UniProtKB - Q8H1D3 (NPY1_ARATH)
Protein
BTB/POZ domain-containing protein NPY1
Gene
NPY1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Coregulates with PID the auxin-mediated plant organogenesis. Regulates cotyledon development through control of PIN1 polarity. May play an essential role in root gravitropic responses.By similarity5 Publications
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Biological processi
- apical protein localization Source: TAIR
- basipetal auxin transport Source: TAIR
- cotyledon development Source: TAIR
- flower development Source: TAIR
- inflorescence development Source: TAIR
- positive regulation of flower development Source: TAIR
- protein ubiquitination Source: UniProtKB-UniPathway
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: BTB/POZ domain-containing protein NPY1Alternative name(s): Protein ENHANCER OF PINOID Protein MACCHI-BOU 4 Protein NAKED PINS IN YUC MUTANTS 1 |
Gene namesi | Name:NPY1 Synonyms:ENP, MAB4 Ordered Locus Names:At4g31820 ORF Names:F11C18.20 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G31820 |
TAIRi | locus:2116752, AT4G31820 |
Subcellular locationi
Endosome
- Late endosome 1 Publication
Note: Colocalized with PID.
Endosome
- late endosome Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Keywords - Cellular componenti
EndosomePathology & Biotechi
Disruption phenotypei
Seedlings with abnormal cotyledons.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000409563 | 1 – 571 | BTB/POZ domain-containing protein NPY1Add BLAST | 571 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 409 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8H1D3 |
PRIDEi | Q8H1D3 |
ProteomicsDBi | 250548 |
PTM databases
iPTMneti | Q8H1D3 |
Expressioni
Tissue specificityi
Expressed mainly in the apical regions of embryos including cotyledon tips and the apical meristem. Highly expressed in primary root tips.2 Publications
Developmental stagei
Detected in globular-stage embryos in the epidermis with the highest concentration at the apical region. At early heart stage, expression is restricted to the two cotyledon primordia and the meristem. In the late heart to torpedo stages, expression is restricted to the meristem and the cotyledon tips. In mature embryos, stricly expressed at the apical meristem. During seedling development, highly expressed in young leaf primordia and the apical meristems. Detected in the protodermal cell layer of the embryo and the meristem L1 layer at the site of organ initiation. After transition to the reproductive phase, detected in the inflorescence meristem and at various stages of floral development.2 Publications
Gene expression databases
ExpressionAtlasi | Q8H1D3, baseline and differential |
Genevisiblei | Q8H1D3, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 97 | BTBPROSITE-ProRule annotationAdd BLAST | 69 | |
Domaini | 210 – 468 | NPH3PROSITE-ProRule annotationAdd BLAST | 259 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 540 – 571 | Ser-richAdd BLAST | 32 |
Domaini
The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes.1 Publication
Sequence similaritiesi
Belongs to the NPH3 family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | ENOG502QQX7, Eukaryota |
HOGENOMi | CLU_005994_5_2_1 |
InParanoidi | Q8H1D3 |
OMAi | AYASRWL |
OrthoDBi | 395689at2759 |
PhylomeDBi | Q8H1D3 |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR029961, BTB/POZ_NPY1 IPR043454, NPH3/RPT2-like IPR027356, NPH3_dom IPR011333, SKP1/BTB/POZ_sf |
PANTHERi | PTHR32370, PTHR32370, 1 hit PTHR32370:SF7, PTHR32370:SF7, 1 hit |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF03000, NPH3, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS51649, NPH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8H1D3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFMKLGSKP DTFESDGKFV KYAVSDLDSD VTIHVGEVTF HLHKFPLLSK
60 70 80 90 100
SNRMQRLVFE ASEEKTDEIT ILDMPGGYKA FEICAKFCYG MTVTLNAYNI
110 120 130 140 150
TAVRCAAEYL EMTEDADRGN LIYKIEVFLN SGIFRSWKDS IIVLQTTRSL
160 170 180 190 200
LPWSEDLKLV GRCIDSVSAK ILVNPETITW SYTFNRKLSG PDKIVEYHRE
210 220 230 240 250
KREENVIPKD WWVEDVCELE IDMFKRVISV VKSSGRMNNG VIAEALRYYV
260 270 280 290 300
ARWLPESMES LTSEASSNKD LVETVVFLLP KVNRAMSYSS CSFLLKLLKV
310 320 330 340 350
SILVGADETV REDLVENVSL KLHEASVKDL LIHEVELVHR IVDQFMADEK
360 370 380 390 400
RVSEDDRYKE FVLGNGILLS VGRLIDAYLA LNSELTLSSF VELSELVPES
410 420 430 440 450
ARPIHDGLYK AIDTFMKEHP ELTKSEKKRL CGLMDVRKLT NEASTHAAQN
460 470 480 490 500
ERLPLRVVVQ VLYFEQLRAN HSPVASVAAS SHSPVEKTEE NKGEEATKKV
510 520 530 540 550
ELSKKSRGSK STRSGGGAQL MPSRSRRIFE KIWPGKGEIS NKSSEVSSGS
560 570
SQSPPAKSSS SSSRRRRHSI S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8B383 | A0A1P8B383_ARATH | Phototropic-responsive NPH3 family ... | ENP ENHANCER OF PINOID, MAB4, MACCHI-BOU 4, NAKED PINS IN YUC MUTANTS 1, NPY1 | 518 | Annotation score: |
Sequence cautioni
The sequence CAB40752 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79900 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 226 | R → G in AAM13843 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL049607 Genomic DNA Translation: CAB40752.1 Sequence problems. AL161579 Genomic DNA Translation: CAB79900.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85964.1 AY091021 mRNA Translation: AAM13843.1 AY150514 mRNA Translation: AAN13030.1 |
PIRi | T06304 |
RefSeqi | NP_194910.2, NM_119332.4 |
Genome annotation databases
EnsemblPlantsi | AT4G31820.1; AT4G31820.1; AT4G31820 |
GeneIDi | 829311 |
Gramenei | AT4G31820.1; AT4G31820.1; AT4G31820 |
KEGGi | ath:AT4G31820 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL049607 Genomic DNA Translation: CAB40752.1 Sequence problems. AL161579 Genomic DNA Translation: CAB79900.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85964.1 AY091021 mRNA Translation: AAM13843.1 AY150514 mRNA Translation: AAN13030.1 |
PIRi | T06304 |
RefSeqi | NP_194910.2, NM_119332.4 |
3D structure databases
SMRi | Q8H1D3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT4G31820.1 |
PTM databases
iPTMneti | Q8H1D3 |
Proteomic databases
PaxDbi | Q8H1D3 |
PRIDEi | Q8H1D3 |
ProteomicsDBi | 250548 |
Genome annotation databases
EnsemblPlantsi | AT4G31820.1; AT4G31820.1; AT4G31820 |
GeneIDi | 829311 |
Gramenei | AT4G31820.1; AT4G31820.1; AT4G31820 |
KEGGi | ath:AT4G31820 |
Organism-specific databases
Araporti | AT4G31820 |
TAIRi | locus:2116752, AT4G31820 |
Phylogenomic databases
eggNOGi | ENOG502QQX7, Eukaryota |
HOGENOMi | CLU_005994_5_2_1 |
InParanoidi | Q8H1D3 |
OMAi | AYASRWL |
OrthoDBi | 395689at2759 |
PhylomeDBi | Q8H1D3 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q8H1D3 |
Gene expression databases
ExpressionAtlasi | Q8H1D3, baseline and differential |
Genevisiblei | Q8H1D3, AT |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR029961, BTB/POZ_NPY1 IPR043454, NPH3/RPT2-like IPR027356, NPH3_dom IPR011333, SKP1/BTB/POZ_sf |
PANTHERi | PTHR32370, PTHR32370, 1 hit PTHR32370:SF7, PTHR32370:SF7, 1 hit |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF03000, NPH3, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS51649, NPH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NPY1_ARATH | |
Accessioni | Q8H1D3Primary (citable) accession number: Q8H1D3 Secondary accession number(s): Q8RWY9, Q9SZ49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 28, 2011 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families