Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiol protease aleurain

Gene

ALEU

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation.

Catalytic activityi

Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei165By similarity1
Active sitei305By similarity1
Active sitei325By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

ReactomeiR-ATH-2132295 MHC class II antigen presentation
R-ATH-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiI29.003

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol protease aleurain (EC:3.4.22.16)
Short name:
AtALEU
Alternative name(s):
Senescence-associated gene product 2
Gene namesi
Name:ALEU
Synonyms:AALP, SAG2
Ordered Locus Names:At5g60360
ORF Names:MUF9.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G60360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002641422 – 140Activation peptideBy similarityAdd BLAST119
ChainiPRO_0000026415141 – 358Thiol protease aleurainAdd BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi162 ↔ 205By similarity
Disulfide bondi196 ↔ 238By similarity
Glycosylationi254N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi296 ↔ 346By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ8H166
PRIDEiQ8H166

Expressioni

Tissue specificityi

Expressed in leaves (at protein level).2 Publications

Inductioni

Induced during senescence. Strongly induced by ethylene and slightly by abscisic acid. Repressed by cytokinin and darkness. Seems to be not affected by dehydration.3 Publications

Gene expression databases

ExpressionAtlasiQ8H166 baseline and differential
GenevisibleiQ8H166 AT

Interactioni

Subunit structurei

Interacts with VSR1/BP80B.1 Publication

Protein-protein interaction databases

BioGridi21402, 2 interactors
IntActiQ8H166, 1 interactor
STRINGi3702.AT5G60360.3

Structurei

3D structure databases

ProteinModelPortaliQ8H166
SMRiQ8H166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 42Interaction with VSR11 PublicationAdd BLAST21

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
HOGENOMiHOG000230774
InParanoidiQ8H166
KOiK01366
PhylomeDBiQ8H166

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8H166-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAKTILSSV VLVVLVAASA AANIGFDESN PIRMVSDGLR EVEESVSQIL
60 70 80 90 100
GQSRHVLSFA RFTHRYGKKY QNVEEMKLRF SIFKENLDLI RSTNKKGLSY
110 120 130 140 150
KLGVNQFADL TWQEFQRTKL GAAQNCSATL KGSHKVTEAA LPETKDWRED
160 170 180 190 200
GIVSPVKDQG GCGSCWTFST TGALEAAYHQ AFGKGISLSE QQLVDCAGAF
210 220 230 240 250
NNYGCNGGLP SQAFEYIKSN GGLDTEKAYP YTGKDETCKF SAENVGVQVL
260 270 280 290 300
NSVNITLGAE DELKHAVGLV RPVSIAFEVI HSFRLYKSGV YTDSHCGSTP
310 320 330 340 350
MDVNHAVLAV GYGVEDGVPY WLIKNSWGAD WGDKGYFKME MGKNMCGIAT

CASYPVVA
Length:358
Mass (Da):38,959
Last modified:July 19, 2005 - v2
Checksum:i3B610AB85F81C31D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16V → F in AAN31820 (PubMed:14593172).Curated1
Sequence conflicti166W → V (PubMed:8518555).Curated1
Sequence conflicti205C → S (PubMed:8518555).Curated1
Sequence conflicti230P → R (PubMed:8518555).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233883 mRNA Translation: AAF43041.1
AF083703 mRNA Translation: AAN60262.1
AB011483 Genomic DNA Translation: BAB08221.1
CP002688 Genomic DNA Translation: AED97313.1
AF360273 mRNA Translation: AAK25983.1
BT000673 mRNA Translation: AAN31819.1
BT000674 mRNA Translation: AAN31820.1
BT000676 mRNA Translation: AAN31822.1
AY088662 mRNA Translation: AAM66984.1
PIRiPQ0650
RefSeqiNP_568921.1, NM_125429.4 [Q8H166-1]
UniGeneiAt.25414

Genome annotation databases

EnsemblPlantsiAT5G60360.1; AT5G60360.1; AT5G60360 [Q8H166-1]
GeneIDi836158
GrameneiAT5G60360.1; AT5G60360.1; AT5G60360 [Q8H166-1]
KEGGiath:AT5G60360

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiALEU_ARATH
AccessioniPrimary (citable) accession number: Q8H166
Secondary accession number(s): Q9LL83, Q9S9A9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: June 20, 2018
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health