864 |
Pharosi | Q8IUF8, Tbio |
PROi | PR:Q8IUF8 |
RNActi | Q8IUF8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170854, Expressed in thyroid gland and 244 other tissues |
ExpressionAtlasi | Q8IUF8, baseline and differential |
Genevisiblei | Q8IUF8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR003347, JmjC_dom IPR039994, JmjC_protein |
PANTHERi | PTHR13096, PTHR13096, 1 hit |
Pfami | View protein in Pfam PF08007, Cupin_4, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIOX2_HUMAN | |
Accessioni | Q8IUF8Primary (citable) accession number: Q8IUF8 Secondary accession number(s): D3DN35 Q96KB0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations