UniProtKB - Q8GTB6 (THCAS_CANSA)
Tetrahydrocannabinolic acid synthase
THCAS
Functioni
Oxidoreductase involved in the biosynthesis of cannabinoids-related terpenophenolic natural products, which have pharmacological activity (PubMed:15190053, PubMed:17669365, Ref. 4).
Catalyzes the oxidative cyclization of the monoterpene moiety in cannabigerolic acid (CBGA), producing delta9-tetrahydrocannabinolate (THCA), the major cannabioid in drug-type Cannabis plants (PubMed:15190053, PubMed:17669365, Ref. 4).
Can also use cannabinerolic acid as substrate, but not cannabigerol or cannabinerol (PubMed:15190053, PubMed:17669365, Ref. 4).
3 PublicationsMiscellaneous
Catalytic activityi
Cofactori
Activity regulationi
Kineticsi
- KM=134 µM for cannabigerolic acid2 Publications
- KM=254 µM for cannabinerolic acid2 Publications
- Vmax=2.68 nmol/sec/mg enzyme with cannabigerolic acid as substrate2 Publications
- Vmax=0.37 nmol/sec/mg enzyme with cannabinerolic acid as substrate2 Publications
pH dependencei
: terpenoid biosynthesis Pathwayi
This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 120 | FADCombined sources1 Publication | 1 | |
Binding sitei | 176 | FADCombined sources1 Publication | 1 | |
Binding sitei | 190 | FAD; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 236 | FADCombined sources1 Publication | 1 | |
Binding sitei | 241 | FAD; via amide nitrogen and carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 292 | Cannabigerolate1 Publication | 1 | |
Binding sitei | 417 | Cannabigerolate1 Publication | 1 | |
Binding sitei | 442 | CannabigerolateBy similarity | 1 | |
Active sitei | 484 | Proton acceptor1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 109 – 115 | FADCombined sources1 Publication | 7 | |
Nucleotide bindingi | 180 – 184 | FADCombined sources1 Publication | 5 | |
Nucleotide bindingi | 481 – 483 | FADCombined sources1 Publication | 3 |
GO - Molecular functioni
- Delta9-tetrahydrocannabinolate synthase activity Source: UniProtKB-EC
- FAD binding Source: InterPro
GO - Biological processi
- cannabinoid biosynthetic process Source: UniProtKB
- terpenoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BRENDAi | 1.21.3.7, 1159 |
UniPathwayi | UPA00213 |
Names & Taxonomyi
Protein namesi | Recommended name: Tetrahydrocannabinolic acid synthase1 Publication (EC:1.21.3.73 Publications)Short name: THCA synthase1 Publication Alternative name(s): Delta(1)-tetrahydrocannabinolic acid synthase1 Publication |
Gene namesi | Name:THCAS1 Publication |
Organismi | Cannabis sativa (Hemp) (Marijuana) |
Taxonomic identifieri | 3483 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Rosales › Cannabaceae › Cannabis |
Subcellular locationi
Extracellular region or secreted
- apoplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Apoplast, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 92 | H → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 108 | R → A: Strongly reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 110 | R → A: Strongly reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 114 | H → A: Loss of FAD binding and loss of catalytic activity. 2 Publications | 1 | |
Mutagenesisi | 208 | H → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 292 | H → A: Strongly reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 417 | Y → F: Reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 442 | E → Q: Slightly reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 484 | Y → F: Loss of catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | 2 PublicationsAdd BLAST | 28 | |
ChainiPRO_0000421142 | 29 – 545 | Tetrahydrocannabinolic acid synthaseAdd BLAST | 517 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 37 ↔ 99 | Combined sources1 Publication | ||
Glycosylationi | 65 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Glycosylationi | 89 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Cross-linki | 114 ↔ 176 | 6-(S-cysteinyl)-8alpha-(pros-histidyl)-FAD (His-Cys)1 Publication | ||
Glycosylationi | 168 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Glycosylationi | 297 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 305 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 329 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Glycosylationi | 467 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Glycosylationi | 499 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotationCombined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Monomer.
