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UniProtKB - Q8GSN1 (MOMT_CATRO)
Protein
Myricetin O-methyltransferase
Gene
N/A
Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Functioni
Methylates myricetin and dihydromyricetin at 2 sites. Inactive towards 16-hydroxytabersonine, the phenylpropanoids 5-hydroxyferulate, caffeate and their CoA-esters, flavones and flavanones possessing 2 or 3 B-ring hydroxyl groups.
1 PublicationCatalytic activityi
- S-adenosyl-L-methionine + a 3'-hydroxyflavonoid = S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid.1 Publication EC:2.1.1.267
- S-adenosyl-L-methionine + a 5'-hydroxy-3'-methoxyflavonoid = S-adenosyl-L-homocysteine + a 3',5'-dimethoxyflavonoid.1 Publication EC:2.1.1.267
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 189 | S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 212 | S-adenosyl-L-methioninePROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 232 | S-adenosyl-L-methioninePROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 233 | S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 246 | S-adenosyl-L-methioninePROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 250 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
GO - Molecular functioni
- laricitrin 5'-O-methyltransferase activity Source: UniProtKB-EC
- myricetin 3'-O-methyltransferase activity Source: UniProtKB
- protein dimerization activity Source: InterPro
GO - Biological processi
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.267, 1211 |
Names & Taxonomyi
Protein namesi | Recommended name: Myricetin O-methyltransferase (EC:2.1.1.267)Alternative name(s): CrOMT21 Publication Flavonoid O-methyltransferaseImported |
Organismi | Catharanthus roseus (Madagascar periwinkle) (Vinca rosea) |
Taxonomic identifieri | 4058 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthinae › Catharanthus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000405018 | 1 – 348 | Myricetin O-methyltransferaseAdd BLAST | 348 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 |
Post-translational modificationi
The N-terminus is blocked.1 Publication
Keywords - PTMi
AcetylationPTM databases
iPTMneti | Q8GSN1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.PROSITE-ProRule annotation
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8GSN1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELQSSEIRN AQAHFFTQVF SFTSMSSLKC AVQLGIPDAI HSHGKPMALS
60 70 80 90 100
DLTNSLPINP SKAPYIYRLM RILVAAGYFS EEEKNVYSLT PFTRLLLKND
110 120 130 140 150
PLNSISMVLG VNQIAELKAW NAMSEWFQNE DLTAFETAHG KNFWDFGAED
160 170 180 190 200
KYGKNFDGVM AADSILVSKM LIPEFNYLFE GLDSLVDVGG GTGTIAKAIA
210 220 230 240 250
KSFPDLKCTV FDLPHVVANL ESTENLEFVG GDMFEKIPSA NAILLKWILH
260 270 280 290 300
DWKDEECVKV LKMCRKAIPE KEKGGKVILI ETVLMDSKKH ENEEAVKAQI
310 320 330 340
SSDIDMMVFF TAKERTEEEW ATLFREAGFS GYKIFPMIDF RSPIEVYP
Mass spectrometryi
Molecular mass is 39191±3 Da. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY127568 Genomic DNA Translation: AAM97497.1 |
Genome annotation databases
KEGGi | ag:AAM97497 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY127568 Genomic DNA Translation: AAM97497.1 |
3D structure databases
SMRi | Q8GSN1 |
ModBasei | Search... |
PTM databases
iPTMneti | Q8GSN1 |
Genome annotation databases
KEGGi | ag:AAM97497 |
Enzyme and pathway databases
BRENDAi | 2.1.1.267, 1211 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR012967, Plant_MeTrfase_dimerisation IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF08100, Dimerisation, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MOMT_CATRO | |
Accessioni | Q8GSN1Primary (citable) accession number: Q8GSN1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 8, 2011 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 73 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families