UniProtKB - Q8HXQ5 (MRP1_BOVIN)
Multidrug resistance-associated protein 1
ABCC1
Functioni
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export (PubMed:12067707).
Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (By similarity).
Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity).
By similarity1 PublicationCatalytic activityi
- an S-substituted glutathione(in) + ATP + H2O = ADP + an S-substituted glutathione(out) + H+ + phosphateBy similarityEC:7.6.2.3By similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + sphing-4-enine 1-phosphate(in) = ADP + H+ + phosphate + sphing-4-enine 1-phosphate(out)By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 17β-estradiol 17-O-(β-D-glucuronate)(in) + ATP + H2O = 17β-estradiol 17-O-(β-D-glucuronate)(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2).1 Publication EC:7.6.2.2
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 678 – 685 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1326 – 1333 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type glutathione S-conjugate transporter activity Source: UniProtKB
- ABC-type transporter activity Source: UniProtKB
- ABC-type xenobiotic transporter activity Source: GO_Central
- ATPase-coupled lipid transmembrane transporter activity Source: Ensembl
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- carboxylic acid transmembrane transporter activity Source: Ensembl
- efflux transmembrane transporter activity Source: Ensembl
- glutathione transmembrane transporter activity Source: GO_Central
- hydrolase activity Source: UniProtKB-KW
- xenobiotic transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- export across plasma membrane Source: Ensembl
- glutathione transmembrane transport Source: GO_Central
- leukotriene transport Source: UniProtKB
- phospholipid translocation Source: Ensembl
- positive regulation of inflammatory response Source: UniProtKB
- response to xenobiotic stimulus Source: UniProtKB
- sphingolipid translocation Source: Ensembl
- transepithelial transport Source: Ensembl
- transmembrane transport Source: GO_Central
- xenobiotic transport Source: GO_Central
- xenobiotic transport across blood-brain barrier Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 7.6.2.2, 908 |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug resistance-associated protein 1By similarity (EC:7.6.2.21 Publication)Alternative name(s): ATP-binding cassette sub-family C member 1 Glutathione-S-conjugate-translocating ATPase ABCC1By similarity (EC:7.6.2.3By similarity) Leukotriene C(4) transporter Short name: LTC4 transporter |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000021090 |
VGNCi | VGNC:25468, ABCC1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein PROSITE-ProRule annotation
Plasma Membrane
- apical plasma membrane Source: Ensembl
- basolateral plasma membrane Source: GO_Central
- lateral plasma membrane Source: Ensembl
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | ExtracellularBy similarityAdd BLAST | 33 | |
Transmembranei | 34 – 54 | Helical; Name=1PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 55 – 74 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 75 – 95 | Helical; Name=2PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 96 – 100 | ExtracellularBy similarity | 5 | |
Transmembranei | 101 – 121 | Helical; Name=3PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 122 – 133 | CytoplasmicBy similarityAdd BLAST | 12 | |
Transmembranei | 134 – 154 | Helical; Name=4PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 155 – 172 | ExtracellularBy similarityAdd BLAST | 18 | |
Transmembranei | 173 – 193 | Helical; Name=5PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 194 – 316 | CytoplasmicBy similarityAdd BLAST | 123 | |
Transmembranei | 317 – 337 | Helical; Name=6PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 338 – 363 | ExtracellularBy similarityAdd BLAST | 26 | |
