UniProtKB - Q8I0P1 (SPAST_DROME)
Spastin
spas
Functioni
ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle (PubMed:15242610, PubMed:15562320, PubMed:15823537, PubMed:16276413, PubMed:17452528, PubMed:25875445, PubMed:18202664, PubMed:19341724).
Regulates microtubule stability in the neuromuscular junction synapse (PubMed:15242610, PubMed:15562320, PubMed:19341724).
Involved in lipid metabolism by regulating the size and distribution of lipid droplets (PubMed:25875445).
Involved in axon regeneration by regulating microtubule severing (PubMed:23122959).
UniRule annotation9 PublicationsCatalytic activityi
- n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.UniRule annotation2 Publications EC:5.6.1.1
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 523 – 530 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
- ATP binding Source: UniProtKB-UniRule
- ATP hydrolysis activity Source: InterPro
- isomerase activity Source: UniProtKB-KW
- microtubule binding Source: FlyBase
- microtubule-severing ATPase activity Source: FlyBase
GO - Biological processi
- adult locomotory behavior Source: UniProtKB-UniRule
- cell division Source: UniProtKB-KW
- hemocyte migration Source: FlyBase
- locomotory behavior Source: FlyBase
- microtubule cytoskeleton organization Source: FlyBase
- microtubule severing Source: FlyBase
- mitotic chromosome movement towards spindle pole Source: UniProtKB-UniRule
- mitotic sister chromatid segregation Source: FlyBase
- mitotic spindle elongation Source: UniProtKB-UniRule
- negative regulation of microtubule depolymerization Source: FlyBase
- negative regulation of neuromuscular synaptic transmission Source: FlyBase
- negative regulation of synaptic assembly at neuromuscular junction Source: FlyBase
- positive regulation of axon extension involved in regeneration Source: FlyBase
- positive regulation of dendrite morphogenesis Source: FlyBase
- positive regulation of lipid metabolic process Source: FlyBase
- positive regulation of microtubule depolymerization Source: UniProtKB
- positive regulation of neuromuscular synaptic transmission Source: FlyBase
- positive regulation of synaptic assembly at neuromuscular junction Source: FlyBase
- protein hexamerization Source: UniProtKB-UniRule
- regulation of terminal button organization Source: FlyBase
Keywordsi
Molecular function | Developmental protein, Isomerase |
Biological process | Cell cycle, Cell division, Differentiation, Mitosis, Neurogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 5.6.1.1, 1994 |
Reactomei | R-DME-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III |
Names & Taxonomyi
Protein namesi | Recommended name: SpastinUniRule annotation (EC:5.6.1.1UniRule annotation2 Publications)Alternative name(s): D-Spastin Dm-Spastin Dspastin1 Publication |
Gene namesi | Name:spasUniRule annotation ORF Names:CG5977 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0039141, spas |
VEuPathDBi | VectorBase:FBgn0039141 |
Subcellular locationi
Cytoskeleton
- centrosome UniRule annotation1 Publication
- cytoskeleton UniRule annotation4 Publications
Other locations
- Membrane UniRule annotation; Peripheral membrane protein UniRule annotation
- Chromosome UniRule annotation1 Publication
- Lipid droplet UniRule annotation1 Publication
Note: Forms a intramembrane hairpin-like structure in the membrane (By similarity). Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules (PubMed:17452528). Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles (PubMed:15823537).UniRule annotation2 Publications
Cytoskeleton
- centrosome Source: UniProtKB
- microtubule Source: UniProtKB-UniRule
- microtubule cytoskeleton Source: UniProtKB
- spindle Source: UniProtKB-UniRule
Other locations
- chromosome Source: FlyBase
- cytoplasm Source: FlyBase
- integral component of membrane Source: UniProtKB-UniRule
- lipid droplet Source: UniProtKB-SubCell
- neuromuscular junction Source: FlyBase
- terminal bouton Source: FlyBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 121 | CytoplasmicUniRule annotationAdd BLAST | 121 | |
Intramembranei | 122 – 142 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 143 – 758 | CytoplasmicUniRule annotationAdd BLAST | 616 |
Keywords - Cellular componenti
Chromosome, Cytoplasm, Cytoskeleton, Lipid droplet, Membrane, MicrotubulePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 465 | L → A: Strongly impairs microtubule severing and weakly impairs ATPase activity; when associated with A-471 and A-472. 1 Publication | 1 | |
Mutagenesisi | 465 | L → F: Strongly impairs microtubule severing and weakly impairs ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 469 | I → A: Strongly impairs microtubule severing and ATPase activity but does not affect interaction with microtubules; when associated with A-473 and A-474. 1 Publication | 1 | |
Mutagenesisi | 471 | D → A: Strongly impairs microtubule severing and weakly impairs ATPase activity; when associated with A-465 and A-472. 1 Publication | 1 | |
Mutagenesisi | 472 | E → A: Strongly impairs microtubule severing and weakly impairs ATPase activity; when associated with A-465 and A-471. 1 Publication | 1 | |
Mutagenesisi | 473 | I → A: Strongly impairs microtubule severing and ATPase activity but does not affect interaction with microtubules; when associated with A-469 and A-474. 1 Publication | 1 | |
Mutagenesisi | 474 | V → A: Strongly impairs microtubule severing and ATPase activity but does not affect interaction with microtubules; when associated with A-469 and A-473. 1 Publication | 1 | |
Mutagenesisi | 503 | S → C: Impairs microtubule severing and ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 511 | G → R: Abrogates microtubule severing and strongly impairs ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 529 | K → R: Abrogates microtubule severing and ATPase activity. Induces accumulation of hyperstable microtubules at the neuromuscular junction presynpatic terminal and reduces synaptic area. Reduces adult lifespan and impairs climbing activity. 3 Publications | 1 | |
Mutagenesisi | 555 | K → A: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 556 | Y → A: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 557 | V → A: Abrogates microtubule severing and impairs ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 558 | G → A or V: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 559 | D → A or R: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 560 | G → A: Impairs microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 560 | G → V: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 561 | E → A or R: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 562 | K → A or R: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 583 | E → A: Abrogates microtubule severing and ATPase activity. 2 Publications | 1 | |
Mutagenesisi | 583 | E → Q: Impairs interaction with microtubules and promotes hexamerization. 2 Publications | 1 | |
Mutagenesisi | 589 | S → Y: Impairs microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 591 | R → A or E: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 595 | E → A: Impairs microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 595 | E → R: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 596 | H → A: Impairs microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 596 | H → D: Abrogates microtubule severing and impairs ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 596 | H → Y: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 597 | E → K: Impairs microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 598 | A → L: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 601 | R → E: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 601 | R → L: Abrogates microtubule severing and strongly impairs ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 631 | P → L: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 632 | Q → A: Strongly impairs microtubule severing and weakly impairs ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 633 | E → A: Weakly impairs microtubule severing and ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 633 | E → R: Impairs microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 697 | D → N: Weakly impairs microtubule severing and strongly impairs ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 704 | R → Q: Abrogates microtubule severing. 1 Publication | 1 | |
Mutagenesisi | 753 | Y → A: Strongly impairs microtubule severing and ATPase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367144 | 1 – 758 | SpastinAdd BLAST | 758 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 439 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8I0P1 |
PRIDEi | Q8I0P1 |
PTM databases
iPTMneti | Q8I0P1 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0039141, Expressed in female gonad and 54 other tissues |
ExpressionAtlasi | Q8I0P1, baseline and differential |
Genevisiblei | Q8I0P1, DM |
Interactioni
Subunit structurei
Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed (PubMed:18202664).
Interacts with microtubules (PubMed:15823537, PubMed:18202664).
Interacts with atl; may be involved in microtubule dynamics (PubMed:19341724).