2 PublicationsStructurei
Secondary structure
3D structure databases
SMRi | Q8GTB6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 77 – 251 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 175 |
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit |
InterProi | View protein in InterPro IPR012951, BBE IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR006094, Oxid_FAD_bind_N |
Pfami | View protein in Pfam PF08031, BBE, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MNCSAFSFWF VCKIIFFFLS FHIQISIANP RENFLKCFSK HIPNNVANPK
60 70 80 90 100
LVYTQHDQLY MSILNSTIQN LRFISDTTPK PLVIVTPSNN SHIQATILCS
110 120 130 140 150
KKVGLQIRTR SGGHDAEGMS YISQVPFVVV DLRNMHSIKI DVHSQTAWVE
160 170 180 190 200
AGATLGEVYY WINEKNENLS FPGGYCPTVG VGGHFSGGGY GALMRNYGLA
210 220 230 240 250
ADNIIDAHLV NVDGKVLDRK SMGEDLFWAI RGGGGENFGI IAAWKIKLVA
260 270 280 290 300
VPSKSTIFSV KKNMEIHGLV KLFNKWQNIA YKYDKDLVLM THFITKNITD
310 320 330 340 350
NHGKNKTTVH GYFSSIFHGG VDSLVDLMNK SFPELGIKKT DCKEFSWIDT
360 370 380 390 400
TIFYSGVVNF NTANFKKEIL LDRSAGKKTA FSIKLDYVKK PIPETAMVKI
410 420 430 440 450
LEKLYEEDVG AGMYVLYPYG GIMEEISESA IPFPHRAGIM YELWYTASWE
460 470 480 490 500
KQEDNEKHIN WVRSVYNFTT PYVSQNPRLA YLNYRDLDLG KTNHASPNNY
510 520 530 540
TQARIWGEKY FGKNFNRLVK VKTKVDPNNF FRNEQSIPPL PPHHH
Polymorphismi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 63 | I → L in strain: 001. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB057805 mRNA Translation: BAC41356.1 AB212829 Genomic DNA Translation: BAE48241.1 AB212832 Genomic DNA Translation: BAE48244.1 AB212834 Genomic DNA Translation: BAE48246.1 AB212835 Genomic DNA Translation: BAE48247.1 AB212837 Genomic DNA Translation: BAE48249.1 AB212838 Genomic DNA Translation: BAE48250.1 AB183698 Genomic DNA Translation: BAD82947.1 AB183699 Genomic DNA Translation: BAD82948.1 AB183700 Genomic DNA Translation: BAD82949.1 AB183701 Genomic DNA Translation: BAD82950.1 AB183702 Genomic DNA Translation: BAD82951.1 AB183703 Genomic DNA Translation: BAD82952.1 AB183704 Genomic DNA Translation: BAD82953.1 AB183705 Genomic DNA Translation: BAD82954.1 |
Genome annotation databases
KEGGi | ag:BAC41356 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB057805 mRNA Translation: BAC41356.1 AB212829 Genomic DNA Translation: BAE48241.1 AB212832 Genomic DNA Translation: BAE48244.1 AB212834 Genomic DNA Translation: BAE48246.1 AB212835 Genomic DNA Translation: BAE48247.1 AB212837 Genomic DNA Translation: BAE48249.1 AB212838 Genomic DNA Translation: BAE48250.1 AB183698 Genomic DNA Translation: BAD82947.1 AB183699 Genomic DNA Translation: BAD82948.1 AB183700 Genomic DNA Translation: BAD82949.1 AB183701 Genomic DNA Translation: BAD82950.1 AB183702 Genomic DNA Translation: BAD82951.1 AB183703 Genomic DNA Translation: BAD82952.1 AB183704 Genomic DNA Translation: BAD82953.1 AB183705 Genomic DNA Translation: BAD82954.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3VTE | X-ray | 2.75 | A | 28-545 | [»] | |
SMRi | Q8GTB6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:BAC41356 |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
BRENDAi | 1.21.3.7, 1159 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit |
InterProi | View protein in InterPro IPR012951, BBE IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR006094, Oxid_FAD_bind_N |
Pfami | View protein in Pfam PF08031, BBE, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | THCAS_CANSA | |
Accessioni | Q8GTB6Primary (citable) accession number: Q8GTB6 Secondary accession number(s): Q33DQ4, Q5NTX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2013 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families