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UniProtKB - Q8IUD6 (RN135_HUMAN)

Entry version 156 (29 Sep 2021)
Sequence version 2 (24 Nov 2009)
Previous versions | rss
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Protein

E3 ubiquitin-protein ligase RNF135

Gene

RNF135

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

E2-dependent E3 ubiquitin-protein ligase that functions as a RIG-I/DDX58 coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response (PubMed:19017631, PubMed:19484123, PubMed:21147464, PubMed:23950712, PubMed:28469175, PubMed:31006531).

Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway (PubMed:19017631, PubMed:21147464, PubMed:28469175, PubMed:31006531).

Through a ubiquitin-independent parallel mechanism, which consists in bridging RIG-I/DDX58 filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway (PubMed:31006531).

This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway (PubMed:28469175, PubMed:31006531).

1 Publication6 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri21 – 63RING-typePROSITE-ProRule annotationAdd BLAST43

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processImmunity, Innate immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q8IUD6

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-168928, DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5689896, Ovarian tumor domain proteases
R-HSA-918233, TRAF3-dependent IRF activation pathway
R-HSA-933541, TRAF6 mediated IRF7 activation
R-HSA-933542, TRAF6 mediated NF-kB activation
R-HSA-933543, NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10
R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF135Curated (EC:2.3.2.271 Publication)
Alternative name(s):
RIG-I E3 ubiquitin ligase1 Publication
Short name:
REUL1 Publication
RING finger protein 135
RING finger protein leading to RIG-I activation1 Publication
Short name:
Riplet1 Publication
RING-type E3 ubiquitin transferase RNF135Curated
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:RNF135Imported
ORF Names:L13
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:21158, RNF135

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		611358, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q8IUD6

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000181481

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi16 – 18EDD → AAA: Prevents degradation by hepatitis C virus NS3/NS4A. 1 Publication3
Mutagenesisi21C → A: Loss of function in RIG-I signaling pathway; when associated with A-24. 1 Publication1
Mutagenesisi24C → A: Loss of function in RIG-I signaling pathway; when associated with A-21. 1 Publication1

Keywords - Diseasei

Disease variant

Organism-specific databases

DisGeNET

More...DisGeNETi		84282

MalaCards human disease database

More...MalaCardsi		RNF135

Open Targets

More...OpenTargetsi		ENSG00000181481

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...Orphaneti		137634, Overgrowth-macrocephaly-facial dysmorphism syndrome

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA134978537

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q8IUD6, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		RNF135

Domain mapping of disease mutations (DMDM)

More...DMDMi		269849639

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002805571 – 432E3 ubiquitin-protein ligase RNF135Add BLAST432

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

(Microbial infection) Cleaved and inactivated by hepatitis C virus NS3/NS4A.1 Publication

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q8IUD6

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q8IUD6

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q8IUD6

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q8IUD6

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q8IUD6

PeptideAtlas

More...PeptideAtlasi		Q8IUD6

PRoteomics IDEntifications database

More...PRIDEi		Q8IUD6

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		24321
70552 [Q8IUD6-1]
70553 [Q8IUD6-2]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q8IUD6, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q8IUD6

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q8IUD6

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000181481, Expressed in layer of synovial tissue and 217 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q8IUD6, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q8IUD6, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000181481, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer (PubMed:31006531).

Interacts (homodimer) with DDX58 (double-stranded RNA-bound oligomeric form); involved in both DDX58 ubiquitination, oligomerization into filaments associated with viral RNAs and the bridging of these filaments (PubMed:19017631, PubMed:19484123, PubMed:23950712, PubMed:28469175, PubMed:31006531).

Interacts with UBE2D3 and UBE2N; E2 ubiquitin ligases involved in RNF135-mediated ubiquitination of DDX58 and activation of the RIG-I signaling pathway (PubMed:28469175).

Interacts with PCBP2 (PubMed:19881509).