UniRule annotation3 PublicationsGO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
- microtubule binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 67781, 6 interactors |
DIPi | DIP-59834N |
IntActi | Q8I0P1, 2 interactors |
STRINGi | 7227.FBpp0083918 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8I0P1 |
SMRi | Q8I0P1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8I0P1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 233 – 308 | MITSequence analysisAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 210 | Required for localization to punctate cytoplasmic fociAdd BLAST | 210 | |
Regioni | 1 – 159 | Interaction with atl1 PublicationAdd BLAST | 159 | |
Regioni | 1 – 103 | DisorderedSequence analysisAdd BLAST | 103 | |
Regioni | 169 – 202 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 208 – 758 | Sufficient for interaction with microtubules and microtubule severing2 PublicationsAdd BLAST | 551 | |
Regioni | 353 – 454 | DisorderedSequence analysisAdd BLAST | 102 | |
Regioni | 443 – 455 | Required for interaction with microtubules1 PublicationAdd BLAST | 13 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 53 | Polar residuesSequence analysisAdd BLAST | 53 | |
Compositional biasi | 60 – 103 | Polar residuesSequence analysisAdd BLAST | 44 | |
Compositional biasi | 169 – 201 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 359 – 375 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 386 – 411 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 421 – 454 | Polar residuesSequence analysisAdd BLAST | 34 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0740, Eukaryota |
GeneTreei | ENSGT00940000156258 |
InParanoidi | Q8I0P1 |
OMAi | GMTNEPM |
PhylomeDBi | Q8I0P1 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
HAMAPi | MF_03021, Spastin, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR007330, MIT_dom IPR027417, P-loop_NTPase IPR017179, Spastin |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00745, MIT, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MVRTKNQSSS SSASSSSTKS PIKSSSGAGS SGGGLGGRQS THRSSSASNV
60 70 80 90 100
AAVVAGGSSA AGGGSSSNRR SPGSSPDGDD DTTTTDDLTP TTCSPRSGHH
110 120 130 140 150
HSYGGYSSSV HKQNLYVVSF PIIFLFNVLR SLIYQLFCIF RYLYGASTKV
160 170 180 190 200
IYRPHRRDCN IEIVVQNSSK EQQQSLNHPS ELNREGDGQE QQLSNQPQRF
210 220 230 240 250
RPIQPLEMAA NRPGGGYSPG PGDPLLAKQK HHHRRAFEYI SKALKIDEEN
260 270 280 290 300
EGHKELAIEL YRKGIKELED GIAVDCWSGR GDVWDRAQRL HDKMQTNLSM
310 320 330 340 350
ARDRLHFLAL REQDLQMQRL SLKEKQKEEA QSKPQKTREP MLAGMTNEPM
360 370 380 390 400
KLRVRSSGYG PKATTSAQPT ASGRKLTIGS KRPVNLAVAN KSQTLPRNLG
410 420 430 440 450
SKTSVGAVQR QPAKTAATPP AVRRQFSSGR NTPPQRSRTP INNNGPSGSG
460 470 480 490 500
ASTPVVSVKG VEQKLVQLIL DEIVEGGAKV EWTDIAGQDV AKQALQEMVI
510 520 530 540 550
LPSVRPELFT GLRAPAKGLL LFGPPGNGKT LLARAVATEC SATFLNISAA
560 570 580 590 600
SLTSKYVGDG EKLVRALFAV ARHMQPSIIF IDEVDSLLSE RSSSEHEASR
610 620 630 640 650
RLKTEFLVEF DGLPGNPDGD RIVVLAATNR PQELDEAALR RFTKRVYVSL
660 670 680 690 700
PDEQTRELLL NRLLQKQGSP LDTEALRRLA KITDGYSGSD LTALAKDAAL
710 720 730 740 750
EPIRELNVEQ VKCLDISAMR AITEQDFHSS LKRIRRSVAP QSLNSYEKWS
QDYGDITI
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A126GV13 | A0A126GV13_DROME | Spastin | spas D-spastin, Dmel\CG5977, Dspastin, Spas, Spastin | 489 | Annotation score: | ||
A0A0B4LHJ5 | A0A0B4LHJ5_DROME | Spastin | spas D-spastin, Dmel\CG5977, Dspastin, Spas, Spastin | 696 | Annotation score: | ||
