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Protein

Probable isoaspartyl peptidase/L-asparaginase 2

Gene

At3g16150

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in asparagine catabolism and also in the final steps of protein degradation via hydrolysis of a range of isoaspartyl dipeptides.By similarity

Catalytic activityi

Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei195NucleophileBy similarity1

GO - Molecular functioni

  • asparaginase activity Source: TAIR
  • beta-aspartyl-peptidase activity Source: GO_Central
  • hydrolase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT3G16150-MONOMER
BRENDAi3.5.1.1 399
SABIO-RKiQ8GXG1

Protein family/group databases

MEROPSiT02.A02

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoaspartyl peptidase/L-asparaginase 2 (EC:3.4.19.5)
Alternative name(s):
L-asparagine amidohydrolase 2
Cleaved into the following 2 chains:
Gene namesi
Ordered Locus Names:At3g16150
ORF Names:MSL1.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G16150
TAIRilocus:2093387 AT3G16150

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000454441 – 194Isoaspartyl peptidase/L-asparaginase 2 subunit alphaAdd BLAST194
ChainiPRO_0000045445195 – 325Isoaspartyl peptidase/L-asparaginase 2 subunit betaAdd BLAST131

Post-translational modificationi

Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei194 – 195Cleavage; by autolysisBy similarity2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiQ8GXG1
PRIDEiQ8GXG1

Expressioni

Gene expression databases

ExpressionAtlasiQ8GXG1 baseline and differential
GenevisibleiQ8GXG1 AT

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G16150.1

Structurei

3D structure databases

ProteinModelPortaliQ8GXG1
SMRiQ8GXG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni223 – 226Substrate bindingBy similarity4
Regioni245 – 248Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the Ntn-hydrolase family.Curated

Phylogenomic databases

eggNOGiKOG1592 Eukaryota
COG1446 LUCA
HOGENOMiHOG000174613
InParanoidiQ8GXG1
KOiK13051
OMAiIRYQVAG
OrthoDBiEOG09360LPG
PhylomeDBiQ8GXG1

Family and domain databases

InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000246 Peptidase_T2
PANTHERiPTHR10188 PTHR10188, 1 hit
PfamiView protein in Pfam
PF01112 Asparaginase_2, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8GXG1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGGWAIAVHG GAGIDPNLPA ERQEEAKQLL TRCLNLGIIA LRSNVSAIDV
60 70 80 90 100
VELVIRELET DPLFNSGRGS ALTEKGTVEM EASIMDGTKR RCGAVSGITT
110 120 130 140 150
VKNPISLARL VMDKSPHSYL AFSGAEDFAR KQGVEIVDNE YFVTDDNVGM
160 170 180 190 200
LKLAKEANSI LFDYRIPPMG CAGAAATDSP IQMNGLPISI YAPETVGCVV
210 220 230 240 250
VDGKGHCAAG TSTGGLMNKM MGRIGDSPLI GAGTYASEFC GVSCTGEGEA
260 270 280 290 300
IIRATLARDV SAVMEYKGLN LQEAVDYVIK HRLDEGFAGL IAVSNKGEVV
310 320
CGFNSNGMFR GCATEDGFME VAIWE
Length:325
Mass (Da):34,341
Last modified:January 10, 2006 - v2
Checksum:i91641FA109B50519
GO
Isoform 2 (identifier: Q8GXG1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:176
Mass (Da):18,384
Checksum:i038D8E78BBDCD299
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0169361 – 149Missing in isoform 2. 1 PublicationAdd BLAST149

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012247 Genomic DNA Translation: BAB02681.1
CP002686 Genomic DNA Translation: AEE75777.1
AK118259 mRNA Translation: BAC42877.1
AY086130 mRNA Translation: AAM63335.1
RefSeqiNP_566536.1, NM_112485.4 [Q8GXG1-1]
UniGeneiAt.38938

Genome annotation databases

EnsemblPlantsiAT3G16150.1; AT3G16150.1; AT3G16150 [Q8GXG1-1]
GeneIDi820860
GrameneiAT3G16150.1; AT3G16150.1; AT3G16150 [Q8GXG1-1]
KEGGiath:AT3G16150

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012247 Genomic DNA Translation: BAB02681.1
CP002686 Genomic DNA Translation: AEE75777.1
AK118259 mRNA Translation: BAC42877.1
AY086130 mRNA Translation: AAM63335.1
RefSeqiNP_566536.1, NM_112485.4 [Q8GXG1-1]
UniGeneiAt.38938

3D structure databases

ProteinModelPortaliQ8GXG1
SMRiQ8GXG1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G16150.1

Protein family/group databases

MEROPSiT02.A02

Proteomic databases

PaxDbiQ8GXG1
PRIDEiQ8GXG1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G16150.1; AT3G16150.1; AT3G16150 [Q8GXG1-1]
GeneIDi820860
GrameneiAT3G16150.1; AT3G16150.1; AT3G16150 [Q8GXG1-1]
KEGGiath:AT3G16150

Organism-specific databases

AraportiAT3G16150
TAIRilocus:2093387 AT3G16150

Phylogenomic databases

eggNOGiKOG1592 Eukaryota
COG1446 LUCA
HOGENOMiHOG000174613
InParanoidiQ8GXG1
KOiK13051
OMAiIRYQVAG
OrthoDBiEOG09360LPG
PhylomeDBiQ8GXG1

Enzyme and pathway databases

BioCyciARA:AT3G16150-MONOMER
BRENDAi3.5.1.1 399
SABIO-RKiQ8GXG1

Miscellaneous databases

PROiPR:Q8GXG1

Gene expression databases

ExpressionAtlasiQ8GXG1 baseline and differential
GenevisibleiQ8GXG1 AT

Family and domain databases

InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000246 Peptidase_T2
PANTHERiPTHR10188 PTHR10188, 1 hit
PfamiView protein in Pfam
PF01112 Asparaginase_2, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASPGB_ARATH
AccessioniPrimary (citable) accession number: Q8GXG1
Secondary accession number(s): Q9LW72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: November 7, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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