UniProtKB - Q8IRG6 (SPT16_DROME)
FACT complex subunit spt16
dre4
Functioni
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is required for expression of Hox genes.
1 PublicationCaution
GO - Molecular functioni
- chromatin binding Source: FlyBase
- nucleosome binding Source: FlyBase
- protein heterodimerization activity Source: CAFA
- protein N-terminus binding Source: CAFA
GO - Biological processi
- DNA repair Source: UniProtKB-KW
- DNA replication Source: UniProtKB-KW
- DNA replication-independent chromatin organization Source: GO_Central
- positive regulation of transcription elongation from RNA polymerase II promoter Source: FlyBase
- regulation of chromatin assembly or disassembly Source: FlyBase
- transcription elongation from RNA polymerase II promoter Source: GO_Central
Keywordsi
Biological process | DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-112382, Formation of RNA Pol II elongation complex R-DME-674695, RNA Polymerase II Pre-transcription Events R-DME-6796648, TP53 Regulates Transcription of DNA Repair Genes R-DME-6804756, Regulation of TP53 Activity through Phosphorylation R-DME-75955, RNA Polymerase II Transcription Elongation |
SignaLinki | Q8IRG6 |
Protein family/group databases
MEROPSi | M24.974 |
Names & Taxonomyi
Protein namesi | Recommended name: FACT complex subunit spt16Alternative name(s): Facilitates chromatin transcription complex subunit SPT16 dSPT16 |
Gene namesi | Name:dre4 Synonyms:spt16 ORF Names:CG1828 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002183, dre4 |
VEuPathDBi | VectorBase:FBgn0002183 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
Note: Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
Cytosol
- cytosol Source: CAFA
Nucleus
- FACT complex Source: FlyBase
- nucleolus Source: UniProtKB
- nucleus Source: FlyBase
Other locations
- polytene chromosome puff Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245174 | 1 – 1083 | FACT complex subunit spt16Add BLAST | 1083 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 437 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8IRG6 |
PRIDEi | Q8IRG6 |
PTM databases
iPTMneti | Q8IRG6 |
Expressioni
Gene expression databases
Bgeei | FBgn0002183, Expressed in imaginal disc and 36 other tissues |
ExpressionAtlasi | Q8IRG6, baseline and differential |
Genevisiblei | Q8IRG6, DM |
Interactioni
Subunit structurei
Component of the FACT complex, a stable heterodimer of dre4/spt16 and Ssrp.
Interacts with TRL/GAGA.
1 PublicationGO - Molecular functioni
- protein heterodimerization activity Source: CAFA
- protein N-terminus binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 63776, 8 interactors |
IntActi | Q8IRG6, 9 interactors |
STRINGi | 7227.FBpp0072743 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 923 – 1083 | DisorderedSequence analysisAdd BLAST | 161 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 466 – 504 | Sequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 938 – 988 | Acidic residuesSequence analysisAdd BLAST | 51 | |
Compositional biasi | 989 – 1022 | Basic and acidic residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 1032 – 1076 | Basic and acidic residuesSequence analysisAdd BLAST | 45 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1189, Eukaryota |
GeneTreei | ENSGT00390000014495 |
HOGENOMi | CLU_004627_0_0_1 |
InParanoidi | Q8IRG6 |
