UniProtKB - Q8GXB7 (TRMB_ARATH)
Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
At5g24840
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation
Catalytic activityi
- guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.33UniRule annotation
: N(7)-methylguanine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 71 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 148 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 151 | UniRule annotation | 1 |
GO - Molecular functioni
- tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
- tRNA binding Source: UniProtKB-UniRule
GO - Biological processi
- RNA (guanine-N7)-methylation Source: GO_Central
- tRNA methylation Source: GO_Central
- tRNA modification Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)Alternative name(s): tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation tRNA(m7G46)-methyltransferaseUniRule annotation |
Gene namesi | Ordered Locus Names:At5g24840 ORF Names:F6A4_50 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G24840 |
TAIRi | locus:2149443, AT5G24840 |
Subcellular locationi
Nucleus
- Nucleus UniRule annotation
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- tRNA methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171435 | 1 – 251 | tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST | 251 |
Proteomic databases
PaxDbi | Q8GXB7 |
PRIDEi | Q8GXB7 |
ProteomicsDBi | 245243 |
PTM databases
iPTMneti | Q8GXB7 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q8GXB7, baseline and differential |
Genevisiblei | Q8GXB7, AT |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 94 – 95 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 128 – 129 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 226 – 228 | S-adenosyl-L-methionine bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
HOGENOMi | CLU_050910_3_0_1 |
InParanoidi | Q8GXB7 |
OMAi | KWRIINQ |
OrthoDBi | 1417652at2759 |
PhylomeDBi | Q8GXB7 |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit PTHR23417:SF16, PTHR23417:SF16, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8GXB7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNETKATTF SKSTGLPRKR FYRARAHSNP LSDSHFPIPI SPAHVDYSLH
60 70 80 90 100
FPKFVEADNK FIKKVEFADI GCGFGGLLIS LATLFPDTLM IGMELRDKVT
110 120 130 140 150
EYVKERILAL RRTSSEGQYE NISVVRTNSM KYIPNYFEKG QLSKMFFLFP
160 170 180 190 200
DPHFKEKNHR RRVISTHLLD EYAYVLRAGG IIYTITDVEE LGEWMKSCLE
210 220 230 240 250
KHPMFESLTQ EELVSDPVVE LLCSATEEGQ KVARNGGQTF RAVFRRIAYV
S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF069716 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED93368.1 AK118324 mRNA Translation: BAC42938.1 BT006128 mRNA Translation: AAP04113.1 |
RefSeqi | NP_197866.1, NM_122393.3 |
Genome annotation databases
EnsemblPlantsi | AT5G24840.1; AT5G24840.1; AT5G24840 |
GeneIDi | 832553 |
Gramenei | AT5G24840.1; AT5G24840.1; AT5G24840 |
KEGGi | ath:AT5G24840 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF069716 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED93368.1 AK118324 mRNA Translation: BAC42938.1 BT006128 mRNA Translation: AAP04113.1 |
RefSeqi | NP_197866.1, NM_122393.3 |
3D structure databases
SMRi | Q8GXB7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G24840.1 |
PTM databases
iPTMneti | Q8GXB7 |
Proteomic databases
PaxDbi | Q8GXB7 |
PRIDEi | Q8GXB7 |
ProteomicsDBi | 245243 |
Genome annotation databases
EnsemblPlantsi | AT5G24840.1; AT5G24840.1; AT5G24840 |
GeneIDi | 832553 |
Gramenei | AT5G24840.1; AT5G24840.1; AT5G24840 |
KEGGi | ath:AT5G24840 |
Organism-specific databases
Araporti | AT5G24840 |
TAIRi | locus:2149443, AT5G24840 |
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
HOGENOMi | CLU_050910_3_0_1 |
InParanoidi | Q8GXB7 |
OMAi | KWRIINQ |
OrthoDBi | 1417652at2759 |
PhylomeDBi | Q8GXB7 |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Miscellaneous databases
PROi | PR:Q8GXB7 |
Gene expression databases
ExpressionAtlasi | Q8GXB7, baseline and differential |
Genevisiblei | Q8GXB7, AT |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit PTHR23417:SF16, PTHR23417:SF16, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMB_ARATH | |
Accessioni | Q8GXB7Primary (citable) accession number: Q8GXB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families