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Protein

Polyribonucleotide nucleotidyltransferase 1, chloroplastic

Gene

PNP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the metabolism of all major classes of plastid RNAs. Required for efficient 3'-end processing of mRNAs and 3'-end maturation of rRNA transcripts, but is not sufficient to mediate their degradation. Mediates tRNA degradation. May function as a poly(A) mRNA 3'-5' degrading phosphorylase. May be required for plastid ribosome assembly and non-coding RNA biogenesis and accumulation. Seems not required for efficient translation.4 Publications

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.

GO - Molecular functioni

  • 3'-5'-exoribonuclease activity Source: UniProtKB
  • polyribonucleotide nucleotidyltransferase activity Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, RNA-binding, Transferase
Biological processmRNA processing, rRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase 1, chloroplastic (EC:2.7.7.8)
Short name:
AtcpPNPase
Alternative name(s):
Polynucleotide phosphorylase 1
Short name:
PNPase 1
Protein PIGMENT DEFECTIVE 326
Protein RESISTANT TO INHIBITION WITH FSM 10
Gene namesi
Name:PNP1
Synonyms:PDE326, RIF10
Ordered Locus Names:At3g03710
ORF Names:F20H23.26, T12J13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G03710
TAIRilocus:2079429 AT3G03710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Delayed greening and retarded growth.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176R → S: Reduces RNA-binding affinity; when associated with L-184. 1 Publication1
Mutagenesisi184P → L: Reduces RNA-binding affinity; when associated with S-176. 1 Publication1
Mutagenesisi202S → N: No effect on activity or DNA binding. 1 Publication1
Mutagenesisi596G → R: Loss of activity. 1 Publication1
Mutagenesisi625D → N: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
ChainiPRO_000042027549 – 922Polyribonucleotide nucleotidyltransferase 1, chloroplasticAdd BLAST874

Proteomic databases

PaxDbiQ8GZQ3
PRIDEiQ8GZQ3

PTM databases

iPTMnetiQ8GZQ3

Expressioni

Gene expression databases

ExpressionAtlasiQ8GZQ3 baseline and differential
GenevisibleiQ8GZQ3 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G03710.1

Structurei

3D structure databases

ProteinModelPortaliQ8GZQ3
SMRiQ8GZQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini692 – 752KHAdd BLAST61
Domaini762 – 831S1 motifAdd BLAST70

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1067 Eukaryota
COG1185 LUCA
HOGENOMiHOG000218327
InParanoidiQ8GZQ3
KOiK00962
OMAiGGKLCSL
OrthoDBiEOG0936088V
PhylomeDBiQ8GZQ3