OMAi | HQFFLDG |
PhylomeDBi | Q8IRG6 |
Family and domain databases
CDDi | cd01091, CDC68-like, 1 hit |
DisProti | DP00721 |
Gene3Di | 2.30.29.30, 1 hit 3.40.350.10, 1 hit 3.90.230.10, 1 hit |
IDEALi | IID50095 |
InterProi | View protein in InterPro IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR013719, DUF1747 IPR029148, FACT-Spt16_Nlobe IPR013953, FACT_Spt16 IPR000994, Pept_M24 IPR011993, PH-like_dom_sf IPR040258, Spt16 IPR033825, Spt16_M24 |
PANTHERi | PTHR13980, PTHR13980, 1 hit |
Pfami | View protein in Pfam PF14826, FACT-Spt16_Nlob, 1 hit PF00557, Peptidase_M24, 1 hit PF08512, Rtt106, 1 hit PF08644, SPT16, 1 hit |
SMARTi | View protein in SMART SM01285, FACT-Spt16_Nlob, 1 hit SM01287, Rtt106, 1 hit SM01286, SPT16, 1 hit |
SUPFAMi | SSF55920, SSF55920, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSFVLDKEA FVRRVKRLYT EWRAPSIGHD DALRNLDCIM SIVGVEEDVM
60 70 80 90 100
YSKSMALQLW LLGYELTDTI SVFCSDAVYF LTSKKKIEFL KQTQNITEEG
110 120 130 140 150
FPEINLLVRD RTDKDQGNFE KLIKALQNSK KGKRLGVFAK DAYPGEFSEA
160 170 180 190 200
WKKSLTASKF EHVDISTIIA YLMCPKDESE INNIRKASLV SMDIFNKYLK
210 220 230 240 250
DEIMDIIDSD RKVKHNKLSD GCEAAIGEKK YTSGLDPRLL DMAYPPIIQS
260 270 280 290 300
GGAYSLKFSA VADKNPLHFG VIVCSLGARY KSYCSNISRT FLVNPTEAMQ
310 320 330 340 350
ENYTFLVSVQ EEILKLLVPG TKLCDVYEKT LDFVKKEKPS MVDNLPKSFG
360 370 380 390 400
FAMGLEFREN SIVIGPKCQA LLKKNMVFNL HVGISNLTNP EATDKEGKNY
410 420 430 440 450
ALFIGDTVLV GEQSPASVMT PSKKKIKNVG IFIKDDSDEE DVDDKKTAKE
460 470 480 490 500
DQGTEILGRS KRNAVLESKL RNEINTEEKR KEHQRELAQQ LNERAKDRLA
510 520 530 540 550
RQGNSKEVEK VRKNTVSYKS ISQMPREPEV KELKLYVDKK YETVIMPVFG
560 570 580 590 600
IQVPFHISTI KNISQSVEGE YTYLRINFFH PGATMGRNEG GLYPQPEATF
610 620 630 640 650
VKEVTYRSSN VKEHGEVGAP SANLNNAFRL IKEVQKRFKT REAEEREKED
660 670 680 690 700
LVKQDTLILS QNKGNPKLKD LYIRPNIVTK RMTGSLEAHS NGFRYISVRG
710 720 730 740 750
DKVDILYNNI KSAFFQPCDG EMIILLHFHL KYAIMFGKKK HVDVQFYTEV
760 770 780 790 800
GEITTDLGKH QHMHDRDDLA AEQAERELRH KLKTAFKSFC EKVETMTKSV
810 820 830 840 850
VEFDTPFREL GFPGAPFRST VTLQPTSGSL VNLTEWPPFV ITLDDVELVH
860 870 880 890 900
FERVQFHLRN FDMIFVFKEY NKKVAMVNAI PMNMLDHVKE WLNSCDIRYS
910 920 930 940 950
EGVQSLNWQK IMKTITDDPE GFFEQGGWTF LDPESGSEGE NETAESEEDE
960 970 980 990 1000
AYNPTDAESD EESDEDSEYS EASEDSEESD EDLGSDEESG KDWSDLEREA
1010 1020 1030 1040 1050
AEEDRNHDYA ADDKPRNGKF DSKKHGKSSK HSPSKSSKDK YNSRDKHHSS
1060 1070 1080
SSSGNKSSSK DKDRKRSRDD SRDNGHKSKK SRH
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 345 | L → V in AAB80935 (Ref. 1) Curated | 1 | |
Sequence conflicti | 345 | L → V in AAB80936 (Ref. 1) Curated | 1 | |
Sequence conflicti | 598 | A → R in AAB80935 (Ref. 1) Curated | 1 | |
Sequence conflicti | 598 | A → R in AAB80936 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1057 | S → I in AAB80935 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1057 | S → I in AAB80936 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1080 – 1081 | KS → NA in AAB80935 (Ref. 1) Curated | 2 | |
Sequence conflicti | 1080 – 1081 | KS → NA in AAB80936 (Ref. 1) Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019625 | 1031 | H → HSRRDREEARSSSHSKKHKS NSSSSSSHLKSSSSKHGSSS in isoform A. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF023269 mRNA Translation: AAB80935.1 Frameshift. AF023270 mRNA Translation: AAB80936.1 Frameshift. AE014296 Genomic DNA Translation: AAF47587.2 AE014296 Genomic DNA Translation: AAN11502.2 BT010116 mRNA Translation: AAQ22585.1 AB083008 mRNA Translation: BAC54898.1 |
