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Entry version 102 (08 May 2019)
Sequence version 1 (01 Mar 2003)
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Protein

Ras guanine nucleotide exchange factor C

Gene

gefC

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Promotes the exchange of Ras-bound GDP by GTP.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-DDI-171007 p38MAPK events
R-DDI-193648 NRAGE signals death through JNK
R-DDI-194840 Rho GTPase cycle
R-DDI-5673001 RAF/MAP kinase cascade

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ras guanine nucleotide exchange factor C
Alternative name(s):
RasGEF domain-containing protein C
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:gefC
Synonyms:racGEF, rasGEF
ORF Names:DDB_G0282381
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDictyostelium discoideum (Slime mold)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri44689 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002195 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

Dictyostelium discoideum online informatics resource

More...
dictyBasei
DDB_G0282381 gefC

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003844611 – 1457Ras guanine nucleotide exchange factor CAdd BLAST1457

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q8IS20

PRoteomics IDEntifications database

More...
PRIDEi
Q8IS20

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed during development; especially between 8 and 14 hours of development.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
44689.DDB0215004

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati1 – 55RCC1 1Add BLAST55
Repeati351 – 401RCC1 2Add BLAST51
Repeati432 – 483RCC1 3Add BLAST52
Repeati485 – 549RCC1 4Add BLAST65
Repeati590 – 647RCC1 5Add BLAST58
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini650 – 971DHPROSITE-ProRule annotationAdd BLAST322
Domaini989 – 1109N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST121
Domaini1232 – 1454Ras-GEFPROSITE-ProRule annotationAdd BLAST223

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili1138 – 1211Sequence analysisAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi82 – 90Poly-Thr9
Compositional biasi96 – 100Poly-Thr5
Compositional biasi108 – 115Poly-Asn8
Compositional biasi140 – 154Poly-SerAdd BLAST15
Compositional biasi230 – 237Poly-Asp8
Compositional biasi283 – 292Poly-Gln10
Compositional biasi372 – 383Poly-GlnAdd BLAST12
Compositional biasi391 – 401Poly-SerAdd BLAST11
Compositional biasi529 – 532Poly-Gly4
Compositional biasi1151 – 1160Poly-Asn10
Compositional biasi1161 – 1167Poly-Thr7
Compositional biasi1176 – 1208Poly-AsnAdd BLAST33

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1426 Eukaryota
KOG3417 Eukaryota
COG5184 LUCA
ENOG410XR96 LUCA

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q8IS20

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
cd00160 RhoGEF, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.840.10, 2 hits
1.20.900.10, 1 hit
2.130.10.30, 3 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens

The PANTHER Classification System

More...
PANTHERi
PTHR23113 PTHR23113, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
PF00415 RCC1, 3 hits
PF00621 RhoGEF, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00633 RCCNDNSATION

