UniProtKB - Q8GW31 (DCP2_ARATH)
mRNA-decapping enzyme subunit 2
DCP2
Functioni
Catalytic component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Essential for postembryonic development, especially during the formation of the shoot apical meristem (SAM).
4 PublicationsCatalytic activityi
- a 5'-end (N7-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + 2 H+ + N7-methyl-GDP1 PublicationEC:3.6.1.621 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 154 | MagnesiumBy similarity | 1 | |
Metal bindingi | 158 | MagnesiumBy similarity | 1 | |
Binding sitei | 178 | ATPBy similarity | 1 | |
Binding sitei | 233 | ATPBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- m7G(5')pppN diphosphatase activity Source: TAIR
- magnesium ion binding Source: UniProtKB
- manganese ion binding Source: UniProtKB
- mRNA binding Source: TAIR
- protein homodimerization activity Source: TAIR
GO - Biological processi
- deadenylation-dependent decapping of nuclear-transcribed mRNA Source: GO_Central
- deadenylation-independent decapping of nuclear-transcribed mRNA Source: TAIR
- modulation by virus of host process Source: TAIR
- mRNA catabolic process Source: TAIR
- mRNA processing Source: UniProtKB-KW
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: InterPro
- post-embryonic development Source: UniProtKB
- posttranscriptional gene silencing Source: TAIR
- primary shoot apical meristem specification Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, RNA-binding |
Biological process | mRNA processing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: mRNA-decapping enzyme subunit 2 (EC:3.6.1.621 Publication)Short name: AtDCP2 Short name: Protein DECAPPING 2 Alternative name(s): M(7)GpppN-mRNA hydrolase DCP2 Protein TRIDENT |
Gene namesi | Name:DCP2 Synonyms:TDT Ordered Locus Names:At5g13570 ORF Names:MSH12.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G13570 |
TAIRi | locus:2173174, AT5G13570 |
Subcellular locationi
Other locations
- P-body 3 Publications
Note: The localization to P-body is VCS-dependent.
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 154 | E → Q: Loss of mRNA decaping activity. 1 Publication | 1 | |
Mutagenesisi | 158 | E → Q: Loss of mRNA decaping activity. 2 Publications | 1 | |
Mutagenesisi | 222 – 232 | Missing : Loss of mRNA decaping activity. 1 PublicationAdd BLAST | 11 | |
Mutagenesisi | 226 | G → A: Reduced mRNA decaping activity. 1 Publication | 1 | |
Mutagenesisi | 231 | K → A: Reduced mRNA decaping activity. 1 Publication | 1 | |
Mutagenesisi | 243 | K → A: Reduced mRNA decaping activity. 1 Publication | 1 | |
Mutagenesisi | 248 | K → A: Reduced mRNA decaping activity. 1 Publication | 1 | |
Mutagenesisi | 372 – 373 | SA → PD: Enhanced stability due do reduced proteolysis. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418336 | 1 – 373 | mRNA-decapping enzyme subunit 2Add BLAST | 373 |
Proteomic databases
PaxDbi | Q8GW31 |
PRIDEi | Q8GW31 |
ProteomicsDBi | 224691 [Q8GW31-1] |
PTM databases
iPTMneti | Q8GW31 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
ExpressionAtlasi | Q8GW31, baseline and differential |
Genevisiblei | Q8GW31, AT |
Interactioni
Subunit structurei
Homodimer. Catalytic component of the decapping complex.
Interacts with DCP1, DCP5 and VCS.
3 PublicationsBinary interactionsi
Q8GW31
With | #Exp. | IntAct |
---|---|---|
At1g08370 [Q9SJF3] | 2 | EBI-4425465,EBI-7786643 |
GO - Molecular functioni
- protein homodimerization activity Source: TAIR
Protein-protein interaction databases
BioGRIDi | 16479, 4 interactors |
IntActi | Q8GW31, 3 interactors |
MINTi | Q8GW31 |
STRINGi | 3702.AT5G13570.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 106 – 234 | Nudix hydrolasePROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 233 – 250 | RNA bindingAdd BLAST | 18 | |
Regioni | 275 – 342 | DisorderedSequence analysisAdd BLAST | 68 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 139 – 160 | Nudix boxAdd BLAST | 22 | |
Motifi | 370 – 373 | PDZ-binding | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 275 – 292 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 293 – 322 | Basic and acidic residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 323 – 338 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2937, Eukaryota |
InParanoidi | Q8GW31 |
OMAi | KLNMSAI |
OrthoDBi | 1230808at2759 |
PhylomeDBi | Q8GW31 |
Family and domain databases
CDDi | cd03672, Dcp2p, 1 hit |
Gene3Di | 1.10.10.1050, 1 hit |
InterProi | View protein in InterPro IPR007722, DCP2_BoxA IPR036189, DCP2_BoxA_sf IPR044099, Dcp2_NUDIX IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF05026, DCP2, 1 hit PF00293, NUDIX, 1 hit |
SMARTi | View protein in SMART SM01125, DCP2, 1 hit |
SUPFAMi | SSF140586, SSF140586, 1 hit SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGLHRSSSS SKNIGNCLPS KELLDDLCSR FVLNVPEEDQ QSFERILFLV
60 70 80 90 100
EYAYWYYEDN AVENDPKLKS LSLKEFTSLL FNSCDVLRPY VTHIDDIFKD
110 120 130 140 150
FTSYKCRVPV TGAIILDETY ERCLLVKGWK GSSWSFPRGK KSKDEEDHAC
160 170 180 190 200
AIREVLEETG FDVSKLLKRE EYIEFVFRQQ RVRLYIVAGV TEDTVFAPLT
210 220 230 240 250
KKEISEITWH RLDHLQPASN EVITHGVSGL KLYMVAPFLS SLKSWILKHP
260 270 280 290 300
SPVARRPNKP LKALCVWNAR TSVGGNGTAT VESQNRKSEL RTTTMESNSR
310 320 330 340 350
KPELKRTTME SHSTKPELRK GTMESHNTTA TVESHNTKPV VDHSQDIKPG
360 370
GSFINFKFNQ SVILQALESG NSA
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044026 | 154 | E → ELSSAILLVNVAFQ in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006704 Genomic DNA Translation: BAB08683.1 Sequence problems. CP002688 Genomic DNA Translation: AED91912.1 CP002688 Genomic DNA Translation: AED91913.1 AK119112 mRNA Translation: BAC43684.1 BT005332 mRNA Translation: AAO63396.1 |
RefSeqi | NP_001190304.1, NM_001203375.1 [Q8GW31-2] NP_196861.2, NM_121360.4 [Q8GW31-1] |
Genome annotation databases
EnsemblPlantsi | AT5G13570.1; AT5G13570.1; AT5G13570 [Q8GW31-1] AT5G13570.2; AT5G13570.2; AT5G13570 [Q8GW31-2] |
GeneIDi | 831201 |
Gramenei | AT5G13570.1; AT5G13570.1; AT5G13570 [Q8GW31-1] AT5G13570.2; AT5G13570.2; AT5G13570 [Q8GW31-2] |
KEGGi | ath:AT5G13570 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006704 Genomic DNA Translation: BAB08683.1 Sequence problems. CP002688 Genomic DNA Translation: AED91912.1 CP002688 Genomic DNA Translation: AED91913.1 AK119112 mRNA Translation: BAC43684.1 BT005332 mRNA Translation: AAO63396.1 |
RefSeqi | NP_001190304.1, NM_001203375.1 [Q8GW31-2] NP_196861.2, NM_121360.4 [Q8GW31-1] |
3D structure databases
AlphaFoldDBi | Q8GW31 |
SMRi | Q8GW31 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 16479, 4 interactors |
IntActi | Q8GW31, 3 interactors |
MINTi | Q8GW31 |
STRINGi | 3702.AT5G13570.2 |
PTM databases
iPTMneti | Q8GW31 |
Proteomic databases
PaxDbi | Q8GW31 |
PRIDEi | Q8GW31 |
ProteomicsDBi | 224691 [Q8GW31-1] |
Genome annotation databases
EnsemblPlantsi | AT5G13570.1; AT5G13570.1; AT5G13570 [Q8GW31-1] AT5G13570.2; AT5G13570.2; AT5G13570 [Q8GW31-2] |
GeneIDi | 831201 |
Gramenei | AT5G13570.1; AT5G13570.1; AT5G13570 [Q8GW31-1] AT5G13570.2; AT5G13570.2; AT5G13570 [Q8GW31-2] |
KEGGi | ath:AT5G13570 |
Organism-specific databases
Araporti | AT5G13570 |
TAIRi | locus:2173174, AT5G13570 |
Phylogenomic databases
eggNOGi | KOG2937, Eukaryota |
InParanoidi | Q8GW31 |
OMAi | KLNMSAI |
OrthoDBi | 1230808at2759 |
PhylomeDBi | Q8GW31 |
Miscellaneous databases
PROi | PR:Q8GW31 |
Gene expression databases
ExpressionAtlasi | Q8GW31, baseline and differential |
Genevisiblei | Q8GW31, AT |
Family and domain databases
CDDi | cd03672, Dcp2p, 1 hit |
Gene3Di | 1.10.10.1050, 1 hit |
InterProi | View protein in InterPro IPR007722, DCP2_BoxA IPR036189, DCP2_BoxA_sf IPR044099, Dcp2_NUDIX IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF05026, DCP2, 1 hit PF00293, NUDIX, 1 hit |
SMARTi | View protein in SMART SM01125, DCP2, 1 hit |
SUPFAMi | SSF140586, SSF140586, 1 hit SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCP2_ARATH | |
Accessioni | Q8GW31Primary (citable) accession number: Q8GW31 Secondary accession number(s): F4K3Z9, Q9FNB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2012 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families