UniProtKB - Q8H166 (ALEU_ARATH)
Protein
Thiol protease aleurain
Gene
ALEU
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
May play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation.
Catalytic activityi
- Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase. EC:3.4.22.16
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 165 | By similarity | 1 | |
Active sitei | 305 | By similarity | 1 | |
Active sitei | 325 | By similarity | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- proteolysis involved in cellular protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Protein family/group databases
MEROPSi | I29.003 |
Names & Taxonomyi
Protein namesi | Recommended name: Thiol protease aleurain (EC:3.4.22.16)Short name: AtALEU Alternative name(s): Senescence-associated gene product 2 |
Gene namesi | Name:ALEU Synonyms:AALP, SAG2 Ordered Locus Names:At5g60360 ORF Names:MUF9.1 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G60360 |
Subcellular locationi
Vacuole
- Vacuole 2 Publications
Note: Predominantly vacuolar. From the Golgi apparatus, transported to the lytic vacuole (LV) in clathrin-coated vesicles (CCVs) via the prevacuolar compartment (PVC). In root elongating cells and stomata, localized in central vacuole and in small compartments (LVs, PVCs, Golgi bodies or small vacuoles). Limited to central vacuole in root elongated cells, leaf epidermal cells and trichomes of the lower face of the leaf blade. Limited to small compartments in root cap and apex cells, hypocotyl parenchyma cells and trichomes of the upper face of the leaf blade.
Extracellular region or secreted
- extracellular space Source: GO_Central
Lysosome
- lysosome Source: GO_Central
Vacuole
- lysosome Source: GO_Central
Keywords - Cellular componenti
VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
PropeptideiPRO_0000026414 | 22 – 140 | Activation peptideBy similarityAdd BLAST | 119 | |
ChainiPRO_0000026415 | 141 – 358 | Thiol protease aleurainAdd BLAST | 218 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 125 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 162 ↔ 205 | By similarity | ||
Disulfide bondi | 196 ↔ 238 | By similarity | ||
Glycosylationi | 254 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 296 ↔ 346 | By similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | Q8H166 |
PRIDEi | Q8H166 |
ProteomicsDBi | 244854 [Q8H166-1] |
Expressioni
Tissue specificityi
Expressed in leaves (at protein level).2 Publications
Inductioni
Induced during senescence. Strongly induced by ethylene and slightly by abscisic acid. Repressed by cytokinin and darkness. Seems to be not affected by dehydration.3 Publications
Gene expression databases
ExpressionAtlasi | Q8H166, baseline and differential |
Genevisiblei | Q8H166, AT |
Interactioni
Subunit structurei
Interacts with VSR1/BP80B.
1 PublicationProtein-protein interaction databases
BioGRIDi | 21402, 2 interactors |
IntActi | Q8H166, 1 interactor |
STRINGi | 3702.AT5G60360.3 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 42 | Interaction with VSR11 PublicationAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1543, Eukaryota |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | Q8H166 |
OMAi | AYNNFGC |
PhylomeDBi | Q8H166 |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8H166-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSAKTILSSV VLVVLVAASA AANIGFDESN PIRMVSDGLR EVEESVSQIL
60 70 80 90 100
GQSRHVLSFA RFTHRYGKKY QNVEEMKLRF SIFKENLDLI RSTNKKGLSY
110 120 130 140 150
KLGVNQFADL TWQEFQRTKL GAAQNCSATL KGSHKVTEAA LPETKDWRED
160 170 180 190 200
GIVSPVKDQG GCGSCWTFST TGALEAAYHQ AFGKGISLSE QQLVDCAGAF
210 220 230 240 250
NNYGCNGGLP SQAFEYIKSN GGLDTEKAYP YTGKDETCKF SAENVGVQVL
260 270 280 290 300
NSVNITLGAE DELKHAVGLV RPVSIAFEVI HSFRLYKSGV YTDSHCGSTP
310 320 330 340 350
MDVNHAVLAV GYGVEDGVPY WLIKNSWGAD WGDKGYFKME MGKNMCGIAT
CASYPVVA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8MQZ1 | A8MQZ1_ARATH | Aleurain-like protease | ALP AALP, aleurain-like protease, MUF9.4, MUF9_4, SAG2 | 361 | Annotation score: | ||
F4JYX9 | F4JYX9_ARATH | Aleurain-like protease | ALP AALP, aleurain-like protease, MUF9.4, MUF9_4, SAG2 | 357 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | V → F in AAN31820 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 166 | W → V (PubMed:8518555).Curated | 1 | |
Sequence conflicti | 205 | C → S (PubMed:8518555).Curated | 1 | |
Sequence conflicti | 230 | P → R (PubMed:8518555).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233883 mRNA Translation: AAF43041.1 AF083703 mRNA Translation: AAN60262.1 AB011483 Genomic DNA Translation: BAB08221.1 CP002688 Genomic DNA Translation: AED97313.1 AF360273 mRNA Translation: AAK25983.1 BT000673 mRNA Translation: AAN31819.1 BT000674 mRNA Translation: AAN31820.1 BT000676 mRNA Translation: AAN31822.1 AY088662 mRNA Translation: AAM66984.1 |
PIRi | PQ0650 |
RefSeqi | NP_568921.1, NM_125429.4 [Q8H166-1] |
Genome annotation databases
EnsemblPlantsi | AT5G60360.1; AT5G60360.1; AT5G60360 [Q8H166-1] |
GeneIDi | 836158 |
Gramenei | AT5G60360.1; AT5G60360.1; AT5G60360 [Q8H166-1] |
KEGGi | ath:AT5G60360 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233883 mRNA Translation: AAF43041.1 AF083703 mRNA Translation: AAN60262.1 AB011483 Genomic DNA Translation: BAB08221.1 CP002688 Genomic DNA Translation: AED97313.1 AF360273 mRNA Translation: AAK25983.1 BT000673 mRNA Translation: AAN31819.1 BT000674 mRNA Translation: AAN31820.1 BT000676 mRNA Translation: AAN31822.1 AY088662 mRNA Translation: AAM66984.1 |
PIRi | PQ0650 |
RefSeqi | NP_568921.1, NM_125429.4 [Q8H166-1] |
3D structure databases
SMRi | Q8H166 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 21402, 2 interactors |
IntActi | Q8H166, 1 interactor |
STRINGi | 3702.AT5G60360.3 |
Protein family/group databases
MEROPSi | I29.003 |
Proteomic databases
PaxDbi | Q8H166 |
PRIDEi | Q8H166 |
ProteomicsDBi | 244854 [Q8H166-1] |
Genome annotation databases
EnsemblPlantsi | AT5G60360.1; AT5G60360.1; AT5G60360 [Q8H166-1] |
GeneIDi | 836158 |
Gramenei | AT5G60360.1; AT5G60360.1; AT5G60360 [Q8H166-1] |
KEGGi | ath:AT5G60360 |
Organism-specific databases
Araporti | AT5G60360 |
Phylogenomic databases
eggNOGi | KOG1543, Eukaryota |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | Q8H166 |
OMAi | AYNNFGC |
PhylomeDBi | Q8H166 |
Miscellaneous databases
PROi | PR:Q8H166 |
Gene expression databases
ExpressionAtlasi | Q8H166, baseline and differential |
Genevisiblei | Q8H166, AT |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALEU_ARATH | |
Accessioni | Q8H166Primary (citable) accession number: Q8H166 Secondary accession number(s): Q9LL83, Q9S9A9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | December 2, 2020 | |
This is version 119 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries