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Protein

Slowpoke-binding protein

Gene

Slob

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of calcium-activated channel Slo. Increases or decreases the voltage sensitivity of Slo, depending on the absence or presence of 14-3-3-zeta in the complex, respectively.1 Publication

GO - Molecular functioni

  • protein kinase activity Source: FlyBase

GO - Biological processi

  • modulation of chemical synaptic transmission Source: UniProtKB
  • negative regulation of ATPase activity Source: GO_Central
  • negative regulation of ion transport Source: GO_Central
  • neuromuscular synaptic transmission Source: FlyBase
  • regulation of membrane potential Source: FlyBase
  • response to starvation Source: FlyBase

Names & Taxonomyi

Protein namesi
Recommended name:
Slowpoke-binding protein
Gene namesi
Name:Slob
ORF Names:CG43756
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0264087 Slob

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54S → A: Abolishes phosphorylation and interaction with 14-3-3-zeta; when associated with A-79. 1 Publication1
Mutagenesisi79S → A: Abolishes phosphorylation and interaction with 14-3-3-zeta; when associated with A-54. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000719591 – 515Slowpoke-binding proteinAdd BLAST515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Phosphoserine1 Publication1
Modified residuei79Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation of Ser-54 and Ser-79 is required for the interaction with 14-3-3-zeta but not with that of Slo.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8IPH9
PRIDEiQ8IPH9

PTM databases

iPTMnetiQ8IPH9

Expressioni

Tissue specificityi

Expressed in head. In larval brain, it is expressed in the mushroom body. Also expressed in larval muscles.1 Publication

Inductioni

Expressed with a circadian rhythm showing a peak at ZT15 (zeitgeber 15).1 Publication

Gene expression databases

BgeeiFBgn0264087 Expressed in 16 organ(s), highest expression level in head
ExpressionAtlasiQ8IPH9 baseline and differential
GenevisibleiQ8IPH9 DM

Interactioni

Subunit structurei

Interacts specifically with Slo; which activates Slo activity. Interacts with 14-3-3-zeta when phosphorylated. Forms a heterotetrameric complex containing phosphorylated Slob, Slo and 14-3-3-zeta, which represses Slo activity due to the indirect interaction between Slo and 14-3-3-zeta.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi60180, 4 interactors
ELMiQ8IPH9
IntActiQ8IPH9, 4 interactors
STRINGi7227.FBpp0303549

Structurei

3D structure databases

ProteinModelPortaliQ8IPH9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 203Interaction with SloAdd BLAST13

Phylogenomic databases

eggNOGiENOG410IKGX Eukaryota
ENOG410YSBT LUCA
GeneTreeiENSGT00390000017669
InParanoidiQ8IPH9
KOiK17543
OrthoDBiEOG091G0II9
PhylomeDBiQ8IPH9

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
SUPFAMiSSF56112 SSF56112, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8IPH9-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFKFNKAAQQ QRIDNRNSAV TGHDPFVRPP VPEKKVRNIM KKLHKANGLK
60 70 80 90 100
RSNSAIEFDV SALTAANRRQ IYSSNRSASS EQDNSDLSEH SEKSPLVSAR
110 120 130 140 150
LDNLARLFFS KSMPAETGSR DTIDSVLTTR PNIKYQYSAL DSGNGIVERS
160 170 180 190 200
PRERAQREKA LNATQEWIQG ANGRYEVIAH LDEIGSRHGK NWFLVTDASV
210 220 230 240 250
RTDRLQTLLP LPPDCVAFED LPPNECAREI LMELLGSLHH PYIYPVLDLG
260 270 280 290 300
FLRNSSYNYA CLVTPFNSRG SLKDLIYKAQ WNEPWARKYT RKPNGLPVSQ
310 320 330 340 350
VQRLGRQILE ALLFLKERGF PLHGHLHSGN VILQNGAARL SGLENGLLGL
360 370 380 390 400
SSRINAVMWS RSVTEIENVD IVCFGHLLYE MCTGQELTTP KPSMRVLEME
410 420 430 440 450
LQHYPQIGQT RKQILEILGL IFEPPSGVCP SVEDLVLCDL FRSIDLRELR
460 470 480 490 500
GPCFSTIKPS LSRSTLNLLQ AVKKRQCASL GHSLSEANSP CTPPSTPHDR
510
RTGVLPAPYE VFRMY
Length:515
Mass (Da):57,924
Last modified:April 26, 2004 - v2
Checksum:i1B4D86959CCAFAC1
GO
Isoform 2 (identifier: Q8IPH9-2) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     410-413: Missing.

