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Protein

Probable pectinesterase/pectinesterase inhibitor 23

Gene

PME23

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At5g45990), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At3g06710), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g50310), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At3g31890), Pectinesterase (PMEPCRF), Pectinesterase (At3g10720), Putative pectinesterase 10 (PME10), Putative pectinesterase 11 (PME11), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 14 (PME14), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase/pectinesterase inhibitor 18 (PME18), Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Pectinesterase 1 (PME1), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase 29 (PME29), Pectinesterase 2 (PME2), Probable pectinesterase 30 (PME30), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase/pectinesterase inhibitor 3 (PME3), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Pectinesterase 4 (PME4), Probable pectinesterase 50 (PME50), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Putative pectinesterase 52 (PME52), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Probable pectinesterase 55 (PME55), Probable pectinesterase 56 (PME56), Putative pectinesterase 57 (PME57), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Pectinesterase QRT1 (QRT1), Putative pectinesterase 63 (PME63), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Probable pectinesterase 66 (PME66), Probable pectinesterase 67 (PME67), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 8 (PME8), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At3g30550), Pectinesterase (At3g14310), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g18720), Pectinesterase (At4g15980), Pectinesterase (At3g49220), Pectinesterase (AXX17_At2g22270), Pectinesterase (AXX17_At4g38050), Uncharacterized protein (AXX17_At3g05180), Pectinesterase (AXX17_At5g47900), Pectinesterase, Pectinesterase (AXX17_At5g19650), Pectinesterase (AXX17_At3g43380), Pectinesterase (AXX17_At4g02900), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At3g10570), Pectinesterase (AXX17_At3g56460), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At1g11900), PME26 (AXX17_At3g14890), Uncharacterized protein (AXX17_At5g27870), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (AXX17_At1g04730), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At5g04430), Pectinesterase, Pectinesterase (F14I3.7), Pectinesterase (AXX17_At2g22280), Pectinesterase, Pectinesterase (AXX17_At5g52320), Pectinesterase (AXX17_At2g33420), Pectinesterase, Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At2g40510), Pectinesterase (At5g20860), Pectinesterase (AXX17_At3g26070), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g38040), Pectinesterase (AXX17_At2g33410), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At3g05190), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At4g02930), Pectinesterase (AXX17_At3g17940), Pectinesterase (AXX17_At2g45340), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (At5g26810), Pectinesterase (PME44), Pectinesterase (At1g53840)
  2. Pectate lyase (AXX17_At3g06910), Pectate lyase (At3g27400), Pectate lyase (AXX17_At3g26200), Pectate lyase (AXX17_At3g09180), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At3g49720), Pectate lyase (AXX17_At4g25610), Putative pectate lyase 14 (At4g13210), Probable pectate lyase 19 (At5g15110), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 6 (At2g02720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 12 (At3g53190), Putative pectate lyase 17 (At4g22090), Probable pectate lyase 10 (At3g24670), Probable pectate lyase 13 (PMR6), Probable pectate lyase 1 (At1g04680), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 16 (At4g22080), Probable pectate lyase 18 (At4g24780), Putative pectate lyase 21 (At5g55720), Probable pectate lyase 22 (At5g63180), Putative pectate lyase 2 (At1g11920), Putative pectate lyase 11 (At3g27400), Probable pectate lyase 15 (At4g13710), Pectate lyase (At5g09280), Pectate lyase (At3g55140), Pectate lyase, Pectate lyase (At1g14420), Pectate lyase (F11F8_12), Pectate lyase (At5g04310), Pectate lyase (AXX17_At3g47590), Pectate lyase (At3g09540), Pectate lyase (AXX17_At5g54880), Pectate lyase, Pectate lyase (AXX17_At5g08800), Pectate lyase (AXX17_At1g04020), Pectate lyase (AXX17_At1g15010), Pectate lyase (AXX17_At1g12300), Pectate lyase (At3g01270), Pectate lyase (AXX17_At2g01680), Pectate lyase (At3g55140), Pectate lyase (AXX17_At3g00310), Pectate lyase (AXX17_At5g47570), Pectate lyase (AXX17_At5g03700), Pectate lyase (AXX17_At1g30780), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At3g29900), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g14600), Pectate lyase (AXX17_At1g61710), Pectate lyase (AXX17_At5g62760), Pectate lyase (At3g07010), Pectate lyase (At3g01270), Pectate lyase, Pectate lyase (AXX17_At4g25600), Pectate lyase (At5g04310), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At4g15660), Pectate lyase (At3g53190), Pectate lyase, Pectate lyase (At4g13710), Pectate lyase (At4g13210), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g26720), Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei333Substrate; for pectinesterase activityBy similarity1
Binding sitei363Substrate; for pectinesterase activityBy similarity1
Sitei385Transition state stabilizerBy similarity1
Active sitei386Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei407Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei475Substrate; for pectinesterase activityBy similarity1
Binding sitei477Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: GO_Central
  • pectinesterase inhibitor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G06830-MONOMER
BRENDAi3.1.1.11 399
UniPathwayi
UPA00545;UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 23
Including the following 2 domains:
Pectinesterase inhibitor 23
Alternative name(s):
Pectin methylesterase inhibitor 23
Pectinesterase 23 (EC:3.1.1.11)
Short name:
PE 23
Alternative name(s):
Pectin methylesterase 23
Short name:
AtPME23
Gene namesi
Name:PME23
Synonyms:ARATH23
Ordered Locus Names:At3g06830
ORF Names:F3E22.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G06830
TAIRilocus:2083308 AT3G06830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000037167934 – 568Probable pectinesterase/pectinesterase inhibitor 23Add BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi210N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi400 ↔ 420By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8GXA1

Expressioni

Tissue specificityi

Expressed in mature pollen grains in the anthers and on the stigma. Found in pollen tubes within the style.1 Publication

Gene expression databases

ExpressionAtlasiQ8GXA1 baseline and differential
GenevisibleiQ8GXA1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G06830.1

Structurei

3D structure databases

ProteinModelPortaliQ8GXA1
SMRiQ8GXA1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 198Pectinesterase inhibitor 23Add BLAST154
Regioni251 – 548Pectinesterase 23Add BLAST298

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGWD Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ8GXA1
OrthoDBiEOG093609DH
PhylomeDBiQ8GXA1

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
IPR018040 Pectinesterase_Tyr_AS
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00800 PECTINESTERASE_1, 1 hit
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8GXA1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSDGDKKKK FIVAGSVSGF LVIMVVSVAV VTSKHSPKDD ENHIRKTTKA
60 70 80 90 100
VQAVCAPTDF KDTCVNSLMG ASPDSDDPVD LIKLGFKVTI KSINESLEKA
110 120 130 140 150
SGDIKAKADK NPEAKGAFEL CEKLMIDAID DLKKCMDHGF SVDQIEVFVE
160 170 180 190 200
DLRVWLSGSI AFQQTCMDSF GEIKSNLMQD MLKIFKTSRE LSSNSLAMVT
210 220 230 240 250
RISTLIPNSN LTGLTGALAK YARKLLSTED SIPTWVGPEA RRLMAAQGGG
260 270 280 290 300
PGPVKANAVV AQDGTGQFKT ITDALNAVPK GNKVPFIIHI KEGIYKEKVT
310 320 330 340 350
VTKKMPHVTF IGDGPNKTLI TGSLNFGIGK VKTFLTATIT IEGDHFTAKN
360 370 380 390 400
IGIENTAGPE GGQAVALRVS ADYAVFHSCQ IDGHQDTLYV HSHRQFYRDC
410 420 430 440 450
TVSGTVDFIF GDAKCILQNC KIVVRKPNKG QTCMVTAQGR SNVRESTGLV
460 470 480 490 500
LHGCHITGDP AYIPMKSVNK AYLGRPWKEF SRTIIMKTTI DDVIDPAGWL
510 520 530 540 550
PWSGDFALKT LYYAEHMNTG PGSNQAQRVK WPGIKKLTPQ DALLYTGDRF
560
LRGDTWIPQT QVPYTAKV
Length:568
Mass (Da):61,886
Last modified:June 16, 2009 - v3
Checksum:iFE92C797055361A7
GO

Sequence cautioni

The sequence AAF63815 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107K → E in BAC42959 (PubMed:11910074).Curated1
Sequence conflicti568V → A in BAC42959 (PubMed:11910074).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC023912 Genomic DNA Translation: AAF63815.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE74464.1
AK118345 mRNA Translation: BAC42959.2
BT030353 mRNA Translation: ABO38766.1
RefSeqiNP_187339.2, NM_111563.4
UniGeneiAt.40454

Genome annotation databases

EnsemblPlantsiAT3G06830.1; AT3G06830.1; AT3G06830
GeneIDi819867
GrameneiAT3G06830.1; AT3G06830.1; AT3G06830
KEGGiath:AT3G06830

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC023912 Genomic DNA Translation: AAF63815.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE74464.1
AK118345 mRNA Translation: BAC42959.2
BT030353 mRNA Translation: ABO38766.1
RefSeqiNP_187339.2, NM_111563.4
UniGeneiAt.40454

3D structure databases

ProteinModelPortaliQ8GXA1
SMRiQ8GXA1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G06830.1

Proteomic databases

PaxDbiQ8GXA1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G06830.1; AT3G06830.1; AT3G06830
GeneIDi819867
GrameneiAT3G06830.1; AT3G06830.1; AT3G06830
KEGGiath:AT3G06830

Organism-specific databases

AraportiAT3G06830
TAIRilocus:2083308 AT3G06830

Phylogenomic databases

eggNOGiENOG410IGWD Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ8GXA1
OrthoDBiEOG093609DH
PhylomeDBiQ8GXA1

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

BioCyciARA:AT3G06830-MONOMER
BRENDAi3.1.1.11 399

Miscellaneous databases

PROiPR:Q8GXA1

Gene expression databases

ExpressionAtlasiQ8GXA1 baseline and differential
GenevisibleiQ8GXA1 AT

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
IPR018040 Pectinesterase_Tyr_AS
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00800 PECTINESTERASE_1, 1 hit
PS00503 PECTINESTERASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPME23_ARATH
AccessioniPrimary (citable) accession number: Q8GXA1
Secondary accession number(s): A4FVQ5, Q9M7Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 16, 2009
Last modified: November 7, 2018
This is version 96 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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