Family and domain databases

Gene3Di3.30.230.70, 1 hit
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 1 hit
PF00575 S1, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GZQ3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLTSPSNALH SSTPQFWPLR RSKLCRSRNF PRFHSGERSS GGGGKLCSLS
60 70 80 90 100
LLSGSGAGKF SVRALVRPDD TDDADSVGDG SLAFPNHVSV KIPFGNREIL
110 120 130 140 150
VETGLMGRQA SSAVTVTDGE TIVYTSVCLA DVPSEPSDFL PLYVHYQERF
160 170 180 190 200
SAVGRTSGGF FKREGRTKDH EVLICRLIDR PLRPTMPKGF YNETQILSWV
210 220 230 240 250
LSYDGLHAPD ALAVTSAGIA VALSEVPNAK AIAGVRVGLI GGEFIVNPTV
260 270 280 290 300
KEMEESQLDL FLAGTDTAIL TIEGYSNFLP EEMLLQAVKV GQDAVQATCI
310 320 330 340 350
AIEVLAKKYG KPKMLDAIRL PPPELYKHVK ELAGEELTKA LQIKSKISRR
360 370 380 390 400
KAISSLEEKV LTILTEKGYV IDEVAFGTIE AQPDLLEDED EDEEVVPEGE
410 420 430 440 450
VDQGDVHIRP IPRKPIPLLF SEVDVKLVFK EVSSKLLRRR IVEGGKRSDG
460 470 480 490 500
RTLDEIRPIN SRCGLLPRAH GSTLFTRGET QALAVVTLGD KQMAQRIDNL
510 520 530 540 550
EGSDEYKRFY LQYTFPPSSV GEVGRIGAPS RREIGHGTLA ERALETILPS
560 570 580 590 600
DDDFPYTIRV ESTVIESNGS SSMASVCGGC LALQDAGVPV KCSVAGIAMG
610 620 630 640 650
MVWDTEEFGG DGSPLILSDI TGAEDASGDM DFKVAGNEDG VTAFQMDIKV
660 670 680 690 700
GGITLEIMEK ALIQAKAGRR HILAEMAKCS PPPTLSLSKY APLILIMKVH
710 720 730 740 750
PSKVYSLIGS GGKKVKSIIE ESGVEAIDMQ DDGTVKIMAI DVASLERAKA
760 770 780 790 800
IISGLTMVPS VGDIYRNCEI KSMAPYGAFV EIAPGREGLC HISELSAEWL
810 820 830 840 850
AKPEDAYKVG DRIDVKLIEV NEKGQLRLSV RALLPESETD KDSQKQQPAG
860 870 880 890 900
DSTKDKSSQR KYVNTSSKDR AAAGASKVSS GDELVLKKKD VRRATGGSSD
910 920
KTMNSNSSTN EESLVNGEAT IS
Length:922
Mass (Da):99,566
Last modified:March 1, 2003 - v1
Checksum:iA6FFBB738F762838
GO

Sequence cautioni

The sequence AAF00646 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF03462 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti355S → P in CAB85703 (Ref. 2) Curated1
Sequence conflicti823K → E in CAB85703 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ252123 mRNA Translation: CAB85703.1
AF450480 mRNA Translation: AAN76771.1
AC009327 Genomic DNA Translation: AAF03462.1 Sequence problems.
AC009540 Genomic DNA Translation: AAF00646.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE73974.1
RefSeqiNP_187021.2, NM_111242.3
UniGeneiAt.26536

Genome annotation databases

EnsemblPlantsiAT3G03710.1; AT3G03710.1; AT3G03710
GeneIDi821181
GrameneiAT3G03710.1; AT3G03710.1; AT3G03710
KEGGiath:AT3G03710

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ252123 mRNA Translation: CAB85703.1
AF450480 mRNA Translation: AAN76771.1
AC009327 Genomic DNA Translation: AAF03462.1 Sequence problems.
AC009540 Genomic DNA Translation: AAF00646.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE73974.1
RefSeqiNP_187021.2, NM_111242.3
UniGeneiAt.26536

3D structure databases

ProteinModelPortaliQ8GZQ3
SMRiQ8GZQ3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G03710.1

PTM databases

iPTMnetiQ8GZQ3

Proteomic databases

PaxDbiQ8GZQ3
PRIDEiQ8GZQ3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G03710.1; AT3G03710.1; AT3G03710
GeneIDi821181
GrameneiAT3G03710.1; AT3G03710.1; AT3G03710
KEGGiath:AT3G03710

Organism-specific databases

AraportiAT3G03710
TAIRilocus:2079429 AT3G03710

Phylogenomic databases

eggNOGiKOG1067 Eukaryota
COG1185 LUCA
HOGENOMiHOG000218327
InParanoidiQ8GZQ3
KOiK00962
OMAiGGKLCSL
OrthoDBiEOG0936088V
PhylomeDBiQ8GZQ3

Miscellaneous databases

PROiPR:Q8GZQ3

Gene expression databases

ExpressionAtlasiQ8GZQ3 baseline and differential
GenevisibleiQ8GZQ3 AT

Family and domain databases

Gene3Di3.30.230.70, 1 hit
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 1 hit
PF00575 S1, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPNP1_ARATH
AccessioniPrimary (citable) accession number: Q8GZQ3
Secondary accession number(s): Q9M497, Q9S749
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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