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UniProtKB - Q8H1S0 (MIOX4_ARATH)
Protein
Inositol oxygenase 4
Gene
MIOX4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the oxygenative cleavage of myo-inositol to D-glucuronate. Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.
1 Publication1 PublicationCatalytic activityi
Cofactori
Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity
: myo-inositol degradation into D-glucuronate Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glucuronate from myo-inositol. This subpathway is part of the pathway myo-inositol degradation into D-glucuronate, which is itself part of Polyol metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucuronate from myo-inositol, the pathway myo-inositol degradation into D-glucuronate and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 58 | SubstrateBy similarity | 1 | |
Metal bindingi | 128 | Iron 1By similarity | 1 | |
Metal bindingi | 153 | Iron 1By similarity | 1 | |
Metal bindingi | 154 | Iron 1By similarity | 1 | |
Metal bindingi | 154 | Iron 2By similarity | 1 | |
Binding sitei | 157 | SubstrateBy similarity | 1 | |
Metal bindingi | 226 | Iron 2By similarity | 1 | |
Metal bindingi | 252 | Iron 2By similarity | 1 | |
Metal bindingi | 285 | Iron 1By similarity | 1 |
GO - Molecular functioni
- inositol oxygenase activity Source: TAIR
- iron ion binding Source: InterPro
GO - Biological processi
- inositol catabolic process Source: GO_Central
- L-ascorbic acid biosynthetic process Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Ascorbate biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.13.99.1, 399 |
UniPathwayi | UPA00111;UER00527 |
Names & Taxonomyi
Protein namesi | Recommended name: Inositol oxygenase 4 (EC:1.13.99.11 Publication)Alternative name(s): Myo-inositol oxygenase 41 Publication Short name: AtMIOX41 Publication Short name: MI oxygenase 4 |
Gene namesi | Name:MIOX4 Ordered Locus Names:At4g26260 ORF Names:T25K17.70 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G26260 |
TAIRi | locus:2136839, AT4G26260 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079156 | 1 – 317 | Inositol oxygenase 4Add BLAST | 317 |
Proteomic databases
PaxDbi | Q8H1S0 |
ProteomicsDBi | 250706 [Q8H1S0-1] |
Expressioni
Tissue specificityi
Expressed in flowers, leaves, siliques, and to a lesser extent in roots.2 Publications
Gene expression databases
ExpressionAtlasi | Q8H1S0, baseline and differential |
Genevisiblei | Q8H1S0, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 14019, 1 interactor |
STRINGi | 3702.AT4G26260.2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 115 – 117 | Substrate bindingBy similarity | 3 | |
Regioni | 174 – 175 | Substrate bindingBy similarity | 2 | |
Regioni | 252 – 253 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the myo-inositol oxygenase family.Curated
Phylogenomic databases
eggNOGi | KOG1573, Eukaryota |
HOGENOMi | CLU_050259_2_0_1 |
InParanoidi | Q8H1S0 |
OMAi | GHEIYGT |
PhylomeDBi | Q8H1S0 |
Family and domain databases
InterProi | View protein in InterPro IPR007828, Inositol_oxygenase |
PANTHERi | PTHR12588, PTHR12588, 1 hit |
Pfami | View protein in Pfam PF05153, MIOX, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8H1S0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTISVEKPIF EEVSAFEKSG DNIGELKLDG GFSMPKMDTN DDEAFLAPEM
