Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RAC serine/threonine-protein kinase

Gene

Akt1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase involved in various developmental processes (PubMed:9601646, PubMed:10587646, PubMed:10962553, PubMed:11740943, PubMed:12172554, PubMed:11872800, PubMed:14525946, PubMed:12893776, PubMed:15466161, PubMed:15712201). During early embryogenesis, acts as a survival protein (PubMed:9601646, PubMed:10962553). During mid-embryogenesis, phosphorylates and activates trh, a transcription factor required for tracheal cell fate determination (PubMed:11740943). Also regulates tracheal cell migration (PubMed:11740943, PubMed:14525946). Later in development, acts downstream of PI3K and Pk61C/PDK1 in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and antagonizing FOXO transcription factor (PubMed:10587646, PubMed:10962553, PubMed:11752451, PubMed:12893776, PubMed:25329475, PubMed:29025897, PubMed:24603715). Controls follicle cell size during oogenesis (PubMed:15712201). May also stimulate cell growth by phosphorylating Gig/Tsc2 and inactivating the Tsc complex (PubMed:12172554, PubMed:15466161). Dephosphorylation of 'Ser-586' by Phlpp triggers apoptosis and suppression of tumor growth (PubMed:10962553).14 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei295ATPCurated1
Active sitei389Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 280ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphatidylinositol binding Source: FlyBase
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Growth regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-110478 Insulin signaling pathway
R-DME-110523 TOR signaling pathway
R-DME-1257604 PIP3 activates AKT signaling
R-DME-1358803 Downregulation of ERBB2:ERBB3 signaling
R-DME-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-DME-165158 Activation of AKT2
R-DME-165159 mTOR signalling
R-DME-165181 Inhibition of TSC complex formation by PKB
R-DME-198323 AKT phosphorylates targets in the cytosol
R-DME-198693 AKT phosphorylates targets in the nucleus
R-DME-199418 Negative regulation of the PI3K/AKT network
R-DME-203615 eNOS activation
R-DME-211163 AKT-mediated inactivation of FOXO1A
R-DME-389357 CD28 dependent PI3K/Akt signaling
R-DME-389513 CTLA4 inhibitory signaling
R-DME-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-DME-450604 KSRP (KHSRP) binds and destabilizes mRNA
R-DME-5218920 VEGFR2 mediated vascular permeability
R-DME-5628897 TP53 Regulates Metabolic Genes
R-DME-6804758 Regulation of TP53 Activity through Acetylation
R-DME-6804759 Regulation of TP53 Activity through Association with Co-factors
R-DME-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-DME-8876198 RAB GEFs exchange GTP for GDP on RABs
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9604323 Negative regulation of NOTCH4 signaling
SignaLinkiQ8INB9

Names & Taxonomyi

Protein namesi
Recommended name:
RAC serine/threonine-protein kinase (EC:2.7.11.1)
Short name:
DAkt
Short name:
DRAC-PK
Short name:
Dakt1
Alternative name(s):
Akt
Protein kinase B
Short name:
PKB
Gene namesi
Name:Akt1Imported
ORF Names:CG4006Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0010379 Akt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Death at the first instar larval stage (PubMed:9601646). Conditional RNAi-mediated knockdown in the female germline reduces ovary size (PubMed:24786828). RNAi-mediated knockdown in both larval salivary glands and fat body, results in small salivary glands displaying ectopic lipid storage and reduced expression of CdsA (PubMed:24603715).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi180G → S: Fails to be recruited at the membrane upon activation. 1 Publication1
Mutagenesisi260K → A: Abolishes enzymatic activity. 1
Mutagenesisi295K → M: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi408F → I: Abolishes enzymatic activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000457841 – 611RAC serine/threonine-protein kinaseAdd BLAST611

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30Phosphoserine1 Publication1
Modified residuei586Phosphoserine3 Publications1

Post-translational modificationi

Phosphorylated and activated by Pk61C/PDK1 (PubMed:11344272). Phosphorylated on Ser-586 by the TORC2 complex (PubMed:10962553, PubMed:15718470, PubMed:22493059).4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8INB9
PRIDEiQ8INB9

PTM databases

iPTMnetiQ8INB9

Expressioni

Tissue specificityi

Ubiquitously expressed. Present in ovary, where it is concentrated at the basal side of follicle cells.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Strongly expressed in embryo and pupa. Weakly expressed in larva. Mildly expressed in adult.2 Publications

Gene expression databases

BgeeiFBgn0010379 Expressed in 48 organ(s), highest expression level in embryo
ExpressionAtlasiQ8INB9 baseline and differential
GenevisibleiQ8INB9 DM