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SM00325 RhoGEF, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48065 SSF48065, 1 hit
SSF48366 SSF48366, 1 hit
SSF50985 SSF50985, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50010 DH_2, 1 hit
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
PS00626 RCC1_2, 2 hits
PS50012 RCC1_3, 4 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8IS20-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVFTFGHGS NGALGLGKIT DDTCPTPQKV NYFTEIDKRV KKVACGSYHT
60 70 80 90 100
VFVTDDEHLY ICGIKKDPKH GTTLFYTSPP PTTTTTTTTT SSTSATTTTT
110 120 130 140 150
NGMVNEKNNN NKNNNGEKIV NSKPESVIIK DGASNTTNDS TSSSSTSTSS
160 170 180 190 200
LSSSLPPTNI ETPRDKKKPP IKLDKGIPHH RSTRELIQRP ESPLLNKLLT
210 220 230 240 250
SKAEMEFKHY ESNEKSKDEM KDNENENEED EDDDDDDSTI RQNEDKESSL
260 270 280 290 300
IDDNESETSL RINKKDAENI TNNIRLIKEL DEQQQQPQQP QQNLKGIMNQ
310 320 330 340 350
PPLPTNDDLD EDPNIHFTPI RISTHFLENS PLNTIKQVAL GNYHIVLLTE
360 370 380 390 400
GGNVWTWGSN SNGQLGYLVD TQQQQQQQLQ QQQSMSSLQP SASSSSPSSS
410 420 430 440 450
SLQTTCTPKM VEIKCVKYIA TGVKNTAALT EWGELYMWGI NEHGELGLGD
460 470 480 490 500
TIDRRTPTRV IRLKNEVVST IACSSTHSAC YTESGKMYVW GQSDETGKIQ
510 520 530 540 550
AIPSPLPIHS YLDREIKSPN GGGVGGFFGG GGKIKQLACG QRCIAVLTQM
560 570 580 590 600
GEVFMWEIPG IPIPLRNALE NHSVRNIVMG NFHLVCLTDS GEVVTIGRNK
610 620 630 640 650
TGQLGRTDAE NEPGIVKQLS FDPDGLNSDE FVVSIAAGEF HSVALVENTP
660 670 680 690 700
KTKLALQLVR MQRNYLRQLN ILNNIYYKSM MSMASPYDPV VLSMMNNGSA
710 720 730 740 750
STLPPSLKGL SGGLPDNANN TIKNGKDKDN HHNGDSNGHH SNGHYHGNGN
760 770 780 790 800
NGNNSITTSN SISSPSLLGS GGTLKSRSAS IATIRGLFGI NHMLHHSQSQ
810 820 830 840 850
SFQEENSTVT EDEIKEIFSD IEALSKLTEK FLSYLDQRMD NWDPVSKVLG
860 870 880 890 900
DIFLDEALMA AYRIYIPYSD SYNTSCMTLF NIRRRNERLS NIIKDCEKKS
910 920 930 940 950
KSFGVKLDQE FVKEIDLKSL LLSPLQNIPR IYILLRELGS DDSGNINPRD
960 970 980 990 1000
IDLINFAATK FQVLLERMNQ NFQFVNAVEI LHCSSNEYGN PQIMGGSLPQ
1010 1020 1030 1040 1050
LVDKLTHHNI SDPNFRDVFL LTFRAFTTPC NLFDLLVETF QRQKHLKNGR
1060 1070 1080 1090 1100
VVNVITSWVV HHFYDFEKDK LSDFTQDPNH NPQLLSEKLE QFIYSEDHQS
1110 1120 1130 1140 1150
VSQIKLQYFS HRKRLESSIN LIDNQKLTQN EITTPPPLQI QNNNQNNNLE
1160 1170 1180 1190 1200
NNNNNNNNNN TTTTTTTPND NINDINNNNN NNNNNNNNNN NNNNNNNNNN
1210 1220 1230 1240 1250
NNNNNNNNLT NSTIKLVVQP PIPINFNLLD SQPIEVAQTL TIMDHHYFAM
1260 1270 1280 1290 1300
IDKREFLGQR WAKNKSPNIQ ISTDHFNRTS QVVVTEILKS KNSKQRSATL
1310 1320 1330 1340 1350
GYFISVAYCC FELNNLSGTA SIIYGLNSAS IQRLKKSWSK LPKESMIAFE
1360 1370 1380 1390 1400
YLDKIVTPMK NYISLRHLMT TIQPPCVPFL GTYLKDLTFI EEGNPSIIGG
1410 1420 1430 1440 1450
LINFYKQRKI AEVIFQIQQH QQVVYSAIRS NPTIKAFLMS SHTFDDKQAQ

KISSEAE
Length:1,457
Mass (Da):163,235
Last modified:March 1, 2003 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i23F79A14AD357AB0
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY160092 Genomic DNA Translation: AAN46872.1
AAFI02000047 Genomic DNA Translation: EAL66050.1

NCBI Reference Sequences

More...
RefSeqi
XP_640241.1, XM_635149.1

Genome annotation databases

Ensembl protists genome annotation project

More...
EnsemblProtistsi
EAL66050; EAL66050; DDB_G0282381

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
8623780

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ddi:DDB_G0282381

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY160092 Genomic DNA Translation: AAN46872.1
AAFI02000047 Genomic DNA Translation: EAL66050.1
RefSeqiXP_640241.1, XM_635149.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi44689.DDB0215004

Proteomic databases

PaxDbiQ8IS20
PRIDEiQ8IS20

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL66050; EAL66050; DDB_G0282381
GeneIDi8623780
KEGGiddi:DDB_G0282381

Organism-specific databases

dictyBaseiDDB_G0282381 gefC

Phylogenomic databases

eggNOGiKOG1426 Eukaryota
KOG3417 Eukaryota
COG5184 LUCA
ENOG410XR96 LUCA
InParanoidiQ8IS20

Enzyme and pathway databases

ReactomeiR-DDI-171007 p38MAPK events
R-DDI-193648 NRAGE signals death through JNK
R-DDI-194840 Rho GTPase cycle
R-DDI-5673001 RAF/MAP kinase cascade

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q8IS20

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.10.840.10, 2 hits
1.20.900.10, 1 hit
2.130.10.30, 3 hits
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
PANTHERiPTHR23113 PTHR23113, 1 hit
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
PF00415 RCC1, 3 hits
PF00621 RhoGEF, 1 hit
PRINTSiPR00633 RCCNDNSATION
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF48366 SSF48366, 1 hit
SSF50985 SSF50985, 2 hits
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
PS00626 RCC1_2, 2 hits
PS50012 RCC1_3, 4 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGEFC_DICDI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8IS20
Secondary accession number(s): Q54RZ8
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 1, 2003
Last modified: May 8, 2019
This is version 102 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
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