A0A126GV10 | A0A126GV10_DROME | Spastin | spas D-spastin, Dmel\CG5977, Dspastin, Spas, Spastin | 551 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 665 | Q → E in AAN71010 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 665 | Q → E in AAN71106 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF56223.3 AE014297 Genomic DNA Translation: AAN13975.2 AY069522 mRNA Translation: AAL39667.1 Different initiation. BT001254 mRNA Translation: AAN71010.1 BT001351 mRNA Translation: AAN71106.1 BT044258 mRNA Translation: ACH92323.1 |
RefSeqi | NP_001303438.1, NM_001316509.1 NP_651206.3, NM_142949.4 NP_732941.2, NM_170115.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0084533; FBpp0083918; FBgn0039141 FBtr0084534; FBpp0083919; FBgn0039141 |
GeneIDi | 42846 |
KEGGi | dme:Dmel_CG5977 |
UCSCi | CG5977-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF56223.3 AE014297 Genomic DNA Translation: AAN13975.2 AY069522 mRNA Translation: AAL39667.1 Different initiation. BT001254 mRNA Translation: AAN71010.1 BT001351 mRNA Translation: AAN71106.1 BT044258 mRNA Translation: ACH92323.1 |
RefSeqi | NP_001303438.1, NM_001316509.1 NP_651206.3, NM_142949.4 NP_732941.2, NM_170115.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3B9P | X-ray | 2.70 | A | 463-758 | [»] | |
6NYV | X-ray | 2.42 | B | 445-758 | [»] | |
6NYW | X-ray | 2.19 | B | 445-758 | [»] | |
6P10 | X-ray | 2.30 | B | 445-758 | [»] | |
6P11 | X-ray | 2.15 | B | 445-758 | [»] | |
6P12 | X-ray | 1.94 | B | 445-758 | [»] | |
6P13 | X-ray | 2.10 | B | 445-758 | [»] | |
6P14 | X-ray | 1.93 | A | 445-758 | [»] | |
AlphaFoldDBi | Q8I0P1 | |||||
SMRi | Q8I0P1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 67781, 6 interactors |
DIPi | DIP-59834N |
IntActi | Q8I0P1, 2 interactors |
STRINGi | 7227.FBpp0083918 |
PTM databases
iPTMneti | Q8I0P1 |
Proteomic databases
PaxDbi | Q8I0P1 |
PRIDEi | Q8I0P1 |
Protocols and materials databases
DNASUi | 42846 |
Genome annotation databases
EnsemblMetazoai | FBtr0084533; FBpp0083918; FBgn0039141 FBtr0084534; FBpp0083919; FBgn0039141 |
GeneIDi | 42846 |
KEGGi | dme:Dmel_CG5977 |
UCSCi | CG5977-RA, d. melanogaster |
Organism-specific databases
CTDi | 42846 |
FlyBasei | FBgn0039141, spas |
VEuPathDBi | VectorBase:FBgn0039141 |
Phylogenomic databases
eggNOGi | KOG0740, Eukaryota |
GeneTreei | ENSGT00940000156258 |
InParanoidi | Q8I0P1 |
OMAi | GMTNEPM |
PhylomeDBi | Q8I0P1 |
Enzyme and pathway databases
BRENDAi | 5.6.1.1, 1994 |
Reactomei | R-DME-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III |
Miscellaneous databases
BioGRID-ORCSi | 42846, 0 hits in 1 CRISPR screen |
EvolutionaryTracei | Q8I0P1 |
GenomeRNAii | 42846 |
PROi | PR:Q8I0P1 |
Gene expression databases
Bgeei | FBgn0039141, Expressed in female gonad and 54 other tissues |
ExpressionAtlasi | Q8I0P1, baseline and differential |
Genevisiblei | Q8I0P1, DM |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
HAMAPi | MF_03021, Spastin, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR007330, MIT_dom IPR027417, P-loop_NTPase IPR017179, Spastin |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00745, MIT, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPAST_DROME | |
Accessioni | Q8I0P1Primary (citable) accession number: Q8I0P1 Secondary accession number(s): Q8IMX5, Q8T066 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | March 24, 2009 | |
Last modified: | May 25, 2022 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families