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Protein

Polypeptide N-acetylgalactosaminyltransferase 4

Gene

gly-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191SubstrateBy similarity1
Binding sitei216SubstrateBy similarity1
Metal bindingi239ManganeseBy similarity1
Binding sitei240SubstrateBy similarity1
Metal bindingi241ManganeseBy similarity1
Binding sitei346SubstrateBy similarity1
Metal bindingi374ManganeseBy similarity1
Binding sitei377SubstrateBy similarity1
Binding sitei380SubstrateBy similarity1
Binding sitei382SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-CEL-913709 O-linked glycosylation of mucins
UniPathwayi
UPA00378

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 4 (EC:2.4.1.41)
Short name:
pp-GaNTase 4
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
Gene namesi
Name:gly-4
ORF Names:Y116F11B.12
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY116F11B.12a ; CE26046 ; WBGene00001629 ; gly-4
Y116F11B.12b ; CE32074 ; WBGene00001629 ; gly-4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini32 – 589LumenalSequence analysisAdd BLAST558

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591471 – 589Polypeptide N-acetylgalactosaminyltransferase 4Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 369PROSITE-ProRule annotation
Disulfide bondi360 ↔ 438PROSITE-ProRule annotation
Disulfide bondi471 ↔ 488PROSITE-ProRule annotation
Disulfide bondi514 ↔ 531PROSITE-ProRule annotation
Glycosylationi523N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi553 ↔ 571PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8I136
PaxDbiQ8I136
PeptideAtlasiQ8I136
PRIDEiQ8I136

Expressioni

Gene expression databases

BgeeiWBGene00001629 Expressed in 5 organ(s), highest expression level in pharyngeal muscle cell (C elegans)

Interactioni

Protein-protein interaction databases

BioGridi45263, 2 interactors
DIPiDIP-26941N
IntActiQ8I136, 1 interactor
STRINGi6239.Y116F11B.12a.1

Structurei

3D structure databases

ProteinModelPortaliQ8I136
SMRiQ8I136
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini458 – 589Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 255Catalytic subdomain AAdd BLAST106
Regioni315 – 377Catalytic subdomain BAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi37 – 72Pro-richAdd BLAST36

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3738 Eukaryota
ENOG410XPRX LUCA
GeneTreeiENSGT00760000118828
HOGENOMiHOG000038227
InParanoidiQ8I136
KOiK00710
OMAiEDKYKAN
OrthoDBiEOG091G085O
PhylomeDBiQ8I136

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform a (identifier: Q8I136-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLPRMLKMKT VGTVLAVIWL FGLAFIYVQS TSSSLRPPGR HPPPLPQLDP
60 70 80 90 100
LIPQNPPQND EIRPKKSAPP IPTINLAEDT TIHERTEKDV TWKTFDVEKF
110 120 130 140 150
LNKGKWHQGE DKYKANSFNQ EASDALNPTR KIPDSREPQC RDVDYSKVGM
160 170 180 190 200
QPTTVIITYH NEARSSLLRT VFSVFNQSPE ELLLEIVLVD DNSQDVEIGK
210 220 230 240 250
ELAQIQRITV LRNNQREGLI RSRVKGAQVA RAPVLTFLDS HIECNQKWLE
260 270 280 290 300
PLLARIAENP KAVVAPIIDV INVDNFNYVG ASADLRGGFD WTLVFRWEFM
310 320 330 340 350
NEQLRKERHA HPTAPIRSPT MAGGLFAISK EWFNELGTYD LDMEVWGGEN
360 370 380 390 400
LEMSFRVWQC GGSLEIMPCS RVGHVFRKKH PYTFPGGSGN VFQKNTRRAA
410 420 430 440 450
EVWMDEYKAI YLKNVPSARF VNFGDITDRL AIRDRLQCKS FKWYLENVYP
460 470 480 490 500
QLEIPRKTPG KSFQMKIGNL CLDSMARKES EAPGLFGCHG TGGNQEWVFD
510 520 530 540 550
QLTKTFKNAI SQLCLDFSSN TENKTVTMVK CENLRPDTMV VEKNGWLTQG
560 570 580
GKCLTVNQGS GGDWLIYGAH CELNNGAQRW IFEKLDTYE
Length:589
Mass (Da):67,031
Last modified:August 16, 2004 - v2
Checksum:i108D621D91A329D2
GO
Isoform b (identifier: Q8I136-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     453-453: E → D
     454-589: Missing.