6 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		124009, 23 interactors

Protein interaction database and analysis system

More...IntActi		Q8IUD6, 17 interactors

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000328340

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q8IUD6, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q8IUD6

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini241 – 432B30.2/SPRYPROSITE-ProRule annotationAdd BLAST192

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni95 – 121DisorderedSequence analysisAdd BLAST27

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili121 – 156Sequence analysisAdd BLAST36
Coiled coili191 – 216Sequence analysisAdd BLAST26

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The B30.2/SPRY domain mediates the interaction with the substrate RIG-I/DDX58.2 Publications
The coiled-coil domains mediate homodimerization and the bridging of viral RNA-associated RIG-I/DDX58 filaments.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 63RING-typePROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2177, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00830000128449

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_032372_2_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q8IUD6

Identification of Orthologs from Complete Genome Data

More...OMAi		DCLKGLW

Database of Orthologous Groups

More...OrthoDBi		751018at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q8IUD6

TreeFam database of animal gene trees

More...TreeFami		TF351089

Family and domain databases

Conserved Domains Database

More...CDDi		cd12902, SPRY_PRY_RNF135, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.60.120.920, 1 hit
3.30.40.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001870, B30.2/SPRY
IPR043136, B30.2/SPRY_sf
IPR003879, Butyrophylin_SPRY
IPR013320, ConA-like_dom_sf
IPR006574, PRY
IPR042723, RNF135_SPRY_PRY_dom
IPR003877, SPRY_dom
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD
IPR017907, Znf_RING_CS

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00622, SPRY, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR01407, BUTYPHLNCDUF

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00589, PRY, 1 hit
SM00184, RING, 1 hit
SM00449, SPRY, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF49899, SSF49899, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50188, B302_SPRY, 1 hit
PS00518, ZF_RING_1, 1 hit
PS50089, ZF_RING_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IUD6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGLGLGSAV PVWLAEDDLG CIICQGLLDW PATLPCGHSF CRHCLEALWG 
        60         70         80         90        100
ARDARRWACP TCRQGAAQQP HLRKNTLLQD LADKYRRAAR EIQAGSDPAH 
       110        120        130        140        150
CPCPGSSSLS SAAARPRRRP ELQRVAVEKS ITEVAQELTE LVEHLVDIVR 
       160        170        180        190        200
SLQNQRPLSE SGPDNELSIL GKAFSSGVDL SMASPKLVTS DTAAGKIRDI 
       210        220        230        240        250
LHDLEEIQEK LQESVTWKEA PEAQMQGELL EAPSSSSCPL PDQSHPALRR 
       260        270        280        290        300
ASRFAQWAIH PTFNLKSLSC SLEVSKDSRT VTVSHRPQPY RWSCERFSTS 
       310        320        330        340        350
QVLCSQALSS GKHYWEVDTR NCSHWAVGVA SWEMSRDQVL GRTMDSCCVE 
       360        370        380        390        400
WKGTSQLSAW HMVKETVLGS DRPGVVGIWL NLEEGKLAFY SVDNQEKLLY 
       410        420        430 
ECTISASSPL YPAFWLYGLH PGNYLIIKQV KV
Length:432
Mass (Da):47,888
Last modified:November 24, 2009 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i488F05406996311D
GO
Isoform 2 (identifier: Q8IUD6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     173-210: AFSSGVDLSM...LHDLEEIQEK → ENSWKPRLPP...LSGPSIQPLT
     211-432: Missing.

Show »
Length:210
Mass (Da):22,891
Checksum:i8E57034E5F6DF444
GO
Isoform 3 (identifier: Q8IUD6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-286: ELLEAPSSSS...DSRTVTVSHR → SLLPRLECSG...LSGPSIQPLT
     287-432: Missing.