PIRi | T13928 T13929 |
RefSeqi | NP_476610.2, NM_057262.4 [Q8IRG6-2] NP_728686.2, NM_167922.3 [Q8IRG6-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0072864; FBpp0072743; FBgn0002183 [Q8IRG6-2] FBtr0072865; FBpp0072744; FBgn0002183 [Q8IRG6-1] |
GeneIDi | 38248 |
KEGGi | dme:Dmel_CG1828 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF023269 mRNA Translation: AAB80935.1 Frameshift. AF023270 mRNA Translation: AAB80936.1 Frameshift. AE014296 Genomic DNA Translation: AAF47587.2 AE014296 Genomic DNA Translation: AAN11502.2 BT010116 mRNA Translation: AAQ22585.1 AB083008 mRNA Translation: BAC54898.1 |
PIRi | T13928 T13929 |
RefSeqi | NP_476610.2, NM_057262.4 [Q8IRG6-2] NP_728686.2, NM_167922.3 [Q8IRG6-1] |
3D structure databases
AlphaFoldDBi | Q8IRG6 |
SMRi | Q8IRG6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63776, 8 interactors |
IntActi | Q8IRG6, 9 interactors |
STRINGi | 7227.FBpp0072743 |
Protein family/group databases
MEROPSi | M24.974 |
PTM databases
iPTMneti | Q8IRG6 |
Proteomic databases
PaxDbi | Q8IRG6 |
PRIDEi | Q8IRG6 |
Protocols and materials databases
DNASUi | 38248 |
Genome annotation databases
EnsemblMetazoai | FBtr0072864; FBpp0072743; FBgn0002183 [Q8IRG6-2] FBtr0072865; FBpp0072744; FBgn0002183 [Q8IRG6-1] |
GeneIDi | 38248 |
KEGGi | dme:Dmel_CG1828 |
Organism-specific databases
CTDi | 38248 |
FlyBasei | FBgn0002183, dre4 |
VEuPathDBi | VectorBase:FBgn0002183 |
Phylogenomic databases
eggNOGi | KOG1189, Eukaryota |
GeneTreei | ENSGT00390000014495 |
HOGENOMi | CLU_004627_0_0_1 |
InParanoidi | Q8IRG6 |
OMAi | HQFFLDG |
PhylomeDBi | Q8IRG6 |
Enzyme and pathway databases
Reactomei | R-DME-112382, Formation of RNA Pol II elongation complex R-DME-674695, RNA Polymerase II Pre-transcription Events R-DME-6796648, TP53 Regulates Transcription of DNA Repair Genes R-DME-6804756, Regulation of TP53 Activity through Phosphorylation R-DME-75955, RNA Polymerase II Transcription Elongation |
SignaLinki | Q8IRG6 |
Miscellaneous databases
BioGRID-ORCSi | 38248, 0 hits in 1 CRISPR screen |
GenomeRNAii | 38248 |
PROi | PR:Q8IRG6 |
Gene expression databases
Bgeei | FBgn0002183, Expressed in imaginal disc and 36 other tissues |
ExpressionAtlasi | Q8IRG6, baseline and differential |
Genevisiblei | Q8IRG6, DM |
Family and domain databases
CDDi | cd01091, CDC68-like, 1 hit |
DisProti | DP00721 |
Gene3Di | 2.30.29.30, 1 hit 3.40.350.10, 1 hit 3.90.230.10, 1 hit |
IDEALi | IID50095 |
InterProi | View protein in InterPro IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR013719, DUF1747 IPR029148, FACT-Spt16_Nlobe IPR013953, FACT_Spt16 IPR000994, Pept_M24 IPR011993, PH-like_dom_sf IPR040258, Spt16 IPR033825, Spt16_M24 |
PANTHERi | PTHR13980, PTHR13980, 1 hit |
Pfami | View protein in Pfam PF14826, FACT-Spt16_Nlob, 1 hit PF00557, Peptidase_M24, 1 hit PF08512, Rtt106, 1 hit PF08644, SPT16, 1 hit |
SMARTi | View protein in SMART SM01285, FACT-Spt16_Nlob, 1 hit SM01287, Rtt106, 1 hit SM01286, SPT16, 1 hit |
SUPFAMi | SSF55920, SSF55920, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPT16_DROME | |
Accessioni | Q8IRG6Primary (citable) accession number: Q8IRG6 Secondary accession number(s): O17045 Q9W071 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families