Show »
Length:511
Mass (Da):57,411
Checksum:iFC9E6BE61A8B6A9B
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9VLZ5Q9VLZ5_DROME
Slowpoke binding protein, isoform K
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
1,191Annotation score:
A4V0D4A4V0D4_DROME
Slowpoke binding protein, isoform C
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
515Annotation score:
M9MRG6M9MRG6_DROME
Slowpoke binding protein, isoform G
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
459Annotation score:
M9MRE3M9MRE3_DROME
Slowpoke binding protein, isoform H
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
455Annotation score:
M9MRL0M9MRL0_DROME
Slowpoke binding protein, isoform F
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
578Annotation score:
M9PF34M9PF34_DROME
Slowpoke binding protein, isoform I
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
1,280Annotation score:
M9PB66M9PB66_DROME
Slowpoke binding protein, isoform J
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
1,347Annotation score:
M9MRD7M9MRD7_DROME
Slowpoke binding protein, isoform E
Slob CG13792, CG6772, Dmel\CG43756, slob, CG43756
511Annotation score:

Sequence cautioni

The sequence AAL28269 differs from that shown. Reason: Frameshift at position 262.Curated
The sequence AAN10635 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 13RI → PS in AAC06289 (PubMed:9539129).Curated2
Sequence conflicti47N → T in AAC06289 (PubMed:9539129).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010097410 – 413Missing in isoform 2. 3 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051162 mRNA Translation: AAC06289.1
AE014134 Genomic DNA Translation: AAF52534.1
AE014134 Genomic DNA Translation: AAN10635.1 Sequence problems.
AE014134 Genomic DNA Translation: ABC65880.1
BT021228 mRNA Translation: AAX33376.1
AY060721 mRNA Translation: AAL28269.1 Frameshift.
RefSeqiNP_001033881.1, NM_001038792.1 [Q8IPH9-2]
NP_001188710.1, NM_001201781.1 [Q8IPH9-2]
NP_477349.1, NM_058001.3 [Q8IPH9-1]
NP_723292.1, NM_164756.3
NP_723293.1, NM_164757.2 [Q8IPH9-1]
UniGeneiDm.5645

Genome annotation databases

EnsemblMetazoaiFBtr0330697; FBpp0303540; FBgn0264087 [Q8IPH9-1]
FBtr0330699; FBpp0303542; FBgn0264087 [Q8IPH9-1]
FBtr0330700; FBpp0303543; FBgn0264087 [Q8IPH9-2]
FBtr0330701; FBpp0303544; FBgn0264087 [Q8IPH9-2]
GeneIDi34038
KEGGidme:Dmel_CG43756
UCSCiCG6772-RD d. melanogaster

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051162 mRNA Translation: AAC06289.1
AE014134 Genomic DNA Translation: AAF52534.1
AE014134 Genomic DNA Translation: AAN10635.1 Sequence problems.
AE014134 Genomic DNA Translation: ABC65880.1
BT021228 mRNA Translation: AAX33376.1
AY060721 mRNA Translation: AAL28269.1 Frameshift.
RefSeqiNP_001033881.1, NM_001038792.1 [Q8IPH9-2]
NP_001188710.1, NM_001201781.1 [Q8IPH9-2]
NP_477349.1, NM_058001.3 [Q8IPH9-1]
NP_723292.1, NM_164756.3
NP_723293.1, NM_164757.2 [Q8IPH9-1]
UniGeneiDm.5645

3D structure databases

ProteinModelPortaliQ8IPH9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60180, 4 interactors
ELMiQ8IPH9
IntActiQ8IPH9, 4 interactors
STRINGi7227.FBpp0303549

PTM databases

iPTMnetiQ8IPH9

Proteomic databases

PaxDbiQ8IPH9
PRIDEiQ8IPH9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0330697; FBpp0303540; FBgn0264087 [Q8IPH9-1]
FBtr0330699; FBpp0303542; FBgn0264087 [Q8IPH9-1]
FBtr0330700; FBpp0303543; FBgn0264087 [Q8IPH9-2]
FBtr0330701; FBpp0303544; FBgn0264087 [Q8IPH9-2]
GeneIDi34038
KEGGidme:Dmel_CG43756
UCSCiCG6772-RD d. melanogaster

Organism-specific databases

CTDi34038
FlyBaseiFBgn0264087 Slob

Phylogenomic databases

eggNOGiENOG410IKGX Eukaryota
ENOG410YSBT LUCA
GeneTreeiENSGT00390000017669
InParanoidiQ8IPH9
KOiK17543
OrthoDBiEOG091G0II9
PhylomeDBiQ8IPH9

Miscellaneous databases

GenomeRNAii34038
PROiPR:Q8IPH9

Gene expression databases

BgeeiFBgn0264087 Expressed in 16 organ(s), highest expression level in head
ExpressionAtlasiQ8IPH9 baseline and differential
GenevisibleiQ8IPH9 DM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
SUPFAMiSSF56112 SSF56112, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSLOB_DROME
AccessioniPrimary (citable) accession number: Q8IPH9
Secondary accession number(s): O61564
, Q5BIJ6, Q95SL3, Q9VLZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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Main funding by: National Institutes of Health

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