UniProtKB - Q8GYQ5 (MPK12_ARATH)
Mitogen-activated protein kinase 12
MPK12
Functioni
Negative regulator of the auxin transduction signaling pathway (PubMed:19000167).
Involved in stomatal movement regulation by phosphorylating and repressing HT1 and HT1-mediated GHR1 phosphorylation (PubMed:27694184, PubMed:27923039).
Required for CO2-mediated stomatal movements (e.g. closure) (PubMed:27923039).
3 PublicationsCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 53 | Involved in HT1 binding1 Publication | 1 | |
Binding sitei | 70 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 167 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 47 – 55 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase activity Source: TAIR
- MAP kinase activity Source: TAIR
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
- protein tyrosine kinase activity Source: InterPro
GO - Biological processi
- auxin-activated signaling pathway Source: UniProtKB-KW
- cellular response to carbon dioxide Source: UniProtKB
- intracellular signal transduction Source: GO_Central
- protein autophosphorylation Source: UniProtKB
- regulation of stomatal closure Source: UniProtKB
- regulation of stomatal movement Source: UniProtKB
- response to auxin Source: TAIR
- response to indolebutyric acid Source: TAIR
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Auxin signaling pathway |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 121 Publication (EC:2.7.11.242 Publications)Short name: AtMPK121 Publication Short name: MAP kinase 121 Publication |
Gene namesi | Name:MPK121 Publication Ordered Locus Names:At2g46070Imported ORF Names:T3F17.28Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G46070 |
TAIRi | locus:2062897, AT2G46070 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Seems to translocate at the plasma membrane upon interaction with HT1.1 Publication
Nucleus
- nucleus Source: TAIR
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 53 | G → R: Increased stomatal opening and altered CO(2) and ozone (O(3)) responses. Weaker interaction with HT1. Strongly suppressed inhibition of HT1 activity. Loss of autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 70 | K → R: Loss of kinase activity. Reduced inhibition of HT1 activity. Loss of autophosphorylation. 2 Publications | 1 | |
Mutagenesisi | 122 | Y → C: Hyperactive kinase, can inhibit HT1 activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245812 | 1 – 372 | Mitogen-activated protein kinase 12Add BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 199 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 201 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 204 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8GYQ5 |
PRIDEi | Q8GYQ5 |
ProteomicsDBi | 238902 [Q8GYQ5-1] |
PTM databases
iPTMneti | Q8GYQ5 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q8GYQ5, baseline and differential |
Genevisiblei | Q8GYQ5, AT |
Interactioni
Subunit structurei
Binary interactionsi
Q8GYQ5
With | #Exp. | IntAct |
---|---|---|
IBR5 [Q84JU4] | 8 | EBI-2128461,EBI-604555 |
MKK1 [Q94A06] | 2 | EBI-2128461,EBI-994464 |
MKK6 [Q9FJV0] | 2 | EBI-2128461,EBI-1238868 |
MKK9 [Q9FX43] | 2 | EBI-2128461,EBI-2128545 |
Protein-protein interaction databases
BioGRIDi | 4550, 3 interactors |
IntActi | Q8GYQ5, 6 interactors |
STRINGi | 3702.AT2G46070.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 327 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 287 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 210 – 372 | IBR5 binding1 PublicationAdd BLAST | 163 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 199 – 201 | TXY | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
InParanoidi | Q8GYQ5 |
OMAi | DMKSERA |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q8GYQ5 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00219, TyrKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGESSSGST EHCIKVVPTH GGRYVQYNVY GQLFEVSRKY VPPIRPIGRG
60 70 80 90 100
ACGIVCAAVN SVTGEKVAIK KIGNAFDNII DAKRTLREIK LLRHMDHENV
110 120 130 140 150
ITIKDIVRPP QRDIFNDVYI VYELMDTDLQ RILRSNQTLT SDQCRFLVYQ
160 170 180 190 200
LLRGLKYVHS ANILHRDLRP SNVLLNSKNE LKIGDFGLAR TTSDTDFMTE
210 220 230 240 250
YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC ILGEIMTGQP LFPGKDYVHQ
260 270 280 290 300
LRLITELVGS PDNSSLGFLR SDNARRYVRQ LPRYPKQQFA ARFPKMPTTA
310 320 330 340 350
IDLLERMLVF DPNRRISVDE ALGHAYLSPH HDVAKEPVCS TPFSFDFEHP
360 370
SCTEEHIKEL IYKESVKFNP DH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4IH84 | F4IH84_ARATH | Mitogen-activated protein kinase 12 | MPK12 ATMPK12, MAPK12, At2g46070, T3F17.28 | 324 | Annotation score: | ||
A0A178VX75 | A0A178VX75_ARATH | Mitogen-activated protein kinase 12 | MPK12 ATMPK12, MAPK12, At2g46070, T3F17.28 | 406 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC005397 Genomic DNA Translation: AAC62906.1 Different initiation. CP002685 Genomic DNA Translation: AEC10639.1 AK117449 mRNA Translation: BAC42114.1 BT024898 mRNA Translation: ABD85169.1 |
PIRi | D84898 |
RefSeqi | NP_001325006.1, NM_001337177.1 NP_182131.2, NM_130170.4 [Q8GYQ5-1] |
Genome annotation databases
EnsemblPlantsi | AT2G46070.1; AT2G46070.1; AT2G46070 [Q8GYQ5-1] |
GeneIDi | 819215 |
Gramenei | AT2G46070.1; AT2G46070.1; AT2G46070 [Q8GYQ5-1] |
KEGGi | ath:AT2G46070 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC005397 Genomic DNA Translation: AAC62906.1 Different initiation. CP002685 Genomic DNA Translation: AEC10639.1 AK117449 mRNA Translation: BAC42114.1 BT024898 mRNA Translation: ABD85169.1 |
PIRi | D84898 |
RefSeqi | NP_001325006.1, NM_001337177.1 NP_182131.2, NM_130170.4 [Q8GYQ5-1] |
3D structure databases
AlphaFoldDBi | Q8GYQ5 |
SMRi | Q8GYQ5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4550, 3 interactors |
IntActi | Q8GYQ5, 6 interactors |
STRINGi | 3702.AT2G46070.1 |
PTM databases
iPTMneti | Q8GYQ5 |
Proteomic databases
PaxDbi | Q8GYQ5 |
PRIDEi | Q8GYQ5 |
ProteomicsDBi | 238902 [Q8GYQ5-1] |
Genome annotation databases
EnsemblPlantsi | AT2G46070.1; AT2G46070.1; AT2G46070 [Q8GYQ5-1] |
GeneIDi | 819215 |
Gramenei | AT2G46070.1; AT2G46070.1; AT2G46070 [Q8GYQ5-1] |
KEGGi | ath:AT2G46070 |
Organism-specific databases
Araporti | AT2G46070 |
TAIRi | locus:2062897, AT2G46070 |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
InParanoidi | Q8GYQ5 |
OMAi | DMKSERA |
OrthoDBi | 741207at2759 |
PhylomeDBi | Q8GYQ5 |
Miscellaneous databases
PROi | PR:Q8GYQ5 |
Gene expression databases
ExpressionAtlasi | Q8GYQ5, baseline and differential |
Genevisiblei | Q8GYQ5, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00219, TyrKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MPK12_ARATH | |
Accessioni | Q8GYQ5Primary (citable) accession number: Q8GYQ5 Secondary accession number(s): O82361 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families