UniProtKB - Q8GZM7 (PIF1_ARATH)
Protein
Transcription factor PIF1
Gene
PIF1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Transcription activator. Regulates negatively chlorophyll biosynthesis and seed germination in the dark, and lightinduced degradation of PIF1 relieves this negative regulation to promote photomorphogenesis. Binds to the G-box motif (5'-CACGTG-3') found in many light-regulated promoters. Promotes the expression of SOM, and thus modulates responses to abscisic acid (ABA) and gibberellic acid (GA).5 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 47 | Pfr PHYB binding | 1 | |
Sitei | 95 | Pfr PHYA binding | 1 | |
Sitei | 144 | Pfr PHYA binding | 1 |
GO - Molecular functioni
- DNA binding Source: TAIR
- DNA-binding transcription factor activity Source: TAIR
- identical protein binding Source: IntAct
- phytochrome binding Source: TAIR
- protein dimerization activity Source: InterPro
GO - Biological processi
- chlorophyll biosynthetic process Source: TAIR
- gibberellic acid mediated signaling pathway Source: TAIR
- heme biosynthetic process Source: TAIR
- negative gravitropism Source: TAIR
- negative regulation of photomorphogenesis Source: TAIR
- negative regulation of seed germination Source: TAIR
- red light signaling pathway Source: TAIR
- regulation of photomorphogenesis Source: TAIR
- regulation of seed germination Source: TAIR
- regulation of transcription, DNA-templated Source: TAIR
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor PIF1Alternative name(s): Basic helix-loop-helix protein 15 Short name: AtbHLH15 Short name: bHLH 15 Protein PHY-INTERACTING FACTOR 1 Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 Transcription factor EN 101 bHLH transcription factor bHLH015 |
Gene namesi | Name:PIF1 Synonyms:BHLH15, EN101, PIL5 Ordered Locus Names:At2g20180 ORF Names:T2G17.2 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G20180 |
TAIRi | locus:2061634, AT2G20180 |
Subcellular locationi
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Plants overaccumulate free protochlorophyllide in the darkness and exhibit photooxidative damage (bleaching) in subsequent light, probably caused by the photosensitizing activity of this tetrapyrrole intermediate.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 50 | Missing : Normal interaction with PHYA (Pfr form). 1 PublicationAdd BLAST | 50 | |
Mutagenesisi | 10 | T → A: Loss of phosphorylation by CK2. 1 Publication | 1 | |
Mutagenesisi | 41 | E → A: Loss of interaction with PHYB (Pfr form). 1 Publication | 1 | |
Mutagenesisi | 42 | L → A: Loss of interaction with PHYB (Pfr form). 1 Publication | 1 | |
Mutagenesisi | 44 | W → A: Reduced interaction with PHYB (Pfr form). 1 Publication | 1 | |
Mutagenesisi | 47 | G → A: Loss of interaction with PHYB (Pfr form). 1 Publication | 1 | |
Mutagenesisi | 85 – 95 | Missing : Reduced interaction with PHYA (Pfr form). 1 PublicationAdd BLAST | 11 | |
Mutagenesisi | 95 | L → A: Reduced interaction with PHYA (Pfr form). Reduced interaction with PHYA (Pfr form); when associated with A-123; A-153; or A-160. Loss of interaction with PHYA (Pfr form); when associated with A-144. 1 Publication | 1 | |
Mutagenesisi | 118 – 160 | Missing : Reduced interaction with PHYA (Pfr form). 1 PublicationAdd BLAST | 43 | |
Mutagenesisi | 123 | S → A: Reduced interaction with PHYA (Pfr form); when associated with A-95. 1 Publication | 1 | |
Mutagenesisi | 144 | N → A: Loss of interaction with PHYA (Pfr form); when associated with A-95. 1 Publication | 1 | |
Mutagenesisi | 148 | F → A: Normal interaction with PHYA (Pfr form). 1 Publication | 1 | |
Mutagenesisi | 153 | G → A: Reduced interaction with PHYA (Pfr form); when associated with A-95. 1 Publication | 1 | |
Mutagenesisi | 155 | F → A: Normal interaction with PHYA (Pfr form). 1 Publication | 1 | |
Mutagenesisi | 160 | G → A: Reduced interaction with PHYA (Pfr form); when associated with A-95. 1 Publication | 1 | |
Mutagenesisi | 197 | T → A: Loss of phosphorylation by CK2. 1 Publication | 1 | |
Mutagenesisi | 202 | S → A: Loss of phosphorylation by CK2. 1 Publication | 1 | |
Mutagenesisi | 464 | S → A: Loss of phosphorylation by CK2. 1 Publication | 1 | |
Mutagenesisi | 465 | S → A: Loss of phosphorylation by CK2. 1 Publication | 1 | |
Mutagenesisi | 466 | S → A: Loss of phosphorylation by CK2. 1 Publication | 1 | |
Mutagenesisi | 469 | S → A: Loss of phosphorylation by CK2. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000358829 | 1 – 478 | Transcription factor PIF1Add BLAST | 478 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | Phosphothreonine; by CK21 Publication | 1 | |
Modified residuei | 197 | Phosphothreonine; by CK21 Publication | 1 | |
Modified residuei | 202 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 464 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 465 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 466 | Phosphoserine; by CK21 Publication | 1 | |
Modified residuei | 469 | Phosphoserine; by CK21 Publication | 1 |
Post-translational modificationi
Phosphorylated at Thr-10, Thr-197, Ser-202, Ser-464, Ser-465, Ser-466 and Ser-469 by CK2 (PubMed:21330376). Phosphorylated and ubiquitinated after an exposure to light (especially red and far-red), in a phytochrome-dependent manner. Modified proteins undergo a proteasome-dependent degradation. Its stability and degradation plays a central role in photomorphogenesis of seedlings.3 Publications
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q8GZM7 |
ProteomicsDBi | 235014 [Q8GZM7-1] |
PTM databases
iPTMneti | Q8GZM7 |
Expressioni
Tissue specificityi
Expressed constitutively in roots, leaves, stems, and flowers.1 Publication
Inductioni
Repressed by red (R) and far red (FR) light treatments in a phyB- and phyA-dependent manner.1 Publication
Gene expression databases
ExpressionAtlasi | Q8GZM7, baseline and differential |
Genevisiblei | Q8GZM7, AT |
Interactioni
Subunit structurei
Homodimer (Probable).
Interacts with the photoactivated conformer (Pfr) of phytochromes A and B, PHYA and PHYB.
Interacts also with APRR1/TOC1. Binds to RGL2, RGA and FHY3 (via N-terminus).
Curated5 PublicationsBinary interactionsi
Hide detailsQ8GZM7
With | #Exp. | IntAct |
---|---|---|
BZR1 [Q8S307] | 2 | EBI-630400,EBI-1803261 |
HFR1 [Q9FE22] | 3 | EBI-630400,EBI-626001 |
PHYB [P14713] | 3 | EBI-630400,EBI-300727 |
itself | 3 | EBI-630400,EBI-630400 |
