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Protein

Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial

Gene

IAR4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Enzyme regulationi

E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.By similarity

GO - Molecular functioni

  • cobalt ion binding Source: TAIR
  • pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC
  • zinc ion binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciARA:AT1G24180-MONOMER
ReactomeiR-ATH-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-ATH-5362517 Signaling by Retinoic Acid
R-ATH-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial (EC:1.2.4.1)
Short name:
PDHE1-A
Alternative name(s):
Protein IAA-CONJUGATE-RESISTANT 4
Gene namesi
Name:IAR4
Ordered Locus Names:At1g24180
ORF Names:F3I6.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G24180
TAIRilocus:2032367 AT1G24180

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Reduced sensitivity to several IAA-amino acid conjugates.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi121R → C in iar4-1; reduced sensitivity to several IAA-amino acid conjugates. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionSequence analysisAdd BLAST28
ChainiPRO_000026002429 – 393Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrialAdd BLAST365

Proteomic databases

PaxDbiQ8H1Y0
PRIDEiQ8H1Y0

PTM databases

iPTMnetiQ8H1Y0

Expressioni

Gene expression databases

ExpressionAtlasiQ8H1Y0 differential
GenevisibleiQ8H1Y0 AT

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta subunits.By similarity

Protein-protein interaction databases

BioGridi24269, 1 interactor
STRINGi3702.AT1G24180.1

Structurei

3D structure databases

ProteinModelPortaliQ8H1Y0
SMRiQ8H1Y0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0225 Eukaryota
COG1071 LUCA
HOGENOMiHOG000281336
InParanoidiQ8H1Y0
KOiK00161
OMAiGWGGSMH
OrthoDBiEOG09360B4J
PhylomeDBiQ8H1Y0

Family and domain databases

InterProiView protein in InterPro
IPR001017 DH_E1
IPR017597 Pyrv_DH_E1_asu_subgrp-y
IPR029061 THDP-binding
PfamiView protein in Pfam
PF00676 E1_dh, 1 hit
SUPFAMiSSF52518 SSF52518, 1 hit
TIGRFAMsiTIGR03182 PDH_E1_alph_y, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8H1Y0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSRLSSRS NTFLKPAITA LPSSIRRHVS TDSSPITIET AVPFTSHLCE
60 70 80 90 100
SPSRSVETSS EEILAFFRDM ARMRRMEIAA DSLYKAKLIR GFCHLYDGQE
110 120 130 140 150
ALAVGMEAAI TKKDAIITSY RDHCTFIGRG GKLVDAFSEL MGRKTGCSHG
160 170 180 190 200
KGGSMHFYKK DASFYGGHGI VGAQIPLGCG LAFAQKYNKD EAVTFALYGD
210 220 230 240 250
GAANQGQLFE ALNISALWDL PAILVCENNH YGMGTATWRS AKSPAYFKRG
260 270 280 290 300
DYVPGLKVDG MDALAVKQAC KFAKEHALKN GPIILEMDTY RYHGHSMSDP
310 320 330 340 350
GSTYRTRDEI SGVRQVRDPI ERVRKLLLTH DIATEKELKD MEKEIRKEVD
360 370 380 390
DAVAQAKESP IPDASELFTN MYVKDCGVES FGADRKELKV TLP
Length:393
Mass (Da):43,359
Last modified:November 28, 2006 - v2
Checksum:i1B28971865B52817
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231Y → D in AAN15218 (PubMed:15173569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135561 mRNA Translation: AAN15218.1
AC002396 Genomic DNA Translation: AAC00577.1
CP002684 Genomic DNA Translation: AEE30492.1
AF360215 mRNA Translation: AAK25925.1
AY051018 mRNA Translation: AAK93695.1
AY088101 mRNA Translation: AAM65647.1
PIRiT00648
RefSeqiNP_173828.1, NM_102264.5
UniGeneiAt.24830

Genome annotation databases

EnsemblPlantsiAT1G24180.1; AT1G24180.1; AT1G24180
GeneIDi839031
GrameneiAT1G24180.1; AT1G24180.1; AT1G24180
KEGGiath:AT1G24180

Similar proteinsi

Entry informationi

Entry nameiODPA2_ARATH
AccessioniPrimary (citable) accession number: Q8H1Y0
Secondary accession number(s): O48685
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: May 23, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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