UniProtKB - Q8GXU8 (LPAT1_ARATH)
1-acyl-sn-glycerol-3-phosphate acyltransferase LPAT1, chloroplastic
LPAT1
Functioni
Plastidial enzyme of the prokaryotic glycerol-3-phosphate pathway that converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at position sn-2 (PubMed:15169931).
Utilizes palmitoyl-ACP (16:0-ACP) to produce phosphatidic acid containing a saturated group at position sn-2, which is characteristic of lipids synthesized by the prokaryotic pathway (PubMed:15169931).
In vitro, can use 16:0-CoA as acyl donor (PubMed:14976237).
Essential for embryo development during the transition from the globular to the heart stage when chloroplasts begin to form (PubMed:15169931, PubMed:14976237).
2 PublicationsCatalytic activityi
- a 1-acyl-sn-glycero-3-phosphate + a fatty acyl-[ACP] = a 1,2-diacyl-sn-glycero-3-phosphate + holo-[ACP]1 PublicationEC:2.3.1.n41 PublicationThis reaction proceeds in the forwardCurated direction.
- a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA1 PublicationEC:2.3.1.511 PublicationThis reaction proceeds in the forwardCurated direction.
: CDP-diacylglycerol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.Curated This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- 1-acylglycerol-3-phosphate O-acyltransferase activity Source: UniProtKB
GO - Biological processi
- CDP-diacylglycerol biosynthetic process Source: UniProtKB
- embryo development ending in seed dormancy Source: TAIR
- phosphatidic acid biosynthetic process Source: GO_Central
- phosphatidylglycerol biosynthetic process Source: TAIR
Keywordsi
Molecular function | Acyltransferase, Developmental protein, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.51, 399 |
UniPathwayi | UPA00557;UER00613 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:LPAT1Curated Synonyms:ATS21 Publication, EMB1995Curated, LPAAT11 Publication Ordered Locus Names:At4g30580Imported ORF Names:F17I23.80Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G30580 |
TAIRi | locus:2118681, AT4G30580 |
Subcellular locationi
Chloroplast
- chloroplast membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Chloroplast
- chloroplast Source: TAIR
- chloroplast envelope Source: TAIR
- chloroplast membrane Source: UniProtKB
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- plastid Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 127 – 147 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 224 – 244 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Chloroplast, Membrane, PlastidPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 230 | P → L in lpat1-4; suppression of the sensitivity of fab1 mutant plants to low temperature. 1 Publication | 1 | |
Mutagenesisi | 290 | A → T in lpat1-3; suppression of the sensitivity of fab1 mutant plants to low temperature. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 56 | ChloroplastSequence analysisAdd BLAST | 56 | |
ChainiPRO_0000024701 | 57 – 356 | 1-acyl-sn-glycerol-3-phosphate acyltransferase LPAT1, chloroplasticAdd BLAST | 300 |
Proteomic databases
PaxDbi | Q8GXU8 |
PRIDEi | Q8GXU8 |
ProteomicsDBi | 238444 [Q8GXU8-1] |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
ExpressionAtlasi | Q8GXU8, baseline and differential |
Genevisiblei | Q8GXU8, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 14468, 1 interactor |
STRINGi | 3702.AT4G30580.1 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 202 – 207 | HXXXXD motifCurated | 6 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2848, Eukaryota |
HOGENOMi | CLU_027938_1_0_1 |
InParanoidi | Q8GXU8 |
OMAi | GWMMTLL |
OrthoDBi | 1623097at2759 |
PhylomeDBi | Q8GXU8 |
Family and domain databases
InterProi | View protein in InterPro IPR004552, AGP_acyltrans IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
TIGRFAMsi | TIGR00530, AGP_acyltrn, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDVASARSIS SHPSYYGKPI CSSQSSLIRI SRDKVCCFGR ISNGMTSFTT
60 70 80 90 100
SLHAVPSEKF MGETRRTGIQ WSNRSLRHDP YRFLDKKSPR SSQLARDITV
110 120 130 140 150
RADLSGAATP DSSFPEPEIK LSSRLRGIFF CVVAGISATF LIVLMIIGHP
160 170 180 190 200
FVLLFDPYRR KFHHFIAKLW ASISIYPFYK INIEGLENLP SSDTPAVYVS
210 220 230 240 250
NHQSFLDIYT LLSLGKSFKF ISKTGIFVIP IIGWAMSMMG VVPLKRMDPR
260 270 280 290 300
SQVDCLKRCM ELLKKGASVF FFPEGTRSKD GRLGSFKKGA FTVAAKTGVA
310 320 330 340 350
VVPITLMGTG KIMPTGSEGI LNHGNVRVII HKPIHGSKAD VLCNEARSKI
AESMDL
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013594 | 1 – 144 | Missing in isoform 2. 1 PublicationAdd BLAST | 144 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL161577 Genomic DNA Translation: CAB79776.1 CP002687 Genomic DNA Translation: AEE85783.1 AY136460 mRNA Translation: AAM97125.1 BT006253 mRNA Translation: AAP13361.1 AK118028 mRNA Translation: BAC42660.1 |
PIRi | G85357 |
RefSeqi | NP_194787.2, NM_119204.4 [Q8GXU8-1] |
Genome annotation databases
EnsemblPlantsi | AT4G30580.1; AT4G30580.1; AT4G30580 [Q8GXU8-1] |
GeneIDi | 829181 |
Gramenei | AT4G30580.1; AT4G30580.1; AT4G30580 [Q8GXU8-1] |
KEGGi | ath:AT4G30580 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL161577 Genomic DNA Translation: CAB79776.1 CP002687 Genomic DNA Translation: AEE85783.1 AY136460 mRNA Translation: AAM97125.1 BT006253 mRNA Translation: AAP13361.1 AK118028 mRNA Translation: BAC42660.1 |
PIRi | G85357 |
RefSeqi | NP_194787.2, NM_119204.4 [Q8GXU8-1] |
3D structure databases
AlphaFoldDBi | Q8GXU8 |
SMRi | Q8GXU8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 14468, 1 interactor |
STRINGi | 3702.AT4G30580.1 |
Proteomic databases
PaxDbi | Q8GXU8 |
PRIDEi | Q8GXU8 |
ProteomicsDBi | 238444 [Q8GXU8-1] |
Genome annotation databases
EnsemblPlantsi | AT4G30580.1; AT4G30580.1; AT4G30580 [Q8GXU8-1] |
GeneIDi | 829181 |
Gramenei | AT4G30580.1; AT4G30580.1; AT4G30580 [Q8GXU8-1] |
KEGGi | ath:AT4G30580 |
Organism-specific databases
Araporti | AT4G30580 |
TAIRi | locus:2118681, AT4G30580 |
Phylogenomic databases
eggNOGi | KOG2848, Eukaryota |
HOGENOMi | CLU_027938_1_0_1 |
InParanoidi | Q8GXU8 |
OMAi | GWMMTLL |
OrthoDBi | 1623097at2759 |
PhylomeDBi | Q8GXU8 |
Enzyme and pathway databases
UniPathwayi | UPA00557;UER00613 |
BRENDAi | 2.3.1.51, 399 |
Miscellaneous databases
PROi | PR:Q8GXU8 |
Gene expression databases
ExpressionAtlasi | Q8GXU8, baseline and differential |
Genevisiblei | Q8GXU8, AT |
Family and domain databases
InterProi | View protein in InterPro IPR004552, AGP_acyltrans IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
TIGRFAMsi | TIGR00530, AGP_acyltrn, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LPAT1_ARATH | |
Accessioni | Q8GXU8Primary (citable) accession number: Q8GXU8 Secondary accession number(s): Q9M0A2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families