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Protein

m7GpppN-mRNA hydrolase

Gene

DCP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:12417715, PubMed:12218187, PubMed:12923261, PubMed:21070968). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:12486012, PubMed:12923261, PubMed:21070968). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed:14527413). Plays a role in replication-dependent histone mRNA degradation (PubMed:18172165). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degration of their transcripts (PubMed:26098573).8 Publications

Catalytic activityi

5'-(N7-methylguanosine 5'-triphospho)-[mRNA] + H2O = N7-methylguanosine 5'-diphosphate + 5'-phospho-[mRNA].3 Publications

Cofactori

Mn2+1 Publication, Mg2+1 PublicationNote: Mn2+ ion is required for highest activity. Can also utilize magnesium ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144ManganeseBy similarity1
Metal bindingi148ManganeseBy similarity1

GO - Molecular functioni

  • 5'-3' exoribonuclease activity Source: BHF-UCL
  • exoribonuclease activity, producing 5'-phosphomonoesters Source: Reactome
  • m7G(5')pppN diphosphatase activity Source: UniProtKB
  • manganese ion binding Source: InterPro
  • telomerase RNA binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionHydrolase, RNA-binding
Biological processNonsense-mediated mRNA decay
LigandManganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.6.1.62 2681
ReactomeiR-HSA-380994 ATF4 activates genes
R-HSA-430039 mRNA decay by 5' to 3' exoribonuclease
R-HSA-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-HSA-450513 Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA
R-HSA-450604 KSRP (KHSRP) binds and destabilizes mRNA

Names & Taxonomyi

Protein namesi
Recommended name:
m7GpppN-mRNA hydrolaseCurated (EC:3.6.1.623 Publications)
Alternative name(s):
Nucleoside diphosphate-linked moiety X motif 20
Short name:
Nudix motif 20
mRNA-decapping enzyme 2
Short name:
hDpc
Gene namesi
Name:DCP2
Synonyms:NUDT20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000172795.15
HGNCiHGNC:24452 DCP2
MIMi609844 gene
neXtProtiNX_Q8IU60

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi147E → Q: Loss of decapping activity; when associated with Q-148. 1
Mutagenesisi148E → Q: Strongly reduced decapping activity. 1 Publication1
Mutagenesisi249S → A: Leads to the accumulation of autophagosomes under normal growth conditions. 1 Publication1
Mutagenesisi249S → D: Leads to reduced autophagosome formation under autophagy-inducing conditions. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000172795
PharmGKBiPA134898646

Polymorphism and mutation databases

BioMutaiDCP2
DMDMi269849560

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000570511 – 420m7GpppN-mRNA hydrolaseAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei246PhosphoserineCombined sources1
Modified residuei247PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei284PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at ser-249 in a MTOR-dependent manner (PubMed:26098573).

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IU60
MaxQBiQ8IU60
PaxDbiQ8IU60
PeptideAtlasiQ8IU60
PRIDEiQ8IU60
ProteomicsDBi70509
70510 [Q8IU60-2]

PTM databases

iPTMnetiQ8IU60
PhosphoSitePlusiQ8IU60

Expressioni

Tissue specificityi

Expressed in brain and testis. Not detected in heart (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000172795 Expressed in 232 organ(s), highest expression level in chorionic villus
CleanExiHS_DCP2
ExpressionAtlasiQ8IU60 baseline and differential
GenevisibleiQ8IU60 HS