Transmembranei | 364 – 384 | Helical; Name=7PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 385 – 440 | CytoplasmicBy similarityAdd BLAST | 56 | |
Transmembranei | 441 – 461 | Helical; Name=8PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 462 – 464 | ExtracellularBy similarity | 3 | |
Transmembranei | 465 – 485 | Helical; Name=9PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 486 – 547 | CytoplasmicBy similarityAdd BLAST | 62 | |
Transmembranei | 548 – 568 | Helical; Name=10PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 569 – 590 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 591 – 611 | Helical; Name=11PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 612 – 966 | CytoplasmicBy similarityAdd BLAST | 355 | |
Transmembranei | 967 – 987 | Helical; Name=12PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 988 – 1024 | ExtracellularBy similarityAdd BLAST | 37 | |
Transmembranei | 1025 – 1045 | Helical; Name=13PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1046 – 1088 | CytoplasmicBy similarityAdd BLAST | 43 | |
Transmembranei | 1089 – 1109 | Helical; Name=14PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1110 | ExtracellularBy similarity | 1 | |
Transmembranei | 1111 – 1131 | Helical; Name=15PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1132 – 1202 | CytoplasmicBy similarityAdd BLAST | 71 | |
Transmembranei | 1203 – 1223 | Helical; Name=16PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1224 – 1225 | ExtracellularBy similarity | 2 | |
Transmembranei | 1226 – 1246 | Helical; Name=17PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1247 – 1530 | CytoplasmicBy similarityAdd BLAST | 284 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093349 | 1 – 1530 | Multidrug resistance-associated protein 1Add BLAST | 1530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 19 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 277 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 289 | PhosphoserineBy similarity | 1 | |
Modified residuei | 503 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 915 | PhosphoserineBy similarity | 1 | |
Modified residuei | 930 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 1005 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q8HXQ5 |
PRIDEi | Q8HXQ5 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q8HXQ5, baseline and differential |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8HXQ5 |
SMRi | Q8HXQ5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 325 – 608 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 284 | |
Domaini | 644 – 868 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 225 | |
Domaini | 974 – 1255 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 282 | |
Domaini | 1292 – 1526 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 235 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 912 – 939 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 912 – 927 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0054, Eukaryota |
GeneTreei | ENSGT00940000160271 |
InParanoidi | Q8HXQ5 |
OMAi | IRYDFTP |
OrthoDBi | 138195at2759 |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR005292, Multidrug-R_assoc IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
TIGRFAMsi | TIGR00957, MRP_assoc_pro, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MALRDFCSVD GSDLFWEWNV TWNTSNPDFT KCFQNTVLVW VPCSYLWVCF
60 70 80 90 100
PFYFLYLSHH DRGYIQMTHL NKAKTALGFL LWIVCWADLF YSFWERSMGK
110 120 130 140 150
LLAPVFLVSP TLLGITMLLA TFLIQIERRR GVQSSGIMLT FWLIALLCAL
160 170 180 190 200
AILRSKIMTA LKEDARVDVF RDVTFYIYFS LVLIQLVLSC FSDRSPLFSE
210 220 230 240 250
TINDPNPCPE SSASFLSRIT FWWITGMMVQ GYRQPLESTD LWSLNKEDTS
260 270 280 290 300
EQVVPVLVKN WKKECAKSRK QPVKIVYSSK DPAKPKGSSK VDVNEEAEAL
310 320 330 340 350
IVKCPQKERD PSLFKVLYKT FGPYFLMSFL FKAVHDLMMF AGPEILKLLI
360 370 380 390 400
NFVNDKKAPE WQGYFYTALL FISACLQTLV LHQYFHICFV SGMRIKTAVI
410 420 430 440 450
GAVYRKALVI TNAARKSSTV GEIVNLMSVD AQRFMDLATY INMIWSAPLQ
460 470 480 490 500
VILALYLLWL NLGPSVLAGV AVMVLMVPLN AVMAMKTKTY QVAHMKSKDN
510 520 530 540 550
RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK KSAYLAAVGT
560 570 580 590 600
FTWVCTPFLV ALSTFAVYVT VDENNILDAQ KAFVSLALFN ILRFPLNILP
610 620 630 640 650
MVISSIVQAS VSLKRLRVFL SHEDLDPDSI QRRPIKDAGA TNSITVKNAT
660 670 680 690 700
FTWARNDPPT LHGITFSVPE GSLVAVVGQV GCGKSSLLSA LLAEMDKVEG
710 720 730 740 750
HVTVKGSVAY VPQQAWIQNI SLRENILFGR QLQERYYKAV VEACALLPDL
760 770 780 790 800
EILPSGDRTE IGEKGVNLSG GQKQRVSLAR AVYCDSDVYL LDDPLSAVDA
810 820 830 840 850
HVGKHIFENV IGPKGLLKNK TRLLVTHAIS YLPQMDVIIV MSGGKISEMG
860 870 880 890 900
SYQELLARDG AFAEFLRTYA SAEQEQGQPE DGLAGVGGPG KEVKQMENGM
910 920 930 940 950
LVTDTAGKQM QRQLSSSSSY SRDVSQHHTS TAELRKPGPT EETWKLVEAD
960 970 980 990 1000
KAQTGQVKLS VYWDYMKAIG LFISFLSIFL FLCNHVASLV SNYWLSLWTD
1010 1020 1030 1040 1050
DPIVNGTQEH TQVRLSVYGA LGISQGITVF GYSMAVSIGG IFASRRLHLD
1060 1070 1080 1090 1100
LLHNVLRSPI SFFERTPSGN LVNRFSKELD TVDSMIPQVI KMFMGSLFNV
1110 1120 1130 1140 1150
IGACIIILLA TPMAAVIIPP LGLIYFFVQR FYVASSRQLK RLESVSRSPV
1160 1170 1180 1190 1200
YSHFNETLLG VSVIRAFEEQ ERFIRQSDLK VDENQKAYYP SIVANRWLAV
1210 1220 1230 1240 1250
RLECVGNCIV LFASLFAVIS RHSLSAGLVG LSVSYSLQVT TYLNWLVRMS
1260 1270 1280 1290 1300
SEMETNIVAV ERLKEYSETE KEAPWQIQDM APPKDWPQVG RVEFRDYGLR
1310 1320 1330 1340 1350
YREDLDLVLK HINVTIDGGE KVGIVGRTGA GKSSLTLGLF RIKESAEGEI
1360 1370 1380 1390 1400
IIDDINIAKI GLHDLRFKIT IIPQDPVLFS GSLRMNLDPF SQYSDEEVWT
1410 1420 1430 1440 1450
SLELAHLKGF VSALPDKLNH ECAEGGENLS VGQRQLVCLA RALLRKTKIL
1460 1470 1480 1490 1500
VLDEATAAVD LETDDLIQST IRTQFDDCTV LTIAHRLNTI MDYTRVIVLD
1510 1520 1530
KGEIQEWGSP SDLLQQRGLF YSMAKDSGLV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1MY93 | A0A3Q1MY93_BOVIN | ABC-type glutathione-S-conjugate tr... | ABCC1 | 1,506 | Annotation score: | ||
A0A3Q1LMX5 | A0A3Q1LMX5_BOVIN | ABC-type glutathione-S-conjugate tr... | ABCC1 | 1,464 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB082124 mRNA Translation: BAC15550.1 |
RefSeqi | NP_776648.1, NM_174223.1 |
Genome annotation databases
Ensembli | ENSBTAT00000028094; ENSBTAP00000028094; ENSBTAG00000021090 |
GeneIDi | 281588 |
KEGGi | bta:281588 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB082124 mRNA Translation: BAC15550.1 |
RefSeqi | NP_776648.1, NM_174223.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5UJ9 | electron microscopy | 3.49 | A | 205-1530 | [»] | |
5UJA | electron microscopy | 3.34 | A | 205-1530 | [»] | |
6BHU | electron microscopy | 3.14 | A | 1-1530 | [»] | |
6UY0 | electron microscopy | 3.23 | A | 1-1530 | [»] | |
AlphaFoldDBi | Q8HXQ5 | |||||
SMRi | Q8HXQ5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000028094 |
Proteomic databases
PaxDbi | Q8HXQ5 |
PRIDEi | Q8HXQ5 |
Genome annotation databases
Ensembli | ENSBTAT00000028094; ENSBTAP00000028094; ENSBTAG00000021090 |
GeneIDi | 281588 |
KEGGi | bta:281588 |
Organism-specific databases
CTDi | 4363 |
VEuPathDBi | HostDB:ENSBTAG00000021090 |
VGNCi | VGNC:25468, ABCC1 |
Phylogenomic databases
eggNOGi | KOG0054, Eukaryota |
GeneTreei | ENSGT00940000160271 |
InParanoidi | Q8HXQ5 |
OMAi | IRYDFTP |
OrthoDBi | 138195at2759 |
Enzyme and pathway databases
BRENDAi | 7.6.2.2, 908 |
Gene expression databases
ExpressionAtlasi | Q8HXQ5, baseline and differential |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR005292, Multidrug-R_assoc IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
TIGRFAMsi | TIGR00957, MRP_assoc_pro, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | MRP1_BOVIN | |
Accessioni | Q8HXQ5Primary (citable) accession number: Q8HXQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families