Show »
Length:286
Mass (Da):30,989
Checksum:iC08F98D2BBF6544E
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C3H8H7C3H8_HUMAN
E3 ubiquitin-protein ligase RNF135
RNF135
162Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QS68J3QS68_HUMAN
E3 ubiquitin-protein ligase RNF135
RNF135
158Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
K7EQF1K7EQF1_HUMAN
E3 ubiquitin-protein ligase RNF135
RNF135
86Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti274V → G in CAD43140 (PubMed:12696059).Curated1
Sequence conflicti274V → G in AAT06743 (PubMed:15334068).Curated1
Sequence conflicti293S → N in CAD43140 (PubMed:12696059).Curated1
Sequence conflicti293S → N in AAT06743 (PubMed:15334068).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_03116571H → Q1 PublicationCorresponds to variant dbSNP:rs7225888Ensembl.1
Natural variantiVAR_031166108S → P1 PublicationCorresponds to variant dbSNP:rs7211440Ensembl.1
Natural variantiVAR_063495115R → K1 PublicationCorresponds to variant dbSNP:rs111902263EnsemblClinVar.1
Natural variantiVAR_037652286R → H Found in an individual with overgrowth, learning disability and dysmorphic features; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs121918162EnsemblClinVar.1
Natural variantiVAR_063496415W → C1 PublicationCorresponds to variant dbSNP:rs61749868EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_023785173 – 210AFSSG…EIQEK → ENSWKPRLPPHAHCLTRATL HSGELLGLLSGPSIQPLT in isoform 2. 2 PublicationsAdd BLAST38
Alternative sequenceiVSP_023786211 – 432Missing in isoform 2. 2 PublicationsAdd BLAST222
Alternative sequenceiVSP_045359228 – 286ELLEA…TVSHR → SLLPRLECSGTITAASISQA QENSWKPRLPPHAHCLTRAT LHSGELLGLLSGPSIQPLT in isoform 3. 1 PublicationAdd BLAST59
Alternative sequenceiVSP_045360287 – 432Missing in isoform 3. 1 PublicationAdd BLAST146

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AJ496729 mRNA Translation: CAD43140.1
AY598332 mRNA Translation: AAT06743.1
AB470605 mRNA Translation: BAG84604.1
AK122646 mRNA Translation: BAG53638.1
AK312979 mRNA Translation: BAG35816.1
AC138207 Genomic DNA No translation available.
CH471147 Genomic DNA Translation: EAW80286.1
BC005084 mRNA Translation: AAH05084.1
BC082262 mRNA No translation available.
BC126420 mRNA Translation: AAI26421.1
BC126422 mRNA Translation: AAI26423.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS11262.1 [Q8IUD6-1]
CCDS11263.1 [Q8IUD6-2]
CCDS54104.1 [Q8IUD6-3]

NCBI Reference Sequences

More...RefSeqi		NP_001171921.1, NM_001184992.1 [Q8IUD6-3]
NP_115698.3, NM_032322.3 [Q8IUD6-1]
NP_922921.1, NM_197939.1 [Q8IUD6-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000324689; ENSP00000323693; ENSG00000181481 [Q8IUD6-2]
ENST00000328381; ENSP00000328340; ENSG00000181481 [Q8IUD6-1]
ENST00000535306; ENSP00000440470; ENSG00000181481 [Q8IUD6-3]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		84282

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:84282

UCSC genome browser

More...UCSCi		uc002hfz.4, human [Q8IUD6-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Leiden Open Variation Database

Ring finger protein 135 (RNF135)

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ496729 mRNA Translation: CAD43140.1
AY598332 mRNA Translation: AAT06743.1
AB470605 mRNA Translation: BAG84604.1
AK122646 mRNA Translation: BAG53638.1
AK312979 mRNA Translation: BAG35816.1
AC138207 Genomic DNA No translation available.
CH471147 Genomic DNA Translation: EAW80286.1
BC005084 mRNA Translation: AAH05084.1
BC082262 mRNA No translation available.
BC126420 mRNA Translation: AAI26421.1
BC126422 mRNA Translation: AAI26423.1
CCDSiCCDS11262.1 [Q8IUD6-1]
CCDS11263.1 [Q8IUD6-2]
CCDS54104.1 [Q8IUD6-3]
RefSeqiNP_001171921.1, NM_001184992.1 [Q8IUD6-3]
NP_115698.3, NM_032322.3 [Q8IUD6-1]
NP_922921.1, NM_197939.1 [Q8IUD6-2]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
7JL1electron microscopy3.90B249-432[»]
7JL3electron microscopy4.20B/D/F249-432[»]
SMRiQ8IUD6
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi124009, 23 interactors
IntActiQ8IUD6, 17 interactors
STRINGi9606.ENSP00000328340