Note: No experimental confirmation available.
Show »
Length:453
Mass (Da):51,879
Checksum:i572763F5D4D25B53
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C6S3M5C6S3M5_CAEEL
Polypeptide N-acetylgalactosaminylt...
gly-4 CELE_Y116F11B.12, Y116F11B.12
480Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011236453E → D in isoform b. Curated1
Alternative sequenceiVSP_011237454 – 589Missing in isoform b. CuratedAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031834 mRNA Translation: AAC13670.1
AL132943 Genomic DNA Translation: CAB81985.3
AL132943 Genomic DNA Translation: CAC14394.1
PIRiT42244
RefSeqiNP_001024216.1, NM_001029045.3 [Q8I136-1]
NP_507850.2, NM_075449.7 [Q8I136-2]
UniGeneiCel.19666

Genome annotation databases

EnsemblMetazoaiY116F11B.12a.1; Y116F11B.12a.1; WBGene00001629 [Q8I136-1]
Y116F11B.12a.2; Y116F11B.12a.2; WBGene00001629 [Q8I136-1]
Y116F11B.12b.1; Y116F11B.12b.1; WBGene00001629 [Q8I136-2]
Y116F11B.12b.2; Y116F11B.12b.2; WBGene00001629 [Q8I136-2]
Y116F11B.12b.3; Y116F11B.12b.3; WBGene00001629 [Q8I136-2]
GeneIDi180302
KEGGicel:CELE_Y116F11B.12
UCSCiY116F11B.12b.1 c. elegans [Q8I136-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031834 mRNA Translation: AAC13670.1
AL132943 Genomic DNA Translation: CAB81985.3
AL132943 Genomic DNA Translation: CAC14394.1
PIRiT42244
RefSeqiNP_001024216.1, NM_001029045.3 [Q8I136-1]
NP_507850.2, NM_075449.7 [Q8I136-2]
UniGeneiCel.19666

3D structure databases

ProteinModelPortaliQ8I136
SMRiQ8I136
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45263, 2 interactors
DIPiDIP-26941N
IntActiQ8I136, 1 interactor
STRINGi6239.Y116F11B.12a.1

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Proteomic databases

EPDiQ8I136
PaxDbiQ8I136
PeptideAtlasiQ8I136
PRIDEiQ8I136

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY116F11B.12a.1; Y116F11B.12a.1; WBGene00001629 [Q8I136-1]
Y116F11B.12a.2; Y116F11B.12a.2; WBGene00001629 [Q8I136-1]
Y116F11B.12b.1; Y116F11B.12b.1; WBGene00001629 [Q8I136-2]
Y116F11B.12b.2; Y116F11B.12b.2; WBGene00001629 [Q8I136-2]
Y116F11B.12b.3; Y116F11B.12b.3; WBGene00001629 [Q8I136-2]
GeneIDi180302
KEGGicel:CELE_Y116F11B.12
UCSCiY116F11B.12b.1 c. elegans [Q8I136-1]

Organism-specific databases

CTDi180302
WormBaseiY116F11B.12a ; CE26046 ; WBGene00001629 ; gly-4
Y116F11B.12b ; CE32074 ; WBGene00001629 ; gly-4

Phylogenomic databases

eggNOGiKOG3738 Eukaryota
ENOG410XPRX LUCA
GeneTreeiENSGT00760000118828
HOGENOMiHOG000038227
InParanoidiQ8I136
KOiK00710
OMAiEDKYKAN
OrthoDBiEOG091G085O
PhylomeDBiQ8I136

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-CEL-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-CEL-913709 O-linked glycosylation of mucins

Miscellaneous databases

PROiPR:Q8I136

Gene expression databases

BgeeiWBGene00001629 Expressed in 5 organ(s), highest expression level in pharyngeal muscle cell (C elegans)

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGALT4_CAEEL
AccessioniPrimary (citable) accession number: Q8I136
Secondary accession number(s): O61390
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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