60 70 80 90 100
NAFGRQFRDY DVESERQKGV EEFYRLQHIN QTVDFVKKMR AEYGKLDKMV
110 120 130 140 150
MSIWECCELL NEVVDESDPD LDEPQIQHLL QSAEAIRKDY PNEDWLHLTA
160 170 180 190 200
LIHDLGKVIT LPQFGGLPQW AVVGDTFPVG CAFDESNVHH KYFVENPDFH
210 220 230 240 250
NETYNTKNGI YSEGCGLNNV MMSWGHDDYM YLVAKENGST LPSAGQFIIR
260 270 280 290 300
YHSFYPLHTA GEYTHLMNEE DKENLKWLHV FNKYDLYSKS KVHVDVEKVK
310
PYYMSLIKKY FPENLRW
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 77 | Q → R in AAP59548 (PubMed:14976233).Curated | 1 | |
Sequence conflicti | 300 | K → E in AAP59548 (PubMed:14976233).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041591 | 10 | F → FE in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY232552 mRNA Translation: AAP59548.1 AL049171 Genomic DNA Translation: CAB38955.1 AL161564 Genomic DNA Translation: CAB79481.1 CP002687 Genomic DNA Translation: AEE85176.1 CP002687 Genomic DNA Translation: AEE85177.1 AY142501 mRNA Translation: AAN13052.1 |
PIRi | T06010 |
RefSeqi | NP_001190844.1, NM_001203915.2 [Q8H1S0-2] NP_194356.2, NM_118759.5 [Q8H1S0-1] |
Genome annotation databases
EnsemblPlantsi | AT4G26260.1; AT4G26260.1; AT4G26260 [Q8H1S0-1] AT4G26260.2; AT4G26260.2; AT4G26260 [Q8H1S0-2] |
GeneIDi | 828732 |
Gramenei | AT4G26260.1; AT4G26260.1; AT4G26260 [Q8H1S0-1] AT4G26260.2; AT4G26260.2; AT4G26260 [Q8H1S0-2] |
KEGGi | ath:AT4G26260 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY232552 mRNA Translation: AAP59548.1 AL049171 Genomic DNA Translation: CAB38955.1 AL161564 Genomic DNA Translation: CAB79481.1 CP002687 Genomic DNA Translation: AEE85176.1 CP002687 Genomic DNA Translation: AEE85177.1 AY142501 mRNA Translation: AAN13052.1 |
PIRi | T06010 |
RefSeqi | NP_001190844.1, NM_001203915.2 [Q8H1S0-2] NP_194356.2, NM_118759.5 [Q8H1S0-1] |
3D structure databases
AlphaFoldDBi | Q8H1S0 |
SMRi | Q8H1S0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 14019, 1 interactor |
STRINGi | 3702.AT4G26260.2 |
Proteomic databases
PaxDbi | Q8H1S0 |
ProteomicsDBi | 250706 [Q8H1S0-1] |
Genome annotation databases
EnsemblPlantsi | AT4G26260.1; AT4G26260.1; AT4G26260 [Q8H1S0-1] AT4G26260.2; AT4G26260.2; AT4G26260 [Q8H1S0-2] |
GeneIDi | 828732 |
Gramenei | AT4G26260.1; AT4G26260.1; AT4G26260 [Q8H1S0-1] AT4G26260.2; AT4G26260.2; AT4G26260 [Q8H1S0-2] |
KEGGi | ath:AT4G26260 |
Organism-specific databases
Araporti | AT4G26260 |
TAIRi | locus:2136839, AT4G26260 |
Phylogenomic databases
eggNOGi | KOG1573, Eukaryota |
HOGENOMi | CLU_050259_2_0_1 |
InParanoidi | Q8H1S0 |
OMAi | GHEIYGT |
PhylomeDBi | Q8H1S0 |
Enzyme and pathway databases
UniPathwayi | UPA00111;UER00527 |
BRENDAi | 1.13.99.1, 399 |
Miscellaneous databases
PROi | PR:Q8H1S0 |
Gene expression databases
ExpressionAtlasi | Q8H1S0, baseline and differential |
Genevisiblei | Q8H1S0, AT |
Family and domain databases
InterProi | View protein in InterPro IPR007828, Inositol_oxygenase |
PANTHERi | PTHR12588, PTHR12588, 1 hit |
Pfami | View protein in Pfam PF05153, MIOX, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MIOX4_ARATH | |
Accessioni | Q8H1S0Primary (citable) accession number: Q8H1S0 Secondary accession number(s): Q6XGZ9, Q9STQ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families