UniProtKB - Q8GWT4 (ANM15_ARATH)
Protein
Protein arginine N-methyltransferase 1.5
Gene
PMRT15
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Methylates arginine residues of myelin basic protein (MBP) in vitro. Methylates symmetrically histone H4 of the FLC chromatin to form H4R3me2s, which in turn suppresses FLC expression to induce flowering. Regulates alternative splicing by methylating spliceosomal proteins. Involved in the post-transcriptional regulation of the circadian clock.2 Publications
Catalytic activityi
- L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H+ + Nω,Nω-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteineEC:2.1.1.319
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 310 | Peptide substrateBy similarity | 1 | |
Binding sitei | 330 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 398 | S-adenosyl-L-methionineBy similarity | 1 | |
Active sitei | 442 | Proton donor/acceptorBy similarity | 1 | |
Active sitei | 451 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- histone-arginine N-methyltransferase activity Source: GO_Central
- protein-arginine omega-N asymmetric methyltransferase activity Source: UniProtKB-EC
- protein methyltransferase activity Source: TAIR
GO - Biological processi
- histone arginine methylation Source: GO_Central
- histone H4-R3 methylation Source: TAIR
- positive regulation of vernalization response Source: TAIR
- regulation of flower development Source: TAIR
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.125, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein arginine N-methyltransferase 1.5 (EC:2.1.1.319)Short name: AtPMRT15 Short name: AtPMRT5 Alternative name(s): Shk1 kinase-binding protein 1 homolog |
Gene namesi | Name:PMRT15 Synonyms:PMRT5, SKB1 Ordered Locus Names:At4g31120 ORF Names:F6E21.40 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G31120 |
TAIRi | locus:2126276, AT4G31120 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Plants are late-flowering. Increased alternative splicing of several genes, including APRR9.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000293993 | 1 – 642 | Protein arginine N-methyltransferase 1.5Add BLAST | 642 |
Proteomic databases
PaxDbi | Q8GWT4 |
PRIDEi | Q8GWT4 |
ProteomicsDBi | 245055 [Q8GWT4-1] |
Expressioni
Tissue specificityi
Highly expressed in flowers, roots and siliques, lower expression in stems and mature leaves (at protein level). Abundant in shoot apex, young leaves and leaf primordia, floral and inflorescence meristems, gynoecium, stamens, sepals and young siliques, but not in older leaves, petals and vascular tissues.1 Publication
Developmental stagei
High levels from 5 to 20 days after germination (at protein level).1 Publication
Inductioni
Circadian-regulation.1 Publication
Gene expression databases
ExpressionAtlasi | Q8GWT4, baseline and differential |
Genevisiblei | Q8GWT4, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 14527, 3 interactors |
STRINGi | 3702.AT4G31120.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 314 – 620 | SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST | 307 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 339 – 340 | S-adenosyl-L-methionine bindingBy similarity | 2 | |
Regioni | 426 – 427 | S-adenosyl-L-methionine bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0822, Eukaryota |
HOGENOMi | CLU_010247_3_0_1 |
InParanoidi | Q8GWT4 |
OMAi | FPMFFPT |
OrthoDBi | 475852at2759 |
PhylomeDBi | Q8GWT4 |
Family and domain databases
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR007857, Arg_MeTrfase_PRMT5 IPR035075, PRMT5 IPR035248, PRMT5_C IPR035247, PRMT5_TIM IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR10738, PTHR10738, 1 hit |
Pfami | View protein in Pfam PF05185, PRMT5, 1 hit PF17286, PRMT5_C, 1 hit PF17285, PRMT5_TIM, 1 hit |
PIRSFi | PIRSF015894, Skb1_MeTrfase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8GWT4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPLGERGGWE RTESRYCGVE TDFSNDVTHL LNFNISTGGF DYVLAPLVDP
60 70 80 90 100
SYRPSLVEGN GVDTQVLPVC GSDLVLSPSQ WSSHVVGKIS SWIDLDSEDE
110 120 130 140 150
VLRMDSETTL KQEIAWATHL SLQACLLPTP KGKSCANYAR CVNQILQGLT
160 170 180 190 200
TLQLWLRVPL VKSEGDSMDD TSEGLNDSWE LWNSFRLLCE HDSKLSVALD
210 220 230 240 250
VLSTLPSETS LGRWMGESVR AAILSTDAFL TNARGYPCLS KRHQKLIAGF
260 270 280 290 300
FDHAAQVVIC GKPVHNLQKP LDSSSEGTEK NPLRIYLDYV AYLFQKMESL
310 320 330 340 350
SEQERIELGY RDFLQAPLQP LMDNLEAQTY ETFERDSVKY IQYQRAVEKA
360 370 380 390 400
LVDRVPDEKA SELTTVLMVV GAGRGPLVRA SLQAAEETDR KLKVYAVEKN
410 420 430 440 450
PNAVVTLHNL VKMEGWEDVV TIISCDMRFW NAPEQADILV SELLGSFGDN
460 470 480 490 500
ELSPECLDGA QRFLKPDGIS IPSSYTSFIQ PITASKLYND VKAHKDLAHF
510 520 530 540 550
ETAYVVKLHS VAKLAPSQSV FTFTHPNFST KVNNQRYKKL QFSLPSDAGS
560 570 580 590 600
ALVHGFAGYF DSVLYKDVHL GIEPTTATPN MFSWFPIFFP LRKPVEVHPD
610 620 630 640
TPLEVHFWRC CGSSKVWYEW SVSSPTPSPM HNTNGRSYWV GL
Sequence cautioni
The sequence CAB79830 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 10 | E → G in AAO64842 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 10 | E → G in BAC43244 (PubMed:14593172).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027572 | 492 – 510 | KAHKD…VKLHS → RLIKILRTLKLLMLSSCIV in isoform 2. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_027573 | 511 – 642 | Missing in isoform 2. 1 PublicationAdd BLAST | 132 | |
Alternative sequenceiVSP_027574 | 585 – 642 | Missing in isoform 3. CuratedAdd BLAST | 58 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL161578 Genomic DNA Translation: CAB79830.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85860.1 CP002687 Genomic DNA Translation: AEE85861.1 BT005907 mRNA Translation: AAO64842.1 AK118648 mRNA Translation: BAC43244.1 |
PIRi | T10666 |
RefSeqi | NP_194841.2, NM_119262.6 [Q8GWT4-1] NP_974647.1, NM_202918.2 [Q8GWT4-3] |
Genome annotation databases
EnsemblPlantsi | AT4G31120.1; AT4G31120.1; AT4G31120 [Q8GWT4-1] AT4G31120.2; AT4G31120.2; AT4G31120 [Q8GWT4-3] |
GeneIDi | 829240 |
Gramenei | AT4G31120.1; AT4G31120.1; AT4G31120 [Q8GWT4-1] AT4G31120.2; AT4G31120.2; AT4G31120 [Q8GWT4-3] |
KEGGi | ath:AT4G31120 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL161578 Genomic DNA Translation: CAB79830.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85860.1 CP002687 Genomic DNA Translation: AEE85861.1 BT005907 mRNA Translation: AAO64842.1 AK118648 mRNA Translation: BAC43244.1 |
PIRi | T10666 |
RefSeqi | NP_194841.2, NM_119262.6 [Q8GWT4-1] NP_974647.1, NM_202918.2 [Q8GWT4-3] |
3D structure databases
SMRi | Q8GWT4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 14527, 3 interactors |
STRINGi | 3702.AT4G31120.1 |
Proteomic databases
PaxDbi | Q8GWT4 |
PRIDEi | Q8GWT4 |
ProteomicsDBi | 245055 [Q8GWT4-1] |
Genome annotation databases
EnsemblPlantsi | AT4G31120.1; AT4G31120.1; AT4G31120 [Q8GWT4-1] AT4G31120.2; AT4G31120.2; AT4G31120 [Q8GWT4-3] |
GeneIDi | 829240 |
Gramenei | AT4G31120.1; AT4G31120.1; AT4G31120 [Q8GWT4-1] AT4G31120.2; AT4G31120.2; AT4G31120 [Q8GWT4-3] |
KEGGi | ath:AT4G31120 |
Organism-specific databases
Araporti | AT4G31120 |
TAIRi | locus:2126276, AT4G31120 |
Phylogenomic databases
eggNOGi | KOG0822, Eukaryota |
HOGENOMi | CLU_010247_3_0_1 |
InParanoidi | Q8GWT4 |
OMAi | FPMFFPT |
OrthoDBi | 475852at2759 |
PhylomeDBi | Q8GWT4 |
Enzyme and pathway databases
BRENDAi | 2.1.1.125, 399 |
Miscellaneous databases
PROi | PR:Q8GWT4 |
Gene expression databases
ExpressionAtlasi | Q8GWT4, baseline and differential |
Genevisiblei | Q8GWT4, AT |
Family and domain databases
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR007857, Arg_MeTrfase_PRMT5 IPR035075, PRMT5 IPR035248, PRMT5_C IPR035247, PRMT5_TIM IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR10738, PTHR10738, 1 hit |
Pfami | View protein in Pfam PF05185, PRMT5, 1 hit PF17286, PRMT5_C, 1 hit PF17285, PRMT5_TIM, 1 hit |
PIRSFi | PIRSF015894, Skb1_MeTrfase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ANM15_ARATH | |
Accessioni | Q8GWT4Primary (citable) accession number: Q8GWT4 Secondary accession number(s): Q9M090 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 10, 2007 |
Last sequence update: | July 10, 2007 | |
Last modified: | December 2, 2020 | |
This is version 112 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families