UniProtKB - Q8H0U4 (TNPO1_ARATH)
Protein
Transportin-1
Gene
TRN1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the glycine-rich RNA-binding RBG7 and mediates its nuclear import.By similarity1 Publication
GO - Molecular functioni
- nuclear import signal receptor activity Source: GO_Central
- nuclear localization sequence binding Source: GO_Central
- small GTPase binding Source: InterPro
GO - Biological processi
- import into nucleus Source: TAIR
- miRNA loading onto RISC involved in gene silencing by miRNA Source: TAIR
- protein import into nucleus Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Transportin-1Short name: AtTRN1 Alternative name(s): Importin beta-2 Karyopherin beta-2 |
Gene namesi | Name:TRN1 Ordered Locus Names:At2g16950 ORF Names:F12A24.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G16950 |
TAIRi | locus:2039410, AT2G16950 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- nucleoplasm 1 Publication
Note: Shuttles continuously between the nucleus and the cytoplasm.
Cytosol
- cytosol Source: TAIR
Nucleus
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: TAIR
Other locations
- cytoplasm Source: TAIR
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000421800 | 2 – 891 | Transportin-1Add BLAST | 890 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q8H0U4 |
PRIDEi | Q8H0U4 |
ProMEXi | Q8H0U4 |
ProteomicsDBi | 232467 [Q8H0U4-1] |
PTM databases
iPTMneti | Q8H0U4 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q8H0U4, baseline and differential |
Genevisiblei | Q8H0U4, AT |
Interactioni
Subunit structurei
Interacts with RBG7, RBG8, RAN1 and RNP1.
1 PublicationGO - Molecular functioni
- small GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 1555, 4 interactors |
IntActi | Q8H0U4, 6 interactors |
STRINGi | 3702.AT2G16950.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 14 – 42 | HEAT 1By similarityAdd BLAST | 29 | |
Domaini | 37 – 105 | Importin N-terminalPROSITE-ProRule annotationAdd BLAST | 69 | |
Repeati | 47 – 85 | HEAT 2By similarityAdd BLAST | 39 | |
Repeati | 94 – 127 | HEAT 3By similarityAdd BLAST | 34 | |
Repeati | 133 – 170 | HEAT 4By similarityAdd BLAST | 38 | |
Repeati | 180 – 210 | HEAT 5By similarityAdd BLAST | 31 | |
Repeati | 223 – 250 | HEAT 6By similarityAdd BLAST | 28 | |
Repeati | 262 – 289 | HEAT 7By similarityAdd BLAST | 28 | |
Repeati | 305 – 382 | HEAT 8By similarityAdd BLAST | 78 | |
Repeati | 390 – 421 | HEAT 9By similarityAdd BLAST | 32 | |
Repeati | 433 – 460 | HEAT 10By similarityAdd BLAST | 28 | |
Repeati | 478 – 511 | HEAT 11By similarityAdd BLAST | 34 | |
Repeati | 519 – 552 | HEAT 12By similarityAdd BLAST | 34 | |
Repeati | 560 – 598 | HEAT 13By similarityAdd BLAST | 39 | |
Repeati | 606 – 654 | HEAT 14By similarityAdd BLAST | 49 | |
Repeati | 665 – 696 | HEAT 15By similarityAdd BLAST | 32 | |
Repeati | 704 – 741 | HEAT 16By similarityAdd BLAST | 38 | |
Repeati | 749 – 785 | HEAT 17By similarityAdd BLAST | 37 | |
Repeati | 793 – 826 | HEAT 18By similarityAdd BLAST | 34 | |
Repeati | 835 – 867 | HEAT 19By similarityAdd BLAST | 33 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 319 – 361 | Asp-richAdd BLAST | 43 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2023, Eukaryota |
HOGENOMi | CLU_008136_1_0_1 |
OMAi | INVFTPR |
OrthoDBi | 525700at2759 |
PhylomeDBi | Q8H0U4 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N IPR040122, Importin_beta |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8H0U4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAATAVVWQP RDDGLAEICS LLEQQISPSS VVDKSQIWKQ LQHFSQFPDF
60 70 80 90 100
NNYLVFILVR AEGKSVEVRQ AAGLLLKNNL RGAYPSMTQE NQKYIKSELL
110 120 130 140 150
PCLGAADRNI RTTVGTIISV IVNIEGVSGW HELLPALVTC LDSNDLNHMD
160 170 180 190 200
GAMDALSKIC EDIPHVLDTE VPGLAERPIN IFLPRLLQFF QSPHASLRKL
210 220 230 240 250
