UniProtKB - Q8IUD6 (RN135_HUMAN)
E3 ubiquitin-protein ligase RNF135
RNF135
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 21 – 63 | RING-typePROSITE-ProRule annotationAdd BLAST | 43 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- ribonucleoprotein complex binding Source: UniProtKB
- RIG-I binding Source: UniProtKB
- ubiquitin protein ligase activity Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- antiviral innate immune response Source: UniProtKB
- free ubiquitin chain polymerization Source: UniProtKB
- innate immune response Source: Reactome
- negative regulation of type I interferon production Source: Reactome
- positive regulation of interferon-beta production Source: UniProtKB
- protein homooligomerization Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of innate immune response Source: UniProtKB
- RIG-I signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Immunity, Innate immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8IUD6 |
Reactomei | R-HSA-168928, DDX58/IFIH1-mediated induction of interferon-alpha/beta R-HSA-5689896, Ovarian tumor domain proteases R-HSA-918233, TRAF3-dependent IRF activation pathway R-HSA-933541, TRAF6 mediated IRF7 activation R-HSA-933542, TRAF6 mediated NF-kB activation R-HSA-933543, NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF135Curated (EC:2.3.2.271 Publication)Alternative name(s): RIG-I E3 ubiquitin ligase1 Publication Short name: REUL1 Publication RING finger protein 135 RING finger protein leading to RIG-I activation1 Publication Short name: Riplet1 Publication RING-type E3 ubiquitin transferase RNF135Curated |
Gene namesi | Name:RNF135Imported ORF Names:L13 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000181481.13 |
HGNCi | HGNC:21158, RNF135 |
MIMi | 611358, gene |
neXtProti | NX_Q8IUD6 |
Subcellular locationi
Other locations
- Cytoplasm 2 Publications
- Stress granule 1 Publication
Cytosol
- cytosol Source: Reactome
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: UniProtKB
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 16 – 18 | EDD → AAA: Prevents degradation by hepatitis C virus NS3/NS4A. 1 Publication | 3 | |
Mutagenesisi | 21 | C → A: Loss of function in RIG-I signaling pathway; when associated with A-24. 1 Publication | 1 | |
Mutagenesisi | 24 | C → A: Loss of function in RIG-I signaling pathway; when associated with A-21. 1 Publication | 1 |
Keywords - Diseasei
Disease mutationOrganism-specific databases
DisGeNETi | 84282 |
MalaCardsi | RNF135 |
OpenTargetsi | ENSG00000181481 |
Orphaneti | 137634, Overgrowth-macrocephaly-facial dysmorphism syndrome |
PharmGKBi | PA134978537 |
Miscellaneous databases
Pharosi | Q8IUD6, Tbio |
Polymorphism and mutation databases
BioMutai | RNF135 |
DMDMi | 269849639 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000280557 | 1 – 432 | E3 ubiquitin-protein ligase RNF135Add BLAST | 432 |
Post-translational modificationi
Proteomic databases
EPDi | Q8IUD6 |
jPOSTi | Q8IUD6 |
MassIVEi | Q8IUD6 |
MaxQBi | Q8IUD6 |
PaxDbi | Q8IUD6 |
PeptideAtlasi | Q8IUD6 |
PRIDEi | Q8IUD6 |
ProteomicsDBi | 24321 70552 [Q8IUD6-1] 70553 [Q8IUD6-2] |
PTM databases
iPTMneti | Q8IUD6 |
PhosphoSitePlusi | Q8IUD6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000181481, Expressed in layer of synovial tissue and 216 other tissues |
ExpressionAtlasi | Q8IUD6, baseline and differential |
Genevisiblei | Q8IUD6, HS |
Organism-specific databases
HPAi | ENSG00000181481, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (PubMed:31006531).
Interacts (homodimer) with DDX58 (double-stranded RNA-bound oligomeric form); involved in both DDX58 ubiquitination, oligomerization into filaments associated with viral RNAs and the bridging of these filaments (PubMed:19017631, PubMed:19484123, PubMed:23950712, PubMed:28469175, PubMed:31006531).
Interacts with UBE2D3 and UBE2N; E2 ubiquitin ligases involved in RNF135-mediated ubiquitination of DDX58 and activation of the RIG-I signaling pathway (PubMed:28469175).
Interacts with PCBP2 (PubMed:19881509).
