Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 110 (02 Jun 2021)
Sequence version 1 (01 Mar 2003)
Previous versions | rss
Add a publicationFeedback
Protein

Cytosolic Fe-S cluster assembly factor NBP35

Gene

NBP35

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development.

UniRule annotation2 Publications

Miscellaneous

Although plant and algal NBP35 proteins lack the characteristic CXXC motif in the C-terminus, thought to be required for Fe-S cluster binding, they can bind a [4Fe-4S] cluster in the C-terminus. Also, in this linage, no CFD1 partner protein homolog as found in other eukaryotes can be found.1 Publication

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterUniRule annotation2 PublicationsNote: Binds 3 [4Fe-4S] clusters per homodimer. Contains two stable clusters in the N-termini and one labile, bridging cluster between subunits of the homodimer.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi14Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi28Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi37Iron-sulfur 1 (4Fe-4S)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi67 – 74ATPUniRule annotation8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NBP35UniRule annotation
Alternative name(s):
Nucleotide binding protein 35
Short name:
AtNBP35
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NBP35UniRule annotation
Ordered Locus Names:At5g50960
ORF Names:K3K7.12
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

Organism-specific databases

Arabidopsis Information Portal

More...
Araporti
AT5G50960

The Arabidopsis Information Resource

More...
TAIRi
locus:2157328, AT5G50960

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi28C → G: Impairs [4Fe-4S] cluster binding. 1 Publication1
Mutagenesisi31C → G: Impairs [4Fe-4S] cluster binding. 1 Publication1
Mutagenesisi322C → G: Seems to promote [2Fe-2S] cluster binding in the C-terminus. 1 Publication1
Mutagenesisi328C → G: Seems to promote [2Fe-2S] cluster binding in the C-terminus. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004218671 – 350Cytosolic Fe-S cluster assembly factor NBP35Add BLAST350

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q8H1Q2

PRoteomics IDEntifications database

More...
PRIDEi
Q8H1Q2

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
251250

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q8H1Q2

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q8H1Q2, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q8H1Q2, AT

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer and homotetramer. Predominantly homodimeric.

UniRule annotation2 Publications

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
20415, 1 interactor

STRING: functional protein association networks

More...
STRINGi
3702.AT5G50960.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 30DisorderedSequence analysisAdd BLAST30

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3022, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_024839_0_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q8H1Q2

Identification of Orthologs from Complete Genome Data

More...
OMAi
EMDCQVG

Database of Orthologous Groups

More...
OrthoDBi
1166096at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q8H1Q2

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_02040, Mrp_NBP35, 1 hit
MF_03038, NUBP1, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR019591, Mrp/NBP35_ATP-bd
IPR000808, Mrp_CS
IPR028601, NUBP1/Nbp35
IPR027417, P-loop_NTPase
IPR033756, YlxH/NBP35

The PANTHER Classification System

More...
PANTHERi
PTHR23264, PTHR23264, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF10609, ParA, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540, SSF52540, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS01215, MRP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8H1Q2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MENGDIPEDA NEHCPGPQSE SAGKSDSCAG CPNQEACATA PKGPDPDLVA
60 70 80 90 100
IAERMSTVKH KILVLSGKGG VGKSTFSAQL SFALAGMDHQ VGLMDIDICG
110 120 130 140 150
PSIPKMLGLE GQEIHQSNLG WSPVYVEDNL GVMSIGFMLP NSDEAVIWRG
160 170 180 190 200
PRKNGLIKQF LKDVYWGEID YLVVDAPPGT SDEHISIVQY LLPTGIDGAI
210 220 230 240 250
IVTTPQEVSL IDVRKEVSFC KKVGVPVLGV VENMSGLSQP LKDVKFMKLA
260 270 280 290 300
TETGSSINVT EDVIACLRKN APELLDIVAC SEVFDSSGGG AERMCREMGV
310 320 330 340 350
PFLGKVPMDP QLCKAAEQGK SCFEDNKCLI SAPALKSIIQ KVVPSTVMTE
Length:350
Mass (Da):37,306
Last modified:March 1, 2003 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i69DD66DB069B85B8
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAB08742 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti343V → F in AAK26019 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB017063 Genomic DNA Translation: BAB08742.1 Sequence problems.
CP002688 Genomic DNA Translation: AED96017.1
AF360309 mRNA Translation: AAK26019.1
AY142635 mRNA Translation: AAN13093.1

NCBI Reference Sequences

More...
RefSeqi
NP_568748.1, NM_124475.5

Genome annotation databases

Ensembl plant genome annotation project

More...
EnsemblPlantsi
AT5G50960.1; AT5G50960.1; AT5G50960

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
835169

Gramene; a comparative resource for plants

More...
Gramenei
AT5G50960.1; AT5G50960.1; AT5G50960

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ath:AT5G50960

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017063 Genomic DNA Translation: BAB08742.1 Sequence problems.
CP002688 Genomic DNA Translation: AED96017.1
AF360309 mRNA Translation: AAK26019.1
AY142635 mRNA Translation: AAN13093.1
RefSeqiNP_568748.1, NM_124475.5

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi20415, 1 interactor
STRINGi3702.AT5G50960.1

PTM databases

iPTMnetiQ8H1Q2

Proteomic databases

PaxDbiQ8H1Q2
PRIDEiQ8H1Q2
ProteomicsDBi251250

Genome annotation databases

EnsemblPlantsiAT5G50960.1; AT5G50960.1; AT5G50960
GeneIDi835169
GrameneiAT5G50960.1; AT5G50960.1; AT5G50960
KEGGiath:AT5G50960

Organism-specific databases

AraportiAT5G50960
TAIRilocus:2157328, AT5G50960

Phylogenomic databases

eggNOGiKOG3022, Eukaryota
HOGENOMiCLU_024839_0_1_1
InParanoidiQ8H1Q2
OMAiEMDCQVG
OrthoDBi1166096at2759
PhylomeDBiQ8H1Q2

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q8H1Q2

Gene expression databases

ExpressionAtlasiQ8H1Q2, baseline and differential
GenevisibleiQ8H1Q2, AT

Family and domain databases

HAMAPiMF_02040, Mrp_NBP35, 1 hit
MF_03038, NUBP1, 1 hit
InterProiView protein in InterPro
IPR019591, Mrp/NBP35_ATP-bd
IPR000808, Mrp_CS
IPR028601, NUBP1/Nbp35
IPR027417, P-loop_NTPase
IPR033756, YlxH/NBP35
PANTHERiPTHR23264, PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609, ParA, 2 hits
SUPFAMiSSF52540, SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215, MRP, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNBP35_ARATH
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8H1Q2
Secondary accession number(s): Q9C5E1, Q9FI52
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: March 1, 2003
Last modified: June 2, 2021
This is version 110 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again