PTM databases

GlyGeniQ8IUD6, 1 site, 1 O-linked glycan (1 site)
iPTMnetiQ8IUD6
PhosphoSitePlusiQ8IUD6

Genetic variation databases

BioMutaiRNF135
DMDMi269849639

Proteomic databases

EPDiQ8IUD6
jPOSTiQ8IUD6
MassIVEiQ8IUD6
MaxQBiQ8IUD6
PaxDbiQ8IUD6
PeptideAtlasiQ8IUD6
PRIDEiQ8IUD6
ProteomicsDBi24321
70552 [Q8IUD6-1]
70553 [Q8IUD6-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		15187, 160 antibodies

The DNASU plasmid repository

More...DNASUi		84282

Genome annotation databases

EnsembliENST00000324689; ENSP00000323693; ENSG00000181481 [Q8IUD6-2]
ENST00000328381; ENSP00000328340; ENSG00000181481 [Q8IUD6-1]
ENST00000535306; ENSP00000440470; ENSG00000181481 [Q8IUD6-3]
GeneIDi84282
KEGGihsa:84282
UCSCiuc002hfz.4, human [Q8IUD6-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		84282
DisGeNETi84282

GeneCards: human genes, protein and diseases

More...GeneCardsi		RNF135
HGNCiHGNC:21158, RNF135
HPAiENSG00000181481, Low tissue specificity
MalaCardsiRNF135
MIMi611358, gene
neXtProtiNX_Q8IUD6
OpenTargetsiENSG00000181481
Orphaneti137634, Overgrowth-macrocephaly-facial dysmorphism syndrome
PharmGKBiPA134978537
VEuPathDBiHostDB:ENSG00000181481

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG2177, Eukaryota
GeneTreeiENSGT00830000128449
HOGENOMiCLU_032372_2_0_1
InParanoidiQ8IUD6
OMAiDCLKGLW
OrthoDBi751018at2759
PhylomeDBiQ8IUD6
TreeFamiTF351089

Enzyme and pathway databases

UniPathwayiUPA00143
PathwayCommonsiQ8IUD6
ReactomeiR-HSA-168928, DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5689896, Ovarian tumor domain proteases
R-HSA-918233, TRAF3-dependent IRF activation pathway
R-HSA-933541, TRAF6 mediated IRF7 activation
R-HSA-933542, TRAF6 mediated NF-kB activation
R-HSA-933543, NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10
R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		84282, 6 hits in 1048 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		RNF135, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		RNF135

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		84282
PharosiQ8IUD6, Tbio

Protein Ontology

More...PROi		PR:Q8IUD6
RNActiQ8IUD6, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000181481, Expressed in layer of synovial tissue and 217 other tissues
ExpressionAtlasiQ8IUD6, baseline and differential
GenevisibleiQ8IUD6, HS

Family and domain databases

CDDicd12902, SPRY_PRY_RNF135, 1 hit
Gene3Di2.60.120.920, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870, B30.2/SPRY
IPR043136, B30.2/SPRY_sf
IPR003879, Butyrophylin_SPRY
IPR013320, ConA-like_dom_sf
IPR006574, PRY
IPR042723, RNF135_SPRY_PRY_dom
IPR003877, SPRY_dom
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD
IPR017907, Znf_RING_CS
PfamiView protein in Pfam
PF00622, SPRY, 1 hit
PRINTSiPR01407, BUTYPHLNCDUF
SMARTiView protein in SMART
SM00589, PRY, 1 hit
SM00184, RING, 1 hit
SM00449, SPRY, 1 hit
SUPFAMiSSF49899, SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188, B302_SPRY, 1 hit
PS00518, ZF_RING_1, 1 hit
PS50089, ZF_RING_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRN135_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8IUD6
Secondary accession number(s): A0AVM5
, B2R7G9, B6ZLM5, F5GX60, Q9BSE9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 24, 2009
Last modified: September 29, 2021
This is version 156 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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