Interactioni

Subunit structurei

Interacts with trbl.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi67008, 197 interactors
DIPiDIP-49060N
IntActiQ8INB9, 43 interactors
MINTiQ8INB9
STRINGi7227.FBpp0082682

Structurei

3D structure databases

ProteinModelPortaliQ8INB9
SMRiQ8INB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 211PHPROSITE-ProRule annotationAdd BLAST106
Domaini266 – 523Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini524 – 597AGC-kinase C-terminalAdd BLAST74

Domaini

Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in its targeting to the plasma membrane.Curated

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0598 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00930000150811
InParanoidiQ8INB9
KOiK04456
OrthoDBiEOG091G06FF
PhylomeDBiQ8INB9

Family and domain databases

CDDicd01241 PH_PKB, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR039026 PH_PKB
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR039029 RAC_gamma-like
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24356:SF190 PTHR24356:SF190, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform C (identifier: Q8INB9-1) [UniParc]FASTAAdd to basket
Also known as: PK85

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNYLPFVLQR RSTVVASAPA PGSASRIPES PTTTGSNIIN IIYSQSTHPN
60 70 80 90 100
SSPTSGSAEK FSWQQSWPSR TSAAPTHDSG TMSINTTFDL SSPSVTSGHA
110 120 130 140 150
LTEQTQVVKE GWLMKRGEHI KNWRQRYFVL HSDGRLMGYR SKPADSASTP
160 170 180 190 200
SDFLLNNFTV RGCQIMTVDR PKPFTFIIRG LQWTTVIERT FAVESELERQ
210 220 230 240 250
QWTEAIRNVS SRLIDVGEVA MTPSEQTDMT DVDMATIAED ELSEQFSVQG
260 270 280 290 300
TTCNSSGVKK VTLENFEFLK VLGKGTFGKV ILCREKATAK LYAIKILKKE
310 320 330 340 350
VIIQKDEVAH TLTESRVLKS TNHPFLISLK YSFQTNDRLC FVMQYVNGGE
360 370 380 390 400
LFWHLSHERI FTEDRTRFYG AEIISALGYL HSQGIIYRDL KLENLLLDKD
410 420 430 440 450
GHIKVADFGL CKEDITYGRT TKTFCGTPEY LAPEVLDDND YGQAVDWWGT
460 470 480 490 500
GVVMYEMICG RLPFYNRDHD VLFTLILVEE VKFPRNITDE AKNLLAGLLA
510 520 530 540 550
KDPKKRLGGG KDDVKEIQAH PFFASINWTD LVLKKIPPPF KPQVTSDTDT
560 570 580 590 600
RYFDKEFTGE SVELTPPDPT GPLGSIAEEP LFPQFSYQGD MASTLGTSSH
610
ISTSTSLASM Q
Length:611
Mass (Da):68,485
Last modified:December 7, 2004 - v3
Checksum:iC139380152580934
GO
Isoform A (identifier: Q8INB9-2) [UniParc]FASTAAdd to basket
Also known as: PK66

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.

Note: Major form.
Show »
Length:530
Mass (Da):59,911
Checksum:iF7508582C7F4A288
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0B4LIA3A0A0B4LIA3_DROME
Non-specific serine/threonine prote...
Akt1 AKT, Akt, akt, AKT1, akt1
530Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73A → T in CAA58499 (PubMed:7876156).Curated1
Sequence conflicti200 – 201QQ → HE in CAA81204 (PubMed:8302573).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188331 – 81Missing in isoform A. 2 PublicationsAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26242 mRNA Translation: CAA81204.1
X83510 Genomic DNA Translation: CAA58499.2
X83510 Genomic DNA Translation: CAA58500.1
AE014297 Genomic DNA Translation: AAF55275.1
AE014297 Genomic DNA Translation: AAN13699.3
AY069856 mRNA Translation: AAL40001.1
PIRiA55888
RefSeqiNP_001287353.1, NM_001300424.1 [Q8INB9-2]
NP_001287354.1, NM_001300425.1 [Q8INB9-2]
NP_732113.3, NM_169705.2 [Q8INB9-1]
NP_732114.1, NM_169706.2 [Q8INB9-2]
NP_732115.1, NM_169707.2 [Q8INB9-2]
UniGeneiDm.1219

Genome annotation databases

EnsemblMetazoaiFBtr0083226; FBpp0082680; FBgn0010379 [Q8INB9-2]
FBtr0083227; FBpp0082681; FBgn0010379 [Q8INB9-2]
FBtr0083228; FBpp0082682; FBgn0010379 [Q8INB9-1]
FBtr0344470; FBpp0310841; FBgn0010379 [Q8INB9-2]
FBtr0344717; FBpp0311050; FBgn0010379 [Q8INB9-2]
GeneIDi41957
KEGGidme:Dmel_CG4006

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26242 mRNA Translation: CAA81204.1
X83510 Genomic DNA Translation: CAA58499.2
X83510 Genomic DNA Translation: CAA58500.1
AE014297 Genomic DNA Translation: AAF55275.1
AE014297 Genomic DNA Translation: AAN13699.3
AY069856 mRNA Translation: AAL40001.1
PIRiA55888
RefSeqiNP_001287353.1, NM_001300424.1 [Q8INB9-2]
NP_001287354.1, NM_001300425.1 [Q8INB9-2]
NP_732113.3, NM_169705.2 [Q8INB9-1]
NP_732114.1, NM_169706.2 [Q8INB9-2]
NP_732115.1, NM_169707.2 [Q8INB9-2]
UniGeneiDm.1219

3D structure databases

ProteinModelPortaliQ8INB9
SMRiQ8INB9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67008, 197 interactors
DIPiDIP-49060N
IntActiQ8INB9, 43 interactors
MINTiQ8INB9
STRINGi7227.FBpp0082682

PTM databases

iPTMnetiQ8INB9

Proteomic databases

PaxDbiQ8INB9
PRIDEiQ8INB9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083226; FBpp0082680; FBgn0010379 [Q8INB9-2]
FBtr0083227; FBpp0082681; FBgn0010379 [Q8INB9-2]
FBtr0083228; FBpp0082682; FBgn0010379 [Q8INB9-1]
FBtr0344470; FBpp0310841; FBgn0010379 [Q8INB9-2]
FBtr0344717; FBpp0311050; FBgn0010379 [Q8INB9-2]
GeneIDi41957
KEGGidme:Dmel_CG4006

Organism-specific databases

CTDi207
FlyBaseiFBgn0010379 Akt1

Phylogenomic databases

eggNOGiKOG0598 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00930000150811
InParanoidiQ8INB9
KOiK04456
OrthoDBiEOG091G06FF
PhylomeDBiQ8INB9

Enzyme and pathway databases

ReactomeiR-DME-110478 Insulin signaling pathway
R-DME-110523 TOR signaling pathway
R-DME-1257604 PIP3 activates AKT signaling
R-DME-1358803 Downregulation of ERBB2:ERBB3 signaling
R-DME-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-DME-165158 Activation of AKT2
R-DME-165159 mTOR signalling
R-DME-165181 Inhibition of TSC complex formation by PKB
R-DME-198323 AKT phosphorylates targets in the cytosol
R-DME-198693 AKT phosphorylates targets in the nucleus
R-DME-199418 Negative regulation of the PI3K/AKT network
R-DME-203615 eNOS activation
R-DME-211163 AKT-mediated inactivation of FOXO1A
R-DME-389357 CD28 dependent PI3K/Akt signaling
R-DME-389513 CTLA4 inhibitory signaling
R-DME-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-DME-450604 KSRP (KHSRP) binds and destabilizes mRNA
R-DME-5218920 VEGFR2 mediated vascular permeability
R-DME-5628897 TP53 Regulates Metabolic Genes
R-DME-6804758 Regulation of TP53 Activity through Acetylation
R-DME-6804759 Regulation of TP53 Activity through Association with Co-factors
R-DME-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-DME-8876198 RAB GEFs exchange GTP for GDP on RABs
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9604323 Negative regulation of NOTCH4 signaling
SignaLinkiQ8INB9

Miscellaneous databases

ChiTaRSiAkt1 fly
GenomeRNAii41957
PROiPR:Q8INB9

Gene expression databases

BgeeiFBgn0010379 Expressed in 48 organ(s), highest expression level in embryo
ExpressionAtlasiQ8INB9 baseline and differential
GenevisibleiQ8INB9 DM

Family and domain databases

CDDicd01241 PH_PKB, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR039026 PH_PKB
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR039029 RAC_gamma-like
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24356:SF190 PTHR24356:SF190, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAKT1_DROME
AccessioniPrimary (citable) accession number: Q8INB9
Secondary accession number(s): Q0KI65
, Q24293, Q24469, Q24470, Q7JN11, Q8T9A5, Q9VEY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: December 7, 2004
Last modified: November 7, 2018
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again