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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

PF3D7_0417200

Organism
Plasmodium falciparum (isolate 3D7)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism.UniRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase (PF3D7_0417200)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei490UniRule annotation1

GO - Molecular functioni

  • dihydrofolate reductase activity Source: InterPro
  • RNA binding Source: GeneDB
  • thymidylate synthase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationImported, OxidoreductaseUniRule annotationImported, Transferase
Biological processNucleotide biosynthesisUniRule annotation, One-carbon metabolismUniRule annotation

Enzyme and pathway databases

ReactomeiR-PFA-499943 Interconversion of nucleotide di- and triphosphates
SABIO-RKiQ8I1R6
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
Gene namesi
ORF Names:PF3D7_0417200Imported
OrganismiPlasmodium falciparum (isolate 3D7)Imported
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0417200

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GeneDB

PTM / Processingi

Proteomic databases

PRIDEiQ8I1R6

Structurei

3D structure databases

ProteinModelPortaliQ8I1R6
SMRiQ8I1R6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 228DHFRInterPro annotationAdd BLAST219

Sequence similaritiesi

In the C-terminal section; belongs to the thymidylate synthase family.UniRule annotation
In the N-terminal section; belongs to the dihydrofolate reductase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257901
InParanoidiQ8I1R6
KOiK13998
OMAiICACCKV
PhylomeDBiQ8I1R6

Family and domain databases

CDDicd00209 DHFR, 1 hit
cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
3.40.430.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR012262 DHFR-TS
IPR017925 DHFR_CS
IPR001796 DHFR_dom
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PF00303 Thymidylat_synt, 1 hit
PIRSFiPIRSF000389 DHFR-TS, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF53597 SSF53597, 1 hit
SSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8I1R6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEQVCDVFD IYAICACCKV ESKNEGKKNE VFNNYTFRGL GNKGVLPWKC
60 70 80 90 100
NSLDMKYFCA VTTYVNESKY EKLKYKRCKY LNKETVDNVN DMPNSKKLQN
110 120 130 140 150
VVVMGRTSWE SIPKKFKPLS NRINVILSRT LKKEDFDEDV YIINKVEDLI
160 170 180 190 200
VLLGKLNYYK CFIIGGSVVY QEFLEKKLIK KIYFTRINST YECDVFFPEI
210 220 230 240 250
NENEYQIISV SDVYTSNNTT LDFIIYKKTN NKMLNEQNCI KGEEKNNDMP
260 270 280 290 300
LKNDDKDTCH MKKLTEFYKN VDKYKINYEN DDDDEEEDDF VYFNFNKEKE
310 320 330 340 350
EKNKNSIHPN DFQIYNSLKY KYHPEYQYLN IIYDIMMNGN KQSDRTGVGV
360 370 380 390 400
LSKFGYIMKF DLSQYFPLLT TKKLFLRGII EELLWFIRGE TNGNTLLNKN
410 420 430 440 450
VRIWEANGTR EFLDNRKLFH REVNDLGPIY GFQWRHFGAE YTNMYDNYEN
460 470 480 490 500
KGVDQLKNII NLIKNDPTSR RILLCAWNVK DLDQMALPPC HILCQFYVFD
510 520 530 540 550
GKLSCIMYQR SCDLGLGVPF NIASYSIFTH MIAQVCNLQP AQFIHVLGNA
560 570 580 590 600
HVYNNHIDSL KIQLNRIPYP FPTLKLNPDI KNIEDFTISD FTIQNYVHHE

KISMDMAA
Length:608
Mass (Da):71,737
Last modified:March 1, 2003 - v1
Checksum:i26FD4BB4A5F1F4A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844503 Genomic DNA Translation: CAD49208.1
RefSeqiXP_001351479.1, XM_001351443.1

Genome annotation databases

EnsemblProtistsiCAD49208; CAD49208; PF3D7_0417200
GeneDBiPF3D7_0417200.1:pep
GeneIDi9221804
KEGGipfa:PFD0830w

Similar proteinsi

Entry informationi

Entry nameiQ8I1R6_PLAF7
AccessioniPrimary (citable) accession number: Q8I1R6
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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