Organism-specific databases

HPAiCAB011677
HPA057676

Interactioni

Subunit structurei

Found in a mRNA decay complex with LSM1, LSM3, LSM4, EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, CNOT6, UPF1, UPF2 and UPF3B (PubMed:12417715, PubMed:12515382, PubMed:14527413). Forms a complex with DCP1A, EDC3, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC4/HEDLS (PubMed:15231747, PubMed:15067023, PubMed:16364915). Interacts with DPC1B (PubMed:15231747). Interacts (via N-terminus and C-terminus) with TRIM21 (via N-terminus and C-terminus) (PubMed:18361920). Associates with polysomes (PubMed:12218187). Interacts with LIMD1, WTIP and AJUBA (PubMed:20616046). Interacts with DDX17 in an RNA-dependent manner (PubMed:21876179). Interacts with ZC3HAV1 (PubMed:21876179). Interacts with APOBEC3G in an RNA-dependent manner (PubMed:16699599). Interacts with ZFP36L1 (via N-terminus) (PubMed:15687258). Interacts with NBDY (PubMed:27918561).13 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi127944, 68 interactors
CORUMiQ8IU60
DIPiDIP-31126N
IntActiQ8IU60, 16 interactors
STRINGi9606.ENSP00000373715

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8IU60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 226Nudix hydrolasePROSITE-ProRule annotationAdd BLAST132

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi129 – 150Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. DCP2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2937 Eukaryota
COG0494 LUCA
GeneTreeiENSGT00390000018878
HOGENOMiHOG000005974
HOVERGENiHBG051321
InParanoidiQ8IU60
KOiK12613
OrthoDBiEOG091G05Q0
PhylomeDBiQ8IU60
TreeFamiTF314180

Family and domain databases

Gene3Di1.10.10.1050, 1 hit
InterProiView protein in InterPro
IPR007722 DCP2_BoxA
IPR036189 DCP2_BoxA_sf
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF05026 DCP2, 1 hit
PF00293 NUDIX, 1 hit
SMARTiView protein in SMART
SM01125 DCP2, 1 hit
SUPFAMiSSF140586 SSF140586, 1 hit
SSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IU60-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METKRVEIPG SVLDDLCSRF ILHIPSEERD NAIRVCFQIE LAHWFYLDFY
60 70 80 90 100
MQNTPGLPQC GIRDFAKAVF SHCPFLLPQG EDVEKVLDEW KEYKMGVPTY
110 120 130 140 150
GAIILDETLE NVLLVQGYLA KSGWGFPKGK VNKEEAPHDC AAREVFEETG
160 170 180 190 200
FDIKDYICKD DYIELRINDQ LARLYIIPGI PKDTKFNPKT RREIRNIEWF
210 220 230 240 250
SIEKLPCHRN DMTPKSKLGL APNKFFMAIP FIRPLRDWLS RRFGDSSDSD
260 270 280 290 300
NGFSSTGSTP AKPTVEKLSR TKFRHSQQLF PDGSPGDQWV KHRQPLQQKP
310 320 330 340 350
YNNHSEMSDL LKGKNQSMRG NGRKQYQDSP NQKKRTNGLQ PAKQQNSLMK
360 370 380 390 400
CEKKLHPRKL QDNFETDAVY DLPSSSEDQL LEHAEGQPVA CNGHCKFPFS
410 420
SRAFLSFKFD HNAIMKILDL
Length:420
Mass (Da):48,423
Last modified:September 12, 2018 - v3
Checksum:iC0FA503A0C797967
GO
Isoform 2 (identifier: Q8IU60-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     315-349: Missing.

Note: No experimental confirmation available.
Show »
Length:385
Mass (Da):44,409
Checksum:i85D8725238E3C367
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y9T5H0Y9T5_HUMAN
m7GpppN-mRNA hydrolase
DCP2
402Annotation score:
D6RCL0D6RCL0_HUMAN
m7GpppN-mRNA hydrolase
DCP2
74Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103I → V in BAC03479 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05952816L → F1 PublicationCorresponds to variant dbSNP:rs33555Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012908315 – 349Missing in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY146650 mRNA Translation: AAN62762.1
AY135173 mRNA Translation: AAN08884.1
AK090564 mRNA Translation: BAC03479.1
AC008536 Genomic DNA No translation available.
BC045596 mRNA Translation: AAH45596.1
BC064593 mRNA Translation: AAH64593.1
CCDSiCCDS34210.1 [Q8IU60-1]
CCDS56377.1 [Q8IU60-2]
RefSeqiNP_001229306.1, NM_001242377.1 [Q8IU60-2]
NP_689837.2, NM_152624.5 [Q8IU60-1]
UniGeneiHs.443875

