GLO4

Functionⁱ

Photorespiratory enzyme that can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Not required for oxalate accumulation (By similarity).By similarity

Catalytic activityⁱ

(S)-2-hydroxy acid + O₂ = 2-oxo acid + H₂O₂.

Cofactorⁱ

FMNPROSITE-ProRule annotation

Pathwayⁱ: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:

no protein annotated in this organism
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4), Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5), Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 (GLO2), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3)
no protein annotated in this organism

This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	27	SubstratePROSITE-ProRule annotation	1
Binding siteⁱ	109	FMNPROSITE-ProRule annotation	1
Binding siteⁱ	130	FMNPROSITE-ProRule annotation	1
Binding siteⁱ	132	SubstratePROSITE-ProRule annotation	1
Binding siteⁱ	158	FMNPROSITE-ProRule annotation	1
Binding siteⁱ	167	SubstratePROSITE-ProRule annotation	1
Binding siteⁱ	231	FMNPROSITE-ProRule annotation	1
Active siteⁱ	255	Proton acceptorPROSITE-ProRule annotation	1
Binding siteⁱ	258	SubstratePROSITE-ProRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	286 – 310	FMNPROSITE-ProRule annotationAdd BLAST		25

GO - Molecular functionⁱ

FMN binding Source: InterPro
glycolate oxidase activity Source: UniProtKB
long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

modulation by virus of host morphology or physiology Source: UniProtKB
oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
photorespiration Source: UniProtKB
regulation of photosynthesis Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Glycolate pathway, Host-virus interaction, Photorespiration
Ligand	Flavoprotein, FMN

Enzyme and pathway databases

Reactomeⁱ	R-OSA-389661 Glyoxylate metabolism and glycine degradation R-OSA-390918 Peroxisomal lipid metabolism
UniPathwayⁱ	UPA00951; UER00912

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (EC:1.1.3.15) Alternative name(s): Glycolate oxidase 4 Short name: GOX 4 Short name: OsGLO4 Short chain alpha-hydroxy acid oxidase GLO4
Gene namesⁱ	Name:GLO4 Ordered Locus Names:Os07g0616500 ORF Names:B1056G08.112, OsJ_25131
Organismⁱ	Oryza sativa subsp. japonica (Rice)
Taxonomic identifierⁱ	39947 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › Liliopsida › Petrosaviidae › commelinids › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa
Proteomesⁱ	UP000059680 Componentⁱ: Chromosome 7

Keywords - Cellular componentⁱ

Peroxisome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000403415	1 – 366	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4Add BLAST		366

Proteomic databases

PaxDbⁱ	Q8H3I4
PRIDEⁱ	Q8H3I4

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q8H3I4 differential
Genevisibleⁱ	Q8H3I4 OS

Interactionⁱ

Subunit structureⁱ

Homotetramer or homooctamer. Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes (By similarity).By similarity

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os07g42440.1

STRINGⁱ

39947.LOC_Os07g42440.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8H3I4
SMRⁱ	Q8H3I4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 360	FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST		360

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	364 – 366	Microbody targeting signalSequence analysis		3

Sequence similaritiesⁱ

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG0538 Eukaryota COG1304 LUCA
InParanoidⁱ	Q8H3I4
KEGG Orthology (KO) More...KOⁱ	K11517
OMAⁱ	NEFHTSM
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360EDE

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02809 alpha_hydroxyacid_oxid_FMN, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR012133 Alpha-hydoxy_acid_DH_FMN IPR000262 FMN-dep_DH IPR037396 FMN_HAD IPR008259 FMN_hydac_DH_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01070 FMN_dh, 1 hit
PIRSFⁱ	PIRSF000138 Al-hdrx_acd_dh, 1 hit
PROSITEⁱ	View protein in PROSITE PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8H3I4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEDNLPVNVR EYQELAKKAL PKMAYDYING GAEDEHTLRE NIAAYTRIIL 
        60         70         80         90        100
RPRVLVDVSK IDMSTTLLGY TMRSPIIVAP TGGHKLAHPE GEKATARAAA 
       110        120        130        140        150
SCNAIMVLSF SSSCKIEDVA SSCNAIRFYQ LYVYKNRNVS ATLVRRAESC 
       160        170        180        190        200
GFKALLLTVD TPMLGRREAD IRNKMVFPRS GNLEGLMTTD DHDTTNGSQL 
       210        220        230        240        250
ERFARATLDP SLSWKDIEWL KSITSMPIFL KGIVTAEDAR RAVEAGVAGV 
       260        270        280        290        300
IVSNHGARQL DYAPATIAAL EEVVRAVAGA VPVLVDGGIR RGTDVFKALA 
       310        320        330        340        350
LGARAVMVGR PVFFGLAARG EAGARHVIEM LNGELEVAMA LCGCRSVGEI 
       360 
TRSHVMTEGD RIRSLL

