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Protein

Potassium channel AKT6

Gene

AKT6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Highly selective inward-rectifying potassium channel that could mediate potassium uptake in the pollen membrane. Plays an important role in pollen tube development. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. May interact with the cytoskeleton or with regulatory proteins.1 Publication

Caution

Was originally erroneously termed AKT5.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi398 – 519cNMPAdd BLAST122

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Protein family/group databases

TCDBi1.A.1.4.8 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel AKT6
Alternative name(s):
Potassium channel SPIK
Shaker pollen inward rectifier K(+) channel
Gene namesi
Name:AKT6
Synonyms:SPIK
Ordered Locus Names:At2g25600
ORF Names:F3N11.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G25600
TAIRilocus:2050276 AT2G25600

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 84CytoplasmicSequence analysisAdd BLAST84
Transmembranei85 – 105Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini106 – 113ExtracellularSequence analysis8
Transmembranei114 – 134Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini135 – 155CytoplasmicSequence analysisAdd BLAST21
Transmembranei156 – 176Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini177 – 184ExtracellularSequence analysis8
Transmembranei185 – 205Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini206 – 219CytoplasmicSequence analysisAdd BLAST14
Transmembranei220 – 240Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini241 – 267ExtracellularSequence analysisAdd BLAST27
Intramembranei268 – 287Pore-forming; Name=Segment H5Sequence analysisAdd BLAST20
Topological domaini288 – 291ExtracellularSequence analysis4
Transmembranei292 – 312Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini313 – 888CytoplasmicSequence analysisAdd BLAST576

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541241 – 888Potassium channel AKT6Add BLAST888

Proteomic databases

PaxDbiQ8GXE6
PRIDEiQ8GXE6

Expressioni

Tissue specificityi

Predominantly expressed in flowers; especially in pollen.2 Publications

Gene expression databases

ExpressionAtlasiQ8GXE6 baseline and differential
GenevisibleiQ8GXE6 AT

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.By similarity

Protein-protein interaction databases

BioGridi2451, 7 interactors
IntActiQ8GXE6, 7 interactors
STRINGi3702.AT2G25600.1

Structurei

3D structure databases

ProteinModelPortaliQ8GXE6
SMRiQ8GXE6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati543 – 572ANK 1Add BLAST30
Repeati576 – 605ANK 2Add BLAST30
Repeati609 – 638ANK 3Add BLAST30
Repeati640 – 669ANK 4Add BLAST30
Repeati673 – 702ANK 5Add BLAST30
Domaini822 – 888KHAPROSITE-ProRule annotationAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 24Poly-Gly10

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
HOGENOMiHOG000238230
InParanoidiQ8GXE6
KOiK21867
OMAiGSHYCVV
OrthoDBiEOG093601WX
PhylomeDBiQ8GXE6

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00038 CAP_ED, 1 hit
Gene3Di1.25.40.20, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR005821 Ion_trans_dom
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR021789 KHA_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF11834 KHA, 1 hit
PRINTSiPR01463 EAGCHANLFMLY
SMARTiView protein in SMART
SM00248 ANK, 4 hits
SM00100 cNMP, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
PS50042 CNMP_BINDING_3, 1 hit
PS51490 KHA, 1 hit

Sequencei

Sequence statusi: Complete.

Q8GXE6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKKKVWFWG VKDDGEGGGG RGGGRTKDAE DDVADHLSRD GTMSQYSLSK
60 70 80 90 100
GLLPSLGANN RSSRDVILPR FIVSPFDPRY RAWETFLVFL VLYTAWASPF
110 120 130 140 150
EFGFLQKPRP PLSILDNIVN GFFAVDIVLT FFVAFLDKVT YLLVDDPKRI
160 170 180 190 200
AWRYASTWLI FDVVSTFPYE IFGSLLHESI QGYGIFSMLR LWRLRRVSNC
210 220 230 240 250
FARLEKDRKY SYFWVRCSKL LLVTLFVIHC GACFLYSIAA HYPDPSKTFM
260 270 280 290 300
ALTDENWKES PIAVRYNTAM YWSITTFSTT GYGDIHGVNS REMTFILFYM
310 320 330 340 350
VFNLGLSAYI IGNMTNLVVH VTGRTRKFRD TIQAASGFGQ RNNLPVRLQD
360 370 380 390 400
QMVAHLCLRY RTDSEGLQQQ EIIDSLPKAI RSSISHYLFY EVVDKIYLFH
410 420 430 440 450
GISNDLLFQL VTEMKAEYFP PKEDVILQNE APTDFYILVT GAVDIIARVN
460 470 480 490 500
GVEQVVSEAQ RGHVFGEVGV LCYRPQLFTV RTKRLSQLLR LNRTVLLNLV
510 520 530 540 550
QANVGDGAII MNNLLQHLKD SEDPVMKGVL ADTEHMLAQG KMDLPLSLCF
560 570 580 590 600
AAARGDDLLL HQLLRRGSSP NEMDKDGRTA LHIAASKGSH YCVVLLLEHG
610 620 630 640 650
ADPNIRDSEG NVPLWEAIIG RHREIAKLLA ENGAKLSLDS VSYFSGLAVE
660 670 680 690 700
KNCLDALKDI IKYGGDVTLP DGNGTTALHR AVSEGHLEIV KFLLDQGADL
710 720 730 740 750
DWPDSYGWTP RGLADHQGNE EIKTLFHNHR PVEKKPKPIP GIPQSPVTGK
760 770 780 790 800
PLMKYSSEPT MHSGELVLDG GQVVVSQKRK LNNFRNSLFG IISAANSADD
810 820 830 840 850
GGEVPRSPAV PGGGGSMIYP ERVTISSPEN GETGGKVVLL PNSMEELLKI
860 870 880
GENKMGFVPT KVLTREGAEI DDITLIRDGD FLLLSRDP
Length:888
Mass (Da):99,215
Last modified:March 1, 2004 - v2
Checksum:i60479CE59404505E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309323 Genomic DNA Translation: CAC85283.1
AC006053 Genomic DNA Translation: AAD31377.1
CP002685 Genomic DNA Translation: AEC07722.1
AK118279 mRNA Translation: BAC42897.1
PIRiD84650
RefSeqiNP_180131.3, NM_128118.5
UniGeneiAt.39001

Genome annotation databases

EnsemblPlantsiAT2G25600.1; AT2G25600.1; AT2G25600
GeneIDi817099
GrameneiAT2G25600.1; AT2G25600.1; AT2G25600
KEGGiath:AT2G25600

Similar proteinsi

Entry informationi

Entry nameiAKT6_ARATH
AccessioniPrimary (citable) accession number: Q8GXE6
Secondary accession number(s): Q9SLA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: July 18, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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