ALGSVNQYII IMPAALYNSL DKYLQGLFVL ANDPVPEVRK LVCAAFVHLT
260 270 280 290 300
EVLPSSIEPH LRNVMEYMLQ VNRDPDEEVS LEACEFWSAY CDAQLPPENL
310 320 330 340 350
KEFLPRLIPV LLENMAYADD DESLLDAEED ESQPDRDQDL KPRFHTSRLH
360 370 380 390 400
GSEDFDDDDD DSFNVWNLRK CSAAAIDVLS NVFGDEILPA LMPLIQKNLS
410 420 430 440 450
ASGDEAWKQR EAAVLALGAI AEGCMNGLYP HLSEIVAFLL PLLDDKFPLI
460 470 480 490 500
RSISCWTLSR FGKYLIQESG NPKGYEQFEK VLMGLLRRLL DTNKRVQEAA
510 520 530 540 550
CSAFATVEED AAEELVPHLG VILQHLMCAF GKYQRRNLRI VYDAIGTLAD
560 570 580 590 600
SVREELNKPA YLEILMPPLV AKWQQLSNSD KDLFPLLECF TSISQALGVG
610 620 630 640 650
FAPFAQPVFQ RCMDIIQLQQ LAKVNPASAG AQYDREFIVC SLDLLSGLAE
660 670 680 690 700
GLGSGIESLV QQSNLRDLLL NCCIDEAADV RQSAFALMGD LARVFPVYLQ
710 720 730 740 750
PRLLDFLEIA SQQLSANLNR ENLSVANNAC WAIGELAVKV RQEVSPIVAK
760 770 780 790 800
VVSSLGLILQ HGEGVNKALV ENSAITLGRL AWIRPDLVAP HMDHFMKPWC
810 820 830 840 850
MALSMVRDDI EKEDAFRGLC AVVKVNPSGG VSSLVFICQA IASWHEIRSE
860 870 880 890
DVQTEVSQVL NGYKHMLGNS WAECLSALDP PVKERLARYQ V
Sequence cautioni
The sequence AAC64225 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Isoform 2 (identifier: Q8H0U4-2) | |||||
Sequence conflicti | 216 – 217 | IW → R in CAC80068 (PubMed:14756317).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045998 | 214 | A → AVIWQ in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y18342 mRNA Translation: CAC80068.1 AC005167 Genomic DNA Translation: AAC64225.1 Sequence problems. CP002685 Genomic DNA Translation: AEC06561.1 CP002685 Genomic DNA Translation: AEC06562.1 BT002041 mRNA Translation: AAN72052.1 BT008804 mRNA Translation: AAP68243.1 |
PIRi | C84546 |
RefSeqi | NP_001031359.1, NM_001036282.2 [Q8H0U4-1] NP_179287.3, NM_127248.4 [Q8H0U4-2] |
Genome annotation databases
EnsemblPlantsi | AT2G16950.1; AT2G16950.1; AT2G16950 [Q8H0U4-2] AT2G16950.2; AT2G16950.2; AT2G16950 [Q8H0U4-1] |
GeneIDi | 816198 |
Gramenei | AT2G16950.1; AT2G16950.1; AT2G16950 [Q8H0U4-2] AT2G16950.2; AT2G16950.2; AT2G16950 [Q8H0U4-1] |
KEGGi | ath:AT2G16950 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y18342 mRNA Translation: CAC80068.1 AC005167 Genomic DNA Translation: AAC64225.1 Sequence problems. CP002685 Genomic DNA Translation: AEC06561.1 CP002685 Genomic DNA Translation: AEC06562.1 BT002041 mRNA Translation: AAN72052.1 BT008804 mRNA Translation: AAP68243.1 |
PIRi | C84546 |
RefSeqi | NP_001031359.1, NM_001036282.2 [Q8H0U4-1] NP_179287.3, NM_127248.4 [Q8H0U4-2] |
3D structure databases
SMRi | Q8H0U4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1555, 4 interactors |
IntActi | Q8H0U4, 6 interactors |
STRINGi | 3702.AT2G16950.1 |
PTM databases
iPTMneti | Q8H0U4 |
Proteomic databases
PaxDbi | Q8H0U4 |
PRIDEi | Q8H0U4 |
ProMEXi | Q8H0U4 |
ProteomicsDBi | 232467 [Q8H0U4-1] |
Genome annotation databases
EnsemblPlantsi | AT2G16950.1; AT2G16950.1; AT2G16950 [Q8H0U4-2] AT2G16950.2; AT2G16950.2; AT2G16950 [Q8H0U4-1] |
GeneIDi | 816198 |
Gramenei | AT2G16950.1; AT2G16950.1; AT2G16950 [Q8H0U4-2] AT2G16950.2; AT2G16950.2; AT2G16950 [Q8H0U4-1] |
KEGGi | ath:AT2G16950 |
Organism-specific databases
Araporti | AT2G16950 |
TAIRi | locus:2039410, AT2G16950 |
Phylogenomic databases
eggNOGi | KOG2023, Eukaryota |
HOGENOMi | CLU_008136_1_0_1 |
OMAi | INVFTPR |
OrthoDBi | 525700at2759 |
PhylomeDBi | Q8H0U4 |
Miscellaneous databases
PROi | PR:Q8H0U4 |
Gene expression databases
ExpressionAtlasi | Q8H0U4, baseline and differential |
Genevisiblei | Q8H0U4, AT |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N IPR040122, Importin_beta |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TNPO1_ARATH | |
Accessioni | Q8H0U4Primary (citable) accession number: Q8H0U4 Secondary accession number(s): F4ILE2, Q8H2D6, Q9ZVW8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 6, 2013 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families