Length:366

Mass (Da):39,764

Last modified:October 1, 2003 - v2

Checksum:ⁱ73FCBAD72CC9B9F5

GO

Sequence cautionⁱ

The sequence EEE67592 differs from that shown. Reason: Frameshift at position 321.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	313	F → L in EEE67592 (PubMed:15685292).Curated		1
Sequence conflictⁱ	321	E → D in EEE67592 (PubMed:15685292).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP004988 Genomic DNA Translation: BAC79990.1 AP008213 Genomic DNA Translation: BAF22190.1 AP014963 Genomic DNA Translation: BAT02654.1 CM000144 Genomic DNA Translation: EEE67592.1 Frameshift. AK071738 mRNA Translation: BAG92663.1
NCBI Reference Sequences More...RefSeqⁱ	XP_015647067.1, XM_015791581.1 XP_015647068.1, XM_015791582.1 XP_015647069.1, XM_015791583.1 XP_015647070.1, XM_015791584.1
UniGeneⁱ	Os.8126

Genome annotation databases

EnsemblPlantsⁱ	Os07t0616500-01; Os07t0616500-01; Os07g0616500
GeneIDⁱ	4343908
Grameneⁱ	Os07t0616500-01; Os07t0616500-01; Os07g0616500
KEGGⁱ	osa:4343908

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q8H3I4	Uncharacterized protein		ORYNI		383
Uncharacterized protein		Oryza glumipatula		383
Uncharacterized protein		ORYGL		366
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4		ORYSI		366

Protein	Similar proteins		Species	Score	Length
Q8H3I4	Uncharacterized protein		ORYNI		383
Uncharacterized protein		Oryza glumipatula		383
Uncharacterized protein		ORYGL		366
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4		ORYSI		366
UPI0003EACE94		ORYBR		367
+227

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP004988 Genomic DNA Translation: BAC79990.1 AP008213 Genomic DNA Translation: BAF22190.1 AP014963 Genomic DNA Translation: BAT02654.1 CM000144 Genomic DNA Translation: EEE67592.1 Frameshift. AK071738 mRNA Translation: BAG92663.1
NCBI Reference Sequences More...RefSeqⁱ	XP_015647067.1, XM_015791581.1 XP_015647068.1, XM_015791582.1 XP_015647069.1, XM_015791583.1 XP_015647070.1, XM_015791584.1
UniGeneⁱ	Os.8126

3D structure databases

ProteinModelPortalⁱ	Q8H3I4
SMRⁱ	Q8H3I4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os07g42440.1

STRINGⁱ

39947.LOC_Os07g42440.1

Proteomic databases

PaxDbⁱ	Q8H3I4
PRIDEⁱ	Q8H3I4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	Os07t0616500-01; Os07t0616500-01; Os07g0616500
GeneIDⁱ	4343908
Grameneⁱ	Os07t0616500-01; Os07t0616500-01; Os07g0616500
KEGGⁱ	osa:4343908

Phylogenomic databases

eggNOGⁱ	KOG0538 Eukaryota COG1304 LUCA
InParanoidⁱ	Q8H3I4
KEGG Orthology (KO) More...KOⁱ	K11517
OMAⁱ	NEFHTSM
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360EDE

Enzyme and pathway databases

UniPathwayⁱ	UPA00951; UER00912
Reactomeⁱ	R-OSA-389661 Glyoxylate metabolism and glycine degradation R-OSA-390918 Peroxisomal lipid metabolism

Gene expression databases

ExpressionAtlasⁱ	Q8H3I4 differential
Genevisibleⁱ	Q8H3I4 OS

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02809 alpha_hydroxyacid_oxid_FMN, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR012133 Alpha-hydoxy_acid_DH_FMN IPR000262 FMN-dep_DH IPR037396 FMN_HAD IPR008259 FMN_hydac_DH_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01070 FMN_dh, 1 hit
PIRSFⁱ	PIRSF000138 Al-hdrx_acd_dh, 1 hit
PROSITEⁱ	View protein in PROSITE PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLO4_ORYSJ
Accessionⁱ	Q8H3I4Primary (citable) accession number: Q8H3I4 Secondary accession number(s): A0A0P0X8U9, B9FU85
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
	Last sequence update:	October 1, 2003
	Last modified:	May 23, 2018
	This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8H3I4 (GLO4_ORYSJ)

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Peroxisomal (S)-2-hydroxy-acid oxidase GLO4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: photorespiration

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Peroxisome

Peroxisome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: photorespiration

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