6 PublicationsBinary interactionsi
Hide detailsQ8IUD6
GO - Molecular functioni
- identical protein binding Source: IntAct
- RIG-I binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 124009, 23 interactors |
IntActi | Q8IUD6, 17 interactors |
STRINGi | 9606.ENSP00000328340 |
Miscellaneous databases
RNActi | Q8IUD6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 241 – 432 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 192 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 121 – 156 | Sequence analysisAdd BLAST | 36 | |
Coiled coili | 191 – 216 | Sequence analysisAdd BLAST | 26 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 21 – 63 | RING-typePROSITE-ProRule annotationAdd BLAST | 43 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00830000128449 |
HOGENOMi | CLU_032372_2_0_1 |
InParanoidi | Q8IUD6 |
OMAi | PQSYPWS |
OrthoDBi | 751018at2759 |
PhylomeDBi | Q8IUD6 |
TreeFami | TF351089 |
Family and domain databases
CDDi | cd12902, SPRY_PRY_RNF135, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR042723, RNF135_SPRY_PRY_dom IPR003877, SPRY_dom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00622, SPRY, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGLGLGSAV PVWLAEDDLG CIICQGLLDW PATLPCGHSF CRHCLEALWG
60 70 80 90 100
ARDARRWACP TCRQGAAQQP HLRKNTLLQD LADKYRRAAR EIQAGSDPAH
110 120 130 140 150
CPCPGSSSLS SAAARPRRRP ELQRVAVEKS ITEVAQELTE LVEHLVDIVR
160 170 180 190 200
SLQNQRPLSE SGPDNELSIL GKAFSSGVDL SMASPKLVTS DTAAGKIRDI
210 220 230 240 250
LHDLEEIQEK LQESVTWKEA PEAQMQGELL EAPSSSSCPL PDQSHPALRR
260 270 280 290 300
ASRFAQWAIH PTFNLKSLSC SLEVSKDSRT VTVSHRPQPY RWSCERFSTS
310 320 330 340 350
QVLCSQALSS GKHYWEVDTR NCSHWAVGVA SWEMSRDQVL GRTMDSCCVE
360 370 380 390 400
WKGTSQLSAW HMVKETVLGS DRPGVVGIWL NLEEGKLAFY SVDNQEKLLY
410 420 430
ECTISASSPL YPAFWLYGLH PGNYLIIKQV KV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C3H8 | H7C3H8_HUMAN | E3 ubiquitin-protein ligase RNF135 | RNF135 | 162 | Annotation score: | ||
J3QS68 | J3QS68_HUMAN | E3 ubiquitin-protein ligase RNF135 | RNF135 | 158 | Annotation score: | ||
K7EQF1 | K7EQF1_HUMAN | E3 ubiquitin-protein ligase RNF135 | RNF135 | 86 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 274 | V → G in CAD43140 (PubMed:12696059).Curated | 1 | |
Sequence conflicti | 274 | V → G in AAT06743 (PubMed:15334068).Curated | 1 | |
Sequence conflicti | 293 | S → N in CAD43140 (PubMed:12696059).Curated | 1 | |
Sequence conflicti | 293 | S → N in AAT06743 (PubMed:15334068).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031165 | 71 | H → Q1 PublicationCorresponds to variant dbSNP:rs7225888Ensembl. | 1 | |
Natural variantiVAR_031166 | 108 | S → P1 PublicationCorresponds to variant dbSNP:rs7211440Ensembl. | 1 | |
Natural variantiVAR_063495 | 115 | R → K1 PublicationCorresponds to variant dbSNP:rs111902263EnsemblClinVar. | 1 | |
Natural variantiVAR_037652 | 286 | R → H Found in an individual with overgrowth, learning disability and dysmorphic features; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs121918162EnsemblClinVar. | 1 | |
Natural variantiVAR_063496 | 415 | W → C1 PublicationCorresponds to variant dbSNP:rs61749868EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023785 | 173 – 210 | AFSSG…EIQEK → ENSWKPRLPPHAHCLTRATL HSGELLGLLSGPSIQPLT in isoform 2. 2 PublicationsAdd BLAST | 38 | |
Alternative sequenceiVSP_023786 | 211 – 432 | Missing in isoform 2. 2 PublicationsAdd BLAST | 222 | |
Alternative sequenceiVSP_045359 | 228 – 286 | ELLEA…TVSHR → SLLPRLECSGTITAASISQA QENSWKPRLPPHAHCLTRAT LHSGELLGLLSGPSIQPLT in isoform 3. 1 PublicationAdd BLAST | 59 | |
Alternative sequenceiVSP_045360 | 287 – 432 | Missing in isoform 3. 1 PublicationAdd BLAST | 146 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ496729 mRNA Translation: CAD43140.1 AY598332 mRNA Translation: AAT06743.1 AB470605 mRNA Translation: BAG84604.1 AK122646 mRNA Translation: BAG53638.1 AK312979 mRNA Translation: BAG35816.1 AC138207 Genomic DNA No translation available. CH471147 Genomic DNA Translation: EAW80286.1 BC005084 mRNA Translation: AAH05084.1 BC082262 mRNA No translation available. BC126420 mRNA Translation: AAI26421.1 BC126422 mRNA Translation: AAI26423.1 |
