UniProtKB - Q8IAS0 (PLM10_PLAF7)
Plasmepsin X
PMX
Functioni
During the asexual blood stage, processes key proteins essential for merozoite egress and invasion of host erythrocytes (PubMed:29074775, PubMed:32109369).
Cleaves and activates proteases SUB1 and SUB2 (PubMed:29074775, PubMed:32109369).
May process members of the EBL and Rh protein families (PubMed:32109369).
Also cleaves apical membrane protein AMA1 (PubMed:29074775).
During the mosquito vector stage and probably in ookinetes, cleaves CelTOS (PubMed:29074775).
2 PublicationsActivity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 266 | PROSITE-ProRule annotation | 1 | |
Active sitei | 457 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB
GO - Biological processi
- entry into host cell by a symbiont-containing vacuole Source: UniProtKB
- protein autoprocessing Source: UniProtKB
- protein processing Source: UniProtKB
- proteolysis Source: GeneDB
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Protein family/group databases
MEROPSi | A01.A93 |
Names & Taxonomyi
Protein namesi | Recommended name: Plasmepsin X1 Publication (EC:3.4.23.-2 Publications)Short name: PfPMX1 Publication Alternative name(s): Plasmepsin 10Curated |
Gene namesi | Name:PMX1 Publication ORF Names:PF3D7_0808200Imported |
Organismi | Plasmodium falciparum (isolate 3D7)Imported |
Taxonomic identifieri | 36329 [NCBI] |
Taxonomic lineagei | Eukaryota › Sar › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodiidae › Plasmodium › Plasmodium (Laverania) › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | PlasmoDB:PF3D7_0808200 |
Subcellular locationi
Other locations
- secretory vesicle By similarity
Note: In schizonts, localizes to exonemes which are secretory vesicles that discharge their content during egress into the parasitophorous vacuole.By similarity
Other locations
- transport vesicle Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasmic vesiclePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 266 | D → A: Loss of catalytic activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4523390 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
PropeptideiPRO_0000453739 | 27 – ? | Curated | ||
ChainiPRO_0000453740 | ? – 573 | Plasmepsin XSequence analysis |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 39 ↔ 51 | Combined sources1 Publication | ||
Disulfide bondi | 42 ↔ 48 | Combined sources1 Publication | ||
Disulfide bondi | 279 ↔ 284 | Combined sources1 Publication | ||
Glycosylationi | 334 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 447 ↔ 448 | Combined sources1 Publication | ||
Disulfide bondi | 482 ↔ 521 | Combined sources1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PRIDEi | Q8IAS0 |
Interactioni
Protein-protein interaction databases
IntActi | Q8IAS0, 2 interactors |
STRINGi | 5833.PF08_0108 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 248 – 567 | Peptidase A1PROSITE-ProRule annotationAdd BLAST | 320 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 167 – 211 | DisorderedSequence analysisAdd BLAST | 45 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
HOGENOMi | CLU_500120_0_0_1 |
InParanoidi | Q8IAS0 |
OMAi | PQEIHPI |
PhylomeDBi | Q8IAS0 |
Family and domain databases
CDDi | cd05471, pepsin_like, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR034164, Pepsin-like_dom IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 1 hit PS51767, PEPTIDASE_A1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MKRISPLNTL FYLSLFFSYT FKGLKCTRIY KIGTKALPCS ECHDVFDCTG
60 70 80 90 100
CLFEEKESSH VIPLKLNKKN PNDHKKLQKH HESLKLGDVK YYVNRGEGIS
110 120 130 140 150
GSLGTSSGNT LDDMDLINEE INKKRTNAQL DEKNFLDFTT YNKNKAQDIS
160 170 180 190 200
DHLSDIQKHV YEQDAQKGNK NFTNNENNSD NENNSDNENN SDNENNLDNE
210 220 230 240 250
NNLDNENNSD NSSIEKNFIA LENKNATVEQ TKENIFLVPL KHLRDSQFVG
260 270 280 290 300
ELLVGTPPQT VYPIFDTGST NVWVVTTACE EESCKKVRRY DPNKSKTFRR
310 320 330 340 350
SFIEKNLHIV FGSGSISGSV GTDTFMLGKH LVRNQTFGLV ESESNNNKNG
360 370 380 390 400
GDNIFDYISF EGIVGLGFPG MLSAGNIPFF DNLLKQNPNV DPQFSFYISP
410 420 430 440 450
YDGKSTLIIG GISKSFYEGD IYMLPVLKES YWEVKLDELY IGKERICCDE
460 470 480 490 500
ESYVIFDTGT SYNTMPSSQM KTFLNLIHST ACTEQNYKDI LKSYPIIKYV
510 520 530 540 550
FGELIIELHP EEYMILNDDV CMPAYMQIDV PSERNHAYLL GSLSFMRNFF
560 570
TVFVRGTESR PSMVGVARAK SKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL844507 Genomic DNA Translation: CAD51290.1 |
RefSeqi | XP_001349441.1, XM_001349405.1 |
Genome annotation databases
EnsemblProtistsi | CAD51290; CAD51290; PF3D7_0808200 |
GeneIDi | 2655308 |
KEGGi | pfa:PF3D7_0808200 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL844507 Genomic DNA Translation: CAD51290.1 |
RefSeqi | XP_001349441.1, XM_001349405.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6ORS | X-ray | 2.30 | A | 28-573 | [»] | |
SMRi | Q8IAS0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q8IAS0, 2 interactors |
STRINGi | 5833.PF08_0108 |
Chemistry databases
ChEMBLi | CHEMBL4523390 |
Protein family/group databases
MEROPSi | A01.A93 |
Proteomic databases
PRIDEi | Q8IAS0 |
Genome annotation databases
EnsemblProtistsi | CAD51290; CAD51290; PF3D7_0808200 |
GeneIDi | 2655308 |
KEGGi | pfa:PF3D7_0808200 |
Organism-specific databases
VEuPathDBi | PlasmoDB:PF3D7_0808200 |
Phylogenomic databases
HOGENOMi | CLU_500120_0_0_1 |
InParanoidi | Q8IAS0 |
OMAi | PQEIHPI |
PhylomeDBi | Q8IAS0 |
Family and domain databases
CDDi | cd05471, pepsin_like, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR034164, Pepsin-like_dom IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 1 hit PS51767, PEPTIDASE_A1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLM10_PLAF7 | |
Accessioni | Q8IAS0Primary (citable) accession number: Q8IAS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 29, 2021 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families