PIF3 [O80536] | 6 | EBI-630400,EBI-625701 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- phytochrome binding Source: TAIR
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 1892, 24 interactors |
DIPi | DIP-61853N |
IntActi | Q8GZM7, 10 interactors |
STRINGi | 3702.AT2G20180.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 284 – 333 | bHLHPROSITE-ProRule annotationAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 339 – 382 | Met-richAdd BLAST | 44 |
Phylogenomic databases
eggNOGi | ENOG502QR6A, Eukaryota |
InParanoidi | Q8GZM7 |
OMAi | AQMGPRF |
OrthoDBi | 1384644at2759 |
PhylomeDBi | Q8GZM7 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR031066, bHLH_ALC-like_plant IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR45855, PTHR45855, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8GZM7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHHFVPDFDT DDDYVNNHNS SLNHLPRKSI TTMGEDDDLM ELLWQNGQVV
60 70 80 90 100
VQNQRLHTKK PSSSPPKLLP SMDPQQQPSS DQNLFIQEDE MTSWLHYPLR
110 120 130 140 150
DDDFCSDLLF SAAPTATATA TVSQVTAARP PVSSTNESRP PVRNFMNFSR
160 170 180 190 200
LRGDFNNGRG GESGPLLSKA VVRESTQVSP SATPSAAASE SGLTRRTDGT
210 220 230 240 250
DSSAVAGGGA YNRKGKAVAM TAPAIEITGT SSSVVSKSEI EPEKTNVDDR
260 270 280 290 300
KRKEREATTT DETESRSEET KQARVSTTST KRSRAAEVHN LSERKRRDRI
310 320 330 340 350
NERMKALQEL IPRCNKSDKA SMLDEAIEYM KSLQLQIQMM SMGCGMMPMM
360 370 380 390 400
YPGMQQYMPH MAMGMGMNQP IPPPSFMPFP NMLAAQRPLP TQTHMAGSGP
410 420 430 440 450
QYPVHASDPS RVFVPNQQYD PTSGQPQYPA GYTDPYQQFR GLHPTQPPQF
460 470
QNQATSYPSS SRVSSSKESE DHGNHTTG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AYE4 | A0A1P8AYE4_ARATH | Phytochrome interacting factor 3-li... | PIL5 PHY-INTERACTING FACTOR 1, PIF1, At2g20180, T2G17.2, T2G17_2 | 342 | Annotation score: |
Sequence cautioni
The sequence AAD24380 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | D → G in BAF00716 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036107 | 1 – 71 | Missing in isoform 2. 2 PublicationsAdd BLAST | 71 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF488560 mRNA Translation: AAN78308.1 AB103113 Transcribed RNA Translation: BAC56979.1 AC006081 Genomic DNA Translation: AAD24380.1 Sequence problems. CP002685 Genomic DNA Translation: AEC06977.1 CP002685 Genomic DNA Translation: AEC06978.1 CP002685 Genomic DNA Translation: AEC06979.1 CP002685 Genomic DNA Translation: ANM61700.1 CP002685 Genomic DNA Translation: ANM61701.1 CP002685 Genomic DNA Translation: ANM61702.1 CP002685 Genomic DNA Translation: ANM61703.1 AK228820 mRNA Translation: BAF00716.1 BT029775 mRNA Translation: ABM06045.1 |
PIRi | A84586 |
RefSeqi | NP_001189559.1, NM_001202630.2 [Q8GZM7-1] NP_001318252.1, NM_001335663.1 [Q8GZM7-1] NP_001323902.1, NM_001335664.1 [Q8GZM7-1] NP_001323903.1, NM_001335665.1 [Q8GZM7-2] NP_001323904.1, NM_001335666.1 [Q8GZM7-1] NP_001323905.1, NM_001335667.1 [Q8GZM7-2] NP_849996.1, NM_179665.3 [Q8GZM7-2] |
Genome annotation databases
EnsemblPlantsi | AT2G20180.1; AT2G20180.1; AT2G20180 [Q8GZM7-2] AT2G20180.2; AT2G20180.2; AT2G20180 [Q8GZM7-1] AT2G20180.3; AT2G20180.3; AT2G20180 [Q8GZM7-1] AT2G20180.4; AT2G20180.4; AT2G20180 [Q8GZM7-1] AT2G20180.5; AT2G20180.5; AT2G20180 [Q8GZM7-2] AT2G20180.6; AT2G20180.6; AT2G20180 [Q8GZM7-1] AT2G20180.7; AT2G20180.7; AT2G20180 [Q8GZM7-2] |
GeneIDi | 816538 |
Gramenei | AT2G20180.1; AT2G20180.1; AT2G20180 [Q8GZM7-2] AT2G20180.2; AT2G20180.2; AT2G20180 [Q8GZM7-1] AT2G20180.3; AT2G20180.3; AT2G20180 [Q8GZM7-1] AT2G20180.4; AT2G20180.4; AT2G20180 [Q8GZM7-1] AT2G20180.5; AT2G20180.5; AT2G20180 [Q8GZM7-2] AT2G20180.6; AT2G20180.6; AT2G20180 [Q8GZM7-1] AT2G20180.7; AT2G20180.7; AT2G20180 [Q8GZM7-2] |
KEGGi | ath:AT2G20180 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF488560 mRNA Translation: AAN78308.1 AB103113 Transcribed RNA Translation: BAC56979.1 AC006081 Genomic DNA Translation: AAD24380.1 Sequence problems. CP002685 Genomic DNA Translation: AEC06977.1 CP002685 Genomic DNA Translation: AEC06978.1 CP002685 Genomic DNA Translation: AEC06979.1 CP002685 Genomic DNA Translation: ANM61700.1 CP002685 Genomic DNA Translation: ANM61701.1 CP002685 Genomic DNA Translation: ANM61702.1 CP002685 Genomic DNA Translation: ANM61703.1 AK228820 mRNA Translation: BAF00716.1 BT029775 mRNA Translation: ABM06045.1 |
PIRi | A84586 |
RefSeqi | NP_001189559.1, NM_001202630.2 [Q8GZM7-1] NP_001318252.1, NM_001335663.1 [Q8GZM7-1] NP_001323902.1, NM_001335664.1 [Q8GZM7-1] NP_001323903.1, NM_001335665.1 [Q8GZM7-2] NP_001323904.1, NM_001335666.1 [Q8GZM7-1] NP_001323905.1, NM_001335667.1 [Q8GZM7-2] NP_849996.1, NM_179665.3 [Q8GZM7-2] |
3D structure databases
SMRi | Q8GZM7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1892, 24 interactors |
DIPi | DIP-61853N |
IntActi | Q8GZM7, 10 interactors |
STRINGi | 3702.AT2G20180.2 |
PTM databases
iPTMneti | Q8GZM7 |
Proteomic databases
PaxDbi | Q8GZM7 |
ProteomicsDBi | 235014 [Q8GZM7-1] |
Genome annotation databases
EnsemblPlantsi | AT2G20180.1; AT2G20180.1; AT2G20180 [Q8GZM7-2] AT2G20180.2; AT2G20180.2; AT2G20180 [Q8GZM7-1] AT2G20180.3; AT2G20180.3; AT2G20180 [Q8GZM7-1] AT2G20180.4; AT2G20180.4; AT2G20180 [Q8GZM7-1] AT2G20180.5; AT2G20180.5; AT2G20180 [Q8GZM7-2] AT2G20180.6; AT2G20180.6; AT2G20180 [Q8GZM7-1] AT2G20180.7; AT2G20180.7; AT2G20180 [Q8GZM7-2] |
GeneIDi | 816538 |
Gramenei | AT2G20180.1; AT2G20180.1; AT2G20180 [Q8GZM7-2] AT2G20180.2; AT2G20180.2; AT2G20180 [Q8GZM7-1] AT2G20180.3; AT2G20180.3; AT2G20180 [Q8GZM7-1] AT2G20180.4; AT2G20180.4; AT2G20180 [Q8GZM7-1] AT2G20180.5; AT2G20180.5; AT2G20180 [Q8GZM7-2] AT2G20180.6; AT2G20180.6; AT2G20180 [Q8GZM7-1] AT2G20180.7; AT2G20180.7; AT2G20180 [Q8GZM7-2] |
KEGGi | ath:AT2G20180 |
Organism-specific databases
Araporti | AT2G20180 |
TAIRi | locus:2061634, AT2G20180 |
Phylogenomic databases
eggNOGi | ENOG502QR6A, Eukaryota |
InParanoidi | Q8GZM7 |
OMAi | AQMGPRF |
OrthoDBi | 1384644at2759 |
PhylomeDBi | Q8GZM7 |
Miscellaneous databases
PROi | PR:Q8GZM7 |
Gene expression databases
ExpressionAtlasi | Q8GZM7, baseline and differential |
Genevisiblei | Q8GZM7, AT |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR031066, bHLH_ALC-like_plant IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR45855, PTHR45855, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIF1_ARATH | |
Accessioni | Q8GZM7Primary (citable) accession number: Q8GZM7 Secondary accession number(s): Q0WQ83, Q3EBY0, Q9SL63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 16, 2008 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names