CCDSi | CCDS11262.1 [Q8IUD6-1] CCDS11263.1 [Q8IUD6-2] CCDS54104.1 [Q8IUD6-3] |
RefSeqi | NP_001171921.1, NM_001184992.1 [Q8IUD6-3] NP_115698.3, NM_032322.3 [Q8IUD6-1] NP_922921.1, NM_197939.1 [Q8IUD6-2] |
Genome annotation databases
Ensembli | ENST00000324689; ENSP00000323693; ENSG00000181481 [Q8IUD6-2] ENST00000328381; ENSP00000328340; ENSG00000181481 [Q8IUD6-1] ENST00000535306; ENSP00000440470; ENSG00000181481 [Q8IUD6-3] |
GeneIDi | 84282 |
KEGGi | hsa:84282 |
UCSCi | uc002hfz.4, human [Q8IUD6-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Leiden Open Variation Database Ring finger protein 135 (RNF135) |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ496729 mRNA Translation: CAD43140.1 AY598332 mRNA Translation: AAT06743.1 AB470605 mRNA Translation: BAG84604.1 AK122646 mRNA Translation: BAG53638.1 AK312979 mRNA Translation: BAG35816.1 AC138207 Genomic DNA No translation available. CH471147 Genomic DNA Translation: EAW80286.1 BC005084 mRNA Translation: AAH05084.1 BC082262 mRNA No translation available. BC126420 mRNA Translation: AAI26421.1 BC126422 mRNA Translation: AAI26423.1 |
CCDSi | CCDS11262.1 [Q8IUD6-1] CCDS11263.1 [Q8IUD6-2] CCDS54104.1 [Q8IUD6-3] |
RefSeqi | NP_001171921.1, NM_001184992.1 [Q8IUD6-3] NP_115698.3, NM_032322.3 [Q8IUD6-1] NP_922921.1, NM_197939.1 [Q8IUD6-2] |
3D structure databases
SMRi | Q8IUD6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 124009, 23 interactors |
IntActi | Q8IUD6, 17 interactors |
STRINGi | 9606.ENSP00000328340 |
PTM databases
iPTMneti | Q8IUD6 |
PhosphoSitePlusi | Q8IUD6 |
Polymorphism and mutation databases
BioMutai | RNF135 |
DMDMi | 269849639 |
Proteomic databases
EPDi | Q8IUD6 |
jPOSTi | Q8IUD6 |
MassIVEi | Q8IUD6 |
MaxQBi | Q8IUD6 |
PaxDbi | Q8IUD6 |
PeptideAtlasi | Q8IUD6 |
PRIDEi | Q8IUD6 |
ProteomicsDBi | 24321 70552 [Q8IUD6-1] 70553 [Q8IUD6-2] |
Protocols and materials databases
Antibodypediai | 15187, 151 antibodies |
Genome annotation databases
Ensembli | ENST00000324689; ENSP00000323693; ENSG00000181481 [Q8IUD6-2] ENST00000328381; ENSP00000328340; ENSG00000181481 [Q8IUD6-1] ENST00000535306; ENSP00000440470; ENSG00000181481 [Q8IUD6-3] |
GeneIDi | 84282 |
KEGGi | hsa:84282 |
UCSCi | uc002hfz.4, human [Q8IUD6-1] |
Organism-specific databases
CTDi | 84282 |
DisGeNETi | 84282 |
EuPathDBi | HostDB:ENSG00000181481.13 |
GeneCardsi | RNF135 |
HGNCi | HGNC:21158, RNF135 |
HPAi | ENSG00000181481, Low tissue specificity |
MalaCardsi | RNF135 |
MIMi | 611358, gene |
neXtProti | NX_Q8IUD6 |
OpenTargetsi | ENSG00000181481 |
Orphaneti | 137634, Overgrowth-macrocephaly-facial dysmorphism syndrome |
PharmGKBi | PA134978537 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00830000128449 |
HOGENOMi | CLU_032372_2_0_1 |
InParanoidi | Q8IUD6 |
OMAi | PQSYPWS |
OrthoDBi | 751018at2759 |
PhylomeDBi | Q8IUD6 |
TreeFami | TF351089 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q8IUD6 |
Reactomei | R-HSA-168928, DDX58/IFIH1-mediated induction of interferon-alpha/beta R-HSA-5689896, Ovarian tumor domain proteases R-HSA-918233, TRAF3-dependent IRF activation pathway R-HSA-933541, TRAF6 mediated IRF7 activation R-HSA-933542, TRAF6 mediated NF-kB activation R-HSA-933543, NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling |
Miscellaneous databases
BioGRID-ORCSi | 84282, 5 hits in 845 CRISPR screens |
ChiTaRSi | RNF135, human |
GeneWikii | RNF135 |
GenomeRNAii | 84282 |
Pharosi | Q8IUD6, Tbio |
PROi | PR:Q8IUD6 |
RNActi | Q8IUD6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000181481, Expressed in layer of synovial tissue and 216 other tissues |
ExpressionAtlasi | Q8IUD6, baseline and differential |
Genevisiblei | Q8IUD6, HS |
Family and domain databases
CDDi | cd12902, SPRY_PRY_RNF135, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR042723, RNF135_SPRY_PRY_dom IPR003877, SPRY_dom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00622, SPRY, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00589, PRY, 1 hit SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RN135_HUMAN | |
Accessioni | Q8IUD6Primary (citable) accession number: Q8IUD6 Secondary accession number(s): A0AVM5 Q9BSE9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | November 24, 2009 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations