UniProtKB - Q8H107 (ODO2B_ARATH)
Protein
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial
Gene
At4g26910
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity
Catalytic activityi
- (R)-N6-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N6-(S8-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoAEC:2.3.1.61
Cofactori
(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity
: L-lysine degradation via saccharopine pathway Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes glutaryl-CoA from L-lysine.Proteins known to be involved in the 6 steps of the subpathway in this organism are:
- Lysine ketoglutarate reductase (C24_LOCUS20033), Lysine ketoglutarate reductase (LKR), Lysine ketoglutarate reductase (AXX17_At4g37980), Lysine ketoglutarate reductase (AN1_LOCUS20138), Alpha-aminoadipic semialdehyde synthase (LKR/SDH)
- Lysine ketoglutarate reductase (C24_LOCUS20033), Lysine ketoglutarate reductase (LKR), Lysine ketoglutarate reductase (AXX17_At4g37980), Lysine ketoglutarate reductase (AN1_LOCUS20138), Alpha-aminoadipic semialdehyde synthase (LKR/SDH)
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
- Dihydrolipoyllysine-residue succinyltransferase (C24_LOCUS25606), Dihydrolipoyllysine-residue succinyltransferase (AXX17_At4g31060), Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial (At4g26910), Dihydrolipoyllysine-residue succinyltransferase (AXX17_At5g54170), Dihydrolipoyllysine-residue succinyltransferase (AN1_LOCUS25778), Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial (At5g55070), Dihydrolipoyllysine-residue succinyltransferase (AXX17_At4g31060)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutaryl-CoA from L-lysine, the pathway L-lysine degradation via saccharopine pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 435 | By similarity | 1 | |
Active sitei | 439 | By similarity | 1 |
GO - Molecular functioni
- dihydrolipoyllysine-residue succinyltransferase activity Source: UniProtKB-EC
- zinc ion binding Source: TAIR
GO - Biological processi
- L-lysine catabolic process to acetyl-CoA via saccharopine Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Tricarboxylic acid cycle |
Enzyme and pathway databases
BioCyci | ARA:AT4G26910-MONOMER |
UniPathwayi | UPA00868;UER00840 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial (EC:2.3.1.61)Alternative name(s): 2-oxoglutarate dehydrogenase complex component E2-2 Short name: OGDC-E2-2 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2 E2K-2 |
Gene namesi | Ordered Locus Names:At4g26910 ORF Names:F10M23.250 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G26910 |
TAIRi | locus:2116432, AT4G26910 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication1 Publication
Cytosol
- cytosol Source: TAIR
Mitochondrion
- mitochondrion Source: TAIR
Other locations
- oxoglutarate dehydrogenase complex Source: InterPro
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 85 | Mitochondrion1 PublicationAdd BLAST | 85 | |
ChainiPRO_0000399513 | 86 – 464 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrialAdd BLAST | 379 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 133 | N6-lipoyllysinePROSITE-ProRule annotation | 1 |
Proteomic databases
PaxDbi | Q8H107 |
PRIDEi | Q8H107 |
ProteomicsDBi | 250875 [Q8H107-1] |
Expressioni
Gene expression databases
ExpressionAtlasi | Q8H107, baseline and differential |
Genevisiblei | Q8H107, AT |
Interactioni
Subunit structurei
Forms a 24-polypeptide structural core with octahedral symmetry.
By similarityProtein-protein interaction databases
BioGRIDi | 14085, 5 interactors |
IntActi | Q8H107, 1 interactor |
STRINGi | 3702.AT4G26910.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 92 – 167 | Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST | 76 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 217 – 220 | Poly-Pro | 4 |
Sequence similaritiesi
Belongs to the 2-oxoacid dehydrogenase family.Curated
Keywords - Domaini
Lipoyl, Transit peptidePhylogenomic databases
eggNOGi | KOG0559, Eukaryota |
InParanoidi | Q8H107 |
OMAi | DHISALQ |
OrthoDBi | 850255at2759 |
PhylomeDBi | Q8H107 |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR011053, Single_hybrid_motif IPR006255, SucB |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR01347, sucB, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8H107-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMMRAVIRRA ASNGSSPSLF AKSLQSSRVA ASSPSLLSGS ETGAYLHRGN
60 70 80 90 100
HAHSFHNLAL PAGNSGISRS ASLVSSTLQR WVRPFSAETG DTVEAVVPHM
110 120 130 140 150
GESITDGTLA TFLKKPGERV QADEAIAQIE TDKVTIDIAS PASGVIQEFL
160 170 180 190 200
VNEGDTVEPG TKVAIISKSE DTASQVTPSQ KIPETTDTKP SPPAEDKQKP
210 220 230 240 250
RVESAPVAEK PKAPSSPPPP KQSAKEPQLP PKERERRVPM TRLRKRVATR
260 270 280 290 300
LKDSQNTFAL LTTFNEVDMT NLMKLRSQYK DAFYEKHGVK LGLMSGFIKA
310 320 330 340 350
AVSALQHQPV VNAVIDGDDI IYRDYVDISI AVGTSKGLVV PVIRGADKMN
360 370 380 390 400
FAEIEKTINS LAKKANEGTI SIDEMAGGSF TVSNGGVYGS LISTPIINPP
410 420 430 440 450
QSAILGMHSI VSRPMVVGGS VVPRPMMYVA LTYDHRLIDG REAVYFLRRV
460
KDVVEDPQRL LLDI
Sequence cautioni
The sequence AAM67267 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AY096643 differs from that shown. Reason: Frameshift.Curated
The sequence CAB36537 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79546 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | M → L in AAM67267 (Ref. 5) Curated | 1 | |
Sequence conflicti | 21 | A → G in AAM67267 (Ref. 5) Curated | 1 | |
Sequence conflicti | 201 | R → K in AAM67267 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039869 | 1 – 99 | Missing in isoform 3. 1 PublicationAdd BLAST | 99 | |
Alternative sequenceiVSP_039870 | 62 | Missing in isoform 2. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL035440 Genomic DNA Translation: CAB36537.1 Sequence problems. AL161566 Genomic DNA Translation: CAB79546.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85267.1 CP002687 Genomic DNA Translation: AEE85268.1 CP002687 Genomic DNA Translation: AEE85269.1 AY096643 mRNA No translation available. BT000926 mRNA Translation: AAN41326.1 AK317635 mRNA Translation: BAH20297.1 AY084248 mRNA Translation: AAM67267.1 Different initiation. |
PIRi | T04814 |
RefSeqi | NP_567761.1, NM_118825.3 [Q8H107-1] NP_849452.1, NM_179121.3 [Q8H107-2] NP_849453.1, NM_179122.1 [Q8H107-3] |
Genome annotation databases
EnsemblPlantsi | AT4G26910.1; AT4G26910.1; AT4G26910 [Q8H107-1] AT4G26910.2; AT4G26910.2; AT4G26910 [Q8H107-2] AT4G26910.3; AT4G26910.3; AT4G26910 [Q8H107-3] |
GeneIDi | 828798 |
Gramenei | AT4G26910.1; AT4G26910.1; AT4G26910 [Q8H107-1] AT4G26910.2; AT4G26910.2; AT4G26910 [Q8H107-2] AT4G26910.3; AT4G26910.3; AT4G26910 [Q8H107-3] |
KEGGi | ath:AT4G26910 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL035440 Genomic DNA Translation: CAB36537.1 Sequence problems. AL161566 Genomic DNA Translation: CAB79546.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85267.1 CP002687 Genomic DNA Translation: AEE85268.1 CP002687 Genomic DNA Translation: AEE85269.1 AY096643 mRNA No translation available. BT000926 mRNA Translation: AAN41326.1 AK317635 mRNA Translation: BAH20297.1 AY084248 mRNA Translation: AAM67267.1 Different initiation. |
PIRi | T04814 |
RefSeqi | NP_567761.1, NM_118825.3 [Q8H107-1] NP_849452.1, NM_179121.3 [Q8H107-2] NP_849453.1, NM_179122.1 [Q8H107-3] |
3D structure databases
SMRi | Q8H107 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 14085, 5 interactors |
IntActi | Q8H107, 1 interactor |
STRINGi | 3702.AT4G26910.1 |
Proteomic databases
PaxDbi | Q8H107 |
PRIDEi | Q8H107 |
ProteomicsDBi | 250875 [Q8H107-1] |
Genome annotation databases
EnsemblPlantsi | AT4G26910.1; AT4G26910.1; AT4G26910 [Q8H107-1] AT4G26910.2; AT4G26910.2; AT4G26910 [Q8H107-2] AT4G26910.3; AT4G26910.3; AT4G26910 [Q8H107-3] |
GeneIDi | 828798 |
Gramenei | AT4G26910.1; AT4G26910.1; AT4G26910 [Q8H107-1] AT4G26910.2; AT4G26910.2; AT4G26910 [Q8H107-2] AT4G26910.3; AT4G26910.3; AT4G26910 [Q8H107-3] |
KEGGi | ath:AT4G26910 |
Organism-specific databases
Araporti | AT4G26910 |
TAIRi | locus:2116432, AT4G26910 |
Phylogenomic databases
eggNOGi | KOG0559, Eukaryota |
InParanoidi | Q8H107 |
OMAi | DHISALQ |
OrthoDBi | 850255at2759 |
PhylomeDBi | Q8H107 |
Enzyme and pathway databases
UniPathwayi | UPA00868;UER00840 |
BioCyci | ARA:AT4G26910-MONOMER |
Miscellaneous databases
PROi | PR:Q8H107 |
Gene expression databases
ExpressionAtlasi | Q8H107, baseline and differential |
Genevisiblei | Q8H107, AT |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR011053, Single_hybrid_motif IPR006255, SucB |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR01347, sucB, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ODO2B_ARATH | |
Accessioni | Q8H107Primary (citable) accession number: Q8H107 Secondary accession number(s): Q3E9W2, Q8LGI7, Q9SZ31 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | October 5, 2010 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families