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UniProtKB - Q8H3I4 (HAOX_ORYSJ)
Protein
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4
Gene
GLO4
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Oxidase that catalyzes the oxidation of a broad range of 2-hydroxyacids to the corresponding 2-oxoacids, with a reduction of O2 to H2O2. May be involved in a general medium- and long-chain fatty acid catabolic pathway such as alpha-oxidation.
By similarityCatalytic activityi
Cofactori
FMNBy similarity
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 27 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 | |
Binding sitei | 109 | FMNBy similarity | 1 | |
Binding sitei | 132 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 | |
Binding sitei | 158 | FMNBy similarity | 1 | |
Binding sitei | 167 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 | |
Binding sitei | 231 | FMNBy similarity | 1 | |
Binding sitei | 253 | FMNBy similarity | 1 | |
Active sitei | 255 | Proton acceptorBy similarity | 1 | |
Binding sitei | 258 | 2-oxocarboxylatePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 80 – 82 | FMNBy similarity | 3 | |
Nucleotide bindingi | 130 – 132 | FMNBy similarity | 3 | |
Nucleotide bindingi | 286 – 290 | FMNBy similarity | 5 | |
Nucleotide bindingi | 309 – 310 | FMNBy similarity | 2 |
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: UniProtKB
- FMN binding Source: InterPro
GO - Biological processi
- cellular response to virus Source: UniProtKB
- oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
- photorespiration Source: UniProtKB
- regulation of photosynthesis Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
PlantReactomei | R-OSA-1119312, Photorespiration R-OSA-1119596, Glutamate biosynthesis I |
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (EC:1.1.3.15By similarity)Alternative name(s): Glycolate oxidase 4 Short name: GOX 4 Short name: OsGLO4 Short chain alpha-hydroxy acid oxidase GLO4 |
Gene namesi | Name:GLO4 Ordered Locus Names:Os07g0616500 ORF Names:B1056G08.112, OsJ_25131 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Peroxisome
- peroxisome Source: UniProtKB
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000403415 | 1 – 366 | Peroxisomal (S)-2-hydroxy-acid oxidase GLO4Add BLAST | 366 |
Proteomic databases
PaxDbi | Q8H3I4 |
Interactioni
Subunit structurei
Homotetramer (By similarity). Binds to CATB and CATC; these interactions are disturbed by alpha-hydroxy-2-pyridinemethanesulfonic acid (HPMS) and salicylic acid (SA) (PubMed:26900141).
By similarityProtein-protein interaction databases
STRINGi | 4530.OS07T0616500-01 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 360 | FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST | 360 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 364 – 366 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
HOGENOMi | CLU_020639_0_0_1 |
InParanoidi | Q8H3I4 |
OMAi | FQYEIYL |
OrthoDBi | 879633at2759 |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8H3I4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDNLPVNVR EYQELAKKAL PKMAYDYING GAEDEHTLRE NIAAYTRIIL
60 70 80 90 100
RPRVLVDVSK IDMSTTLLGY TMRSPIIVAP TGGHKLAHPE GEKATARAAA
110 120 130 140 150
SCNAIMVLSF SSSCKIEDVA SSCNAIRFYQ LYVYKNRNVS ATLVRRAESC
160 170 180 190 200
GFKALLLTVD TPMLGRREAD IRNKMVFPRS GNLEGLMTTD DHDTTNGSQL
210 220 230 240 250
ERFARATLDP SLSWKDIEWL KSITSMPIFL KGIVTAEDAR RAVEAGVAGV
260 270 280 290 300
IVSNHGARQL DYAPATIAAL EEVVRAVAGA VPVLVDGGIR RGTDVFKALA
310 320 330 340 350
LGARAVMVGR PVFFGLAARG EAGARHVIEM LNGELEVAMA LCGCRSVGEI
360
TRSHVMTEGD RIRSLL
Sequence cautioni
The sequence EEE67592 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 313 | F → L in EEE67592 (PubMed:15685292).Curated | 1 | |
Sequence conflicti | 321 | E → D in EEE67592 (PubMed:15685292).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP004988 Genomic DNA Translation: BAC79990.1 AP008213 Genomic DNA Translation: BAF22190.1 AP014963 Genomic DNA Translation: BAT02654.1 CM000144 Genomic DNA Translation: EEE67592.1 Frameshift. AK071738 mRNA Translation: BAG92663.1 |
RefSeqi | XP_015647067.1, XM_015791581.1 XP_015647068.1, XM_015791582.1 XP_015647069.1, XM_015791583.1 XP_015647070.1, XM_015791584.1 |
Genome annotation databases
EnsemblPlantsi | Os07t0616500-01; Os07t0616500-01; Os07g0616500 |
GeneIDi | 4343908 |
Gramenei | Os07t0616500-01; Os07t0616500-01; Os07g0616500 |
KEGGi | osa:4343908 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP004988 Genomic DNA Translation: BAC79990.1 AP008213 Genomic DNA Translation: BAF22190.1 AP014963 Genomic DNA Translation: BAT02654.1 CM000144 Genomic DNA Translation: EEE67592.1 Frameshift. AK071738 mRNA Translation: BAG92663.1 |
RefSeqi | XP_015647067.1, XM_015791581.1 XP_015647068.1, XM_015791582.1 XP_015647069.1, XM_015791583.1 XP_015647070.1, XM_015791584.1 |
3D structure databases
SMRi | Q8H3I4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS07T0616500-01 |
Proteomic databases
PaxDbi | Q8H3I4 |
Genome annotation databases
EnsemblPlantsi | Os07t0616500-01; Os07t0616500-01; Os07g0616500 |
GeneIDi | 4343908 |
Gramenei | Os07t0616500-01; Os07t0616500-01; Os07g0616500 |
KEGGi | osa:4343908 |
Phylogenomic databases
eggNOGi | KOG0538, Eukaryota |
HOGENOMi | CLU_020639_0_0_1 |
InParanoidi | Q8H3I4 |
OMAi | FQYEIYL |
OrthoDBi | 879633at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
PlantReactomei | R-OSA-1119312, Photorespiration R-OSA-1119596, Glutamate biosynthesis I |
Gene expression databases
Genevisiblei | Q8H3I4, OS |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HAOX_ORYSJ | |
Accessioni | Q8H3I4Primary (citable) accession number: Q8H3I4 Secondary accession number(s): A0A0P0X8U9, B9FU85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2011 |
Last sequence update: | October 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families