Genome annotation databases

EnsembliENST00000389063; ENSP00000373715; ENSG00000172795 [Q8IU60-1]
ENST00000515408; ENSP00000425770; ENSG00000172795 [Q8IU60-2]
GeneIDi167227
KEGGihsa:167227
UCSCiuc003kqh.4 human [Q8IU60-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY146650 mRNA Translation: AAN62762.1
AY135173 mRNA Translation: AAN08884.1
AK090564 mRNA Translation: BAC03479.1
AC008536 Genomic DNA No translation available.
BC045596 mRNA Translation: AAH45596.1
BC064593 mRNA Translation: AAH64593.1
CCDSiCCDS34210.1 [Q8IU60-1]
CCDS56377.1 [Q8IU60-2]
RefSeqiNP_001229306.1, NM_001242377.1 [Q8IU60-2]
NP_689837.2, NM_152624.5 [Q8IU60-1]
UniGeneiHs.443875

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MP0X-ray1.63D95-260[»]
ProteinModelPortaliQ8IU60
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127944, 68 interactors
CORUMiQ8IU60
DIPiDIP-31126N
IntActiQ8IU60, 16 interactors
STRINGi9606.ENSP00000373715

PTM databases

iPTMnetiQ8IU60
PhosphoSitePlusiQ8IU60

Polymorphism and mutation databases

BioMutaiDCP2
DMDMi269849560

Proteomic databases

EPDiQ8IU60
MaxQBiQ8IU60
PaxDbiQ8IU60
PeptideAtlasiQ8IU60
PRIDEiQ8IU60
ProteomicsDBi70509
70510 [Q8IU60-2]

Protocols and materials databases

DNASUi167227
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000389063; ENSP00000373715; ENSG00000172795 [Q8IU60-1]
ENST00000515408; ENSP00000425770; ENSG00000172795 [Q8IU60-2]
GeneIDi167227
KEGGihsa:167227
UCSCiuc003kqh.4 human [Q8IU60-1]

Organism-specific databases

CTDi167227
EuPathDBiHostDB:ENSG00000172795.15
GeneCardsiDCP2
HGNCiHGNC:24452 DCP2
HPAiCAB011677
HPA057676
MIMi609844 gene
neXtProtiNX_Q8IU60
OpenTargetsiENSG00000172795
PharmGKBiPA134898646
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2937 Eukaryota
COG0494 LUCA
GeneTreeiENSGT00390000018878
HOGENOMiHOG000005974
HOVERGENiHBG051321
InParanoidiQ8IU60
KOiK12613
OrthoDBiEOG091G05Q0
PhylomeDBiQ8IU60
TreeFamiTF314180

Enzyme and pathway databases

BRENDAi3.6.1.62 2681
ReactomeiR-HSA-380994 ATF4 activates genes
R-HSA-430039 mRNA decay by 5' to 3' exoribonuclease
R-HSA-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-HSA-450513 Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA
R-HSA-450604 KSRP (KHSRP) binds and destabilizes mRNA

Miscellaneous databases

ChiTaRSiDCP2 human
GeneWikiiDCP2
GenomeRNAii167227
PROiPR:Q8IU60
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172795 Expressed in 232 organ(s), highest expression level in chorionic villus
CleanExiHS_DCP2
ExpressionAtlasiQ8IU60 baseline and differential
GenevisibleiQ8IU60 HS

Family and domain databases

Gene3Di1.10.10.1050, 1 hit
InterProiView protein in InterPro
IPR007722 DCP2_BoxA
IPR036189 DCP2_BoxA_sf
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF05026 DCP2, 1 hit
PF00293 NUDIX, 1 hit
SMARTiView protein in SMART
SM01125 DCP2, 1 hit
SUPFAMiSSF140586 SSF140586, 1 hit
SSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDCP2_HUMAN
AccessioniPrimary (citable) accession number: Q8IU60
Secondary accession number(s): C9J778
, Q6P2D4, Q7Z5W5, Q8NBG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: September 12, 2018
Last modified: November 7, 2018
This is version 149 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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